Drought stress induces variation in DNA methylation pattern in a genotype-dependent manner in chickpea.

IF 2.8 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Khushboo Gupta, Rohini Garg
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引用次数: 0

Abstract

When plants are exposed to harsh environmental conditions, such as extreme temperatures or drought, certain genes are turned on or off. This process can be controlled by a chemical modification to their DNA called methylation. Here, we examined the impact of DNA methylation during drought stress on two chickpea genotypes, ICC 1882 (drought sensitive, DS) and ICC 4958 (drought tolerant, DT) chickpea genotypes via whole-genome bisulfite sequencing. A higher degree of hypomethylation in the DT genotype and more hypermethylation in the DS genotype were observed. A positive correlation was observed between CG methylation with genes and CHH methylation with TEs. Functional annotation of differentially methylated regions associated with differentially expressed genes revealed distinct pathways enriched in DS, such as enrichment of genes involved in root development, telomere maintenance, ion transport, and regulation of gene expression, while pathways like apoptosis, silencing by miRNAs, programmed cell death and carotenoid metabolic processes were enriched in DT genotype. Further, small RNA distribution and non-CWA context methylation density in TEs suggested the role of the RdDM pathway in mediating CHH hypermethylation in transposable elements. Overall, we observed distinct genes are differentially expressed and differentially methylated under drought stress in sensitive and tolerant genotypes.

干旱胁迫诱导鹰嘴豆DNA甲基化模式以基因型依赖的方式发生变化。
当植物暴露在恶劣的环境条件下,比如极端温度或干旱,某些基因就会开启或关闭。这个过程可以通过一种叫做甲基化的化学修饰来控制。通过亚硫酸盐全基因组测序,研究了干旱胁迫下DNA甲基化对两种鹰嘴豆基因型ICC 1882(干旱敏感型,DS)和ICC 4958(干旱耐旱型,DT)的影响。DT基因型的低甲基化程度较高,DS基因型的高甲基化程度较高。CG甲基化与基因呈正相关,CHH甲基化与TEs呈正相关。对与差异表达基因相关的差异甲基化区域的功能注释显示,DS中富集了不同的途径,如参与根发育、端粒维持、离子运输和基因表达调控的基因,而DT基因型中富集了凋亡、mirna沉默、细胞程序性死亡和类胡萝卜素代谢过程等途径。此外,te中较小的RNA分布和非cwa上下文甲基化密度表明RdDM途径在介导转座元件CHH超甲基化中的作用。总的来说,我们观察到在干旱胁迫下,敏感型和耐受性基因型中不同基因的差异表达和差异甲基化。
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来源期刊
Biochimica et biophysica acta. General subjects
Biochimica et biophysica acta. General subjects 生物-生化与分子生物学
CiteScore
6.40
自引率
0.00%
发文量
139
审稿时长
30 days
期刊介绍: BBA General Subjects accepts for submission either original, hypothesis-driven studies or reviews covering subjects in biochemistry and biophysics that are considered to have general interest for a wide audience. Manuscripts with interdisciplinary approaches are especially encouraged.
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