Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology最新文献_第4页

Expression inhibition of multidrug resistance-associated protein (MRP1) by multi-ribozyme expression system in HEK293 cells. 多核酶表达系统抑制HEK293细胞中多药耐药相关蛋白(MRP1)的表达

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-02-01

Sheng Li Tian, Gui Yun Liu, Shuo Zheng, Hui Qing Liang, Shi De Liu, Jian Hua Zhang

{"title":"Expression inhibition of multidrug resistance-associated protein (MRP1) by multi-ribozyme expression system in HEK293 cells.","authors":"Sheng Li Tian, Gui Yun Liu, Shuo Zheng, Hui Qing Liang, Shi De Liu, Jian Hua Zhang","doi":"","DOIUrl":"","url":null,"abstract":"To study application of multi-ribozyme expression system on expression inhibition of multidrug resistance-associated protein (MRP1) in HEK293 cells, the multi-ribozyme expression system containing 20 cis-acting ribozymes for self-cleavage and 10 trans-acting ribozymes for targeting to MRP1 gene specific site were constructed. HEK293 cells cotransfected multi-ribozyme expression system with MRP1 target gene or full length of MRP1 gene respectively were analyzed by RT-PCR, Western blot analysis and MTT assay. The results showed that multi-ribozyme systems were able to dramatically decrease fluorescent fusion protein expression in HEK293 cells. RT-PCR analysis indicated that the extent of MRP1 target mRNA decrease was correlated with the number of trans-acting ribozyme contained in multi-ribozyme expression system. Similar changes have been observed from Western blot. MTT assay showed that multi-ribozyme systems were able to reverse MDR generated by MRP1 gene in HEK cells. These results suggested that inhibitory effects of multiple copies of ribozymes contained in the system were better than that of single ribozyme contained. Therefore, this strategy could be used in treatment of tumor or other diseases via gene therapy.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 1","pages":"43-52"},"PeriodicalIF":0.0,"publicationDate":"2009-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28061672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Hydrogen peroxide is involved in regulation of tobacco leaf senescence]. [过氧化氢参与调节烟草叶片的衰老】。］

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-02-01

Li Jiang, Xiao Wei Kong, Shu Qing Cao, Rong Xian Zhang

{"title":"[Hydrogen peroxide is involved in regulation of tobacco leaf senescence].","authors":"Li Jiang, Xiao Wei Kong, Shu Qing Cao, Rong Xian Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Effects of various exogenous hydrogen peroxide (H2O2) concentrations on leaf senescence of tobacco (Nicotiana tabacum L.) were studied. The results showed that 0.1 and 1 mmol/L H2O2 had little effects on the leaf senescence of tobacco, the contents of chlorophyll and soluble protein were decreased in varying degrees by 10, 100 mmol/L H2O2 treatment, the decreased chlorophyll and soluble protein contents were significantly correlated with increased endogenous H2O2 content. SDS-PAGE of soluble protein and localized staining of H2O2 showed consistency with respective content. Chloroplast ultrastructure were observed in the mesophyll cells of tobacco and there was a little change by 10 mmol/L H2O2 treatment, and a remarkable breakdown of granal stacks and disorganized thylakoid membranes were revealed by 100, 200 mmol/L H2O2 treatment. RT-PCR analysis showed the expression level of senescence associated genes SAG12 was low by 10mmol/L H2O2 treatment and increased by 100 mmol/L H2O2 treatment. These results strongly suggested the tobacco leaf senescence was induced by 10 mmol/L H2O2 and accelerated the progression by high concentration (100, 200 mmol/L) H2O2 stress.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 1","pages":"82-8"},"PeriodicalIF":0.0,"publicationDate":"2009-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28061585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Localization and dynamic change of saponin in root tuber of cultivated Pseudostellaria heterophylla]. [栽培太子参块根中皂苷的定位及动态变化]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2009-02-01

Hua Sheng Peng, Wen Zhe Liu, Zheng Hai Hu, Ling Zhang

{"title":"[Localization and dynamic change of saponin in root tuber of cultivated Pseudostellaria heterophylla].","authors":"Hua Sheng Peng, Wen Zhe Liu, Zheng Hai Hu, Ling Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Anatomical, histochemical and phytochemical methods were used to investigate the structure, the localization and content changes of total saponin of the root tuber of Pseudostellaria heterophylla on different developmental stages. Results showed that the primary structure and secondary structure of P. heterophylla adventitious root were similar to that of other herbaceous dicotyledons. 80% of mature root tuber were secondary xylem which consisted of most parenchyma cells and less vessels. The secondary phloem were composed of parenchyma cells too. The results of histochemical methods showed that saponin distributed in pericycle and parenchyma cells of primary phloem in the primary structure of root. In secondary structure and mature root tuber, saponin distributed in periderm and secondary vascular structure except cork layer and vessel. Besides, the colors of the secondary phloem were darker. The results of phytochemical test showed that the content of saponin in skin areas was higher than that in xylems in February and July. The result was consistent with that of histochemistry. The content of saponin in the head of the root was higher than that in the end of the root which was in turn higher than that in the middle part of the root. In the developmental process of root tuber, the content of saponin showed a dynamic tendency of high-low-high. This characteristics were related to the development of root system.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"42 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2009-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28061694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Genetic analysis of agronomic and physiological traits in rice under salt stress]. [盐胁迫下水稻农艺生理性状遗传分析]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-08-01

Jing Jiang, Wei Dong Yu, Jie Yun Zhuang, Ye Yang Fan, Bo Shen

引用次数: 0

[Identification and analysis of asymmetric somatic hybrids between B. oleracea and B. nigra]. [甘蓝与黑螺旋藻不对称体细胞杂种的鉴定与分析]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-08-01

Li Zhang, Hong Zhao, Fan Liu

{"title":"[Identification and analysis of asymmetric somatic hybrids between B. oleracea and B. nigra].","authors":"Li Zhang, Hong Zhao, Fan Liu","doi":"","DOIUrl":"","url":null,"abstract":"Asymmetric interspecific somatic hybrids between Brassica oleracea var. botrytis and B. nigra were produced by PEG-induced fusion of protoplasts radiated by different doses of UV. B. nigra, genotype St.461, has resistance to black rot, black leg and clubroot diseases which are popular in cabbage production. The regenerated plants were analyzed by several means including morphology observation, relative DNA content measurement by flow cytometry (FCM), chromosome counting, DNA molecular marker. Morphology observation indicated that the regenerated plants varied in morphology from intermediate type to cauliflower type. DNA contents of 20% regenerated plants were less than the sum of donor and recipient. Number of chromosomes in 23% of the regenerated plants were less than the sum of both parents. DNA molecular marker analysis (SRAP) demonstrated that the genetic information from the recipient parent was retained more or less complete and intact in the hybrids, while specific amplification bands of donor genome were lost in the hybrids from 20% to 97.77% . Furthermore, black-rot resistance test was performed in twenty-two regenerated plants, and seventeen of which showed good resistance against Xamthomonas campestris pv campestris. That gave the elementary proof of transferring of alien pathogen resistant genes from wild B. nigra to B. oleracea via UV mediated asymmetrical somatic hybridization. In conclusion, UV irradiation induced production of asymmetric hybrids between cauliflower and black mustard. Chromosome elimination or a limited introduction of donor chromosomes occurred in most of the hybrids, however, the degree of elimination was independent on UV doses.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"41 4","pages":"265-74"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37436440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of cilostazol on expression of thrombospondin-1 in diabetic retinopathy. 西洛他唑对糖尿病视网膜病变血小板反应蛋白-1表达的影响。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-08-01

Jian Yong Wang, Yang Shun Gu, Li Ping Yan, Xiao Dong Teng, Xiao Ming Zhang

{"title":"The effect of cilostazol on expression of thrombospondin-1 in diabetic retinopathy.","authors":"Jian Yong Wang, Yang Shun Gu, Li Ping Yan, Xiao Dong Teng, Xiao Ming Zhang","doi":"","DOIUrl":"","url":null,"abstract":"To explore the effect of cilostazol in the pathophysiology of diabetic retinopathy and its mechanism, we intraperitoneal injection streptozotocin (STZ) to induce rats diabetic model to study the alteration of the thrombospondin-1 (TSP-1) in the retina of diabetic rats, cilostazol treatment diabetic rats and normal rats by immunohistochemistry, real-time quantitative reverse transcription-polymerase chain reaction. The weight, blood sugar and urine sugar were also measured before and after model induction of these three groups. The data of weight, blood sugar and urine sugar indicated no significant difference in these three groups before diabetes induction. Four weeks after the injection of STZ, the weight, blood sugar and urine sugar had significant difference among these three groups (P < 0.01). When compared with the normal retina, TSP-1 expression was increased in the diabetic rat's retina, as shown by increased optical density and immunohistochemistry positive cell number but this was not serious in cilostazol treatment rats (P < 0.01). Our study confirmed that up-regulation of TSP-1 expression in retina of streptozotocin-induced diabetic rats under hyperglycemia condition and cilostazol treatment could prevent TSP-1 overexpression. This indicates a protective way in the pathophysiology of diabetic retinopathy.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"41 4","pages":"301-8"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37436444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and identification of the AFLP markers linked to a new powdery mildew resistance gene in wheat cultivar Brock. 小麦品种布洛克抗白粉病新基因AFLP标记的筛选与鉴定。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-08-01

Yi Wang, Jie Zhu, Hong Mei Zhao, Ding Han Lei, Zhen Ying Wang, Yong Kang Peng, Chao Jie Xie, Qi Xin Sun, Zhi Yong Liu, Zuo Min Yang

{"title":"Screening and identification of the AFLP markers linked to a new powdery mildew resistance gene in wheat cultivar Brock.","authors":"Yi Wang, Jie Zhu, Hong Mei Zhao, Ding Han Lei, Zhen Ying Wang, Yong Kang Peng, Chao Jie Xie, Qi Xin Sun, Zhi Yong Liu, Zuo Min Yang","doi":"","DOIUrl":"","url":null,"abstract":"Wheat cultivar Jing 411 which is susceptible to powdery mildew, wheat cultivar Brock and near isogenic lines (NILs) of Jing411, which are resistant to powdery mildew were analysized for polymorphisms using 225 pairs of AFLP primers Only two pairs of primers Pst+GAC/Mse+ TCT (P1) and Pst+AGC/Mse+ACC (P2) stably produced polymorphic bands between the resistant and susceptible plants. Two specific fragments were obtained. By cloning and sequencing these two specific fragments, it was showed that the specific fragment amplified by primer P1 had 268bp, and the fragment amplified by P2 had 227bp. They were named AFLP marker P1(268) and P2(227) respectively. Linkage analysis of these two markers revealed that the polymorphism existed in a 106 F2 segregating population. These two markers closely linked to a powdery mildew resistance gene in wheat cultivar Brock, linkage distance were 3.6 and 1.9 cM respectively. These two markers will be useful for marker-assisted selection and gene pyramiding in wheat resistance breeding.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"41 4","pages":"294-300"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37436443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Cytological observation of anther abortion and starch distribution of a cytoplasm male sterile pepper (Capsicum annum L.)]. [辣椒雄性不育细胞质花药败育及淀粉分布的细胞学观察]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-08-01

Yi Lan Qiu, Zhu Li Liu, Hong Li, Song Chen, Liang Bi Chen, Hui Qiao Tian

{"title":"[Cytological observation of anther abortion and starch distribution of a cytoplasm male sterile pepper (Capsicum annum L.)].","authors":"Yi Lan Qiu, Zhu Li Liu, Hong Li, Song Chen, Liang Bi Chen, Hui Qiao Tian","doi":"","DOIUrl":"","url":null,"abstract":"A cytoplasm male sterile pepper (Capsicum annum L.) was examined using cytochemical method to study its pollen abortion. Thick sections of both anthers of male sterility line 8214A and its maintainer 8214B at different stages were stained using Periodic Acid-Schiff's (PAS) reaction to detect starch distribution. Anther structure and starch distribution in both anthers of male sterility and maintainer line were similar before the meiosis of microspore mother cells. After meiosis, the size of tapetal cells of fertile anthers of maintainer line increased and became high vacuolation. Abundant small starches appeared in the connective cells from tetrad stage to early stage of microspore development. At the late stage of microspore, the tapetal cells began to degenerate and the starches in the connective cells became large. Bi-cellular pollen synthesized starches after the large vacuole of vegetative cell disappeared, and abundant starches were stored in the mature pollen. In the anthers of male sterile line, meiosis of microspore mother could occurred and the tetrads could be formed in the locule, but the tetrads were extruded together because the locule could not enlarge its space. Finally, the tetrad microspores degenerated. The development of vascular tissue of the sterile anthers was normal and abundant starches were stored in the connective cells, which suggested that the function of plant transporting polysaccharide into anther was normal but tapetum could not transport the polysaccharide into locule. According to our result, the pollen abortion occurred in the tetrad stage and the abnormal development of tapetal cells might be the reason which induced tetrad microspore abortion in this male sterile pepper.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"41 4","pages":"283-93"},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37436442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Construction and identification of the eukaryotic expression vector pIRES2-EGFP-IL-1ra-Fcepsilon]. 真核表达载体pIRES2-EGFP-IL-1ra-Fcepsilon的构建与鉴定

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-08-01

Zhong Cheng Liu, Yuan Yuan Wang, Min Ji Zou, Jia Xi Wang, Dong Gang Xu

引用次数: 0

Chromosomal behavior during the microsporogenesis of a trigeneric hybrid involving triticum, secale and leymus. 小麦、鳞片和羊草三基因杂交种小孢子发生过程中的染色体行为。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-08-01

Fang He, Hai Yan Liu, Hong Gang Wang, Xing Feng Li

引用次数: 0