Biochimica et biophysica acta. Molecular cell research最新文献

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FAM188B promotes the growth, metastasis, and invasion of hepatocellular carcinoma by targeting the hnRNPA1/PKM2 axis FAM188B 通过靶向 HRNPA1/PKM2 轴促进肝细胞癌的生长、转移和侵袭。
IF 5.1 2区 生物学
Biochimica et biophysica acta. Molecular cell research Pub Date : 2024-06-04 DOI: 10.1016/j.bbamcr.2024.119773
Mingshan Mu , Yisong Lu , Kangsheng Tu , Linglan Tu , Chaoqin Guo , Zilin Li , Xu Zhang , Yihong Chen , Xin Liu , Qiuran Xu , Dongsheng Huang , Xiaoyan Li
{"title":"FAM188B promotes the growth, metastasis, and invasion of hepatocellular carcinoma by targeting the hnRNPA1/PKM2 axis","authors":"Mingshan Mu ,&nbsp;Yisong Lu ,&nbsp;Kangsheng Tu ,&nbsp;Linglan Tu ,&nbsp;Chaoqin Guo ,&nbsp;Zilin Li ,&nbsp;Xu Zhang ,&nbsp;Yihong Chen ,&nbsp;Xin Liu ,&nbsp;Qiuran Xu ,&nbsp;Dongsheng Huang ,&nbsp;Xiaoyan Li","doi":"10.1016/j.bbamcr.2024.119773","DOIUrl":"10.1016/j.bbamcr.2024.119773","url":null,"abstract":"<div><p>Hepatocellular carcinoma (HCC), the leading cause of cancer-related deaths worldwide, is characterised by rapid growth and marked invasiveness. Accumulating evidence suggests that deubiquitinases play a pivotal role in HCC growth and metastasis. However, the expression of the deubiquitinase FAM188B and its biological functions in HCC remain unknown. The aim of our study was to investigate the potential role of FAM188B in HCC. The expression of FAM188B was significantly upregulated in liver cancer cells compared to normal liver cells, both at the transcriptional and translational levels. Similarly, FAM188B expression was higher in liver cancer tissues than in normal liver tissues. Bioinformatic analysis revealed that high FAM188B expression was associated with poor prognosis in patients with HCC. We further demonstrated that FAM188B knockdown inhibited cell proliferation, epithelial-mesenchymal transition, migration and invasion both in vitro and in vivo. Mechanistically, FAM188B knockdown significantly inhibited the hnRNPA1/PKM2 pathway in HCC cells. FAM188B may inhibit ubiquitin-mediated degradation of hnRNPA1 through deubiquitination. Notably, we observed that the inhibitory effects of FAM188B knockdown on HCC cell proliferation, migration and invasion were reversed when hnRNPA1 expression was restored. In conclusion, FAM188B promotes HCC progression by enhancing the deubiquitination of hnRNPA1 and subsequently activating the hnRNPA1/PKM2 pathway. Therefore, targeting FAM188B is a potential strategy for HCC therapy.</p></div>","PeriodicalId":8754,"journal":{"name":"Biochimica et biophysica acta. Molecular cell research","volume":"1871 7","pages":"Article 119773"},"PeriodicalIF":5.1,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141282911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tumour suppressor protein sMEK1 links to IRE1 signalling pathway to modulate its activity during ER stress 肿瘤抑制蛋白 sMEK1 与 IRE1 信号通路相连,可在 ER 应激时调节其活性。
IF 5.1 2区 生物学
Biochimica et biophysica acta. Molecular cell research Pub Date : 2024-06-04 DOI: 10.1016/j.bbamcr.2024.119774
Ozaira Qadri , Samirul Bashir , Mariam Banday , Nazia Hilal , Younis Majeed , Nida I Fatima , Debnath Pal , Khalid Majid Fazili
{"title":"Tumour suppressor protein sMEK1 links to IRE1 signalling pathway to modulate its activity during ER stress","authors":"Ozaira Qadri ,&nbsp;Samirul Bashir ,&nbsp;Mariam Banday ,&nbsp;Nazia Hilal ,&nbsp;Younis Majeed ,&nbsp;Nida I Fatima ,&nbsp;Debnath Pal ,&nbsp;Khalid Majid Fazili","doi":"10.1016/j.bbamcr.2024.119774","DOIUrl":"10.1016/j.bbamcr.2024.119774","url":null,"abstract":"<div><p>The Endoplasmic Reticulum is a pervasive, dynamic cellular organelle that performs a wide range of functions in the eukaryotic cell, including protein folding and maturation. Upon stress, ER activates an adaptive cellular pathway, namely Unfolded Protein Response, that transduces information from ER to nucleus, restoring homeostasis in the ER milieu. UPR consists of three membrane-tethered sensors; IRE1, PERK and ATF6. Among all the UPR sensors, the IRE1 branch acts as a central pathway that orchestrates several pathways to determine cell fate. However, the detailed knowledge underlying the whole process is not understood yet. Previously, we determined the sMEK1 as one of the interacting partners of IRE1. sMEK1 is a protein phosphatase, which has been indicated in a number of critical cellular functions like apoptosis, cell proliferation, and tumour suppression. In this study, we evaluated the role of sMEK1 on the IRE1 signalling pathway. Our data indicate that sMEK1 can inhibit IRE1 phosphorylation under ER stress. This inhibitory effect of sMEK1 could be reflected in its downstream effectors, Xbp1 and RIDD, which are downregulated in the presence of sMEK1. We also found that the repressing effect of sMEK1 was specific to the IRE1 signalling pathway and could be preserved even under prolonged ER stress. Our findings also indicate that sMEK1 can inhibit IRE1 and its downstream molecules under ER stress irrespective of other UPR sensors. These results help to draw the mechanistic details giving insights into different molecular connections of UPR with other pathways.</p></div>","PeriodicalId":8754,"journal":{"name":"Biochimica et biophysica acta. Molecular cell research","volume":"1871 7","pages":"Article 119774"},"PeriodicalIF":5.1,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141260229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Suppression of GATA3 promotes epithelial-mesenchymal transition and simultaneous cellular senescence in human extravillous trophoblasts 抑制 GATA3 可促进上皮-间质转化,同时促进人体外滋养细胞的衰老。
IF 5.1 2区 生物学
Biochimica et biophysica acta. Molecular cell research Pub Date : 2024-06-04 DOI: 10.1016/j.bbamcr.2024.119768
En-Xiang Chen , Si-Chen Hu , Jia-Qi Xu , Kun-Yan Liu , Jing Tang , Xi-Peng Shen , Xiao Liang , You-Long Xie , Lu-Xin Ge , Xin Luo , Ying-Xiong Wang , Yun-Long Xiang , Yu-Bin Ding
{"title":"Suppression of GATA3 promotes epithelial-mesenchymal transition and simultaneous cellular senescence in human extravillous trophoblasts","authors":"En-Xiang Chen ,&nbsp;Si-Chen Hu ,&nbsp;Jia-Qi Xu ,&nbsp;Kun-Yan Liu ,&nbsp;Jing Tang ,&nbsp;Xi-Peng Shen ,&nbsp;Xiao Liang ,&nbsp;You-Long Xie ,&nbsp;Lu-Xin Ge ,&nbsp;Xin Luo ,&nbsp;Ying-Xiong Wang ,&nbsp;Yun-Long Xiang ,&nbsp;Yu-Bin Ding","doi":"10.1016/j.bbamcr.2024.119768","DOIUrl":"10.1016/j.bbamcr.2024.119768","url":null,"abstract":"<div><p>The regulatory mechanism of the transcription factor GATA3 in the differentiation and maturation process of extravillous trophoblasts (EVT) in early pregnancy placenta, as well as its relevance to the occurrence of pregnancy disorders, remains poorly understood. This study leveraged single-cell RNA sequencing data from placental organoid models and placental tissue to explore the dynamic changes in GATA3 expression during EVT maturation. The expression pattern exhibited an initial upregulation followed by subsequent downregulation, with aberrant GATA3 localization observed in cases of recurrent miscarriage (RM). By identifying global targets regulated by GATA3 in primary placental EVT cells, JEG3, and HTR8/SVneo cell lines, this study offered insights into its regulatory mechanisms across different EVT cell models. Shared regulatory targets among these cell types and activation of trophoblast cell marker genes emphasized the importance of GATA3 in EVT differentiation and maturation. Knockdown of GATA3 in JEG3 cells led to repression of GATA3-induced epithelial-mesenchymal transition (EMT), as evidenced by changes in marker gene expression levels and enhanced migration ability. Additionally, interference with GATA3 accelerated cellular senescence, as indicated by reduced proliferation rates and increased activity levels for senescence-associated β-galactosidase enzyme, along with elevated expression levels for senescence-associated genes. This study provides comprehensive insights into the dual role of GATA3 in regulating EMT and cellular senescence during EVT differentiation, shedding light on the dynamic changes in GATA3 expression in normal and pathological placental conditions.</p></div>","PeriodicalId":8754,"journal":{"name":"Biochimica et biophysica acta. Molecular cell research","volume":"1871 7","pages":"Article 119768"},"PeriodicalIF":5.1,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141260222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ARRB1 inhibits extracellular matrix degradation and apoptosis of nucleus pulposus cells by promoting autophagy and attenuates intervertebral disc degeneration ARRB1 通过促进自噬抑制细胞外基质降解和髓核细胞凋亡,并减轻椎间盘退变。
IF 5.1 2区 生物学
Biochimica et biophysica acta. Molecular cell research Pub Date : 2024-06-04 DOI: 10.1016/j.bbamcr.2024.119769
Xuejian Dan , Xiaochuan Gu , Ying Zi , Jiahui Xu , Chenggang Wang , Chen Li , Xiao Hu , Zhourui Wu , Yan Yu , Bin Ma
{"title":"ARRB1 inhibits extracellular matrix degradation and apoptosis of nucleus pulposus cells by promoting autophagy and attenuates intervertebral disc degeneration","authors":"Xuejian Dan ,&nbsp;Xiaochuan Gu ,&nbsp;Ying Zi ,&nbsp;Jiahui Xu ,&nbsp;Chenggang Wang ,&nbsp;Chen Li ,&nbsp;Xiao Hu ,&nbsp;Zhourui Wu ,&nbsp;Yan Yu ,&nbsp;Bin Ma","doi":"10.1016/j.bbamcr.2024.119769","DOIUrl":"10.1016/j.bbamcr.2024.119769","url":null,"abstract":"<div><h3>Objective</h3><p>Intervertebral disc degeneration (IVDD) is the leading cause of lower back pain (LBP). β-arrestin 1 (ARRB1) is a multifunctional protein that regulates numerous pathological processes. The aim of this study was to investigate the role of ARRB1 in IVDD.</p></div><div><h3>Methods</h3><p>The expression of ARRB1 in nucleus pulposus (NP) of rats with IVDD was assayed. Next, rat nucleus pulposus cells (NPCs) were infected with lentiviruses containing sh<em>Arrb1</em> (LV-sh<em>Arrb1</em>) and overexpressing <em>Arrb1</em> (LV-oe<em>Arrb1</em>). The roles of <em>Arrb1</em> in serum-deprived NPCs were investigated by measuring apoptosis, extracellular matrix degradation, and autophagic flux. For experiments <em>in vivo</em>, LV-oe<em>Arrb1</em> lentivirus was injected into the NP tissues of IVDD rats to evaluate the effects of <em>Arrb1</em> overexpression on NP.</p></div><div><h3>Results</h3><p>In the NP tissues of IVDD rats, ARRB1 and cleaved caspase-3 expression increased, and the ratio of LC3II/LC3I protein expression was upregulated. <em>Arrb1</em> knockdown aggravated extracellular matrix degradation, cellular apoptosis, and impairment of autophagic flux in rat NPCs under serum-deprived conditions, whereas <em>Arrb1</em> overexpression significantly reversed these effects. ARRB1 interacted with Beclin 1, and <em>Arrb1</em> knockdown suppressed the formation of the Beclin1-PIK3C3 core complex. The autophagy inhibitor 3-methyladenine (3-MA) offset the protective effects of <em>Arrb1</em> overexpression in serum-deprived NPCs. Furthermore, <em>Arrb1</em> overexpression inhibited apoptosis and extracellular matrix degradation, promoted autophagy in NP, and delayed the development of IVDD in rats.</p></div><div><h3>Conclusion</h3><p>ARRB1 prevents extracellular matrix degradation and apoptosis of NPCs by upregulating autophagy and ameliorating IVDD progression, presenting an innovative strategy for the treatment of IVDD.</p></div>","PeriodicalId":8754,"journal":{"name":"Biochimica et biophysica acta. Molecular cell research","volume":"1871 7","pages":"Article 119769"},"PeriodicalIF":5.1,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to “MageC2 protein is upregulated by oncogenic activation of MAPK pathway and causes impairment of the p53 transactivation function” [Biochim. Biophys. Acta, Mol. Cell Res. vol. 1868 (Issue 3) (March 2021) 118918] 更正:"MageC2 蛋白因致癌物质激活 MAPK 通路而上调,并导致 p53 转录激活功能受损" [Biochim.Biophys.Acta, Mol.1868 (Issue 3) (March 2021) 118918]
IF 5.1 2区 生物学
Biochimica et biophysica acta. Molecular cell research Pub Date : 2024-06-03 DOI: 10.1016/j.bbamcr.2024.119767
Franco Andrés Pascucci, María Fátima Ladelfa, María Fernanda Toledo, Micaela Escalada, Melisa Suberbordes, Martín Monte
{"title":"Corrigendum to “MageC2 protein is upregulated by oncogenic activation of MAPK pathway and causes impairment of the p53 transactivation function” [Biochim. Biophys. Acta, Mol. Cell Res. vol. 1868 (Issue 3) (March 2021) 118918]","authors":"Franco Andrés Pascucci,&nbsp;María Fátima Ladelfa,&nbsp;María Fernanda Toledo,&nbsp;Micaela Escalada,&nbsp;Melisa Suberbordes,&nbsp;Martín Monte","doi":"10.1016/j.bbamcr.2024.119767","DOIUrl":"https://doi.org/10.1016/j.bbamcr.2024.119767","url":null,"abstract":"","PeriodicalId":8754,"journal":{"name":"Biochimica et biophysica acta. Molecular cell research","volume":"1871 6","pages":"Article 119767"},"PeriodicalIF":5.1,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0167488924001101/pdfft?md5=5a61249618521fc90ba66bc1b14c9f22&pid=1-s2.0-S0167488924001101-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141243164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fe-S biogenesis by SMS and SUF pathways: A focus on the assembly step SMS 和 SUF 途径的 Fe-S 生物生成:聚焦组装步骤。
IF 4.6 2区 生物学
Biochimica et biophysica acta. Molecular cell research Pub Date : 2024-06-03 DOI: 10.1016/j.bbamcr.2024.119772
Macha Dussouchaud , Frédéric Barras , Sandrine Ollagnier de Choudens
{"title":"Fe-S biogenesis by SMS and SUF pathways: A focus on the assembly step","authors":"Macha Dussouchaud ,&nbsp;Frédéric Barras ,&nbsp;Sandrine Ollagnier de Choudens","doi":"10.1016/j.bbamcr.2024.119772","DOIUrl":"10.1016/j.bbamcr.2024.119772","url":null,"abstract":"<div><p>Fe<img>S clusters are prosthetic groups present in all organisms. Proteins with Fe<img>S centers are involved in most cellular processes. ISC and SUF are machineries necessary for the formation and insertion of Fe<img>S in proteins. Recently, a phylogenetic analysis on more than 10,000 genomes of prokaryotes have uncovered two new systems, MIS and SMS, which were proposed to be ancestral to ISC and SUF. SMS is composed of SmsBC, two homologs of SufBC(D), the scaffolding complex of SUF. In this review, we will specifically focus on the current knowledge of the SUF system and on the new perspectives given by the recent discovery of its ancestor, the SMS system.</p></div>","PeriodicalId":8754,"journal":{"name":"Biochimica et biophysica acta. Molecular cell research","volume":"1871 7","pages":"Article 119772"},"PeriodicalIF":4.6,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0167488924001150/pdfft?md5=33bc6b5ef968b753dd8a6c051d252fdc&pid=1-s2.0-S0167488924001150-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Wnt/β-catenin signaling inhibits oxidative stress-induced ferroptosis to improve interstitial cystitis/bladder pain syndrome by reducing NF-κB Wnt/β-catenin信号通过减少NF-κB抑制氧化应激诱导的铁氧化,从而改善间质性膀胱炎/膀胱疼痛综合征。
IF 4.6 2区 生物学
Biochimica et biophysica acta. Molecular cell research Pub Date : 2024-05-31 DOI: 10.1016/j.bbamcr.2024.119766
Weilin Fang , Xin Song , Hailong Li , Fanguo Meng , Tingting Lv , Jin Huang , Xiang Ji , Jianwei Lv , Zhikang Cai , Zhong Wang
{"title":"Wnt/β-catenin signaling inhibits oxidative stress-induced ferroptosis to improve interstitial cystitis/bladder pain syndrome by reducing NF-κB","authors":"Weilin Fang ,&nbsp;Xin Song ,&nbsp;Hailong Li ,&nbsp;Fanguo Meng ,&nbsp;Tingting Lv ,&nbsp;Jin Huang ,&nbsp;Xiang Ji ,&nbsp;Jianwei Lv ,&nbsp;Zhikang Cai ,&nbsp;Zhong Wang","doi":"10.1016/j.bbamcr.2024.119766","DOIUrl":"10.1016/j.bbamcr.2024.119766","url":null,"abstract":"<div><h3>Background</h3><p>Interstitial cystitis/bladder pain syndrome (IC/BPS) is a bladder syndrome of unknown etiology. Reactive oxygen species (ROS) plays a major role in ferroptosis and bladder dysfunction of IC/BPS, while the role of ferroptosis in IC/BPS progression is still unclear. This study aims to investigate the role and mechanism of ROS-induced ferroptosis in IC/BPS using cell and rat model.</p></div><div><h3>Methods</h3><p>We collected IC/BPS patient bladder tissue samples and established a LPS-induced IC/BPS rat model (LRM). The level of oxidative stress and ferroptosis in IC/BPS patients and LRM rats was analyzed. Function and regulatory mechanism of ferroptosis in IC/BPS were explored by <em>in vitro</em> and <em>in vivo</em> experiments.</p></div><div><h3>Results</h3><p>The patients with IC/BPS showed mast cells and inflammatory cells infiltration in bladder epithelial tissues. Expression of NRF2 was up-regulated, and GPX4 was decreased in IC/BPS patients compared with normal tissues. IC model cells underwent oxidative stress, which induced ferroptosis. These above results were validated in LRM rat models, and inhibition of ferroptosis ameliorated bladder dysfunction in LRM rats. Wnt/β-catenin signaling was deactivated in IC/BPS patients and animals, and activation of Wnt/β-catenin signaling reduced cellular free radical production, thereby inhibited ferroptosis in IC model cells. Mechanistically, the Wnt/β-catenin signaling pathway inhibited oxidative stress-induced ferroptosis by down-regulating NF-κB, thus contributing to recover IC/BPS both <em>in vitro</em> and <em>in vivo</em>.</p></div><div><h3>Conclusions</h3><p>We demonstrate for the first time that oxidative stress-induced ferroptosis plays an important role in the pathology of IC/BPS. Mechanistically, the Wnt/β-catenin signaling suppressed oxidative stress-induced ferroptosis by down-regulating NF-κB to improve bladder injury in IC/BPS.</p></div>","PeriodicalId":8754,"journal":{"name":"Biochimica et biophysica acta. Molecular cell research","volume":"1871 7","pages":"Article 119766"},"PeriodicalIF":4.6,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141185997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HRD1-mediated ubiquitination of HDAC2 regulates PPARα-mediated autophagy and alleviates metabolic-associated fatty liver disease HRD1 介导的 HDAC2 泛素化调节 PPARα 介导的自噬,缓解代谢相关性脂肪肝。
IF 5.1 2区 生物学
Biochimica et biophysica acta. Molecular cell research Pub Date : 2024-05-28 DOI: 10.1016/j.bbamcr.2024.119765
Yina Wang , Yuanguo Chen , Xiao Xiao , Silei Deng , Jingjie Kuang , Yayong Li
{"title":"HRD1-mediated ubiquitination of HDAC2 regulates PPARα-mediated autophagy and alleviates metabolic-associated fatty liver disease","authors":"Yina Wang ,&nbsp;Yuanguo Chen ,&nbsp;Xiao Xiao ,&nbsp;Silei Deng ,&nbsp;Jingjie Kuang ,&nbsp;Yayong Li","doi":"10.1016/j.bbamcr.2024.119765","DOIUrl":"10.1016/j.bbamcr.2024.119765","url":null,"abstract":"<div><h3>Background</h3><p>Metabolic-associated fatty liver disease (MAFLD) is a leading cause of chronic liver disease worldwide. Autophagy plays a pivotal role in lipid metabolism; however, the mechanism underlying the reduced autophagic activity in MAFLD remains elusive.</p></div><div><h3>Methods</h3><p>Autophagy was monitored by TUNEL assay and immunofluorescence staining of LC3. The expression of autophagy-related proteins, PPARα, HDAC2, and HRD1 was detected by Western blot. The association between HDAC2 and PPARα promoter was assessed by chromatin immunoprecipitation (ChIP) and dual-luciferase assays, and the HRD1-mediated ubiquitin-proteasomal degradation of HDAC2 was detected by co-immunoprecipitation (co-IP). The in vitro findings were validated in a hypoxia-induced MAFLD mouse model. Histological changes, fibrosis, and apoptosis in liver tissues were detected by hematoxylin and eosin staining, Masson's trichrome staining, and TUNEL assay. The immunoreactivities of key molecules were examined by IHC analysis.</p></div><div><h3>Results</h3><p>Hypoxia-suppressed autophagy in hepatocytes. Hypoxic exposure downregulated HRD1 and PPARα, while upregulating HDAC2 in hepatocytes. Overexpression of PPARα promoted hepatic autophagy, while knocking down HDAC2 or overexpressing HRD1 reduced hypoxia-suppressed autophagy in hepatocytes. Mechanistically, HDAC2 acted as a transcriptional repressor of PPARα, and HRD1 mediated the degradation of HDAC2 through the ubiquitin-proteasome pathway. Functional studies further showed that hypoxia-suppressed hepatic autophagy via the HRD1/HDAC2/PPARα axis in vitro and in vivo.</p></div><div><h3>Conclusion</h3><p>HRD1-mediated ubiquitination of HDAC2 regulates PPARα-mediated autophagy and ameliorates hypoxia-induced MAFLD.</p></div>","PeriodicalId":8754,"journal":{"name":"Biochimica et biophysica acta. Molecular cell research","volume":"1871 7","pages":"Article 119765"},"PeriodicalIF":5.1,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141178283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Akkermansia muciniphila ameliorates colonic injury in mice with DSS-induced acute colitis by blocking macrophage pro-inflammatory phenotype switching via the HDAC5/DAB2 axis Akkermansia muciniphila通过HDAC5/DAB2轴阻断巨噬细胞促炎表型转换,从而改善DSS诱导的急性结肠炎小鼠的结肠损伤。
IF 5.1 2区 生物学
Biochimica et biophysica acta. Molecular cell research Pub Date : 2024-05-21 DOI: 10.1016/j.bbamcr.2024.119751
Yan Miao , Mian Wang , Hao Sun , Yujie Zhang , Wei Zhou , Wanli Yang , Lili Duan , Liaoran Niu , Zhenshun Li , Junfeng Chen , Yiding Li , Aqiang Fan , Qibin Xie , Siyu Wei , Han Bai , Chenyang Wang , Qian Chen , Xiangjie Wang , Yunlong Li , Jinqiang Liu , Liu Hong
{"title":"Akkermansia muciniphila ameliorates colonic injury in mice with DSS-induced acute colitis by blocking macrophage pro-inflammatory phenotype switching via the HDAC5/DAB2 axis","authors":"Yan Miao ,&nbsp;Mian Wang ,&nbsp;Hao Sun ,&nbsp;Yujie Zhang ,&nbsp;Wei Zhou ,&nbsp;Wanli Yang ,&nbsp;Lili Duan ,&nbsp;Liaoran Niu ,&nbsp;Zhenshun Li ,&nbsp;Junfeng Chen ,&nbsp;Yiding Li ,&nbsp;Aqiang Fan ,&nbsp;Qibin Xie ,&nbsp;Siyu Wei ,&nbsp;Han Bai ,&nbsp;Chenyang Wang ,&nbsp;Qian Chen ,&nbsp;Xiangjie Wang ,&nbsp;Yunlong Li ,&nbsp;Jinqiang Liu ,&nbsp;Liu Hong","doi":"10.1016/j.bbamcr.2024.119751","DOIUrl":"10.1016/j.bbamcr.2024.119751","url":null,"abstract":"<div><p><em>Akkermansia muciniphila</em> (<em>A. muciniphila</em>), a probiotic, has been linked to macrophage phenotypic polarization in different diseases. However, the role and mechanisms of <em>A. muciniphila</em> in regulating macrophage during ulcerative colitis (UC) are not clear. This research aimed to examine the impact of <em>A. muciniphila</em> on dextran sulfate sodium (DSS)-induced acute colitis and elucidate the underlying mechanism related to macrophage phenotypic polarization. <em>A. muciniphila</em> inhibited weight loss, increased disease activity index, and ameliorated inflammatory injury in colonic tissues in mice induced with DSS. Furthermore, <em>A. muciniphila</em> reduced macrophage M1 polarization and ameliorated epithelial barrier damage in colonic tissues of DSS-induced mice through inhibition of histone deacetylase 5 (HDAC5). In contrast, the effect of <em>A. muciniphila</em> was compromised by HDAC5 overexpression. HDAC5 deacetylated H3K9ac modification of the disabled homolog 2 (DAB2) promoter, which led to repressed DAB2 expression. DAB2 overexpression blocked HDAC5-induced pro-inflammatory polarization of macrophages, whereas knockdown of DAB2 resulted in the loss of effects of <em>A. muciniphila</em> against colonic injury in DSS-induced mice. Taken together, <em>A. muciniphila</em>-induced loss of HDAC5 hampered the deacetylation of DAB2 and enhanced the expression of DAB2. Our findings propose that <em>A. muciniphila</em> may be a possible probiotic agent for alleviating DSS-induced acute colitis.</p></div>","PeriodicalId":8754,"journal":{"name":"Biochimica et biophysica acta. Molecular cell research","volume":"1871 7","pages":"Article 119751"},"PeriodicalIF":5.1,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mitophagy at the crossroads of cancer development: Exploring the role of mitophagy in tumor progression and therapy resistance 癌症发展十字路口的有丝分裂:探索有丝分裂在肿瘤进展和耐药性中的作用。
IF 5.1 2区 生物学
Biochimica et biophysica acta. Molecular cell research Pub Date : 2024-05-20 DOI: 10.1016/j.bbamcr.2024.119752
K. Deepak , Pritam Kumar Roy , Chandan Kanta Das , Budhaditya Mukherjee , Mahitosh Mandal
{"title":"Mitophagy at the crossroads of cancer development: Exploring the role of mitophagy in tumor progression and therapy resistance","authors":"K. Deepak ,&nbsp;Pritam Kumar Roy ,&nbsp;Chandan Kanta Das ,&nbsp;Budhaditya Mukherjee ,&nbsp;Mahitosh Mandal","doi":"10.1016/j.bbamcr.2024.119752","DOIUrl":"10.1016/j.bbamcr.2024.119752","url":null,"abstract":"<div><p>Preserving a functional mitochondrial network is crucial for cellular well-being, considering the pivotal role of mitochondria in ensuring cellular survival, especially under stressful conditions. Mitophagy, the selective removal of damaged mitochondria through autophagy, plays a pivotal role in preserving cellular homeostasis by preventing the production of harmful reactive oxygen species from dysfunctional mitochondria. While the involvement of mitophagy in neurodegenerative diseases has been thoroughly investigated, it is becoming increasingly evident that mitophagy plays a significant role in cancer biology. Perturbations in mitophagy pathways lead to suboptimal mitochondrial quality control, catalyzing various aspects of carcinogenesis, including establishing metabolic plasticity, stemness, metabolic reconfiguration of cancer-associated fibroblasts, and immunomodulation. While mitophagy performs a delicate balancing act at the intersection of cell survival and cell death, mounting evidence indicates that, particularly in the context of stress responses induced by cancer therapy, it predominantly promotes cell survival. Here, we showcase an overview of the current understanding of the role of mitophagy in cancer biology and its potential as a target for cancer therapy. Gaining a more comprehensive insight into the interaction between cancer therapy and mitophagy has the potential to reveal novel targets and pathways, paving the way for enhanced treatment strategies for therapy-resistant tumors in the near future.</p></div>","PeriodicalId":8754,"journal":{"name":"Biochimica et biophysica acta. Molecular cell research","volume":"1871 6","pages":"Article 119752"},"PeriodicalIF":5.1,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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