{"title":"Drug-target discovery in silico: using the web to identify novel molecular targets for drug action.","authors":"David S Wishart","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":87484,"journal":{"name":"SEB experimental biology series","volume":"58 ","pages":"145-76"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26811320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
David B Sattelle, Andrew K Jones, Laurence A Brown, Steven D Buckingham, Christopher J Mee, Luanda Pym
{"title":"Nicotinic acetylcholine receptors as drug/chemical targets, contributions from comparative genomics, forward and reverse genetics.","authors":"David B Sattelle, Andrew K Jones, Laurence A Brown, Steven D Buckingham, Christopher J Mee, Luanda Pym","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":87484,"journal":{"name":"SEB experimental biology series","volume":"58 ","pages":"93-113"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26811317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Frauz Conen, Argyro Zerva, Dominique Arrouays, Claude Jolivet, Paul G Jarvis, John Grace, Maurizio Mencuccini
{"title":"The carbon balance of forest soils: detectability of changes in soil carbon stocks in temperate and Boreal forests.","authors":"Frauz Conen, Argyro Zerva, Dominique Arrouays, Claude Jolivet, Paul G Jarvis, John Grace, Maurizio Mencuccini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Estimating soil carbon content as the product of mean carbon concentration and bulk density can result in considerable overestimation. Carbon concentration and soil mass need to be measured on the same sample and carbon contents calculated for each individual sample before averaging. The effect of this bias is likely to be smaller (but still greater than zero) when the primary objective is to determine stock changes over time. Variance and mean carbon content are significantly and positively related to each other, although some sites showed much higher variability than predicted by this relationship, as a likely consequence of their particular site history, forest management, and micro-topography. Because of the proportionality between mean and variance, the number of samples required to detect a fixed change in soil carbon stocks varied directly with the site mean carbon content from less than 10 to several thousands across the range of carbon stocks normally encountered in temperate and Boreal forests. This raises important questions about how to derive an optimal sampling strategy across such a varied range of conditions so as to achieve the aims of the Kyoto Protocol. Overall, on carbon-poor forest sites with little or no disturbance to the soil profile, it is possible to detect changes in total soil organic carbon over time of the order of 0.5 kg (C) m(-2) with manageable sample sizes even using simple random sampling (i.e., about 50 samples per sampling point). More efficient strategies will reveal even smaller differences. On disturbed forest sites (ploughed, windthrow) this is no longer possible (required sample sizes are much larger than 100). Soils developed on coarse aeolian sediments (sand dunes), or where buried logs or harvest residues of the previous rotation are present, can also exhibit large spatial variability in soil carbon. Generally, carbon-rich soils will always require larger numbers of samples. On these sites, simple random sampling is unlikely to be the preferred method, because of its inherent inefficiency. More sophisticated approaches, such as paired re-sampling inside relatively small plots (see, for example, Ellert et al., 2001) are likely to reduce sample size significantly and lead to detection of smaller differences in carbon stocks over time. However, it remains to be shown that at these sites the application of efficient sampling designs will result in the detection of differences relevant for the objectives of the Kyoto Protocol (cf., Conant et al., 2003). Finally, it should also be noted that, compared to the accuracy with which changes in atmospheric carbon content can be detected (less than 1 p.p.m. CO2), changes in soil carbon stocks are very uncertain. A release of 0.5 kg (C) from 1 m2 of soil surface is equivalent to an increase in CO, concentration of about 125 p.p.m. in the air column above the same area.</p>","PeriodicalId":87484,"journal":{"name":"SEB experimental biology series","volume":" ","pages":"235-49"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26831732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Högberg, A. Nordgren, M. Högberg, M. Ottosson‐Löfvenius, Bhupinderpal-Singh, P. Olsson, S. Linder
{"title":"Fractional contributions by autotrophic and heterotrophic respiration to soil-surface CO2 efflux in Boreal forests.","authors":"P. Högberg, A. Nordgren, M. Högberg, M. Ottosson‐Löfvenius, Bhupinderpal-Singh, P. Olsson, S. Linder","doi":"10.4324/9780203501344-12","DOIUrl":"https://doi.org/10.4324/9780203501344-12","url":null,"abstract":"Soil-surface CO2 efflux ('soil respiration') accounts for roughly two-thirds of forest ecosystem respiration, and can be divided into heterotrophic and autotrophic components. Conventionally, the latter is defined as respiration by plant roots. In Boreal forests, however, fine roots of trees are invariably covered by ectomycorrhizal fungi, which by definition are heterotrophs, but like the roots, receive sugars derived from photosynthesis. There is also a significant leaching of labile carbon compounds from the ectomycorrhizal roots. It is, therefore, more meaningful in the context of carbon balance studies to include mycorrhizal fungi and other mycorrhizosphere organisms, dependent on the direct flux of labile carbon from photosynthesis, in the autotrophic component. Hence, heterotrophic activity becomes reserved for the decomposition of more complex organic molecules in litter and other forms of soil organic matter. In reality, the complex situation is perhaps best described as a continuum from strict autotrophy to strict heterotrophy. As a result of this, and associated methodological problems, estimates of the contribution of autotrophic respiration to total soil respiration have been highly variable. Based on recent stand-scale tree girdling experiments we have estimated that autotrophic respiration in boreal forest accounts for up to 50-65% of soil respiration during the snow-free part of the year. Girdling experiments and studies of the delta(13)C of the soil CO2 efflux show that there is a lag of a few days between the carbon uptake by photosynthesis and the release by autotrophic soil respiration of the assimilated carbon. In contrast, estimates of 'bomb 14C' and other approaches have suggested that it takes years to decades between carbon uptake via photosynthesis and the bulk of soil heterotrophic activity. Temperature is normally used as a driver in models of soil processes and it is often assumed that autotrophic soil activity is more sensitive to temperature than is heterotrophic activity, but this is questionable. It is inherently difficult to make a precise separation of autotrophic and heterotrophic respiration from soils. The partitioning between these two components is highly variable in space and time, and taxonomic autotrophs and heterotrophs may perform the function of the other group to some degree. Care should be taken to disturb as little as possible the delicate plant-microbe-soil system, and this speaks for non-intrusive isotopic methods. There are, however, problems in modelling the flux of isotopes through this complex system. Girdling of tree stands is a very robust alternative approach to make the distinction between autotrophic and heterotrophic activities, but ultimately kills the trees and cannot, therefore, always be used. A further development would be to block the phloem sugar transport reversibly. We propose that thus assumption needs further critical testing.","PeriodicalId":87484,"journal":{"name":"SEB experimental biology series","volume":"1 1","pages":"251-67"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70588411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. A. Black, D. Gaumont-Guay, R. Jassal, B. Amiro, P. Jarvis, S. Gower, F. Kelliher, A. Dunn, S. Wofsy
{"title":"Measurement of CO2 exchange between Boreal forest and the atmosphere.","authors":"T. A. Black, D. Gaumont-Guay, R. Jassal, B. Amiro, P. Jarvis, S. Gower, F. Kelliher, A. Dunn, S. Wofsy","doi":"10.4324/9780203501344-8","DOIUrl":"https://doi.org/10.4324/9780203501344-8","url":null,"abstract":"The Boreal forest is the world's second largest forested biome occupying the circumpolar region between 50 degrees N and 70 degrees N. This heterogeneous biome stores about 25% of all terrestrial carbon. We have reviewed EC measurements of CO2 exchange between the atmosphere and Boreal forests, and assessed progress in understanding the controlling processes. We have assessed net ecosystem productivity, the net balance between net primary productivity and heterotrophic respiration, measured using the EC method, for 38 Boreal forest sites. Gross ecosystem productivity has been estimated by adding day-time EC-measured CO2 fluxes to respiration estimated from night-time relationships between respiration and temperature. Maximum midday values of gross ecosystem productivity vary from 33 pmol m(-2) s(-1) for aspen to 6 micromol m(-2) s(-1) for larch stands. Long-term EC flux measurements, ongoing at nine Boreal sites, have shown the strong impact of spring weather and growing season water balance on annual net ecosystem productivity. Estimation of net biome production, incorporating the effects of disturbance resulting from forest fires and logging, has progressed significantly in recent years. After disturbance, summer measurements in Boreal chronosequences suggest that it takes about 10 years before growing season carbon uptake offsets the decomposition emissions. Small-scale exchange rate measurements using chambers and manipulative experiments such as stem girdling and soil heating help to understand the processes and mechanisms playing major roles in the carbon balance of terrestrial ecosystems. Aircraft EC flux measurements, convective boundary layer carbon budgets, and (13)C/12C changes in the atmosphere play an important role in validating estimates of regional carbon exchange based on scaled up EC measurements. Atmospheric inverse models are an important approach to studying regional and global carbon balance but need further improvement to yield reliable quantitative results.","PeriodicalId":87484,"journal":{"name":"SEB experimental biology series","volume":"1 1","pages":"151-85"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70588804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tuomas Laurila, Mika Aurela, Annalea Lohila, Juha-Pekka Tuovinen
{"title":"Trace gas and CO2 contributions of northern peatlands to global warming potential.","authors":"Tuomas Laurila, Mika Aurela, Annalea Lohila, Juha-Pekka Tuovinen","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":87484,"journal":{"name":"SEB experimental biology series","volume":" ","pages":"269-92"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26831734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rainer Brumme, Louis V Verchot, Pertti J Martikainen, Christopher S Potter
{"title":"Contribution of trace gases nitrous oxide (N2O) and methane (CH4) to the atmospheric warming balance of forest biomes.","authors":"Rainer Brumme, Louis V Verchot, Pertti J Martikainen, Christopher S Potter","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":87484,"journal":{"name":"SEB experimental biology series","volume":" ","pages":"293-317"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26831735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Carbon sequestration in European croplands.","authors":"Pete Smith, Pete Falloon","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Marrakech Accords allow biospheric carbon sinks and sources to be included in attempts to meet emission reduction targets for the first commitment period of the Kyoto Protocol. Forest management, cropland management, grazing land management, and re-vegetation are allowable activities under Article 3.4 of the Kyoto Protocol. Soil carbon sinks (and sources) can, therefore, be included under these activities. Croplands are estimated to be the largest biospheric source of carbon lost to the atmosphere in Europe each year, but the cropland estimate is the most uncertain among all land-use types. It is estimated that European croplands (for Europe as far east as the Urals) lose 300 Tg (C) per year, with the mean figure for the European Union estimated to be 78 Tg (C) per year (with one SD=37). National estimates for EU countries are of a similar order of magnitude on a per-area basis. There is significant potential within Europe to decrease the flux of carbon to the atmosphere from cropland, and for cropland management to sequester soil carbon, relative to the amount of carbon stored in cropland soils at present. The biological potential for carbon storage in European (EU 15) cropland is of the order of 90-120 Tg (C) per year, with a range of options available that include reduced and zero tillage, set-aside, perennial crops, deep rooting crops, more efficient use of organic amendments (animal manure, sewage sludge, cereal straw, compost), improved rotations, irrigation, bioenergy crops, extensification, organic farming, and conversion of arable land to grassland or woodland. The sequestration potential, considering only constraints on land use, amounts of raw materials and available land, is up to 45 Tg (C) per year. The realistic potential and the conservative achievable potentials may be considerably lower than the biological potential because of socioeconomic and other constraints, with a realistically achievable potential estimated to be about 20% of the biological potential. As with other carbon sequestration options, potential impacts of non-CO, trace gases also need to be factored in. If carbon sequestration in croplands is to be used in helping to meet emission reduction targets for the first commitment period of the Kyoto Protocol, the changes in soil carbon must be measurable and verifiable. Changes in soil carbon can be difficult to measure over a 5-year commitment period, and this has implications for Kyoto accounting and verification. Currently, most countries can hope to achieve only a low level of verifiability during the first commitment period, whereas those with the best-developed national carbon accounting systems will be able to deliver an intermediate level of verifiability. Very stringent definitions of verifiability would require verification that would be prohibitively expensive for any country. There is considerable potential in European croplands to reduce carbon fluxes to the atmosphere and to sequester carbon iri ","PeriodicalId":87484,"journal":{"name":"SEB experimental biology series","volume":" ","pages":"47-55"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26831763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}