Berliner und Munchener tierarztliche Wochenschrift最新文献

{"title":"Within-pool prevalence limits for the identification of paratuberculosis infected herds using antibody detection in pooled milk samples","authors":"H. Köhler, M. Ziller, F. Gierke, K. Donat","doi":"10.2376/0005-9366-16019","DOIUrl":"https://doi.org/10.2376/0005-9366-16019","url":null,"abstract":"Identification of Mycobacterium avium subsp. paratuberculosis (MAP) infected herds is the first step in paratuberculosis control. Bulk milk or pooled milk sampling is a widely used, cost-effective approach for the surveillance of infectious diseases in dairy cattle. Due to insufficient data about the diagnostic performance, it has rarely been exploited for paratuberculosis. Using a logistic regression model, threshold levels of the apparent within-pool prevalence (WPPapp) were determined for the identification of paratuberculosis positive herds by antibody detection in pools of 50 individual milk samples, which is a usual pool size. A total of 73 milk pools were prepared, 50 from two MAP-positive and 23 from one MAP-non-suspect herds. Four different commercial ELISAs were used. WPPapp was estimated independently based on antibody positive individual milk or serum samples and on positive faecal culture. Antibody detection resulted in lower WPPapp estimates than faecal culture (FWPPapp) and tended to underestimate true prevalence. For pooled milk testing, cut-off values of the four ELISAs were revised to ensure 99% specificity and high sensitivity. For 50% probability of detection, FWPPapp thresholds of 8.9–16.3% were determined, increasing to 20.0–37.8% for 95% probability of detection. The results underline that antibody detection in pools of 50 individual milk samples or bulk tank milk from herds ≤ 50 cows allows only the identification of herds with a very high prevalence of MAP-shedders. In control programs this can be the first step to identify the most affected herds. However, it is ineffective for prevalence investigations, surveillance programs and certification.","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"51 1","pages":"34-41"},"PeriodicalIF":0.4,"publicationDate":"2017-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87368792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unterschiede in der Berechnung des betriebsbezogenen Antibiotika-Einsatzes in Monitoringsystemen in Deutschland – eine Übersicht","authors":"Malin Hemme, A. Käsbohrer, C. Münchhausen, M. Hartmann, R. Merle, L. Kreienbrock","doi":"10.2376/0005-9366-16065","DOIUrl":"https://doi.org/10.2376/0005-9366-16065","url":null,"abstract":"In Deutschland wird die Abgabe bzw. der Verbrauch von Antibiotika in der Nutztierhaltung kontinuierlich erfasst. Hierzu dienen unter anderem die nach Arzneimittelgesetz verpflichtende Dokumentation in der behordlichen HI-Tier Datenbank, die Dokumentation innerhalb des privatwirtschaftlichen Systems der Firma QS Qualitat und Sicherheit GmbH aber auch wissenschaftliche Untersuchungen wie das Projekt VetCAb („Veterinary Consumption of Antibiotics“) oder andere bestandsspezifische Untersuchungen. Diese Systeme berechnen die Haufigkeit des Antibiotikaeinsatzes uber die Therapiehaufigkeit oder aquivalente Maszahlen, die jedoch in jedem System unterschiedlich definiert werden. Da haufig keine detaillierte Beschreibung der genutzten Maszahl angefuhrt wird, kommt es bei der Interpretation immer wieder zu Missverstandnissen. Um eine Ubersicht uber die verwendeten Begriffe zu erhalten, werden vier der in Deutschland angewendeten Maszahlen und die dazugehorigen Grosen beschrieben: der Tierbehandlungsindex, der Therapieindex nach Definition der Fa. QS Qualitat und Sicherheit GmbH, die Therapiehaufigkeit nach Festlegung des Arzneimittelgesetzes sowie die Therapiehaufigkeit, die im Rahmen des Projektes VetCAb zur Anwendung kommt. Durch den Vergleich der Definitionen der Maszahlen sowie einzelner genutzter Variablen wird deutlich, dass in den errechneten Zielgrosen (nummerische) Unterschiede bestehen konnen und somit ein direkter Vergleich der vier Maszahlen nicht statthaft ist. Alle Maszahlen sind jedoch innerhalb des jeweiligen Systems geeignet, Trends zu analysieren und zu vergleichen.","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"32 1","pages":"93-101"},"PeriodicalIF":0.4,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85937633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erfassung der Klauengesundheit in Anbinde- und Laufställen von bayerischen Fleckviehkuhherden und deren Auswirkung auf die Milchleistung","authors":"E. Zeiler, M. Hanemann, Hubert Reszler, J. Duda, D. Sprengel, K.-U. Götz, G. Knubben-Schweizer, C. Sauter-Louis","doi":"10.2376/0005-9366-16038","DOIUrl":"https://doi.org/10.2376/0005-9366-16038","url":null,"abstract":"The aim of the present study was to examine the effects of the housing systems loose house (LH) and tie stall (TS) on claw health and the effect on the milk production for dairy cows of the breed Fleckvieh. 35 Bavarian farms participated in the study, comprising 1435 Fleckvieh cows in total. 100 -day performance was observed by 333 cows, the 305 -day performance in 289 cows. The evaluation of claw health was part of the regular claw care. Documentation was done according to the commonly applied diagnosis code of the German Agricultural Society (Deutsche Landwirtschafts Gesellschaft, DLG). The diagnosed claw health conditions were graded according to the DLG-scheme: grade 1 (low), grade 2 (medium) and grade 3 (severe), whereby the proportion of cows in grade 3 was very low (about 6%). Milk production records were provided from routine performance recording. Statistical analysis was performed using multivariate analysis of variance. The percentage of affected animals kept in loose houses was significantly higher than the one of the animals kept in tie stalls (LH 79.3%, TS 62.6%). In both farming systems white-line-disease (LH 40.3%, TS 33.2%) and laminitis (LH 34.7%, TS 26.8%) were most prevalent. Milk production of LH-animals was significantly affected by claw health status. A significantly lower 100 -day performance was observed in animals with moderate to severe claw disorders compared to healthy animals (around 480 kg milk/first hundred days). Claw skin diseases caused a stronger decrease than claw horn diseases (skin: 600 kg milk, horn: 360 kg milk). Claw health status also had an effect on the 305 - days milk yield: For severe claw disorders a difference of - 1135 kg of milk per 305 - day milk yield could be registered. Again, claw skin diseases led to a higher reduction than claw horn diseases (-949 kg of milk or 522 kg of milk). The milk yield of affected animals housed in TS also tended to be inferior to that of healthy animals, but the effects were statistically not significant (-448 kg milk).","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"12 1","pages":"377-387"},"PeriodicalIF":0.4,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74372122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful treatment of acute portal vein thrombosis after upper abdominal surgery in an Irish-Setter using dalteparin and phenprocoumon","authors":"M. Nolff, S. Hungerbühler, R. Mischke","doi":"10.2376/0005-9366-15101","DOIUrl":"https://doi.org/10.2376/0005-9366-15101","url":null,"abstract":"Portal vein thrombosis has been rarely described in the dog. It had been associated with hepathopathy, nephropathy, steroid administration, immune mediated anaemia, pancreatitis, neoplasia and vasculitis due to chronic ehrlichiosis. This is the first description of treatment of portal vein thrombosis after abdominal surgery using dalteparin and phenprocoumon in a dog. Portal vein thrombosis developed after \"repair of diaphragmatic hernia and small bowel resection in an eight month old Irish-Setter. Thrombosis resulted in ascites formation, portal hypertension, formation of pleural effusion and pancreatic oedema and was treated using dalteparin and phenprocoumon. A loading dose of 0.14 mg/kg BW phenprocoumon once daily on five consecutive days resulted in remarkable changes of the prothrombin time (PT) exceeding the target range. Fortunately, no obvious bleeding or other substantial side effects were observed. The dog showed complete recovery and full re-canalisation of the portal vein was achieved within 19 days. In conclusion, use of phenprocoumon in dogs must be closely monitored with PT and a reduced loading dose should be used in future cases.","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"54 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74839775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitivity of liver parameters in diagnosing liver diseases in rabbits","authors":"A. Leban-Danzl, K. Hartmann, M. Majzoub-Altweck, W. Hermanns, C. Sauter-Louis, J. Hein","doi":"10.2376/0005-9366-15108","DOIUrl":"https://doi.org/10.2376/0005-9366-15108","url":null,"abstract":"This retrospective study evaluated the sensitivity and clinical importance of liver parameters (alanine aminotransferase [ALT], alkaline phosphatase [AP], aspartate aminotransferase [AST], glutamate dehydrogenase [GLDH], γ-glutamyltransferase [GGT], glucose, albumin, total protein, bilirubin, and urea) for diagnosing hepatopathies (hepatic lipidosis, inflammation, diseases of the bile duct, neoplasia, cirrhosis, fibrosis, other liver diseases) in rabbits. The laboratory results of 77 rabbits with hepatopathies diagnosed via cytological or histopathological examination were investigated by assessing frequency distributions, associations between liver parameters and different hepatopathies, and intercorrelations between parameters. The most frequent liver disease was hepatic lipidosis (40/77), followed by inflammation (3/77). Aspartate aminotransferase was the parameter most commonly increased (n = 20/77, 70.0% above the reference interval), whereas AP activity never exceeded the reference interval. Significant (p 0.9) were found between AST/ALT, GGT/ALT, GGT/AST, and bilirubin/GGT, and significant but lower correlations (p ≤ 0.001, r = 0.–0.9) were detected for GLDH/ALT, and GLDH/AST. The study showed that AST, GLDH, ALT, and GGT, in contrast to AP, represent suitable parameters for detecting hepatopathies in rabbits.","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"129 1","pages":"518-526"},"PeriodicalIF":0.4,"publicationDate":"2016-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87598256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frühsommer-Meningoenzephalitis-Virus-Infektionen bei Tieren – Klinik, Diagnostik und epidemiologische Bedeutung","authors":"Christine Klaus, D. Hoffmann, B. Hoffmann, M. Beer","doi":"10.2376/0005-9366-16062","DOIUrl":"https://doi.org/10.2376/0005-9366-16062","url":null,"abstract":"Die Fruhsommer-Meningoenzephalitis (FSME) ist in Europa und Teilen Asiens die bedeutendste durch Zecken ubertragene virale Zoonose mit mehreren Tausend humanen Erkrankungen pro Jahr allein in Europa. FSME-Viren werden meist via Zeckenstich ubertragen, allerdings sind auch alimentare Infektionen uber die Rohmilch viramischer Schafe, Ziegen oder Kuhe oder daraus hergestellter Produkte moglich. Der derzeitige Wissensstand zu FSME-Virus-Infektionen beim Tier wird bezuglich des klinischen Bildes, der Differenzialdiagnose, der Labordiagnostik und der epidemiologischen Bedeutung dargestellt. Zusatzlich werden Empfehlungen zum Umgang mit dieser bei Tieren zwar seltenen, aber dennoch auch im Hinblick auf den Schutz der Verbraucher beachtenswerten Erkrankung gegeben.","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"4 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2016-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83448648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laborvergleichsuntersuchung zum Nachweis von Staphylokokken-Enterotoxinen der Typen SEA-SEE im Lebensmittel in Deutschland im Jahr 2013","authors":"A. Fetsch, K. Steege, D. Leeser, G. Krause","doi":"10.2376/0005-9366-15047","DOIUrl":"https://doi.org/10.2376/0005-9366-15047","url":null,"abstract":"In the autumn 2013, the National Reference Laboratory for coagulase positive staphylococci (CPS) including Staphylococcus (S.) aureus (NRL-Staph) at the Federal Institute for Risk Assessment has organized its first interlaboratory profiency testing (ILPT) trial for the detection of staphylococcal enterotoxins (SE) types SEA to SEE in food. The purpose of the ILPT was to assess the analytical competence of the official laboratories of the Federal German “Lander” authorities. Moreover, it was the intention to gain an overview of the standard methods implemented in the participating laboratories for the purpose of SE detection in food. Five cream cheese samples at three different contamination levels (blank, low, and high) were sent to each participant. In total, 15 laboratories participated to the ILPT: 14 laboratories from 11 Federal German “Lander”, and the European Reference Laboratory for CPS including S. aureus (EU-RL for CPS). Data sets from 14 participating laboratories were included in the analysis. Overall, a specificity of 100% (14/14 true negative results), a sensitivity of 55% (31/56 true positive results), and an accuracy of 64% (45/60 true results) was achieved. The majority of participants (9/15) used other analytical methods for the detection of SE in food than the suggested European Screening Method (ESM) v5. To conclude on the ILPT in general it is to state that the majority of participating laboratories failed to correctly identify SE-low-contaminated samples. Further efforts are necessary to improve the analytical capacity and sensitivity as regards the detection of SE in food in Germany","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"118 1","pages":"290-295"},"PeriodicalIF":0.4,"publicationDate":"2016-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77442160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential role of real-time PCR for detecting Mycobacterium avium subsp. paratuberculosis in chronically diseased milking cows: a case control study.","authors":"Ahmad Alajmi,&nbsp;Günter Klein,&nbsp;Matthias Greiner,&nbsp;Nils Th Grabowski,&nbsp;Svenja Fohler,&nbsp;Amely Campe,&nbsp;Theresa Scheu,&nbsp;Martina Hoedemaker,&nbsp;Amir Abdulmawjood","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP) is economically important to dairy operations. In the present study, a real-time PCR kit (certified for use in Germany) was used to detect MAP in bovine fecal and milk samples within a case control study examining different factors for their association with chronic disease in Northern Germany.The aim of this study was to describe the suitability of the MAP real-time PCR kit to detect MAP in feces and milk of chronically diseased dairy cows. For MAP detection a total of 928 fecal, 922 composite foremilk, and 92 bulk milk samples obtained from 58 cases and 35 control dairy herds were investigated. The real-time PCR showed MAP positive results for 11 (18.96%) and 6 (17.14%) of the case and control herds, respectively. All bulk milk samples were MAP negative. The results of fecal and milk samples were moderately correlated (kappa = 0.27). These data indicate that real-time PCR results have diagnostic value for diagnosing MAP positive animals, and that fecal samples are more suitable than milk samples for assessing the reasons for chronic disease on dairy farms.</p>","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"129 7-8","pages":"304-9"},"PeriodicalIF":0.4,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34312023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seropositivity of Borrelia burgdorferi in a cohort of symptomatic cats from Europe based on a C6-peptide assay with discussion of implications in disease aetiology.","authors":"Nikola Pantchev,&nbsp;Majda Globokar Vrhovec,&nbsp;Silvia Pluta,&nbsp;Reinhard K Straubinger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>There are only few reports on Lyme borreliosis (LB) in cats. The reasons might be a different tick infestation in cats compared to dogs, a low susceptibility for tick-borne infections or a low awareness of veterinarians for tick-borne diseases in feline patients. The aim of this study was to determine the proportion of antibodies against Borrelia burgdorferi sensu lato (Bbsl) in feline sera, to compare the significance of feline versus canine LB, as well as to evaluate possible implications on disease occurrence. Specific antibodies against the C6-peptide of Bbsl in cats were detected by a rapid test based on enzyme immunoassay technique. The serum samples were sent to a diagnostic laboratory by veterinarians from Germany and other European countries with request for Borrelia serology in the years 2009-2011. Veterinarians were asked for information regarding the cats' location, age, gender, clinical signs, treatment and follow-up. In six of 271 (2.2%; 95% CI: 0.8-4.8%) cat sera, antibodies against the C6-peptide of Bbsl were detected. Proportion of Borrelia antibody-positive cat sera was significantly lower than the one determined for dogs during the same time period. All positive cats lived in countries endemic for LB (Germany, Sweden and Belgium), and all C6-antibody positive cats with the exception of one cat showed clinical signs. Possible implications on disease occurrence are discussed. Data presented here demonstrate a lower prevalence of Borrelia specific C6-antibodies in European cats when compared to dogs residing in the same regions. The absence of antibodies against Bbsl in 97.8% (95% CI: 95.2-99.2%) of the submitted samples indicate that diagnosis \"feline LB\"is rare in cats. Nevertheless, LB should be considered in cats with compatible clinical signs (e.g. shifting leg lameness, to less extent neurological signs) when other differential diagnoses are ruled out.</p>","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"129 7-8","pages":"333-9"},"PeriodicalIF":0.4,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34312492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous occurrence of Salmonella enterica, Campylobacter spp. and Yersinia enterocolitica along the pork production chain from farm to meat processing in five conventional fattening pig herds in Lower Saxony.","authors":"Jana-Kristin Niemann,&nbsp;Thomas Alter,&nbsp;Greta Gölz,&nbsp;Erhard Tietze,&nbsp;Angelika Fruth,&nbsp;Wolfgang Rabsch,&nbsp;Christiane von Münchhausen,&nbsp;Roswitha Merle,&nbsp;Lothar Kreienbrock","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The objectives of this study were to gather data on the occurrence of Salmonella (S.) enterica, Campylobacter spp. and Yersinia (Y.) enterocolitica along the pork production chain and to further analyze detected Salmonella isolates by additionally applying MLVA (multiple-locus variable-number tandem repeat analysis). In total, 336 samples were collected at primary production, slaughter and meat processing from five conventional fattening pig farms and one common slaughterhouse. At farm level, S. enterica, Campylobacter spp. and Y. enterocolitica were detected in 19.4%, 38.9% and 11.1% of pooled fecal samples of fattening pigs. At slaughter, more than two-thirds of examined carcasses, 24% of carcass surfaces samples and about 60% of cecal content samples were positive for at least one of the examined pathogens. An amount of 4% of meat samples were positive for non-human pathogenic Y. enterocolitica. Identical MLVA patterns of Salmonella isolates from farm- and associated slaughterhouse samples demonstrated transmission across both production stages. Other MLVA patterns found at slaughter indicated possible colonization of pigs during transport or lairage and/or cross-contamination during slaughter. Identical MLVA patterns from risk tissues and the nearby carcass surface evidenced a direct contamination of carcasses as well. Overall, our data showed wide distribution ranges for all three examined pathogens within the pig production chain and underline the need for appropriate intervention strategies at pre- and postharvest.</p>","PeriodicalId":8761,"journal":{"name":"Berliner und Munchener tierarztliche Wochenschrift","volume":"129 7-8","pages":"296-303"},"PeriodicalIF":0.4,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34312022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}