Biocontrol science最新文献_第2页

Theoretical Base for the Application of the DiVSaL Method to Bacterial Spores to Evaluate Injured Populations Occurring After Exposure to Lethal Stress. 应用DiVSaL方法评价致死应激后细菌孢子损伤群体的理论基础。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2022-01-01 DOI: 10.4265/bio.27.169

Ryoko Asada, Jin J Sakamoto, Masakazu Furuta, Tetsuaki Tsuchido

{"title":"Theoretical Base for the Application of the DiVSaL Method to Bacterial Spores to Evaluate Injured Populations Occurring After Exposure to Lethal Stress.","authors":"Ryoko Asada, Jin J Sakamoto, Masakazu Furuta, Tetsuaki Tsuchido","doi":"10.4265/bio.27.169","DOIUrl":"https://doi.org/10.4265/bio.27.169","url":null,"abstract":"To assess injury in bacterial spore populations exposed to lethal stress, we proposed a theoretical basis for applying the DiVSaL method, which has already been reported for general microorganisms as a double subculture method. We constructed a mathematical model in which both injuries to the germination system and the spore body were taken into the theory. In this theory, we reasonably assumed that the viable and germinable spore count is constant before the subsequent vegetative growth and that the delay of germination and outgrowth can be included in the concept of λ injury previously reported as the growth-independent injury. By introducing these assumptions, the double subculture method can be considered to apply to spores as well. As examples of the application of this theory, the growth delays of Bacillus subtilis spores treated with heat and UV irradiation were analyzed and the numbers of injured spores were evaluated. Based on the results obtained, heat is indicated to have a higher injury generation ability than UV irradiation. The applicability of the DiVSaL method as a tool for food preservation and sanitation designs is presented.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"27 3","pages":"169-177"},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33500467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mechanism of Polyhexamethylene Biguanide Resistance in Purpureocillium lilacinum Strains. 紫丁香紫霉耐聚己二烯双胍的机制。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2022-01-01 DOI: 10.4265/bio.27.117

Yikelamu Alimu, Yoko Kusuya, Takako Yamamoto, Kana Arita, Naofumi Shigemune, Hiroki Takahashi, Takashi Yaguchi

{"title":"Mechanism of Polyhexamethylene Biguanide Resistance in Purpureocillium lilacinum Strains.","authors":"Yikelamu Alimu, Yoko Kusuya, Takako Yamamoto, Kana Arita, Naofumi Shigemune, Hiroki Takahashi, Takashi Yaguchi","doi":"10.4265/bio.27.117","DOIUrl":"https://doi.org/10.4265/bio.27.117","url":null,"abstract":"Purpureocillium lilacinum has been recently found to contaminate a 20% (200,000 μg/mL) aqueous solution of polyhexamethylene biguanide hydrochloride (PHMB) . We aimed to elucidate the mechanism underlying the resistance of P. lilacinum to PHMB. First, we induced the PHMB-resistant (IR) strains IFM 67050 (IR) and IFM 65838 (IR) from the type strain P. lilacinum CBS 284.36T via cultivation in a medium containing high concentrations of PHMB. We then analyzed the DNA sequences via Illumina sequencing to evaluate the presence of genetic mutations in IFM 65838 (IR) . Further, we established an IFM 65838 (IR) uridine/uracil auxotrophic strain, and using the orotidine-5'-decarboxylase gene, pyrG as a selection marker, we tried to knockout a mutant gene in IFM 65838 (IR) using the CRISPR-Cas9 genome-editing technique. The growth rates of IFM 67050 (IR) and IFM 65838 (IR) in medium containing PHMB increased, and the minimum inhibitory concentrations (MICs) against PHMB also increased. Based on the DNA sequence analysis, we found a nonsynonymous point mutation in the gene PLI-008146 (G779A) in IFM 67050 (IR) and IFM 65838 (IR) . This point mutation leads to site combinations of splicing changes that cause partial sequences deletion (p.Y251_G281del) in the ΔPLI-008146 locus of IFM 65838 (IR) , and deletion sequences include partial adenosine/AMP deaminase motif (PF00962) orthologous to adenosine deaminase (ADA) (GeneBank: OAQ82383.1) . Furthermore, the mutant gene ΔPLI-008146 was successfully knocked out from the resistanceinduced strain using a novel CRISPR-Cas9 gene transformation method. A considerable reduction in growth rate and MIC against PHMB was observed in the absence of the mutant gene. Therefore, ADA may represent an important resistance factor in PHMB-resistant P. lilacinum.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"27 3","pages":"117-130"},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33499488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Remote Bactericidal Effect of Anatase TiO₂ Photocatalytic Nanoparticles Annealed with Low-Temperature O₂ Plasma. 低温O2等离子体退火锐钛矿型TiO2光催化纳米颗粒的远程杀菌效果

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2022-01-01 DOI: 10.4265/bio.27.217

Retsuo Kawakami, Yuki Takao, Akihiro Shirai, Takashi Mukai

{"title":"Remote Bactericidal Effect of Anatase TiO2 Photocatalytic Nanoparticles Annealed with Low-Temperature O2 Plasma.","authors":"Retsuo Kawakami, Yuki Takao, Akihiro Shirai, Takashi Mukai","doi":"10.4265/bio.27.217","DOIUrl":"https://doi.org/10.4265/bio.27.217","url":null,"abstract":"The remote bactericidal effect of TiO2 photocatalyst, i.e., the bactericidal effect away from the photocatalyst, was successfully achieved using a humidified airflow. The TiO2 photocatalyst used was anatase-type TiO2 nanoparticles (NPs) annealed with a low-temperature O2 plasma. For comparison, anatase-type TiO2 NPs annealed in the air were used. The bacteria, Bacillus subtilis, were placed away from the TiO2 NPs. The plasma-assisted-annealed TiO2 NPs significantly inactivated 99% of the bacterial cells in 5 h, whereas the pristine and air-annealed TiO2 NPs inactivated 88-90% of the bacterial cells. The remote bactericidal effect of plasmaassisted-annealed TiO2 NPs would be attributed to a larger amount of H2O2 molecules traveled by the airflow from the TiO2 NPs. The molecules were generated by chemically reacting more photoexcited carriers on the TiO2 surface with H2O and O2 in the airflow. These photoexcited carriers originated from more oxygen-based species adsorbed and more oxygen vacancies introduced on the TiO2 surface by the plasma-assisted-annealing.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"27 4","pages":"217-222"},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10435325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Violet-Blue Light-Emitting Diode on Controlling Bacterial Contamination in Boiled Young Sardine. 紫-蓝发光二极管对水煮沙丁鱼幼鱼细菌污染的控制效果。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2022-01-01 DOI: 10.4265/bio.27.9

Akihiro Shirai, Yu-Ko Yasutomo, Yuka Kanno

{"title":"Effects of Violet-Blue Light-Emitting Diode on Controlling Bacterial Contamination in Boiled Young Sardine.","authors":"Akihiro Shirai, Yu-Ko Yasutomo, Yuka Kanno","doi":"10.4265/bio.27.9","DOIUrl":"https://doi.org/10.4265/bio.27.9","url":null,"abstract":"The aim of this study was to evaluate bacterial decontamination of boiled young sardine by treatment with violet-blue light followed by cooling storage of the irradiated boiled sardine. Viable cell count in the samples was evaluated after irradiation with four types of violet-blue light-emitting diodes (LEDs; peak wavelength at 405, 412, 421 or 455 nm) and subsequent cooling storage for two days. LED (405 nm) exhibited bactericidal and growth suppression effects. The irradiation gave a 47% bactericidal rate in comparison with no irradiation samples (control) and the two-day storage suppressed the increase in cell counts to 24%, while the rate of increase was 545% for the control. Integrated viability (IV) based on growth delay analysis was estimated after irradiation of four isolates from boiled sardine with 405 nm light. The irradiation caused growth delay against all isolates, resulting in smaller IV values for three isolates compared to those viabilities estimated from colony forming units. Exposure (405 nm) at 432 J/cm2 fluence resulted in a decrease in water content, resulting in an increase in salinity of the samples. This study demonstrated the advantages of light emitting a narrow violet region as a non-thermal disinfection technology in the processing and storage of boiled sardines.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"27 1","pages":"9-19"},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40311189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of Serotyping of Environmental and Clinical Isolates of Legionella pneumophila using MALDI-TOF MS. 用MALDI-TOF质谱评价嗜肺军团菌环境和临床分离株血清分型。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2022-01-01 DOI: 10.4265/bio.27.81

Kazuyuki Sogawa, Naoto Ishizaki, Takayuki Ishige, Syota Murata, Toshibumi Taniguchi, Katsunori Furuhata

{"title":"Evaluation of Serotyping of Environmental and Clinical Isolates of Legionella pneumophila using MALDI-TOF MS.","authors":"Kazuyuki Sogawa, Naoto Ishizaki, Takayuki Ishige, Syota Murata, Toshibumi Taniguchi, Katsunori Furuhata","doi":"10.4265/bio.27.81","DOIUrl":"https://doi.org/10.4265/bio.27.81","url":null,"abstract":"Legionella pneumophila (L. pneumophila) is responsible for most Legionnaire's disease cases diagnosed worldwide. The species includes 16 serogroups, but most Legionnaire's disease cases (85.7% in Europe, 87.0% in Japan) are caused by L. pneumophila serogroup 1. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be used to identify the L. pneumophila serogroup. In this study, we compared three sample preparation methods that are compatible with MALDI-TOF MS: the direct colony transfer method (DCTM), on-target extraction method (OTEM), and in-tube extraction method (ITEM). The aim was to improve the low identification rates for L. pneumophila, and establish and validate a simple, rapid and robust MALDI-TOF MS-based method for routine use in microbiological laboratories for assignment of L. pneumophila isolates to serogroups and identification of reliable peak biomarkers. Using ITEM, 100.0% (29/29) of hot spring water samples and clinical isolates were correctly identified at the species level. Augmented reference spectra correctly identified all 29 strains at the species level and 29 isolates at the serogroup level, displaying sensitivity, specificity and accuracy of 100.0% for serogroup assignment. MALDI-TOF MS is a relatively inexpensive method for assignment of L. pneumophila serogroups that can serve as a first-line tool for rapid prospective typing.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"27 2","pages":"81-86"},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40399305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Low Temperature Heating-Induced Death and Vacuole Injury in Cladosporium sphaerospermum Conidia. 低温加热致球孢子囊孢子死亡和液泡损伤。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2022-01-01 DOI: 10.4265/bio.27.107

Shigetoshi Horikiri, Mami Harada, Ryoko Asada, Jin J Sakamoto, Masakazu Furuta, Tetsuaki Tsuchido

{"title":"Low Temperature Heating-Induced Death and Vacuole Injury in Cladosporium sphaerospermum Conidia.","authors":"Shigetoshi Horikiri, Mami Harada, Ryoko Asada, Jin J Sakamoto, Masakazu Furuta, Tetsuaki Tsuchido","doi":"10.4265/bio.27.107","DOIUrl":"https://doi.org/10.4265/bio.27.107","url":null,"abstract":"The mechanism of thermal death of mold conidia has not been understood in detail. The purpose of this study is to analyze the death kinetics of heated conidia of Cladosporium sphaerospermum and to ascertain the expectant cell injury responsible for the death. The death of the dormant (resting) conidia of Cladosporium sphaerospermum was examined at temperatures of between 43 and 54℃ with the conventional colony count method. The death reaction apparently followed the first order kinetics, but the Arrhenius plot of the death rate constant demonstrated seemingly a break. The linearity at temperatures higher than that at the break was lost at lower temperatures, suggesting the involvement of an unusual mechanism in the latter temperatures. In the cell morphology, we observed with quinacrine staining the vacuole rupture at a lower temperature but not at a high temperature. Interestingly, the vacuole rupture by low-temperature heating was found to correlate with the viability loss. Furthermore, active protease originally locating in vacuoles was detected in the cytoplasm of the conidia after heated at a low temperature. The results obtained suggest the involvement of potent autophagic cell death induced by low temperature heating of C. sphaerospermum conidia.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"27 2","pages":"107-115"},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40400202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Species Diversity based on Revised Systematics of Xerophilic Aspergillus section Restricti Isolated from Storage Rooms and Houses in Japan. 基于日本仓库和房屋分离的干燥曲霉切片修订系统分类的物种多样性。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2022-01-01 DOI: 10.4265/bio.27.65

Ryo Hagiuda, Hisayuki Oda, Yuji Kawakami, Dai Hirose

{"title":"Species Diversity based on Revised Systematics of Xerophilic Aspergillus section Restricti Isolated from Storage Rooms and Houses in Japan.","authors":"Ryo Hagiuda, Hisayuki Oda, Yuji Kawakami, Dai Hirose","doi":"10.4265/bio.27.65","DOIUrl":"https://doi.org/10.4265/bio.27.65","url":null,"abstract":"Eighty-seven strains of Aspergillus section Restricti were isolated from five storage rooms (50 strains) and 21 houses (37 strains) between 2014 and 2020. Eleven species were identified based on their morphological characteristics and molecular phylogeny using the rRNA internal transcribed spacer (ITS) region, calmodulin (CaM), β-tubulin (benA), and RNA polymerase II second largest subunit (RPB2) sequences. A. penicillioides, which was known to cause the deterioration of cultural assets, was isolated at high frequency (73%) from the surfaces of 11 cultural assets in the storage rooms; A. clavatophorus and A. magnivesiculatus, which are closely related to A. penicillioides, were also isolated frequently (45 and 64%, respectively). Five species [A. clavatophorus (42.8%), A. penicillioides (42.8%), A. magnivesiculatus (14.3%), A. reticulatus (28.6%), and A. vitricola (28.6%)] were isolated from dust on the carpets in seven houses. Five species [A. clavatophorus (33.3%), A. penicillioides (55.5%), A. magnivesiculatus (44.4%), A. restrictus (44.4%), and A. gracilis (11.1%)] were isolated from dust on the bedding in nine houses. Using the taxonomic system described by Sklenář et al. (2017), five species (A. clavatophorus, A. magnivesiculatus, A. hordei, A. reticulatus, and A. glabripes) previously identified as A. penicillioides were confirmed as new to Japan.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"27 2","pages":"65-80"},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40400204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of the Cleanliness of Spray Nozzle on the Concentration of Microorganisms in the Spray Water in Warm-Water Bidet Toilet Seats. 喷头清洁度对温水坐浴盆座圈喷雾水中微生物浓度的影响

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2022-01-01 DOI: 10.4265/bio.27.153

Toru Iyo, Keiko Asakura, Kazuyuki Omae

引用次数: 0

Examination of Conditions for External Quality Control in Identification of Microorganisms using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. 基质辅助激光解吸/电离飞行时间质谱法鉴定微生物外部质量控制条件的研究。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2022-01-01 DOI: 10.4265/bio.27.179

Kazuyuki Sogawa, Kohei Uechi, Makiko Kiyosuke, Syota Murata, Koji Kusaba, Yuji Saeki, Kazuki Horiuchi, Kazunari Yasuda, Hajime Okumura, Azumi Fujinaga, Masami Murakami

引用次数: 0

The Effectiveness of Neutral Electrolyzed Water for Decontaminating the Spray Nozzles of Electric Tankless and Tank-Type Warm-Water Bidet Toilet Seats. 中性电解水对电即热式和罐式温水坐浴盆坐便器喷头去污效果的研究。

IF 1.2 4区农林科学

Biocontrol science Pub Date : 2022-01-01 DOI: 10.4265/bio.27.193

Toru Iyo, Keisuke Iwahori

{"title":"The Effectiveness of Neutral Electrolyzed Water for Decontaminating the Spray Nozzles of Electric Tankless and Tank-Type Warm-Water Bidet Toilet Seats.","authors":"Toru Iyo, Keisuke Iwahori","doi":"10.4265/bio.27.193","DOIUrl":"https://doi.org/10.4265/bio.27.193","url":null,"abstract":"Electrolyzed water is a novel disinfectant that is widely used in the food industry. We conducted an experimental model-based study to determine the effectiveness of neutral electrolyzed water (NEW) for the daily nozzle cleaning of artificially contaminated tankless and tank-type bidet toilet seats. The toilet seats were designed to automatically self-clean the spray nozzles using tap water or NEW after each use or at specified intervals. The numbers of Pseudomonas aeruginosa and Escherichia coli microorganisms in the spray water were measured twice or thrice per week. A Kruskal-Wallis test was used to compare the bacterial count in the spray water of various cleaning (NEW) and control (tap water) conditions. The number of bacteria was significantly lower in NEW conditions with and without periodic nozzle cleaning functions than in tap water conditions for both tank-type and tankless bidet toilet seats. Microorganisms were detected only on the surface area around the opening for ejecting spray water and not in the internal piping at the spray nozzle tip. These findings demonstrate that NEW has superior decontamination efficacy over tap water when used as a cleaning agent for the spray nozzles of warm-water bidet toilet seats.","PeriodicalId":8777,"journal":{"name":"Biocontrol science","volume":"27 4","pages":"193-200"},"PeriodicalIF":1.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10435328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0