Biochimica et biophysica acta. General subjects最新文献

{"title":"Factories without walls: The molecular architecture and functions of non-membrane organelles in small RNA-guided genome protection","authors":"Shinichi Kawaguchi,&nbsp;Wakana Isshiki,&nbsp;Toshie Kai","doi":"10.1016/j.bbagen.2025.130811","DOIUrl":"10.1016/j.bbagen.2025.130811","url":null,"abstract":"<div><div>Non-membrane organelles, Yb body and nuage, play an essential role in piRNA-guided genome defense in <em>Drosophila</em> gonad by mediating piRNA biogenesis and transposon silencing. Yb body, found in somatic follicle cells, is responsible for primary piRNA processing, while nuage, located in germline cells, facilitates the ping-pong cycle to amplify the piRNAs corresponding to both sense and antisense strands of the expressed transposons. These organelles are assembled by liquid-liquid phase separation (LLPS) and protein-protein interactions, integrating RNA helicases (Vasa, Armitage), Tudor domain-containing proteins (Krimper, Tejas, Qin/Kumo), and proteins containing both domains (Yb, SoYb, Spn-E). Within these condensates, we summarize the protein-protein interactions experimentally validated and predicted by AlphaFold3, providing new structural insights into the non-membrane organelle assembly. This review highlights how the dynamic organization of Yb body and nuage enables efficient RNA processing, ensuring transposon suppression and genome stability.</div></div>","PeriodicalId":8800,"journal":{"name":"Biochimica et biophysica acta. General subjects","volume":"1869 7","pages":"Article 130811"},"PeriodicalIF":2.8,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemically administered mini α-crystallin peptide delays cataract progression in streptozotocin-induced diabetic rats","authors":"Pandarinath Savitikadi ,&nbsp;Lucky Dash ,&nbsp;Kiran Kumar Angadi,&nbsp;G. Bhanuprakash Reddy,&nbsp;V. Sudhakar Reddy","doi":"10.1016/j.bbagen.2025.130814","DOIUrl":"10.1016/j.bbagen.2025.130814","url":null,"abstract":"<div><div>α-Crystallin in the mammalian eye lens composed of αA-Crystallin (αAC) and αB-Crystallin (αBC) subunits present in a 3:1 ratio. These proteins exhibit chaperone-like activity, helping to protect cells from various forms of stress. Specific sequences within αAC (⁷⁰KFVIFLDVKHFSPEDLTVK⁸⁸) and αBC (⁷³DRFSVNLDVKHFSPEELKVK⁹²) have been shown to possess effective chaperone and anti-apoptotic properties. However, their protective effects in diabetic cataract (DC) have not been explored. The current study explored the protective effects of systemically administered mini-αA and αBC peptides, both individually and in combination (3:1 ratio) against streptozotocin (STZ)-induced DC in rats. Hyperglycemia was induced in Sprague-Dawley rats through intraperitoneal (I.P.) injection of STZ, while control rats received PBS. Starting from the onset of cataract development, a group of diabetic rats was treated with mini-αA, or mini-αB, or their combination for four months via IP administration. Cataract progression and maturation were monitored using a slit lamp biomicroscope. To understand the underlying biochemical and molecular processes, we assessed changes in protein content, protein insolubilization, oxidative stress, endoplasmic reticulum (ER) stress, apoptotic cell death, and caspase-3 activity. Although the mini peptides did not prevent STZ-induced hyperglycemia, they delayed cataract progression in diabetic rats. Furthermore, mini peptides reduced protein aggregation and insolubilization, alleviated oxidative and ER stress, and mitigated hyperglycemia-induced apoptosis by lowering caspase-3 activity and Bax levels. This study demonstrates that systemic administration of mini α-crystallin peptides can delay DC progression by mitigating protein aggregation, oxidative stress, ER stress, and apoptosis. These findings suggest potential therapeutic applications for mini α-crystallin peptides in treating DC.</div></div>","PeriodicalId":8800,"journal":{"name":"Biochimica et biophysica acta. General subjects","volume":"1869 7","pages":"Article 130814"},"PeriodicalIF":2.8,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"27-hydroxycholesterol impairs placental development via p53/p21/Cdk6 pathway: Implications for nutrient transport and cellular senescence","authors":"Zhaoyang Chen ,&nbsp;Xiaxia Cai ,&nbsp;Yuchen Wei ,&nbsp;Xiaoyan Zhao ,&nbsp;Qinyu Dang ,&nbsp;Yandi Zhu ,&nbsp;Ming Gao ,&nbsp;Yulu Zhang ,&nbsp;Yadi Zhang ,&nbsp;Huanling Yu","doi":"10.1016/j.bbagen.2025.130806","DOIUrl":"10.1016/j.bbagen.2025.130806","url":null,"abstract":"<div><div>Aberrant placental development and function contribute to various pregnancy complications. 27-hydroxycholesterol (27-OHC), a recognized mediator linking hypercholesterolemia and metabolic diseases, has an undefined role in placental development. This study investigates the impact of 27-OHC on placental development and its underlying mechanisms, particularly in relation to cellular senescence. Pregnant mice were subcutaneously administered either 27-OHC (27-OHC group) or normal saline (control group) during gestation. Subsequently, placentas underwent spatial transcriptome (ST) sequencing. The levels of genes and proteins related to nutrient transport, cell cycle and senescence associated secretory phenotype were validated. Additionally, BeWo cells were treated with 27-OHC at concentrations of 2.5, 5 and 10 μM during its differentiation and fusion to observe the effects and mechanisms of trophoblast cell senescence. In the 27-OHC group, the labyrinth zone area and combined fetal-placental weight were significantly reduced compared to the control group. ST analysis revealed alterations in placental cell composition and downregulation of nutrient transport processes, alongside pathways linked to senescence, including the p53/p21/Cdk6 pathway, specifically in Syncytiotrophoblast Type I (SynT I) cells. In both mouse placentas and BeWo cells, mRNA and protein levels of p53 and p21 were reduced in the 27-OHC group compared to controls. During late pregnancy, 27-OHC inhibits the physiological senescence of placental syncytiotrophoblasts and may affect nutrient transport within the placenta. The inhibition of the p53/p21/Cdk6 pathway may represent one of the key mechanisms involved.</div></div>","PeriodicalId":8800,"journal":{"name":"Biochimica et biophysica acta. General subjects","volume":"1869 7","pages":"Article 130806"},"PeriodicalIF":2.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probing the relationships between self-assembly and the antimicrobial activity of amyloidogenic peptides: The islet amyloid polypeptide as a case study","authors":"Vy Nguyen ,&nbsp;Mélanie Côté-Cyr ,&nbsp;Arthur Nery Finatto ,&nbsp;Margaryta Babych ,&nbsp;Phuong Trang Nguyen ,&nbsp;Mathew Sebastiao ,&nbsp;Steve Bourgault","doi":"10.1016/j.bbagen.2025.130812","DOIUrl":"10.1016/j.bbagen.2025.130812","url":null,"abstract":"<div><div>Antimicrobial peptides (AMPs) are key components of the innate immune system across diverse organisms. Interestingly, some AMPs can adopt β-sheet secondary structure and self-assemble into amyloid-like fibrils. Recent works have also revealed that amyloidogenic peptides exhibit antimicrobial properties and share a common mechanism of plasma membrane perturbation with AMPs. In this study, we explored the relationships between the antimicrobial activity of amyloidogenic peptides and their self-assembly by using the islet amyloid polypeptide (IAPP) as a model. IAPP is an aggregation-prone 37-residue hormone whose pancreatic deposition and accumulation are associated with type II diabetes. Antimicrobial assays revealed that IAPP monomers and prefibrillar aggregates, including soluble oligomers, inhibit the growth of <em>Escherichia coli</em> and <em>Staphylococcus epidermidis</em>. Additionally, monomeric and prefibrillar proteospecies perturbed anionic lipid vesicles that mimic bacterial plasma membrane and decrease the metabolic activity. In contrast, pre-assembled amyloid fibrils exhibited weak antimicrobial activities and lipid membrane perturbation, although they agglutinated bacteria avidly. By taking advantage of residue-specific substitutions that modulate the aggregation propensity, we observed that derivatives with hindered amyloidogenicity retained antimicrobial activities, while those with accelerated kinetics of amyloid self-assembly had weaker antimicrobial effect. Moreover, by modulating the propensity of IAPP to fold into an α-helix, we observed that amyloid formation is not a prerequisite for the antimicrobial activity, while the destabilization of helical folding reduced IAPP antimicrobial activity. This study provides fundamental mechanistic insights of the modest antimicrobial activity of IAPP and highlights that precaution should be taken before generalizing the antimicrobial potential of self-assembling amyloid polypeptides.</div></div>","PeriodicalId":8800,"journal":{"name":"Biochimica et biophysica acta. General subjects","volume":"1869 7","pages":"Article 130812"},"PeriodicalIF":2.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"G-quadruplex in cancer energy metabolism: A potential therapeutic target","authors":"Zongqiang Han ,&nbsp;Lina Wen","doi":"10.1016/j.bbagen.2025.130810","DOIUrl":"10.1016/j.bbagen.2025.130810","url":null,"abstract":"<div><div>In recent years, energy metabolism in cancer has received increasing attention as an important component of tumor biology, and the functions of transcription factors, mitochondria, reactive oxygen species (ROS) and the autophagy-lysosome system in which have been elucidated. G-quadruplex (G4) is a molecular switch that regulates gene transcription or translation. As an anticancer target, the effect of G4 on cancer cell proliferation, apoptosis, cycle and autophagy has been recognized. The energy metabolism system is a unified whole composed of transcription factors, metabolic regulators, metabolites and signaling pathways that run through the entire cancer process. However, the role of G4 in this complex metabolic network has not been systematically elucidated. In this review, we analyze the close correlation between G4 and transcription factors, mitochondria, ROS and the autophagy-lysosome system and suggest that G4 can exert a marked effect on cancer energy metabolism by regulating the above mentioned key regulatory elements. The anticancer effects of some G4 ligands through regulation of energy metabolism have also been summarized, confirming the clear involvement of G4 in energy metabolism. Although much more research is needed, we propose that G4 may play a critical role in the complex energy metabolism system of cancer, which is a promising target for anticancer strategies focusing on energy metabolism.</div></div>","PeriodicalId":8800,"journal":{"name":"Biochimica et biophysica acta. General subjects","volume":"1869 7","pages":"Article 130810"},"PeriodicalIF":2.8,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pipecolic acid: A positive regulator of systemic acquired resistance and plant immunity","authors":"Megha Kumari ,&nbsp;Prashansa Sharma ,&nbsp;Archana Singh","doi":"10.1016/j.bbagen.2025.130808","DOIUrl":"10.1016/j.bbagen.2025.130808","url":null,"abstract":"<div><div>Pipecolic acid (Pip) is a naturally occurring non-protein amino acid, that builds up in plants in response to pathogen infection. Pip is upregulated in autophagy mutants, indicating its role as a crucial regulator of plant immunity by upregulating systemic acquired resistance (SAR). This broad-spectrum defense mechanism protects uninfected parts of the plant during subsequent pathogen attacks. Pip has been identified as a SAR chemical signal and acts before the NO-ROS-AzA-G3P. The biosynthesis of Pip begins with lysine by the activity of ALD1 and SARD4 in a sequential manner; ALD1, a lysine aminotransferase, catabolizes lysine to Δ 1-piperidine-2-carboxylic acid, which is further modified to Pip by the activity of ornithine cyclodeaminase activity of SARD4. Additionally, FMO 1, a flavin monooxygenase, catalyzes the synthesis of N-hydroxy-pipecolic acid (NHP, the final, SAR-inducing defense hormone) from Pip. Pip and its active form accumulate at the infection site in the phloem and are transported to distal parts of the plant via symplast to trigger SAR. This review focuses on the roles of Pip and NHP in regulating SAR and how they interact with other defense signals like salicylic acid (SA) to modulate plant immunity.</div></div>","PeriodicalId":8800,"journal":{"name":"Biochimica et biophysica acta. General subjects","volume":"1869 7","pages":"Article 130808"},"PeriodicalIF":2.8,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Celebrating 50 years of fluorescence correlation spectroscopy (FCS): Advancing live-cell massively parallel FCS studies with photostable GFPs, mStayGold and StayGold/E138D","authors":"Sho Oasa ,&nbsp;Borislav Stoyanov ,&nbsp;Yuta Hamada ,&nbsp;Stanko N. Nikolić ,&nbsp;Aleksandar J. Krmpot ,&nbsp;Akira Kitamura ,&nbsp;Vladana Vukojević","doi":"10.1016/j.bbagen.2025.130809","DOIUrl":"10.1016/j.bbagen.2025.130809","url":null,"abstract":"<div><div>More than 50 years after its inception, fluorescence correlation spectroscopy (FCS) remains a cornerstone technique for quantitative characterization of the cellular dynamics of molecules and their concentration and interactions in live cells. The enhanced green fluorescent protein (eGFP) has long been a preferred tag in live-cell FCS, valued for its brightness, photostability and lack of posttranslational modifications. However, low eGFP photostability limits measurement durations, posing challenges for studying dynamic cellular processes necessitating longer measurement time. Recent advancements in fluorescent protein engineering have yielded mStayGold and StayGold/E138D, two highly photostable monomeric GFP variants. In this study, we evaluate their performance in live cells and utility for FCS by quantifying glucocorticoid receptor (GR) homodimerization and nuclear import/export dynamics in live cells. Our study shows that both mStayGold and StayGold/E138D exhibit twice the brightness of eGFP, significantly enhancing the signal-to-noise ratio (SNR). Using massively parallel FCS (mpFCS) and two-foci cross-correlation to characterize the direction of GR nucleocytoplasmic transport along the nuclear envelope, we also confirm that these proteins show significantly improved photostability over eGFP.</div></div>","PeriodicalId":8800,"journal":{"name":"Biochimica et biophysica acta. General subjects","volume":"1869 7","pages":"Article 130809"},"PeriodicalIF":2.8,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aryl hydrocarbon receptor (AHR) signalling: A double-edged sword guiding both cancer progression and cancer therapy","authors":"Rahul Sahoo ,&nbsp;Sriya Pattnaik ,&nbsp;Biswajit Mohanty ,&nbsp;Showkat Ahmad Mir ,&nbsp;Birendra Behera","doi":"10.1016/j.bbagen.2025.130805","DOIUrl":"10.1016/j.bbagen.2025.130805","url":null,"abstract":"<div><div>Aryl Hydrocarbon Receptor (AHR) reported to be associated with major carcinogenic signalling cascades which cause cell proliferations, metastasis and invasion as well as immune imbalance. AHR Participates in cellular processes not only through genomic pathways to cause genomic alterations but also via nongenomic pathways to alter various cytoplasmic proteins. In addition, AHR senses a wide range of ligands that modulate its downstream mechanisms that are intricated in cancer induction and prevention. Thus, AHR functions as a two-sided sword where some AHR ligands contribute to enhance cancer whereas few are useful for cancer treatment. Therefore, AHR represent as a regulatory point in cancer progression and treatment. There is a need to reinvestigate the regulatory role of AHR in major intracellular pathways and to explore the potential of AHR ligand for the design of cancer therapeutics. This review emphasizes the interaction of AHR with pro-carcinogenic signalling pathways that modulate cancer induction and progression. Furthermore, it also discusses about the current discovery of AHR ligands for cancer initiation or inhibition. This information could be useful for development of therapeutic strategies for the management of cancer by targeting AHR.</div></div>","PeriodicalId":8800,"journal":{"name":"Biochimica et biophysica acta. General subjects","volume":"1869 6","pages":"Article 130805"},"PeriodicalIF":2.8,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harmonizing time with survival: Circadian rhythm and autophagy in plants","authors":"Laha Supriya ,&nbsp;Deepika Dake ,&nbsp;Mehanathan Muthamilarasan","doi":"10.1016/j.bbagen.2025.130807","DOIUrl":"10.1016/j.bbagen.2025.130807","url":null,"abstract":"<div><h3>Background</h3><div>Circadian rhythm (CR) is a self-sustaining biological oscillation that synchronizes physiological processes with the Earth's 24-h light-dark cycle. In plants, it regulates crucial physiological functions. Autophagy, a conserved degradation mechanism, maintains cellular homeostasis by recycling damaged organelles and proteins. Emerging evidence suggests an interplay between CRs and autophagy, optimizing plant survival and productivity.</div></div><div><h3>Scope</h3><div>This review explores the molecular mechanisms underlying CR and autophagy, highlighting their roles in growth and stress adaptation. It further examines how circadian clock components regulate autophagy-related genes (ATGs) in response to external cues.</div></div><div><h3>Major conclusions</h3><div>CR fine-tune autophagy by temporally regulating ATG gene expression. Key transcription factors, including TOC1 and LUX, modulate autophagic activity, ensuring energy conservation. Autophagy reciprocally influences circadian signaling, adjusting metabolic balance under stress.</div></div><div><h3>General significance</h3><div>Despite extensive research on circadian regulation, a comprehensive understanding of how core clock components orchestrate ATG gene expression remains lacking. Understanding the crosstalk between CR and autophagy provides insights into plant resilience and productivity, potentially informing crop improvement strategies that enhance stress tolerance and resource efficiency. This review aims to bridge this gap by summarizing recent insights and proposing future research directions.</div></div>","PeriodicalId":8800,"journal":{"name":"Biochimica et biophysica acta. General subjects","volume":"1869 6","pages":"Article 130807"},"PeriodicalIF":2.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redox modulation via a synthetic thiol compound reshapes energy metabolism in endothelial cells and ameliorates angiogenic expression in a co-culture study with activated macrophages","authors":"Michela Bruschi,&nbsp;Sofia Masini,&nbsp;Federica Biancucci,&nbsp;Giovanni Piersanti,&nbsp;Barbara Canonico,&nbsp;Michele Menotta,&nbsp;Mauro Magnani,&nbsp;Alessandra Fraternale","doi":"10.1016/j.bbagen.2025.130803","DOIUrl":"10.1016/j.bbagen.2025.130803","url":null,"abstract":"<div><div>The vascular endothelium is the first interface exposed to circulating compounds and oxidative as well as pro-inflammatory <em>stimuli</em>. Nowadays, cysteine pro-drugs are emerging as new and potential therapies in cardiovascular and inflammatory diseases due to their cytoprotective effects. In this study, the effects of redox modulation by a synthetic thiol compound, i.e., I-152, a precursor of <em>N</em>-acetylcysteine (NAC) and cysteamine (MEA), were evaluated after 6 h and 24 h treatment on human umbilical cord endothelial cell (HUVECs) energy metabolism. Following I-152 treatment, higher cysteine and glutathione (GSH) content were detected via HPLC, in concomitance with I-152 derivatives, i.e., NAC and MEA. Untargeted metabolomics confirmed GSH upregulation and NAC presence in addition to I-152 itself and other metabolites, such as dithiol compound (NACMEAA) and triacetylated I-152. Mass spectrometry revealed that I-152 boosted ATP production, specifically through the mitochondrial OXPHOS, as determined via Seahorse assay without inducing oxidative stress. Additionally, I-152 treatment of HUVECs before co-culture with LPS-stimulated macrophages provided GSH and cysteine sustainment and attenuated the transcription of adhesion molecules as well as <em>iNOS</em> expression. Identifying the impact of redox regulation in physiological conditions and the possible metabolic targets could aid the application of novel thiol-based therapeutics.</div></div>","PeriodicalId":8800,"journal":{"name":"Biochimica et biophysica acta. General subjects","volume":"1869 6","pages":"Article 130803"},"PeriodicalIF":2.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143786054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}