Avicenna journal of medical biotechnology最新文献_第9页

Evaluation of the Spatial Arrangement of Rabbit Hepatocytes Based on Voronoi Tessellation Following Exposure to Zinc Oxide Nanoparticles 基于 Voronoi Tessellation 评估暴露于氧化锌纳米颗粒后兔肝细胞的空间排列情况

Avicenna journal of medical biotechnology Pub Date : 2024-06-16 DOI: 10.18502/ajmb.v16i3.15742

Zia Moasses, Arefeh Aryan, Fakhroddin Mesbah, Esmaeil Mirzaei

{"title":"Evaluation of the Spatial Arrangement of Rabbit Hepatocytes Based on Voronoi Tessellation Following Exposure to Zinc Oxide Nanoparticles","authors":"Zia Moasses, Arefeh Aryan, Fakhroddin Mesbah, Esmaeil Mirzaei","doi":"10.18502/ajmb.v16i3.15742","DOIUrl":"https://doi.org/10.18502/ajmb.v16i3.15742","url":null,"abstract":"Background: Zinc oxide nanoparticles have been utilized in different fields over the last decades. These nanoparticles can pose significant risks to various organs such as the liver. This study aimed to evaluate the effects of zinc oxide nanoparticles on liver histology, serum biochemistry, and spatial arrangement of the hepatocytes in the female New Zealand white rabbit. \u0000Methods: The rabbits received 1, 5, and 10 mg/kg of the zinc oxide nanoparticles (ZnO NPs) intraperitoneally once every 3 days for 28 days. The serum levels of the aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and zinc were assessed 48 hr following the first administration. The histopathological changes and Voronoi tessellation were evaluated after the last administration. \u0000Results: Our findings showed that the ZnO NPs significantly increased the serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and zinc. The histopathological findings showed ballooning degeneration, and sinusoidal congestion in ZnO NPs administrated groups. The Voronoi tessellation diagrams also confirmed that ZnO NPs changed the regular spatial arrangement of hepatocytes to random and cluster patterns. \u0000Conclusion: In conclusion, the ZnO NPs alter the liver spatial arrangement and induce hepatic pathological changes that may affect liver function in rabbits","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"5 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141335664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lippia multiflora Leaves Extracts Enhance Cefotaxime Bactericidal Effects and Quench the Biofilm Formation in Methicillin-Resistant Staphylococcus aureus ATCC 43300 多花菩提叶提取物可增强头孢他啶的杀菌效果并抑制耐甲氧西林金黄色葡萄球菌 ATCC 43300 的生物膜形成

Avicenna journal of medical biotechnology Pub Date : 2024-06-16 DOI: 10.18502/ajmb.v16i3.15746

A. Rouamba, Djaouratou Badini, E. Compaoré, Vincent Ouedraogo, M. Kiendrebeogo

{"title":"Lippia multiflora Leaves Extracts Enhance Cefotaxime Bactericidal Effects and Quench the Biofilm Formation in Methicillin-Resistant Staphylococcus aureus ATCC 43300","authors":"A. Rouamba, Djaouratou Badini, E. Compaoré, Vincent Ouedraogo, M. Kiendrebeogo","doi":"10.18502/ajmb.v16i3.15746","DOIUrl":"https://doi.org/10.18502/ajmb.v16i3.15746","url":null,"abstract":"Background: The emergence of the multidrug-resistant bacteria strain has become a global world crisis. This study was designed to evaluate the antibiofilm and synergistic effects of Lippia multiflora leaf extracts on the activity of cefotaxime against the methicillin-resistant Staphylococcus aureus (S. aureus). \u0000Methods: The synergistic effect of methanol and dichloromethane extracts on the bactericidal activity of cefotaxime was determined by using the antibiotic susceptibility test on agar medium. The antibiofilm activity of the extracts was measured by using the crystal violet method. The antioxidant potential of the extracts was assessed by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric Reduction Activity Potential (FRAP) methods. The main secondary metabolites groups were analyzed by using different standard analytical tests. The total phenolics and total flavonoids were quantified spectrophotometrically. \u0000Results: The methanol extract (final concentration of 100 µg/ml) inhibited the formation of bacterial biofilm more than salicylic acid (p<0.05). All extracts combined with cefotaxime (20 µg and 200 µg) showed good synergistic bactericidal effect on S. aureus with inhibitory diameters of up to 40 mm. The methanol extract showed higher total phenolics (462.20±10.90 mg EAG/g) and total flavonoids (26.20±0.20 mg EQ/g) contents than the dichloromethane extract (96.70±1.70 mg EAG/g and 8.00±1.20 mg EQ/g). Moreover, the methanol extract showed a higher FRAP reducing power (353.6± 4.17 mmol EQ/g) than the dichloromethane extract (385.3±7.01 mmol EQ/g). Qualitative phytochemical analysis showed the presence of tannins, flavonoids, terpenes and sterols in both extracts. \u0000Conclusion: These data showed that L. multiflora leaves contain effective antibacterial phytomolecules for combating bacterial resistance.","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"10 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141336042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Recombinant Proteins for Treating Rheumatoid Arthritis 重组蛋白在治疗类风湿关节炎中的作用

Avicenna journal of medical biotechnology Pub Date : 2024-06-16 DOI: 10.18502/ajmb.v16i3.15739

Mahboubeh Soleimani SasaniSasani, Yeganeh Moradi

引用次数: 0

Poultry Gastrointestinal-derived Lactic Acid Bacteria (pGIT-d-LAB) Inhibit Multiple Antibiotics Resistance Bacterial and Fungal Pathogens 家禽胃肠道源性乳酸菌 (pGIT-d-LAB) 可抑制多种抗生素耐药性细菌和真菌病原体

Avicenna journal of medical biotechnology Pub Date : 2024-02-06 DOI: 10.18502/ajmb.v16i2.14862

Bolanle Adeniyi, Abimbola Adesuyi, F. Ayeni, T. Ogunbanwo, T. Agidigbi

{"title":"Poultry Gastrointestinal-derived Lactic Acid Bacteria (pGIT-d-LAB) Inhibit Multiple Antibiotics Resistance Bacterial and Fungal Pathogens","authors":"Bolanle Adeniyi, Abimbola Adesuyi, F. Ayeni, T. Ogunbanwo, T. Agidigbi","doi":"10.18502/ajmb.v16i2.14862","DOIUrl":"https://doi.org/10.18502/ajmb.v16i2.14862","url":null,"abstract":"Background: To develop a probiotic formulation for poultry feed, a few poultry gastrointestinal derived lactic acid bacteria (pGIT-d-LAB) were isolated from chicken intestinal specimens and in vitro experiment was performed to evaluate their efficacy as potential probiotic candidate. \u0000Methods: A total of 6 strains of LAB: Lactobacillus brevis (L. brevis), Lactobacillus acidophilus (L. acidophilus), Lactobacillus casei (L. casei), Pediococci spp, Lactobacillus fermentum (L. fermentum) and Lactobacillus plantarum (L. plantarum) were isolated and cultured for collection of Cell Free Supernatant (CFS). CFS collected was tested against pathogenic bacterial isolated from chicken feces as well as prevalent fungal pathogens, utilizing agar-well diffusion techniques. A preliminary investigation into the susceptibility of the pathogens to diverse antibiotics and antifungal drugs was conducted. Bacterial pathogens exhibiting resistance to a minimum of three classes of antibiotics were subsequently identified for pGIT-d-LAB CFS screening. \u0000Results: The observed results revealed that the CFS derived from the isolates exhibited varying degrees of growth inhibition against different pathogens. Among the tested pGIT-d-LAB isolates, L. acidophilus demonstrated the most prominent zone of inhibition, measuring 18 mm against Klebsiella pneumoniae ZTAC 1233. Notably, Citrobacter diversus ZTAC 1255 showed resistance to all tested pGIT-d-LAB. Quantification of the metabolites produced was performed, and peak production levels was determined. L. acidophilus produced the highest amount of lactic acid (1.789g/l), Pediococci spp. produced the highest amount of diacetyl and H202 (1.918g/l) (0.0025g/l) at 48 hr peak values respectively. \u0000Conclusion: The test isolates are potential probiotic candidates for controlling pathogens in poultry.","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"333 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139799118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CYP21A2 Gene Analysis in Southern Iranian CAH Patients and a Brief Review of the Mutation Spectrum 伊朗南部 CAH 患者的 CYP21A2 基因分析及突变谱简述

Avicenna journal of medical biotechnology Pub Date : 2024-02-06 DOI: 10.18502/ajmb.v16i2.14864

Danial Zangene, Hossein Moravej, H. Ilkhanipoor, A. Amirhakimi, Zhila Afshar, M. Entezam

{"title":"CYP21A2 Gene Analysis in Southern Iranian CAH Patients and a Brief Review of the Mutation Spectrum","authors":"Danial Zangene, Hossein Moravej, H. Ilkhanipoor, A. Amirhakimi, Zhila Afshar, M. Entezam","doi":"10.18502/ajmb.v16i2.14864","DOIUrl":"https://doi.org/10.18502/ajmb.v16i2.14864","url":null,"abstract":"Background: CYP21A2 gene mutations are responsible for more than 95% of Congenital Adrenal Hyperplasia (CAH) disorders with autosomal recessive inheritance. Most of these pathogenic mutations originate from the CYP21A1P, a neighboring pseudogene with 98% homology, due to unequal crossing over or gene conversion events. Mutation identification of the gene could be beneficial for accurate diagnosis and outcome prediction. \u0000Methods: Twelve unrelated patients with CAH diagnosis were recruited for genetic counseling. To ensure distinct amplification of the CYP21A2 gene rather than its pseudogene, the complete sequence of the gene was amplified through two overlapping fragments by specific primers. The entire sequences were screened by direct Sanger sequencing using new sequencing primers. \u0000Results: Only two pathogenic point mutations were identified. The c.293-13C>G, also known as In2G, and the c.955C>T mutations were found in 37.5 and 33.3% of alleles, respectively. One patient showed homozygous gene deletion. We also reviewed recent reports on CYP21A2 gene mutations in Iran. \u0000Conclusion: Evaluating the ethnicity-specific gene mutation data is significant for populations with diverse ethnic groups including the Iranian population. Although several common mutations have been reported as causative mutations among CAH patients, identifying only two common point mutations in Fars province would help prioritize exon sequencing and reduce the cost and time of genotyping.","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"27 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139800042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factor VIII as a Novel Biomarker for Diagnosis, Prognosis, and Therapy Prediction in Human Cancer and Other Disorders 因子 VIII 是人类癌症和其他疾病诊断、预后和治疗预测的新型生物标记物

Avicenna journal of medical biotechnology Pub Date : 2024-02-06 DOI: 10.18502/ajmb.v16i2.14857

Sheyda Khalilian, Zahra Mohajer, S. Ghafouri-Fard

引用次数: 0

The Protective Effect of N-acetylcysteine against Deltamethrin-Induced Hepatotoxicity in Mice N- 乙酰半胱氨酸对溴氰菊酯诱导的小鼠肝毒性的保护作用

Avicenna journal of medical biotechnology Pub Date : 2024-02-06 DOI: 10.18502/ajmb.v16i2.14859

Ali Ameri, Alireza Rahmati, Shadi Soroushfar, Mehdi Lalehzari, Tahereh Dehghani, Hamed Haghi-Aminjan, Jebreil Shamseddin, Mahmoud Omidi

{"title":"The Protective Effect of N-acetylcysteine against Deltamethrin-Induced Hepatotoxicity in Mice","authors":"Ali Ameri, Alireza Rahmati, Shadi Soroushfar, Mehdi Lalehzari, Tahereh Dehghani, Hamed Haghi-Aminjan, Jebreil Shamseddin, Mahmoud Omidi","doi":"10.18502/ajmb.v16i2.14859","DOIUrl":"https://doi.org/10.18502/ajmb.v16i2.14859","url":null,"abstract":"Background: Exposure to pesticides is of concern to public health officials worldwide. Deltamethrin is a synthetic pyrethroid pesticide which is widely used in agriculture and veterinary medicine. Deltamethrin poisoning is always one of the concerns in medical centers due to the deltamethrin induced hepatotoxicity. This study evaluated the hepatoprotective effects of N-acetylcysteine (NAC) against deltamethrin induced hepatotoxicity in mice \u0000Methods: A total of 40 BALB/c male mice were randomly divided into four groups; the first group was used as a control (0.5 ml normal saline); Groups 2-4 were treated with NAC [160 mg/kg Body Weight (BW)], deltamethrin (50 mg/kg BW), and NAC plus deltamethrin. At 1 and 24 hr after treatment, the animals were sacrificed and blood and liver samples were obtained for analysis and the liver/body ration, hepatic enzymes as Aspartate aminotransferase (AST), Alanine Transaminase (ALT), Alkaline phosphatase (ALP), Lactate dehydrogenase (LDH), Glutathione (GSH) content and Reactive Oxygen Species (ROS) level were measured. For comparison between more than two experimental groups, one-way ANOVA following Tukey test was used by SPSS software. \u0000Results: The deltamethrin significantly increased AST, ALT, ALP, and the level of ROS level at the end of 1 and 24 hr after treatment; while the LDH level and GSH content were decreased. Mice in the deltamethrin treated group had a higher liver/body weight ratio than in other treated groups after 24 hr. On the other hand, NAC in combination with deltamethrin significantly reduced the activities of AST, ALT, ALP, and increased GSH levels. \u0000Conclusion: This study demonstrated that NAC has a hepatoprotective role against deltamethrin-induced toxicity.","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"57 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139802361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Protective Effect of Crocin on Rat Bone Marrow Mesenchymal Stem Cells Exposed to Aluminum Chloride as an Endocrine Disruptor 氯霉素对暴露于内分泌干扰物氯化铝的大鼠骨髓间充质干细胞的保护作用

Avicenna journal of medical biotechnology Pub Date : 2024-02-06 DOI: 10.18502/ajmb.v16i2.14858

Elaheh Amini, Zahra Baharvand, A. Niknejad, Yasaman Tabari, Sahel Shemshadi

{"title":"The Protective Effect of Crocin on Rat Bone Marrow Mesenchymal Stem Cells Exposed to Aluminum Chloride as an Endocrine Disruptor","authors":"Elaheh Amini, Zahra Baharvand, A. Niknejad, Yasaman Tabari, Sahel Shemshadi","doi":"10.18502/ajmb.v16i2.14858","DOIUrl":"https://doi.org/10.18502/ajmb.v16i2.14858","url":null,"abstract":"Background: Mesenchymal Stem Cells (MSCs) have the ability to self-renew and proliferate which gives them healing properties in various tissues. Aluminium chloride (AlCl3) is a chemical compound with harmful effects on health; oxidative stress caused by Aluminium has been reported previously. Crocin, a major component of Crocus sativus (saffron), has antioxidant properties and has shown therapeutic potential. Researchers have been looking for ways to reduce the harmful effects of AlCl3. \u0000Methods: To investigate whether crocin can reduce AlCl3 cytotoxicity, rat Bone Marrow Mesenchymal Stem Cells (BM-MSCs) were isolated, cultured and divided into four experimental groups. The first group was the control, which was untreated cells. The second and third groups were treated with crocin (50, 100, 250, 500 µM) and AlCl3 (20, 25, 30 mM) for 24 hr. The fourth group was pre-treated with crocin (250, 500 µM) for 24 hr and then treated with AlCl3 (20 mM) overnight. Cytotoxicity was assessed using the MTT assay. Mineralization was evaluated by alizarin red staining. Sox-2 and E-cadherin expression were measured using real-time PCR. \u0000Results: The results showed that AlCl3 caused cytotoxicity on BM-MSCs and decreased the mRNA expression of Sox-2 and E-cadherin, which are important for the maintenance of self-renewal and proliferation of BM-MSCs. In contrast, crocin protected the self-renewal characteristic of BM-MSCs by increasing Sox-2 expression and also preserved the proliferative effects on BM-MSCs by upregulating E-cadherin expression (***p≤0.001). \u0000Conclusion: Overall, the study suggests that crocin can protect BM-MSCs from AlCl3-induced cytotoxicity by upregulate Sox-2 expression and E-cadherin expression. This suggests that crocin may be a potential therapeutic agent for the treatment of AlCl3-induced toxicity. \u0000 ","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"20 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139862126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Protective Effect of Crocin on Rat Bone Marrow Mesenchymal Stem Cells Exposed to Aluminum Chloride as an Endocrine Disruptor 氯霉素对暴露于内分泌干扰物氯化铝的大鼠骨髓间充质干细胞的保护作用

Avicenna journal of medical biotechnology Pub Date : 2024-02-06 DOI: 10.18502/ajmb.v16i2.14858

Elaheh Amini, Zahra Baharvand, A. Niknejad, Yasaman Tabari, Sahel Shemshadi

{"title":"The Protective Effect of Crocin on Rat Bone Marrow Mesenchymal Stem Cells Exposed to Aluminum Chloride as an Endocrine Disruptor","authors":"Elaheh Amini, Zahra Baharvand, A. Niknejad, Yasaman Tabari, Sahel Shemshadi","doi":"10.18502/ajmb.v16i2.14858","DOIUrl":"https://doi.org/10.18502/ajmb.v16i2.14858","url":null,"abstract":"Background: Mesenchymal Stem Cells (MSCs) have the ability to self-renew and proliferate which gives them healing properties in various tissues. Aluminium chloride (AlCl3) is a chemical compound with harmful effects on health; oxidative stress caused by Aluminium has been reported previously. Crocin, a major component of Crocus sativus (saffron), has antioxidant properties and has shown therapeutic potential. Researchers have been looking for ways to reduce the harmful effects of AlCl3. \u0000Methods: To investigate whether crocin can reduce AlCl3 cytotoxicity, rat Bone Marrow Mesenchymal Stem Cells (BM-MSCs) were isolated, cultured and divided into four experimental groups. The first group was the control, which was untreated cells. The second and third groups were treated with crocin (50, 100, 250, 500 µM) and AlCl3 (20, 25, 30 mM) for 24 hr. The fourth group was pre-treated with crocin (250, 500 µM) for 24 hr and then treated with AlCl3 (20 mM) overnight. Cytotoxicity was assessed using the MTT assay. Mineralization was evaluated by alizarin red staining. Sox-2 and E-cadherin expression were measured using real-time PCR. \u0000Results: The results showed that AlCl3 caused cytotoxicity on BM-MSCs and decreased the mRNA expression of Sox-2 and E-cadherin, which are important for the maintenance of self-renewal and proliferation of BM-MSCs. In contrast, crocin protected the self-renewal characteristic of BM-MSCs by increasing Sox-2 expression and also preserved the proliferative effects on BM-MSCs by upregulating E-cadherin expression (***p≤0.001). \u0000Conclusion: Overall, the study suggests that crocin can protect BM-MSCs from AlCl3-induced cytotoxicity by upregulate Sox-2 expression and E-cadherin expression. This suggests that crocin may be a potential therapeutic agent for the treatment of AlCl3-induced toxicity. \u0000 ","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"115 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139802058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple Determination of Bosentan in Plasma Samples by Reversed-Phase High-Performance Liquid Chromatography 用反相高效液相色谱法简单测定血浆样品中的波生坦

Avicenna journal of medical biotechnology Pub Date : 2024-02-06 DOI: 10.18502/ajmb.v16i2.14861

Naser Abbasi

{"title":"Simple Determination of Bosentan in Plasma Samples by Reversed-Phase High-Performance Liquid Chromatography","authors":"Naser Abbasi","doi":"10.18502/ajmb.v16i2.14861","DOIUrl":"https://doi.org/10.18502/ajmb.v16i2.14861","url":null,"abstract":"Background: In order to measure the plasma levels of Losartan and Bosentan, a sensitive Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) technique was developed. \u0000Methods: To compare bioavailability, the Area Under the Curve (AUC), peak plasma concentration (Cmax), and time to Cmax (Tmax) were employed. The standard curve (150-2400 ng/ml) was linear (R2=0.999), relative errors were between 2.4 to 10.05% and the coefficient of variation (CV%) ranged from 1.52 to 10.88. A single dosage (test and reference) was used for the in vivo investigation, which involved 16 healthy individuals. \u0000Results: The AUC0-48, AUC0-, Cmax, and Tmax of the test and reference had no statistically significant differences. The Cmax and 95% confidence intervals of the ratio of Cmax of the two formulations were 0.93-0.96 and 97.6-135%, respectively. \u0000Conclusion: Therefore, it was established that generic Bosentan was equivalent to Bosentan from Actelion and that both medications could be regarded as equally effective in clinical settings. The blood level of Bosentan could be measured using this straightforward procedure in all hospital laboratories. \u0000 ","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"463 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139860300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0