Avicenna journal of medical biotechnology最新文献

{"title":"<i>MGMT</i> Gene rs1625649 Polymorphism in Iranian Patients with Brain Glioblastoma: A Case Control Study.","authors":"Reyhaneh Safaei,&nbsp;Hanieh Mojtahedi,&nbsp;Sara Hanaei,&nbsp;Azadehsadat Razavi,&nbsp;Marzie Esmaeili,&nbsp;Maryam Sadr,&nbsp;Arezou Rezaei,&nbsp;Maryam Edalatfar,&nbsp;Hamidreza Khayat Kashani,&nbsp;Mohsen Sadeghi-Naini,&nbsp;Farzaneh Darbeheshti,&nbsp;Jaber Gharehdaghi,&nbsp;Mehdi Forouzesh,&nbsp;Abdolali Ebrahimi,&nbsp;Nima Rezaei","doi":"10.18502/ajmb.v15i1.11424","DOIUrl":"10.18502/ajmb.v15i1.11424","url":null,"abstract":"<p><p>Glioblastoma (GBM) is the most common and aggressive primary malignant brain tumor with poor prognosis and high potential of dispersion to other brain tissues in adult. Effective and modern choices of treatment including chemotherapy with alkylating agents marginally extend survival of GBM. However, alkylating agents can lead to highly harmful mismatch during DNA replication causing apoptosis and cell death. Accordingly, O6-Methylguanine-DNA methyltransferase (MGMT) removes alkyl adducts, thereby causing resistance to alkylating drugs. Single-Nucleotide Polymorphisms (SNPs) in MGMT promoter region may play a role in the regulation of MGMT expression and prediction of glioma development risk. In order to evaluate the clinical significance of rs1625649 SNP in the MGMT promoter region of glioblastoma, genomic DNA from a series of 54 patients with GBM and 50 healthy individuals in Iranian population were collected for tetra ARMS PCR amplification. None of the \"A\" or \"C\" alleles were associated with tumor occurrence, the \"AA\" genotype was more frequent in healthy subjects, and the \"AC\" genotype was 4.6 times more common in patients with GBM. The longest survival time was observed in the \"CC\" genotype; however, this difference was not statistically significant. On the other hand, homozygous rs1625649 (AA genotype) was significantly associated with a better survival than the cases with heterozygous rs1625649 (CA genotype) or wild type rs1625649 (CC genotype), predicting better response to temozolomide-based chemotherapy.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e2/30/AJMB-15-48.PMC9895979.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10735019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammation-Schizophrenia: A Bidirectional Causal Association Mediated by Cytokines.","authors":"Ahmad Shamabadi,&nbsp;Shahin Akhondzadeh","doi":"10.18502/ajmb.v15i1.11418","DOIUrl":"10.18502/ajmb.v15i1.11418","url":null,"abstract":"The Article Abstract is not available.","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/95/d4/AJMB-15-1.PMC9895981.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10735021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Biomarkers in Early Diagnosis of Acute Kidney Injury in Children.","authors":"Behnaz Bazargani,&nbsp;Mastaneh Moghtaderi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Acute Kidney Injury (AKI) is a common condition with a high risk of mortality and morbidity, so, early diagnosis and management of AKI is very important in clinical practice. Despite significant progress in the management of AKI, it still carries high morbidity and mortality. BUN and serum creatinine are not very sensitive nor specific for the diagnosis of AKI because they are affected by many renal and non-renal factors that are independent of kidney injury or kidney function and change significantly only after significant kidney injury and with a substantial time delay. Detection of biomarkers of AKI made predominantly by the injured kidney tissue are essential for the early diagnosis of AKI. An ideal biomarker should be one that could be easily measured, with no interference with other biologic variables, and be able to clarify early phases of kidney damage. The most common biomarkers studied are Neutrophil Gelatinase-Associated Lipocalin (NGAL), Interleukin-18 (IL-18), Kidney Injury Molecule-1 (KIM-1), Cystatin-C, L type Fatty Acid-Binding Protein (L-FABP), N-Acetyl-β-D Glucosaminidase (NAG), netrin-1, vanin-1, and Monocyte Chemoattractant Protein-1 (MCP-1) and calprotectin.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2d/72/AJMB-14-264.PMC9706243.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10335737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between <i>PTCH1</i> and <i>RAD54B</i> Single-Nucleotide Polymorphisms and Non-syndromic Orofacial Clefts in the Northeast Population of Iran.","authors":"Reza Morvaridi Farimani,&nbsp;Mohsen Azimi-Nezhad,&nbsp;Hamid Reza KhorramKhorshid,&nbsp;Asghar Ebadifar,&nbsp;Saba Tohidkhah,&nbsp;Zahra Jafarian,&nbsp;Koorosh Kamali,&nbsp;Zeinab Nazari,&nbsp;Reza Ebrahimzadeh-Vesal","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Non-Syndromic Cleft Lip with or without cleft Palate (NSCL/P) is a common developmental disorder of the head and neck with a multifactorial etiology. The current study aimed to evaluate the potential association of <i>PTCH1</i> (rs10512248) and <i>RAD54B</i> (rs12681366) polymorphisms with NSCL/P in the Northeast Iranian population.</p><p><strong>Methods: </strong>In the present study, blood samples were taken from 122 subjects with NSCL/P and 161 healthy controls. Polymerase Chain Reaction (PCR) followed by Restriction Fragment Length Polymorphism (RFLP) were used to conduct genotyping of single-nucleotide polymorphisms.</p><p><strong>Results: </strong>Although differences were observed between cases and controls in rs10512248 and rs12681366, our data did not support a significant association of these polymorphisms with NSCL/P in our population.</p><p><strong>Conclusion: </strong>Our findings suggest that polymorphisms of rs10512248 and rs12681366 may not be potential risk factors for NSCL/P in the Northeast Iranian population due to the multifactorial and multiethnicity characteristics of some genes.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/50/bf/AJMB-14-310.PMC9706251.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10335731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Materials Derived from Menstrual Blood Stem Cells Enhance the Quality of <i>In Vitro</i> Bovine Embryos.","authors":"Mohammad Sobhan Amini,&nbsp;Mohammad Mehdi Naderi,&nbsp;Abolfazl Shirazi,&nbsp;Mehdi Aminafshar,&nbsp;Sara Borjian Boroujeni,&nbsp;Mostafa Pournourali,&nbsp;Ali Malekpour","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Backgrounds: </strong>The aim of this study was to determine whether the addition of bioactive materials derived from Menstrual Blood Stem Cells (MenSCs) to the oocyte maturation medium may improve the quality of bovine embryos <i>in vitro</i>.</p><p><strong>Methods: </strong>MenSCs were collected from 6 healthy women (between 26 and 36 years old) and after 3 days of culture, their bioactive materials were frozen. The bovine Cumulus-Oocyte-Complexes (COCs) were aspirated from ovarian slaughterhouse and the oocytes with more than three layers of cumulus cells were cultured <i>in vitro</i> in media supplemented with (treatment) and without (control) 10% MenSCs' bioactive materials. After IVM/IVF, the presumptive zygotes were cultured for 8 days.</p><p><strong>Results: </strong>The blastocyst rate on day 8 in treatment group was higher than control (40.2±1.9 <i>vs</i>. 23±4.2.3, p=0.001). The ratio of Trophectoderm (TE) and Inner Cell Mass (ICM) (ICM/TE) cells was also greater in treatment group compared to control (30.3±2 <i>vs</i>. 14.9±1; p=0.001). The re-expansion of vitrified blastocysts, 24 hours after warming, in treatment group was higher than control (93.3±2.5 <i>vs</i>. 66.2±8.8; p=0.01). The expression of some genes related to pluripotency and implantation (<i>OCT4</i>, <i>CDX2</i>, and <i>IFNT</i>) were increased in treatment group compared to control (p<0/05).</p><p><strong>Conclusion: </strong>In conclusion, the addition of MenSCs' bioactive materials during <i>in vitro</i> maturation of bovine oocytes could improve the quantity and quality of bovine IVP embryos. Also, the expression of some genes associated with pluripotency and implantation in the blastocyst would be increased.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2b/98/AJMB-14-287.PMC9706245.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10335738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibodies Produced Toward Recombinant RBD and Nucleocapsid Neutralize SARS-COV-2.","authors":"Amir Rezaei,&nbsp;Shahram Nazarian,&nbsp;Hossein Samiei Abianeh,&nbsp;Emad Kordbacheh,&nbsp;Zahra Alizadeh,&nbsp;Seyed Latif Mousavi Gargari","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>The highly contagious SARS-COV-2 virus spread rapidly from China and formed a global pandemic. The virus has infected over 509 million people worldwide and killed about 6.32 million up to date. Up on invasion, the Receptor Binding Domain (RBD) of Spike protein plays a crucial role in the entry of the virus into the host cell. The virus N protein is another protein that has a critical role for genome packaging.</p><p><strong>Methods: </strong>As bioinformatics approaches, the cassette design, codon adaptation, and protein stability were investigated in this study. Synthetic genes of RBD and N were cloned separately in <i>pET28a</i> + expression vector. They were transferred into <i>Escherichia coli</i> (<i>E. coli</i>) BL21 DE3 host cell, and expression of recombinant proteins was induced with IPTG. The recombinant proteins were purified by column chromatography and approved by Western blotting. Animal immunization was performed with each of the recombinant proteins individually and in combination of the two. The antibody titer of the blood serum from control and immunized mice groups was determined by ELISA technique. Finally, the anti-spike neutralization test was performed.</p><p><strong>Results: </strong>The expression and purification of RBD protein were monitored on SDS-PAGE, two bands of about 28 and 45 <i>kDa</i> for RBD and N appeared on gel distinctly, which were further validated by Western blotting. According to ELISA results, related antibodies were traced to a dilution of 1/64000 in immunized sera. The neutralization test exhibited produced antibodies' potency to bind the virus proteins. Using SPSS software, statistical analysis was performed by Duncan's test and T-test.</p><p><strong>Conclusion: </strong>According to the present study, recombinant proteins, either RBD alone or in combination with N adequately stimulated the immune response, and the raised antibodies could neutralize the virus in <i>in vitro</i> test.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/83/3f/AJMB-14-270.PMC9706246.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9184074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial Transfer from Menstrual Blood Stromal/Stem Cells Promotes Survival of Cardiomyocytes Following Myocardial Infarction.","authors":"Mahmood Manshori,&nbsp;Somaieh Kazemnejad,&nbsp;Hannaneh Golshahi","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9b/d8/AJMB-14-321.PMC9706244.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10329876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of PLGA-Encapsulated Recombinant GroEL of <i>S. typhi</i> immune Responses Against Enterohaemorrhagic and Enteropathogenic <i>Escherichia coli</i>.","authors":"Milad Parvane,&nbsp;Shahram Nazarian,&nbsp;Emad Kordbache,&nbsp;Javad Fathi,&nbsp;Mohamad Ebrahim Minae,&nbsp;Mohammad Reza Ramezani","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Heat Shock Proteins (HSPs) elicit humoral and cellular immune responses. Due to their high sequence homology, they can be developed as a new immunogen for cross prophylactic and vaccination effects against infectious agents such as Enteropathogenic and Enterohemorrhagic <i>Escherichia coli</i> (EPEC and EHEC). This study aimed to evaluate the immunogenicity and cross-protective efficacy of rGroEL of <i>Salmonella typhi</i> (<i>S. typhi</i>) encapsulated in poly lactic-co-glycolic acid (PLGA) nanoparticles against EPEC and EHEC.</p><p><strong>Methods: </strong>Recombinant GroEL was expressed in <i>Escherichia coli</i> (<i>E. coli</i>) and purified using Ni-NTA affinity chromatography. The protein was encapsulated in PLGA by the double emulsion method, and the nanoparticles were characterized physicochemically. BALB/c mice were immunized, and the efficacy of the protein to elicit immune responses was assessed.</p><p><strong>Results: </strong>Over-expression in <i>E. coli</i> led to corresponding 64.5 <i>kDa</i> protein bands in Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Non-aggregated nanoparticles had a spherical shape with a mean diameter of 194.3±3 <i>nm</i> and encapsulation efficiency of 89.5±2.5%. Antibody isotyping revealed that GroEL immunization induced both IgG1 and IgG2a antibodies. Moreover, immunization of the mice with recombinant GroEL protein conferred 80 and 60% protection against lethal infections by EPEC and EHEC, respectively. Furthermore, organ burden studies revealed a significant reduction in infection in the immunized mice compared to the non-immunized ones. Passive immunization with anti-GroEL sera also protected 50% of the mice against the lethal doses of EHEC and EPEC strains.</p><p><strong>Conclusion: </strong>The findings indicated that immunization of the mice with recombinant GroEL of <i>S. typhi</i> elicited cross-protection against other bacterial infections. This represented the immense potential of GroEL to be developed as a single vaccine against multiple pathogens.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b6/7a/AJMB-14-294.PMC9706248.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10323507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Poly-N-isopropylacrylamide Microgels Containing Antibiofilm Substances on <i>Pseudomonas aeruginosa</i> Isolated from Chronic Wounds.","authors":"Akram Etemadinia,&nbsp;Amir Seyfoori,&nbsp;Abbas Rahimi Foroushani,&nbsp;Ramin Mazaheri Nezhad Fard,&nbsp;Ronak Bakhtiari","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Biofilm formation helps <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) survive in various environments. Microgels can be effective in treatment of bacterial infections. The major aim of this study was to investigate effects of poly-N-isopropylacrylamide microgels (PNIPAM) on <i>P. aeruginosa</i>.</p><p><strong>Methods: </strong>Totally, 100 <i>P. aeruginosa</i> strains were isolated from chronic wound infections. Quantitative assessments of biofilm formation and antibiotic susceptibility were carried out. Furthermore, <i>alg</i>D, <i>las</i>R, and <i>PA</i>2714 genes were amplified to investigate gene frequencies and expression rates.</p><p><strong>Results: </strong>Significant decreases were seen in <i>las</i>R expression in EDTA-treated samples. Significant decreases were observed in expression of <i>alg</i>D and <i>las</i>R treated with xylitol. Decreased expression of <i>PA</i>2714 was seen in samples treated with xylitol with no significance.</p><p><strong>Conclusion: </strong>The PNIPAM containing xylitol or EDTA could penetrate biofilms of <i>P. aeruginosa</i> and significantly decrease expression of <i>las</i>R and <i>alg</i>D. This can be a novel strategy in the management of chronic wounds.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b2/ca/AJMB-14-317.PMC9706250.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10335734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Antioxidant and Anti-gout Activities of <i>Citrullus colocynthis</i> loaded Fruit Silver nanoparticles with its Ethanolic extract.","authors":"Suganya Karunakaran,&nbsp;Rajeswary Hari","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>The biological synthesis of silver nanoparticles (AgNPs) using plant materials is a rapidly developing method with several alternative medical applications. This comparative study of ethanolic fruit extract of <i>Citrullus colocynthis</i> (<i>C. colocynthis</i>) (EFECC) and synthesized silver nanoparticles (CC-AgNPs) were carried out for antioxidants and anti-gout arthritic activities.</p><p><strong>Methods: </strong>The AgNPs were synthesized using <i>C. colocynthis</i> fruit and its characterization was done by UV-visible spectroscopy, TEM, XRD and FT-IR. The 90% ethanol was used for extract preparation. Antioxidant activity was analyzed by DPPH and the Hydrogen Peroxide (H₂O₂) method. <i>In vitro</i> anti-arthritic activity was tested by xanthine oxidase inhibition, protein denaturation and HRBC membrane stabilization assay.</p><p><strong>Results: </strong>The synthesized CC-AgNPs were confirmed by UV-vis spectroscopy and TEM images displayed spherical shapes with 10-45 <i>nm</i> size range. Furthermore, the functional groups and crystalline structure of CC-AgNPs were determined by FT-IR and XRD analysis. The biosynthesized CC-AgNPs exhibited an excellent free radical scavenging ability than EFECC. In anti-arthritic activity, the CC-AgNPs showed effective inhibition of xanthine oxidase production, protein denaturation, and damaged RBC membranes compared to EFECC.</p><p><strong>Conclusion: </strong>The antioxidant activities and <i>in vitro</i> anti-arthritic assays revealed that CC-AgNPs are better anti-gout agents than EFECC. This research suggested that biosynthesized silver nanoparticles from <i>C. colocynthis</i> fruit are an important target in the field of anti-gout drug discovery.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bf/bd/AJMB-14-303.PMC9706249.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10335735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}