催化蛋白酶体亚基在人血小板中具有异质性活性，β1i在信号传导和颗粒释放中发挥作用

IF 3.7 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. Molecular cell research Pub Date : 2025-04-15 DOI:10.1016/j.bbamcr.2025.119961

Lara Smrdel , Slavica Stanišić , Živa Zajec , Martina Gobec

{"title":"催化蛋白酶体亚基在人血小板中具有异质性活性，β1i在信号传导和颗粒释放中发挥作用","authors":"Lara Smrdel , Slavica Stanišić , Živa Zajec , Martina Gobec","doi":"10.1016/j.bbamcr.2025.119961","DOIUrl":null,"url":null,"abstract":"<div><div><ul><li><span>•</span><span><div>Human platelets contain catalytically active subunits of both constitutive and immunoproteasomes, with predominant expression of β5i, β5c, β1i, and β1c subunits.</div></span></li><li><span>•</span><span><div>Analysis of 31 healthy donors revealed substantial interindividual variability in both the protein levels and active states of proteasome subunits in platelets.</div></span></li><li><span>•</span><span><div>The proteasome catalytic subunit β1i was found to play a significant role in regulating platelet signaling pathways, particularly influencing the p38 and NFкB pathway.</div></span></li><li><span>•</span><span><div>Inhibition of β1i enhanced α-granule release, notably increasing CCL5 secretion, upon ADP activation, highlighting its potential role in platelet activation and immune regulation.</div></span></li></ul></div></div>","PeriodicalId":8754,"journal":{"name":"Biochimica et biophysica acta. Molecular cell research","volume":"1872 5","pages":"Article 119961"},"PeriodicalIF":3.7000,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Catalytic proteasome subunits are heterogeneously active in human platelets with β1i playing a role in signaling and granule release\",\"authors\":\"Lara Smrdel , Slavica Stanišić , Živa Zajec , Martina Gobec\",\"doi\":\"10.1016/j.bbamcr.2025.119961\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><ul><li><span>•</span><span><div>Human platelets contain catalytically active subunits of both constitutive and immunoproteasomes, with predominant expression of β5i, β5c, β1i, and β1c subunits.</div></span></li><li><span>•</span><span><div>Analysis of 31 healthy donors revealed substantial interindividual variability in both the protein levels and active states of proteasome subunits in platelets.</div></span></li><li><span>•</span><span><div>The proteasome catalytic subunit β1i was found to play a significant role in regulating platelet signaling pathways, particularly influencing the p38 and NFкB pathway.</div></span></li><li><span>•</span><span><div>Inhibition of β1i enhanced α-granule release, notably increasing CCL5 secretion, upon ADP activation, highlighting its potential role in platelet activation and immune regulation.</div></span></li></ul></div></div>\",\"PeriodicalId\":8754,\"journal\":{\"name\":\"Biochimica et biophysica acta. Molecular cell research\",\"volume\":\"1872 5\",\"pages\":\"Article 119961\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-04-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et biophysica acta. Molecular cell research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0167488925000667\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et biophysica acta. Molecular cell research","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167488925000667","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

•人血小板含有催化活性的组成和免疫蛋白酶体亚基，主要表达β5i、β5c、β1i和β1c亚基。•对31名健康供体的分析显示，血小板中蛋白酶体亚基的蛋白质水平和活性状态存在显著的个体差异。•发现蛋白酶体催化亚基β1i在调节血小板信号通路中发挥重要作用，特别是影响p38和NFкB通路。•抑制β1i增强了α-颗粒的释放，特别是在ADP激活时增加CCL5的分泌，突出了其在血小板激活和免疫调节中的潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Catalytic proteasome subunits are heterogeneously active in human platelets with β1i playing a role in signaling and granule release

•
Human platelets contain catalytically active subunits of both constitutive and immunoproteasomes, with predominant expression of β5i, β5c, β1i, and β1c subunits.
•
Analysis of 31 healthy donors revealed substantial interindividual variability in both the protein levels and active states of proteasome subunits in platelets.
•
The proteasome catalytic subunit β1i was found to play a significant role in regulating platelet signaling pathways, particularly influencing the p38 and NFкB pathway.
•
Inhibition of β1i enhanced α-granule release, notably increasing CCL5 secretion, upon ADP activation, highlighting its potential role in platelet activation and immune regulation.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biochimica et biophysica acta. Molecular cell research 生物-生化与分子生物学

CiteScore

10.00

自引率

2.00%

发文量

151

审稿时长

44 days

期刊介绍： BBA Molecular Cell Research focuses on understanding the mechanisms of cellular processes at the molecular level. These include aspects of cellular signaling, signal transduction, cell cycle, apoptosis, intracellular trafficking, secretory and endocytic pathways, biogenesis of cell organelles, cytoskeletal structures, cellular interactions, cell/tissue differentiation and cellular enzymology. Also included are studies at the interface between Cell Biology and Biophysics which apply for example novel imaging methods for characterizing cellular processes.