Basic & Clinical Cancer Research最新文献

{"title":"Silibinin Inhibits TGF-β-induced MMP-2 and MMP-9 Through Smad Signaling Pathway in Colorectal Cancer HT-29 Cells","authors":"Z. Zare, Tina Nayerpour dizaj, Armaghan Lohrasbi, Zakieh Sadat Sheikhalishahi, Amirhooman Asadi, Mana Zakeri, F. Hosseinabadi, Omid Abazari, M. Abbasi, Parisa Khanicheragh","doi":"10.18502/BCCR.V12I2.5752","DOIUrl":"https://doi.org/10.18502/BCCR.V12I2.5752","url":null,"abstract":"Background: Metastasis of cancer cells is the primary responsible for death in patients with colorectal cancer (CRC). Transforming growth factor-β (TGF-β)-induced matrix metalloproteinases (MMPs) are essential for the metastasis process. Silibinin is a natural compound extracted from the Silybum marianum that exhibits anti-neoplastic activity in cancer cell lines. In this study, we evaluated the effects of silibinin on MMP-2 and MMP-9 induced by TGF-β in human HT-29 CRC cell line and the potential mechanism underlying the effects. Methods: The present in vitro study was done on the HT-29 cell line. The HT-29 cell line was cultured in RPMI1640 and exposed to TGFβ (5 ng/ml) in the absence and presence of different concentrations of silibinin (10, 25, 50, and 100 μM). The effect of silibinin on HT-29 cell viability was measured with the MTT assay. A real-time polymerase chain reaction (Real-Time PCR) determined the relative mRNA expression of MMP-2 and MMP-9. Western blotting was employed to examine MMP-2 and MMP 9 protein expression and Smad2 phosphorylation. Results: Silibinin inhibits cell viability of HT-29 cell line at 24 hours in a dose-dependent manner. TGF-β increased the mRNA and protein expression of MMP-2, MMP-9, and phosphorylated Smad2 compared to controls. Pharmacological inhibition with silibinin markedly blocked TGF-β–induced MMP-2 and MMP-9 mRNA and protein expression and Smad2 phosphorylation. Conclusion: Silibinin decreased the cell viability of HT-29 cancer cells in a dose-dependent manner. Silibinin also inhibited TGF-β-stimulated MMP-2 and MMP-9 expression in HT-29 cells, possibly mediated with the Smad2 signaling pathway.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89305082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opium Carcinogenicity: A Systematic Review of Experimental Studies","authors":"Haniyeh Rafipour, E. Mohebbi, K. Zendehdel, S. Muhammadnejad, Paria Akbari, S. Hashemi, Kosar Nouri, F. Moradkhani, Tahereh Barati, S. Amanpour","doi":"10.18502/BCCR.V12I2.5756","DOIUrl":"https://doi.org/10.18502/BCCR.V12I2.5756","url":null,"abstract":"Several epidemiological studies have reported that regular use of opium can be associated with an increased risk of developing cancers, including oesophageal, laryngeal, bladder, lung, and gastric cancer. In this systematic review, we aimed at investigating whether experimental studies support this finding and, if yes, how opium consumption can cause cancer. Most of the articles that have studied opium or its derivatives have found it as a carcinogen. However, due to the complex composition, different forms, and various ways of opium use, further comprehensive experimental studies are required. Using modern genomic and epigenomic methods seems to help determine the molecular mechanisms underlying opium carcinogenicity.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79964111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of MRP1 and ABCG2 Gene Expression in Chronic Myeloid Leukemia (CML) Patients","authors":"Saeed Solali, Masoumeh Fardi, S. Almasi, M. Aliparasti","doi":"10.18502/BCCR.V12I2.5755","DOIUrl":"https://doi.org/10.18502/BCCR.V12I2.5755","url":null,"abstract":"Background: This study evaluated and compared the quantitative expression of multidrug resistance-associated protein 1 (MRP1) and ATP-binding cassette subfamily G member 2 (ABCG2), two Multidrug Resistance (MDR) related genes, in 30 CML patients and 27 normal subjects. \u0000Methods: Total RNA was isolated from peripheral blood mononuclear cells (MNCs) using the Trizol reagent. Then cDNAs were synthesized. Gene expression was quantified using Real-Time PCR System. The relative expression of target genes was calculated using the 2-ΔΔCt method. \u0000Results: High expression of MRP1 and ABCG2 mRNAs were detected in the patient group. Intra-group comparisons also revealed increased expression of ABCG2 in Accelerated Phase (AP)-Blastic Crisis (BC) patients compared to Chronic Phase (CP) patients. At the same time, the increased expression of MRP1 in AP-BC patients was not statistically significant. \u0000Conclusion: Considering the broad spectrum of ATP Binding Cassette (ABC) transporter superfamily substrates, they can play an essential role in cell fate determination. High expression of MRP1 and ABCG2 genes can result in the efflux of therapeutic agents and subsequent reduction in their intracellular concentration. This mechanism finally protects cells from the therapeutic effects of medications. On the other hand, these transporters can export growth factors out of the cell. Such exported molecules may have a growth-inducing effect on adjacent cells. These are the possible mechanisms for the participation of MRP1 and ABCG2 genes in conferring drug resistance to CML cells.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77490683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Piperine on MMP-9, VEGF, and E-cadherin Expression in Breast Cancer MCF-7 Cell Line","authors":"Z. Zare, Tina Nayerpour dizaj, Armaghan Lohrasbi, Zakieh Sadat Sheikhalishahi, Mohammad Panji, F. Hosseinabadi, Vajiheh Najafi, Omid Abazari, M. Abbasi, Parisa Khanicheragh","doi":"10.18502/BCCR.V12I3.5767","DOIUrl":"https://doi.org/10.18502/BCCR.V12I3.5767","url":null,"abstract":"Background: Vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), and E-cadherin play a vital role in the behavior of angiogenesis, metastasis, and invasion of breast tumor cells. Piperine, the main component of Piper Nigrum, has shown anti-cancer properties in various malignancies. This Study investigates the potential effect of piperine on MMP-9, E-cadherin, and VEGF expression in breast cancer MCF-7 cell line. \u0000Methods: MTT assay was applied to assess the viability of MCF-7 cells. The mRNA levels of MMP-9, VEGF, and E-cadherin were assayed by qRT-PCR. Western blot was performed to identify the protein level of MMP-9. \u0000Results: MTT assay results showed that piperine treatment (5, 10, 25, 50, 75, and 100 μM) for 24 hours effectively inhibited cell viability of MCF-7 cells as compared with the control group. Furthermore, the gene expression of VEGF, MMP-9, and E-cadherin was dose-dependently suppressed by piperine treatment (5, 10 and 25 μM) (P<0.05; P<0.01). The results also indicated that piperine (5, 10, and 25 μM) significantly suppressed MMP-9 protein expression after 24 hours of piperine treatment (P<0.01). \u0000Conclusion: These results suggest that piperine may prevent angiogenesis, migration, and invasion of MCF-7 cells by suppressing MMP-9 and VEGF, and by inducing E-cadherin expression. Hence, it may be a suitable candidate for designing new drugs in cancer therapy.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87411784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lesson learned from a Pilot Project in Rudsar city in Gilan province for Breast Cancer Screening in Iran","authors":"K. Zendehdel","doi":"10.18502/BCCR.V12I2.5757","DOIUrl":"https://doi.org/10.18502/BCCR.V12I2.5757","url":null,"abstract":"The article's abstract is not available.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72450981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Major Risk Factors for Cervical Cancer in Northeast of Iran: Evidence from a Case-Control Study","authors":"F. H. Shandiz, Alireza Pasdar, F. Afzaljavan, Zohre Takalluo, M. Mofrad","doi":"10.18502/BCCR.V12I2.5754","DOIUrl":"https://doi.org/10.18502/BCCR.V12I2.5754","url":null,"abstract":"Background: Cervical cancer is a preventable cancer with various risk factors. In this study, we assessed different risk factors involved in invasive cervical cancer in the Northeast of Iran. Methods: In a case-control study, 99 patients with advanced cervical cancer were compared to 102 healthy, normal women. Cervical cancer risk factors were documented for these groups using a questionnaire and available medical notes. Univariate analysis was done for each risk factor, followed by multivariate regression analysis, to evaluate the most powerful risk factors after adjustment. Results: Multivariate model indicated that sexual transmitted diseases (STD) [p<0.001; OR=7.88, 95% CI (2.59-23.93)], age at first intercourse ≤16 [p=0.048; OR=6.22, 95% CI (1.06-36.51)] and age [p=0.001; OR= 1.11, 95% CI (1.04-1.18)] were independently significant risk factors for cervical cancer. Conclusion: According to this survey, the significant influence of major risk factors, including STD, age at first intercourse, and age itself, has been underlined. Moreover, increasing the social knowledge and educating people to prevent highrisk sexual behaviors, HPV testing, and routine use of HPV vaccine, which is nowadays regarded as a preventive measure in cervical cancer, may also be needed to be implemented in our prevention program.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76582226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water-pipe as a Risk Factor for Genital Warts? A Case Report","authors":"Faezeh Ghaemdoust, A. Nahvijou, F. Farzaneh","doi":"10.18502/BCCR.V11I3.5718","DOIUrl":"https://doi.org/10.18502/BCCR.V11I3.5718","url":null,"abstract":"Background: Human papillomavirus (HPV) infection is the most common sexually transmitted infection in the world. HPV infection can cause some types of cancer including female genital cancers (cervical cancer, vulvar) and male genital cancer as well as oropharyngeal cancers and genital warts. Cigarette smoking is a risk factor of cervical cancer or genital warts. Case presentation: This case report present a young woman who developed extensive genital warts a year after starting water-pipe smoking. These genital warts healed spontaneously after cessation of water-pipe smoking. Conclusion: The primary hypothesis that could be propounded, is that water-pipe smoke plays a role as an independent risk factor in developing genital warts, similar to cigarette smoke. In addition, water pipe smking may transmit different infections, including HPV infection through sharing the mouth tips of the water pipe between","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74043844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservation Options to Preserve Fertility in Female Cancer Patients: Available Clinical Practice and Investigational Strategies from the Oncology Guidelines Point of View","authors":"L. Mirzaeian, Haniyeh Rafipour, S. Hashemi, S. Zabihzadeh, S. Amanpour","doi":"10.18502/BCCR.V12I1.5726","DOIUrl":"https://doi.org/10.18502/BCCR.V12I1.5726","url":null,"abstract":"In recent years, advances in cancer treatment have improved the survival rate of cancer patients significantly. However, destructive damage to ovaries due to the therapies or cancer itself can cause different degrees of infertility in women of reproductive age that can affect their quality of life seriously. In this study, fertility cryopreservation options for female cancer patients in oncology guidelines were reviewed. Cryopreservation methods have a long history in reproductive biology and oncology. However, embryo and oocyte cryopreservation were the eligible restoration strategies in clinical oncology practice. Ovarian tissue cryopreservation (OTC) is the latest option recommended for fertility preservation in pre-pubertal and adult patients who cannot delay their treatment or in whom taking IVF hormones may have adverse effects on their cancer. Reports show that frozen-thawed ovarian tissue transplantation has led to more than 130 live births so far in patients, most of whom were cancer patients. Although OTC is indeed generally recognized as an investigational method, it is recommended in some important guidelines, such as ASCO 2018. Therefore, based on many clinical pieces of evidence , it is predicted that the investigational label will soon be removed, and OTC might be considered as one of the main fertility preservation options for female cancer patients in clinical oncology practice.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89927742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inutility of IMP3 Marker in Differentiating Hodgkin Lymphoma from Large Cell Lymphoma","authors":"A. Z. Mehrjerdi, M. Ahmadi","doi":"10.18502/BCCR.V12I1.5730","DOIUrl":"https://doi.org/10.18502/BCCR.V12I1.5730","url":null,"abstract":"Background: Hodgkin’s lymphoma is one of the most commonly diagnosed lym- phomas in Western society. Today Reed-Sternberg cells are identified by positive staining of several biomarkers. The IMP3 (insulin-like growth factor II m-RNA-bind- ing protein 3) marker is a member of the insulin-like growth factor II mRNA binding protein family that has been suggested as a diagnostic marker in some epithelial malignancies. In this study, we aimed to evaluate the expression profile of IMP3 in Hodgkin’s lymphoma patients and compare it with those with large cell lymphoma. \u0000Methods: In this study, patients diagnosed with Hodgkin’s lymphoma between 2016 and 2018 were recruited. For the control group, patients diagnosed with large cell lymphoma were chosen. Paraffin blocks were collected and cut by a microtome machine. Immunohistochemical staining was performed on the slides for the IMP3 marker, using the Envision method. The color intensity was divided into four groups, and data on age, gender, staining intensity, sampling rate, and staining pattern en- tered at the end of the checklists. The collected data were analyzed using SPSS 19 software. The paired t-test has was employed, and a significant statistical level of 0.05 was considered in all tests. \u0000Results: In this study, 145 patients in a wide range of 5 to 84 years (the mean age = 41 ± 17 years) were studied. Fifty-three patients were diagnosed with diffuse large B-cell lymphoma (36.6%), 4 cases (2.8%) with anaplastic large cell lymphoma and 88 cases with (60.7%) Hodgkin’s lymphoma. Among 145 patients in the current study, 143 patients (98.6%) were positive for IMP3. IMP3 was positive in all patients with Hodgkin’s lymphoma and anaplastic large cell lymphoma, and only 2 cases of diffuse large B-cell lymphoma were negative for this maker, in whom severe ne- crosis was noted. Consequently, there is not a vivid difference between Hodgkin’s lymphoma and non-Hodgkin’s lymphoma (p-value=0.153) \u0000Conclusion: The marker is positive for Hodgkin’s lymphoma with a negative back- ground and may be used as a supplementary marker along with CD15 and CD30 to detect neoplastic cells. However, it cannot help differentiate it from large cell lym- phomas because it is also positive for non-Hodgkin lymphomas.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87121370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Mathematical Model of Cancer Treatment by Radiotherapy","authors":"Seyed Mehdi Hosseini Motlagh, Faeze Lari Motefaker","doi":"10.18502/BCCR.V11I3.5716","DOIUrl":"https://doi.org/10.18502/BCCR.V11I3.5716","url":null,"abstract":"The aim of this study is achieve an analysis of the mathematical model governing radiotherapy as well as to achieve the concentration of healthy and cancerous cells to reduce the length of treatment and less damage to cancer treatment by this type of therapy. In order to obtain this, we used the latest mathematical radiotherapy model based on the Lotka-Volterra competitive equations and the Adomian decomposition method that is the one of the most advanced analytical solutions to solve differential equations to attain our goal. The calculation of the Adomian decomposition method was applied to the mathematical model governing radiotherapy, and then the concentration of healthy and cancerous cells was achieved with a very good approximation. Comparison of the behavior of healthy and cancerous cells concentrations based on experimental cases and the behavior of healthy and cancerous cells concentrations based on computations express the correctness of the work. ADM indicates the concentration of healthy and cancerous cells during the treatment stage and the no treatment stage can be effective in improving the modeling based on the competitive model of the Lotka-Volterra equations, which results in the reduction of the use of diagnostic devices, less radiation, the faster treatment process and decreasing the cost of treatment for patients and governments.","PeriodicalId":8706,"journal":{"name":"Basic & Clinical Cancer Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88412046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}