Benha Veterinary Medical Journal最新文献

In Vivo synergistic effect of curcumin in combination with marbofloxacin against E coli infection in rats 姜黄素与马勃沙星联用对大鼠大肠杆菌感染的体内协同作用

Benha Veterinary Medical Journal Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.282638.1806

Rania Abdelrazek Mohammed Ahmed, M. Elsayed, Enas Farag, Mohamed Aboubakr

{"title":"In Vivo synergistic effect of curcumin in combination with marbofloxacin against E coli infection in rats","authors":"Rania Abdelrazek Mohammed Ahmed, M. Elsayed, Enas Farag, Mohamed Aboubakr","doi":"10.21608/bvmj.2024.282638.1806","DOIUrl":"https://doi.org/10.21608/bvmj.2024.282638.1806","url":null,"abstract":"Keywords This study aimed to evaluate the potential synergistic effect of curcumin in combination with marbofloxacin against E coli infection in rats. Twenty-five male rats were segregated equally into five groups of Wister albino rats; Group (1) acted as the control group (normal saline only, 0.2 ml); Group (2) was given E. Coli . Group (3) included rats infected with E. coli, and were administered an oral dose of marbofloxacin (5 mg/kg b.wt) for 5 days; Group (4) also administered the same oral dose of marbofloxacin daily for 5 days. . Group (5) rats were treated to E. coli by a single intraperitoneal injection of 2 ml of (1 × 10 9 ) of colony forming units/ml. Following that, rats were received oral dosages of both marbofloxacin and curcumin (20 mg/kg b.wt) Serum biochemical analysis revealed that in marbofloxacin treated group, albumin levels were significantly (P<0.05) decreased mean while transaminases (ALT and AST), alkaline phosphatase (ALP), total bilirubin, triglycerides, and cholesterol were significantly increased during marbofloxacin treatment. On the other hand, rats treated with marbofloxacin and curcumin showed noticeably decreased levels of triglycerides, cholesterol, and transaminases (ALT and AST), and significantly increased levels of albumin compared to rats that received marbofloxacin only. Histopathological examinations of liver tissue revealed severe liver damage in E.coli infected rats and counteracted by marbofloxacin and more treated through curcumin combination. In conclusion, the administration of curcumin in combination with marbofloxacin had a potential synergistic effect on the liver damage caused by E. coli infection in rats.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"13 33","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141699961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A potential therapeutic impact of Gallic acid in a rat model of hepatocarcinogenesis through inhibition of cell proliferation and oncogenic miRNA-221 and induction of apoptosis by Nrf-2 /Bcl-2/TGF- β1 signaling pathways 没食子酸通过抑制细胞增殖和致癌 miRNA-221 以及通过 Nrf-2 /Bcl-2/TGF- β1 信号通路诱导细胞凋亡，对大鼠肝癌模型具有潜在的治疗作用

Benha Veterinary Medical Journal Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.279991.1802

Aʼlaa El-Sayed Al-Semelawy, Samy Ali Aziza, Hussein Abd El-Maksoud, Y. A. El-Senosi, Afaf Desoky Abel Magid

引用次数: 0

Molecular Docking analysis of the antibacterial effect of pomegranate (Punica granatum) fruit molasses bioactive compounds 石榴（Punica granatum）果实糖蜜生物活性化合物抗菌效果的分子对接分析

Benha Veterinary Medical Journal Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.284813.1816

Hanaa S. Bekeir, Ahmed Hamad, Nesreen Z. Eleiwa, reham A. Amin

{"title":"Molecular Docking analysis of the antibacterial effect of pomegranate (Punica granatum) fruit molasses bioactive compounds","authors":"Hanaa S. Bekeir, Ahmed Hamad, Nesreen Z. Eleiwa, reham A. Amin","doi":"10.21608/bvmj.2024.284813.1816","DOIUrl":"https://doi.org/10.21608/bvmj.2024.284813.1816","url":null,"abstract":"Keywords Pomegranate molasses is a functional element that has generated extensive scientific attention in the search for effective bioactive natural compounds. The in-silico effects of pomegranate molasses on foodborne pathogens were investigated using molecular docking analysis, using the crystal structures of E. coli topoisomerase II DNA gyrase B as a target. On the ATP-active pocket of E. coli DNA gyrase, the binding affinities and interactions of ellagic acid, gallic acid, punicalagin, and punicalin were examined. According to the findings, novobiocin had a docking score of -6.30 Kcal/mol, whereas the bioactive components of pomegranate molasses (punicalagin, punicalin, gallagic acid, and ellagic acid) adopted the best binding style with a score of -7.2 to -9.8 Kcal/mol. Our findings suggested that pomegranate molasses could provide powerful antibacterial agents and DNA gyrase inhibitors via unique structural properties that address antimicrobial resistance. They are being investigated further as a natural biocontrol agent. Future research to investigate the actual effects of pomegranate fruit molasses against E. coli using both plate inhibition assays and different","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141714040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A field investigation of the impact of the interaction between IBR vaccination and insemination time in cow herd: Reproductive and economic perspectives 对牛群中 IBR 疫苗接种与授精时间之间相互作用影响的实地调查：繁殖和经济角度

Benha Veterinary Medical Journal Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.293231.1830

Wesam Yousef Kamel, Amira M. Abd-El Hamed, Ehab ElNahas, Mahmoud E.A. AbouElRoos, G. Sosa, Mohamed M. M. Kandiel

引用次数: 0

Biofilm formation and regulating genes of Salmonella enterica recovered from chicken and fish samples. 从鸡肉和鱼肉样本中提取的肠炎沙门氏菌的生物膜形成和调控基因。

Benha Veterinary Medical Journal Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.287159.1821

Ahmed Hamad, Faten S. Hassanin, A. Salem, reham A. Amin

{"title":"Biofilm formation and regulating genes of Salmonella enterica recovered from chicken and fish samples.","authors":"Ahmed Hamad, Faten S. Hassanin, A. Salem, reham A. Amin","doi":"10.21608/bvmj.2024.287159.1821","DOIUrl":"https://doi.org/10.21608/bvmj.2024.287159.1821","url":null,"abstract":"Keywords The study sought to evaluate the biofilm formation capacity and biofilm-related gene profiles of Salmonella enterica recovered from food of animal origins. A total of 24 Salmonella enterica strains were isolated from 215 samples collected from food of animal origins (Raw chicken breast (45 samples), breaded chicken product (45 samples), shrimp (25 samples), Mackerel fish (25 samples), sea bass fillet (25 samples), tilapia fillet (25 samples), and mugil fillet (25 samples)) in Al Qalyubia Governorate, Egypt, during 2020– 2022. Using the crystal violet quantitative microtiter plate method and Congo red agar plating, the isolates' ability to form biofilms was evaluated. Polymerase Chain Reaction analysis was used to identify the presence of four biofilm-related genes ( csgA, csgD, sdiA, and sipA ) in strains that were highly capable of forming biofilms. The results revealed that 62.50% (15/24) of the strains exhibited strong biofilm formation, 20.83% (5/24) were moderate biofilm producers, and 16.67 % (4/24) were weak biofilm producers. Over 80% of the Salmonella enterica strains that are known to produce strong biofilm were found to be positive for all four of the biofilm-related genes when the strains were analyzed. The current study findings underscored the importance of developing effective preventive and control techniques to address the issues posed by biofilm formation in the food industry.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"23 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141717131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of fish quality in Alexandria small-scale fisheries: A value chain analysis 确定亚历山大小型渔业的鱼类质量：价值链分析

Benha Veterinary Medical Journal Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.260284.1772

sally Ebrahem Abdelaziz, Mai Salah, Haiam M. Aboul-Ela, Reda Fahim

{"title":"Determination of fish quality in Alexandria small-scale fisheries: A value chain analysis","authors":"sally Ebrahem Abdelaziz, Mai Salah, Haiam M. Aboul-Ela, Reda Fahim","doi":"10.21608/bvmj.2024.260284.1772","DOIUrl":"https://doi.org/10.21608/bvmj.2024.260284.1772","url":null,"abstract":"Keywords The Egyptian Mediterranean fisheries sector is a critical contributor to the country's economy, with fish exports accounting to approximately 40% of the total agricultural exports. This sector also plays a vital role in providing employment opportunities, particularly for those living along the Mediterranean coast. However, the sector has been facing various challenges that threaten its sustainability, including overfishing, illegal fishing practices, and environmental degradation. A value chain analysis can help to identify the various stages of the fisheries sector and their interdependencies. In this study, four value chain stages (vessels, wholesalers, retailers, and hypermarkets) were conducted in Alexandria, Egypt, for particular 30 fish species ( Mullus barbatus, Sardinella aurita, and Diplodussargus ). To conduct the value chain study in the sampling areas of small-scale fisheries, the following parameters were assessed: total volatile nitrogen (TVN), thiobarbituric acid (TBA), and pH, The results of the TBA tests and TVN analysis revealed notable disparities between the stages. These tests were frequently used to estimate the quality and freshness of fish, and their findings showed that samples with higher concentrations of these substances had more failures. Conclusively, the current study recommended stricter regulations on harvest size restrictions, monitoring programs, certification procedures, postharvest facilities, seasonal closures, social enterprises, finance facilities, and habitat protection as suggested by applying value chain analysis in small-scale fisheries management.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141699190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and critical antibiotic-resistance traits of Bacillus cereus and Staphylococcus aureus isolated from raw and ready-to-eat meat products 从生肉制品和即食肉制品中分离出的蜡样芽孢杆菌和金黄色葡萄球菌的流行率和关键抗生素耐药性特征

Benha Veterinary Medical Journal Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.283334.1809

Eman Masood, F. Mohamed, Nahla Abo EL-Roos, I. Sabike

{"title":"Prevalence and critical antibiotic-resistance traits of Bacillus cereus and Staphylococcus aureus isolated from raw and ready-to-eat meat products","authors":"Eman Masood, F. Mohamed, Nahla Abo EL-Roos, I. Sabike","doi":"10.21608/bvmj.2024.283334.1809","DOIUrl":"https://doi.org/10.21608/bvmj.2024.283334.1809","url":null,"abstract":"Keywords The study aimed to compare Bacillus cereus ( B. cereus ) and Staphylococcus aureus (S . aureus ) prevalence and antimicrobial resistance profiles in raw and ready-to-eat meat products (RTEM). One hundred samples of raw hamburger, sausage, RTEM hawawshi, and kofta were tested using standard culture and automated VITEK2 methods. PCR was used to detect resistance genetic components. The overall prevalence of S. aureus and B. cereus was 20%, and 14%, respectively. Staphylococcus aureus and B. cereus were isolated at similarly high rates (60.0% and 73.7%, respectively) from raw meat products. Five percent (1/20) of the B . cereus isolates detected in raw burger samples were multidrug-resistant (MDR), whereas 25 % of S . aureus were MDR, with three resistance patterns. Neither the mcr 1 nor the van A genes were found in the B . cereus . While nor A was found in four isolates, both bla TEM and bla CTX were found in three. The bla SHV was found only in three raw-derived isolates, two of which also shared bla TEM , bla CTX , and nor A or bla TEM and nor A. The data revealed that all of the MDR S . aureus isolates tested positive for mec A but not van A genes. Such pathogens in RTE meat with genes confer resistance to key antibiotics, endangering public health and hastening the emergence of superbugs.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"277 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141712703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical profile of Salted and Smoked Fish 咸鱼和熏鱼的化学成分概况

Benha Veterinary Medical Journal Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.238557.1729

Yomna Soliman, Walid Arab, A. Edris, Amina Elrais

引用次数: 0

Molecular characterization of isolated duck viral hepatitis and its pathogenicity in one day and seven day old ducklings in Qalyubia governorate. 卡留比亚省一天龄和七天龄雏鸭分离出的鸭病毒性肝炎的分子特征及其致病性。

Benha Veterinary Medical Journal Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.279397.1800

Aya Hamdy Behery, K. Zyan, Ahmed Eissa Saad, A. El-Shemy

引用次数: 0

The effect of a 3D-modeled pyramidal packing shape on the growth of foodborne pathogens inoculated in ribeye-lion at dynamic room temperatures. 三维建模的金字塔填料形状对动态室温下接种到肋眼石狮中的食源性病原体生长的影响。

Benha Veterinary Medical Journal Pub Date : 2024-07-01 DOI: 10.21608/bvmj.2024.270643.1789

Samaa Yehya, Shimaa N. Edris, Ahmed Hamad, Islam I. Sabeq

{"title":"The effect of a 3D-modeled pyramidal packing shape on the growth of foodborne pathogens inoculated in ribeye-lion at dynamic room temperatures.","authors":"Samaa Yehya, Shimaa N. Edris, Ahmed Hamad, Islam I. Sabeq","doi":"10.21608/bvmj.2024.270643.1789","DOIUrl":"https://doi.org/10.21608/bvmj.2024.270643.1789","url":null,"abstract":"Keywords Food packaging serves several important tasks, including preserving and controlling foodborne pathogens. One component that contributes significantly to these functions is the design of the food packaging itself. Bacterial retardation of natural meat biota was demonstrated using pyramidal packing patterns designed by the Great Pyramid's dimension ratios at constant chilling temperatures. The current study, based on the Giza-pyramid dimension ratio, used dynamic room temperature to evaluate the consequences of pyramidal packaging on minced ribeye meat experimentally contaminated with foodborne pathogens: Listeria monocytogenes and Salmonella Typhimurium , as well as naturally occurred Escherichia coli . The pyramidal growth curves were compared to cuboidal and cylindrical 3D-printed containers. The APC count increased marginally in all three forms with time, but declined considerably in pyramidal stored beef at 8, 14, 18, and 24 hours and circular stored beef at 32, 48 hours (P < 0.05). The result revealed that the packaging shape had no significant effect on the growth curves of both S. Typhimurium and E. coli . The pyramidal packaging negatively impacted the L. monocytogens growth curve, which was more obvious at the end of the storage period than other packaging shapes. The current study was conducted at dynamic room temperature, which may counteract pyramidal effect noticed when storage occurred at constant temperature. Also, the delayed or non-significant impact of the pyramidal package observed here on growth curves of the inoculated pathogen, particularly the Gram-negative one, could be attributable to other factors such as type of packaging material magnetic field stability. Conclusively, the hierarchical shape had optimal and efficient effects on the control of microbial growth introduced in minced meat therefore, it essential to consider incorporate the findings of these studies when developing packaging options that efficiently preserve food.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"21 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141698364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0