Biochimica et biophysica acta. Biomembranes最新文献_第9页

Structural modification of lipid membranes by polyphenols: A fluorescence spectroscopy study 用多酚修饰脂质膜的结构：荧光光谱研究。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-03-01 Epub Date: 2025-01-22 DOI: 10.1016/j.bbamem.2025.184411

Valeriya M. Trusova , Uliana K. Tarabara , Mette H. Thomsen , Galyna P. Gorbenko

{"title":"Structural modification of lipid membranes by polyphenols: A fluorescence spectroscopy study","authors":"Valeriya M. Trusova , Uliana K. Tarabara , Mette H. Thomsen , Galyna P. Gorbenko","doi":"10.1016/j.bbamem.2025.184411","DOIUrl":"10.1016/j.bbamem.2025.184411","url":null,"abstract":"<div><div>The present study investigates the molecular mechanisms of polyphenol-lipid interactions and their impact on membrane properties. Using pyrene and DPH as reporter molecules, we examined the impact of quercetin, curcumin, gallic, and salicylic acids on membranes composed of phosphatidylcholine (PC) and its mixtures with phosphatidylglycerol (PG), cardiolipin (CL), and cholesterol (Chol). Quercetin was found to increase the lipid order without affecting the lipid bilayer free volume, indicating interactions near the membrane surface. In turn, curcumin exhibited more complex effects, reducing free volume in PC but increasing it in PG vesicles, reflecting its amphiphilic structure and variable penetration depth. Gallic and salicylic acids selectively increased free volume at the membrane core without influencing lipid order at the upper regions of lipid bilayer. The results obtained demonstrate that polyphenol structure and lipid composition dictate the resultant pattern of polyphenol-membranes interactions, which may have implications for drug delivery and nutraceutical design.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184411"},"PeriodicalIF":2.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the structure and function of a membrane-active plant protein domain using in silico mutagenesis 阐明膜活性植物蛋白结构域在硅诱变中的结构和功能。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1016/j.bbamem.2025.184409

Lennie K.Y. Cheung , Sebastian Thallmair , Rickey Y. Yada

{"title":"Elucidating the structure and function of a membrane-active plant protein domain using in silico mutagenesis","authors":"Lennie K.Y. Cheung , Sebastian Thallmair , Rickey Y. Yada","doi":"10.1016/j.bbamem.2025.184409","DOIUrl":"10.1016/j.bbamem.2025.184409","url":null,"abstract":"<div><div>The <em>Solanum tuberosum</em> (common potato) plant specific insert (<em>St</em>PSI) is an antimicrobial protein domain that exhibits membrane-disrupting and membrane-fusing activity upon dimerization at acidic pH, activity proposed to involve electrostatic attraction and membrane anchoring mediated by specific positively-charged and conserved tryptophan residues, respectively. This study is the first to employ an in silico mutagenesis approach to clarify the structure-function relationship of a plant specific insert (PSI), where ten rationally-mutated <em>St</em>PSI variants were investigated using all-atom and coarse-grained molecular dynamics. The tryptophan (W) residue at position 18 (W18) of wild-type <em>St</em>PSI was predicted to confer structural flexibility to the dimer and mediate a partial separation of the assembled monomers upon bilayer contact, while residues including W77 and the lysine (K) residue at position 83 (K83) were predicted to stabilize secondary structure and influence association with the model membrane. Mechanisms predicted to influence <em>St</em>PSI-membrane association included the partial separation of assembled monomers on the bilayer surface, formation of a specific salt bridge, and membrane anchoring of hinge 2 residues. The findings suggested that the structure-function relationship of <em>St</em>PSI involved several mechanisms that may each be modulated by specific key residues, insights that may support efforts to develop PSI with tailored membrane association for novel applications in plant biotechnology and crop protection.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184409"},"PeriodicalIF":2.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential effects of endo- and exopolyphosphatase expression on the induction of the mitochondrial permeability transition pore 内多磷酸酶和外多磷酸酶表达对线粒体通透性过渡孔诱导的差异影响。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1016/j.bbamem.2025.184408

Yaw Akosah , Vedangi Hambardikar , Maria Neginskaya , Sally Morris , Maria E. Solesio , Evgeny V. Pavlov

{"title":"Differential effects of endo- and exopolyphosphatase expression on the induction of the mitochondrial permeability transition pore","authors":"Yaw Akosah , Vedangi Hambardikar , Maria Neginskaya , Sally Morris , Maria E. Solesio , Evgeny V. Pavlov","doi":"10.1016/j.bbamem.2025.184408","DOIUrl":"10.1016/j.bbamem.2025.184408","url":null,"abstract":"<div><div>Inorganic polyphosphate (polyP) is a polymer that consists of a series of orthophosphates connected by high-energy phosphoanhydride bonds, like those found in ATP. In mammalian mitochondria, polyP has been linked to the activation of the mitochondrial permeability transition pore (mPTP). However, the details of this process are not completely understood. The activation of mPTP by polyP may involve the regulation of bioenergetics, Ca<sup>2+</sup> buffering, or direct involvement in mPTP channel formation. In this study, using refractive index imaging techniques, we examined mPTP induction in both wild-type (WT) SH-SY5Y cells, and mutant SH-SY5Y cells expressing either mitochondrially targeted exopolyphosphatase (MitoPPX), which depletes polyP by breaking free terminal phosphoanhydride bonds; or endopolyphosphatase (MitoPPN), which cleaves internal phosphoanhydride bonds and thus can target polyP pool with protected terminal groups. Upon treating the cells with the calcium ionophore ferutinin, the influx of Ca<sup>2+</sup> triggered mitochondrial membrane depolarization and permeabilization in both WT and MitoPPX cells indicating activation of mPTP. However, in MitoPPN cells, mitochondrial depolarization occurred without mPTP activation. Based on these findings we propose the possibility that activation of mPTP is not linked to the pool of free polyP. This supports the hypothesis that polyP is either an important structural component of the mPTP channel or associated with other macromolecular complexes involved in mPTP induction.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184408"},"PeriodicalIF":2.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lock and key: Quest to find the most compatible membrane mimetic for studying membrane proteins in native environment 锁和钥匙：寻找最兼容的膜模拟物来研究天然环境中的膜蛋白。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-03-01 Epub Date: 2025-02-04 DOI: 10.1016/j.bbamem.2025.184414

Rahul Yadav , Debarghya Pratim Gupta , Chandan Singh

{"title":"Lock and key: Quest to find the most compatible membrane mimetic for studying membrane proteins in native environment","authors":"Rahul Yadav , Debarghya Pratim Gupta , Chandan Singh","doi":"10.1016/j.bbamem.2025.184414","DOIUrl":"10.1016/j.bbamem.2025.184414","url":null,"abstract":"<div><div>Membrane proteins play crucial roles in cellular signal transduction, molecule transport, host-pathogen interactions, and metabolic processes. However, mutations, changes in membrane properties, and environmental factors can lead to loss of protein function. This results in impaired ligand binding and misfolded structures that prevent proteins from adopting their native conformation. Many membrane proteins are also therapeutic targets in various diseases, where drugs can either restore or inhibit their specific functions. Understanding membrane protein structure and function is vital for advancing cell biology and physiology. Experimental studies often involve extracting proteins from their native environments and reconstituting them in membrane mimetics like detergents, bicelles, amphipols, nanodiscs, and liposomes. These mimetics replicate aspects of native membranes, aiding in the study of protein behavior outside living cells. Scientists continuously explore new, more native-like membrane mimetics to improve experimental accuracy. This dynamic field involves evaluating the advantages and disadvantages of different mimetics and optimizing the reconstitution process to better mimic natural conditions.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184414"},"PeriodicalIF":2.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipopolysaccharide supramolecular organization regulates the activation of coagulation factor XII 脂多糖超分子组织调控凝血因子XII的活化

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-03-01 Epub Date: 2025-02-06 DOI: 10.1016/j.bbamem.2025.184415

André L. Lira , Ting Liu , Joseph E. Aslan , Cristina Puy , Owen J.T. McCarty

引用次数: 0

The effect of oxidative stress on the adenosine A2A receptor activity and signalling 氧化应激对腺苷A2A受体活性及信号传导的影响。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-03-01 Epub Date: 2025-02-03 DOI: 10.1016/j.bbamem.2025.184412

Idoia Company-Marín , Joseph Gunner , David Poyner , John Simms , Andrew R. Pitt , Corinne M. Spickett

{"title":"The effect of oxidative stress on the adenosine A2A receptor activity and signalling","authors":"Idoia Company-Marín , Joseph Gunner , David Poyner , John Simms , Andrew R. Pitt , Corinne M. Spickett","doi":"10.1016/j.bbamem.2025.184412","DOIUrl":"10.1016/j.bbamem.2025.184412","url":null,"abstract":"<div><div>The adenosine A<sub>2A</sub> receptor (A<sub>2A</sub>R) is a G-protein coupled receptor that has important anti-inflammatory effects in response to some agonists and consequently is considered a therapeutic target. Its activity is affected by local membrane lipid environment and presence of certain phospholipid classes, so studies should be conducted using extraction methods such as styrene maleic acid <em>co</em>-polymers (SMA) that retain the local lipids. Currently, little is known about the effect of oxidative stress, which may arise from inflammation, on the A<sub>2A</sub>R. Therefore, it was over-expressed in <em>Pichia pastoris</em>, SMA was used to extract the A<sub>2A</sub>R from cell membranes and its response to ligands was tested in the presence or absence of the radical initiator AAPH or reactive aldehyde acrolein. SMA-extracted A<sub>2A</sub>R was able to undergo conformational changes, measured by tryptophan fluorescence, in response to its ligands but oxidative treatments had no effect on the structural changes. Similarly, the treatments did not affect temperature-dependent protein unfolding. In contrast, in HEK293 cells expressing the A<sub>2A</sub>R, oxidative treatments increased cAMP levels in response to the agonist NECA but had no effect on direct activation of adenylate cyclase. Thus, oxidative stress may be a homeostatic mechanism that abrogates inflammation via the A<sub>2A</sub>R signalling pathway.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184412"},"PeriodicalIF":2.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human cathelicidin LL-37 rapidly disrupted colonic epithelial integrity 人cathelicidin LL-37迅速破坏结肠上皮的完整性。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-03-01 Epub Date: 2025-01-19 DOI: 10.1016/j.bbamem.2025.184410

Geeta Kilari, Jacquelyn Tran, Graham A.D. Blyth, Eduardo R. Cobo

{"title":"Human cathelicidin LL-37 rapidly disrupted colonic epithelial integrity","authors":"Geeta Kilari, Jacquelyn Tran, Graham A.D. Blyth, Eduardo R. Cobo","doi":"10.1016/j.bbamem.2025.184410","DOIUrl":"10.1016/j.bbamem.2025.184410","url":null,"abstract":"<div><div>The intestinal barrier, held together by epithelial cells and intercellular tight junction (TJ) proteins, prevents the penetration of microbial pathogens. Concurrently, intestinal epithelial cells secrete antimicrobial peptides, including cathelicidin. Cathelicidin has direct antibacterial and immunomodulatory functions, although its role in intestinal integrity remains elusive. In this study, we demonstrate that direct stimulation of human colonic epithelial (T84) cells with human cathelicidin, LL-37, resulted in a rapid and transient increase in epithelial cell permeability. This increased permeability was associated with the TJ proteins occludin and claudin-2 degradation, mediated by these specific proteins' endocytosis and lysosomal degradation. While murine cathelicidin (CRAMP) failed to modify T84 cell permeability, LL-37 degraded TJ proteins in murine rectal epithelial (CMT-93) cells. The stimulus of (CMT-93) cells with LL-37 aggravated the cell permeability and furthered TJ degradation provoked by the intestinal pathogen, attaching/effacing (A/E) <em>Citrobacter rodentium</em> (<em>C. rodentium</em>). The number of <em>C. rodentium</em> that colonized CMT-93 cells was not severely impacted by the presence of LL-37. While a temporary disruption of tight junctions by LL-37 may lead to a ‘leaky gut,’ this study demonstrates that LL-37 increases epithelial cell permeability by degrading TJ proteins occludin and claudin-2 through endocytosis and lysosomal degradation. These immunomodulatory actions occurring at concentrations lower than those microbicidal uncover a new guise for cathelicidin modulating the epithelial barrier against A/E pathogens. Recognizing native cathelicidin's functions in a specified disease setting (e.g., colitis) will help establish it as an anti-infectious immunomodulator.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184410"},"PeriodicalIF":2.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simulation analysis of the effect of a cholesterol-dependent fusogenic peptide from HIV gp41 on membrane phospholipid dynamics HIV gp41中胆固醇依赖性融合原肽对膜磷脂动力学影响的模拟分析。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-03-01 Epub Date: 2025-02-01 DOI: 10.1016/j.bbamem.2025.184413

Manami Nishizawa , Kazuhisa Nishizawa

{"title":"A simulation analysis of the effect of a cholesterol-dependent fusogenic peptide from HIV gp41 on membrane phospholipid dynamics","authors":"Manami Nishizawa , Kazuhisa Nishizawa","doi":"10.1016/j.bbamem.2025.184413","DOIUrl":"10.1016/j.bbamem.2025.184413","url":null,"abstract":"<div><div>CpreTM is a fusogenic peptide whose N-terminal portion is derived from the membrane-proximal external region (MPER) and C-terminal portion covers the transmembrane (TM) domain of gp41 of HIV. CpreTM has been shown to induce membrane fusion, which requires cholesterol molecules as membrane components. To gain insight into the effects of CpreTM on membrane lipid dynamics, we performed molecular dynamics simulations. In conventional simulations, several cholesterol-binding sites were found under the segment derived from MPER and near the cholesterol recognition/interaction amino acid consensus (CRAC) motif located at the C-terminus of MPER. CpreTM resides in shallower positions in the POPC (palmitoyl oleoyl phosphatidylcholine)/cholesterol bilayer than in the pure POPC bilayer. Our metadynamic simulations using the position of one POPC molecule (“target POPC”) as the collective variable showed that CpreTM remarkably lowered the free energy cost for the POPC protrusion from the cholesterol-containing membrane; e.g., the cost for 0.7 nm outward displacement from the height of bulk POPC molecules was decreased by ~10 kJ/mol compared to the peptide-free corresponding system. Such stabilization of the POPC protrusion was not observed in the cholesterol-free POPC membrane. It was more pronounced near the aromatic residues, including the three Trp residues of CpreTM, suggesting important roles for aromatic residues in stabilizing the POPC protrusion.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184413"},"PeriodicalIF":2.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the antimicrobial potential of the peptide CIDEM-501 through acylation: A computational approach 通过酰化提高肽CIDEM-501的抗菌潜力：一种计算方法。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1016/j.bbamem.2025.184407

Daniel Alpízar-Pedraza , Adrian Romero-Rivero , Rolando Perdomo-Morales , Niurys Mantilla-García , Claudia Pérez-Martínez , Hilda Garay-Pérez , Frank Rosenau , Ludger Ständker , Vivian Montero-Alejo

{"title":"Improving the antimicrobial potential of the peptide CIDEM-501 through acylation: A computational approach","authors":"Daniel Alpízar-Pedraza , Adrian Romero-Rivero , Rolando Perdomo-Morales , Niurys Mantilla-García , Claudia Pérez-Martínez , Hilda Garay-Pérez , Frank Rosenau , Ludger Ständker , Vivian Montero-Alejo","doi":"10.1016/j.bbamem.2025.184407","DOIUrl":"10.1016/j.bbamem.2025.184407","url":null,"abstract":"<div><div>Acylation is a common method used to modify antimicrobial peptides to enhance their effectiveness. It increases the interactions between the peptide and the bacterial cell membranes. However, acylation can also reduce the selectivity of the peptides by making them more active on eukaryotic membranes, which can lead to unintended toxicity. This study examines the potential of using <em>in silico</em> tools to evaluate the interaction and selectivity of the antimicrobial peptide CIDEM-501 when acylated with decanoic acid at the N-terminus, compared to the non-acylated counterpart. Circular dichroism, microdilution, and hemolysis assays were used to determine the peptide's secondary structure, antimicrobial activity, and selectivity to validate the theoretical predictions. The acylated peptide showed a more stable interaction with the bacterial membrane by inserting the acyl chain into the membrane's hydrophobic core, which led to tighter adsorption and a greater buried surface area. Additionally, it significantly altered membrane order more than the non-acylated counterpart, suggesting superior antimicrobial potential. Finally, <em>in vitro</em> activity assays confirmed theoretical predictions, showing that the acylated peptide had lower Minimum Inhibitory Concentration (MIC) values than the non-acylated peptide. Neither peptide showed significant hemolytic activity at their MIC. The computational techniques used in this study displayed strong predictive capability and helped to elucidate the interaction between the peptide and the membranes.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 3","pages":"Article 184407"},"PeriodicalIF":2.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142943679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuning expression of GPCRs for the secretory pathway in the baculovirus-insect cell expression system 在杆状病毒-昆虫细胞表达系统中调节分泌途径 GPCR 的表达。

IF 2.8 3区生物学

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-02-01 Epub Date: 2024-10-28 DOI: 10.1016/j.bbamem.2024.184397

Jakob Aastrup Jørgensen

{"title":"Tuning expression of GPCRs for the secretory pathway in the baculovirus-insect cell expression system","authors":"Jakob Aastrup Jørgensen","doi":"10.1016/j.bbamem.2024.184397","DOIUrl":"10.1016/j.bbamem.2024.184397","url":null,"abstract":"<div><div>The overexpression of G-protein-coupled receptors (GPCRs) remains one of the biggest hurdles for structural studies of these proteins. To date, the most usually applied system for this task is the insect cell/baculovirus expression system. A drawback of this system, however, is the accumulation of protein that is resistant to solubilization with the commonly used mild detergent DoDecylMaltoside (DDM). In addition, poor surface expression is often observed. In this study, it is shown how an earlier AcMNPV 39K promoter, can express receptors that are found primarily on the cell membrane, as revealed by confocal microscopy, and the protein can be solubilized to a higher degree by DDM in a less aggregation-prone form, as monitored by fluorescence size-exclusion chromatography. In addition, a strong effect on the yield was observed when the AcMNPV gp67 signal sequence was used. The documentation of the 39K promoter as an improvement over the frequently used polyhedrin promoter, along with the effect of the gp67 signal sequence are important steps toward ultimately improving the expression in terms of total functional yield, while also shedding light on the nature of the process of overproduction of membrane proteins, in particular, GPCRs.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"1867 2","pages":"Article 184397"},"PeriodicalIF":2.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142543407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0