Die Pharmazie. Beihefte最新文献_第7页

Long non-coding RNA HEIH promotes breast cancer development via negative modulation of microRNA-200b. 长链非编码RNA HEIH通过负调控microRNA-200b促进乳腺癌的发展。

Die Pharmazie. Beihefte Pub Date : 2019-08-01 DOI: 10.1691/ph.2019.9428

Jie Zhao, R. Meng, Q. Yao, Hui Wang, J. Niu, Y. Cui, Songlin Chen, Yin-Shan Bai

{"title":"Long non-coding RNA HEIH promotes breast cancer development via negative modulation of microRNA-200b.","authors":"Jie Zhao, R. Meng, Q. Yao, Hui Wang, J. Niu, Y. Cui, Songlin Chen, Yin-Shan Bai","doi":"10.1691/ph.2019.9428","DOIUrl":"https://doi.org/10.1691/ph.2019.9428","url":null,"abstract":"This study aimed to investigate the effects and regulatory mechanism of long non-coding RNA HEIH in the development of breast cancer. The expression of HEIH in breast tumor tissues and breast cancer cells was determined, followed by investigating the effects and regulatory mechanism of HEIH dysregulation on breast cancer cell viability, apoptosis, migration and invasion. The expression of HEIH was upregulated in breast cancer tissue samples and cell lines. Suppression of HEIH inhibited cell viability, promoted cell apoptosis, and decreased migration and invasion in MDA-MB-231 cells. Moreover, a negative relationship existed between HEIH and miR-200b, and HEIH regulated breast cancer development via regulating miR-200b. Pre-leukemia transcription factor 3 (PBX3) was verified as a functional target of miR-200b, and miR-200b regulated the malignant behaviors of breast cancer cells through targeting PBX3. Furthermore, suppression of HEIH inhibited the activation of Wnt/β-catenin pathway, which was remarkably reversed after suppression of HEIH and inhibition of miR-200b synchronously. Our results reveal that HEIH may contribute to breast cancer development via modulation of microRNA-200b/axis and inducing the activation of Wnt/β-catenin pathway. Further studies are still required to confirm our findings.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"43 1","pages":"471-476"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87446044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Effects of leptin on norepinephrine in acute ischemic stroke. 瘦素对急性缺血性脑卒中去甲肾上腺素的影响。

Die Pharmazie. Beihefte Pub Date : 2019-08-01 DOI: 10.1691/ph.2019.9379

Pang Tao, Z. Jing, Gao Shou-Hong, Piao Shu-juan, Jiao Jian-peng, Lu Wen-Quan, Chen Wan-sheng

引用次数: 6

Impact of a systematic education model for palliative care in cancer. 系统教育模式对癌症姑息治疗的影响。

Die Pharmazie. Beihefte Pub Date : 2019-08-01 DOI: 10.1691/ph.2019.9417

M. Uchida, M. Hada, M. Yamada, D. Inma, S. Ariyoshi, K. Aoki, S. Inoue, T. Shimazoe, K. Mitsuiki, T. Haraguchi

{"title":"Impact of a systematic education model for palliative care in cancer.","authors":"M. Uchida, M. Hada, M. Yamada, D. Inma, S. Ariyoshi, K. Aoki, S. Inoue, T. Shimazoe, K. Mitsuiki, T. Haraguchi","doi":"10.1691/ph.2019.9417","DOIUrl":"https://doi.org/10.1691/ph.2019.9417","url":null,"abstract":"In clinical practice, pharmacists are continually required to improve their knowledge and expertise; however, the postgraduate education system for professional development cannot be confidently stated to be well established. The establishment of a systematic and multifaceted educational curriculum should be useful to improve home care and pharmacists' contribution; therefore, we developed a curriculum in collaboration with the university faculty of pharmaceutical sciences, department of pharmacy in hospital, and the Fukuoka Pharmaceutical Association. Class topics were extracted from the \"Kanwa-Iryou-Yakugaku\", edited by the Japanese Society for Pharmaceutical Palliative Care and Sciences. The items are necessary to perform palliative care as a pharmacist. A class schedule of 6 days (24 classes in total) was formulated. Questionnaires on comprehension degree before and after each class were provided to the participants. Comprehension was assessed on a scale of 1 to 10, where \"I do not understand at all\" was 1 and \"I understand enough\" was 10. The average recovery rates of questionnaires from each class were 92.6 % and 88.9 % before and after class, respectively. The average number of participants who completely answered the questionnaire before and after class was 45.6; therefore, these data were analyzed. Comprehension degree on each topic had significantly increased after attendance of all classes (p < 0.01). The comprehension degree of participants of the medical science of palliative care did greatly improve. Consequently, it is clear that the standard education model constructed was meaningful for the professional development of pharmacists in palliative care medicine.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"88 1","pages":"499-504"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88546478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Exploratory data analysis of the dependencies between skin permeability, molecular weight and log P. 皮肤通透性、分子量与log P相关性的探索性数据分析。

Die Pharmazie. Beihefte Pub Date : 2016-06-01 DOI: 10.1691/ph.2015.5170

D. Kilian, H. J. Lemmer, M. Gerber, J. D. du Preez, J. du Plessis

{"title":"Exploratory data analysis of the dependencies between skin permeability, molecular weight and log P.","authors":"D. Kilian, H. J. Lemmer, M. Gerber, J. D. du Preez, J. du Plessis","doi":"10.1691/ph.2015.5170","DOIUrl":"https://doi.org/10.1691/ph.2015.5170","url":null,"abstract":"Molecular weight and log P remain the most frequently used physicochemical properties in models that predict skin permeability. However, several reports over the past two decades have suggested that predictions made by these models may not be sufficiently accurate. In this study, exploratory data analysis of the probabilistic dependencies between molecular weight, log P and log Kp was performed on a dataset constructed from the combination of several popular datasets. The results suggest that, in general, molecular weight and log P are poorly correlated to log Kp. However, after employing several exploratory data analysis techniques, regions within the dataset of statistically significant dependence were identified. As an example of the applicability of the information extracted from the exploratory data analyses, a multiple linear regression model was constructed, bounded by the ranges of dependence. This model gave reasonable approximations to log Kp values obtained from skin permeability studies of selected non-steroidal ant-inflammatory drugs (NSAIDs) administered from a buffer solution and a lipid-based drug delivery system. A method of testing whether a given drug falls within the regions of statistical dependence was also presented. Knowing the ranges within which molecular weight and log P are statistically related to log Kp can supplement existing methods of screening, risk analysis or early drug development decision making to add confidence to predictions made regarding skin permeability.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"28 1","pages":"311-9"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87991150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effects of mPEG proportion and LA/GA ratio on degradation and drug release behaviors of PLGA-mPEG microparticles. mPEG比例和LA/GA比对PLGA-mPEG微颗粒降解和药物释放行为的影响。

Die Pharmazie. Beihefte Pub Date : 2016-05-01 DOI: 10.1691/PH.2016.5179

Chen Shi, Ping Liu, Xianzhe Liu, Xiaobo Feng, Dehao Fu

引用次数: 6

Anti-tumor activity of benzylideneacetophenone derivatives via proteasomal inhibition in prostate cancer cells. 苯乙酮衍生物通过蛋白酶体抑制前列腺癌细胞的抗肿瘤活性。

Die Pharmazie. Beihefte Pub Date : 2016-05-01 DOI: 10.1691/PH.2016.5845

Yun-Hee Lee, Jaesuk Yun, Jae-chul Jung, Seikwan Oh, Young-Suk Jung

引用次数: 5

A novel RP-HPLC method for the detection and quantification of roxithromycin in topical delivery studies. 一种新型的反相高效液相色谱法用于局部给药研究中罗红霉素的检测和定量。

Die Pharmazie. Beihefte Pub Date : 2016-04-01 DOI: 10.1691/PH.2016.5165

M. Aucamp, C. Csongradi, M. Gerber, J. du Plessis

引用次数: 5

Effect of cyclodextrins on the degradation rate of benzylpenicillin. 环糊精对青霉素降解率的影响。

Die Pharmazie. Beihefte Pub Date : 2016-02-01 DOI: 10.26226/morressier.57d6b2bbd462b8028d88d10c

A. Popielec, É. Fenyvesi, K. Yannakopoulou, T. Loftsson

{"title":"Effect of cyclodextrins on the degradation rate of benzylpenicillin.","authors":"A. Popielec, É. Fenyvesi, K. Yannakopoulou, T. Loftsson","doi":"10.26226/morressier.57d6b2bbd462b8028d88d10c","DOIUrl":"https://doi.org/10.26226/morressier.57d6b2bbd462b8028d88d10c","url":null,"abstract":"The effect of cyclodextrin (CD) inclusion complexes on the degradation of benzylpenicillin in aqueous solutions was investigated at several different pH values and 37°C. The effects of neutral as well as both positively and negatively charged CDs were evaluated; all together 13 different CDs. Kinetic studies with HPβCD and RMβCD at pH ranging from 1.2 to 9.6 showed that CDs have stabilizing effect on the β-lactam ring in aqueous acidic media but generally accelerated the hydrolytic cleavage of the β-lactam ring in neutral and basic media. At physiologic pH (pH 7.4) quaternary ammonium CD derivatives (i.e., positively charged CD derivatives) have the highest catalytic effect, resulting in 6- to 18-fold enhancement of hydrolysis rate, while both the neutral methylated CDs had much less effect, resulting in 2- to 3-fold enhancement, and the negatively charged CD derivatives, resulting in only about 1.1- to 1.2-fold enhancement in the hydrolytic cleavage of the β-lactam ring. Addition of water-soluble polymers to the aqueous reaction media containing CDs was shown to decrease the catalyzing effects of CDs on the β-lactam hydrolysis.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"13 1","pages":"68-75"},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79646389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Therapeutic delivery of RNA effectors: diseases affecting the respiratory system. RNA效应物的治疗递送:影响呼吸系统的疾病。

Die Pharmazie. Beihefte Pub Date : 2016-01-01 DOI: 10.1691/PH.2016.5740

Rima Kandil, M. Merkel

引用次数: 8

The influence of passage number for Caco2 cell models when evaluating P-gp mediated drug transport. 评价P-gp介导的药物转运时，传代数对Caco2细胞模型的影响。

Die Pharmazie. Beihefte Pub Date : 2015-12-01 DOI: 10.1691/PH.2015.5106

S. Senarathna, A. Crowe

{"title":"The influence of passage number for Caco2 cell models when evaluating P-gp mediated drug transport.","authors":"S. Senarathna, A. Crowe","doi":"10.1691/PH.2015.5106","DOIUrl":"https://doi.org/10.1691/PH.2015.5106","url":null,"abstract":"Caco2 cells are a human adenocarcinoma cell line that forms tight junctions and are widely used to examine bidirectional drug transport as well as P-glycoprotein mediated efflux. Unfortunately Caco2 cell lines can be very heterogeneous in nature. Our aim was to improve the Caco2 cell model for determination of P-glycoprotein mediated drug transport. Young passage Caco2 from ATCC had inadequate expression of P-glycoprotein, therefore three approaches were adopted to upregulate Caco2 P-glycoprotein expression to mimic that in vivo; a) incubation of mature Caco2 monolayer with rifampicin, b) prolonged exposure of Caco2 cells to vinblastine (generating the Caco2 VIN line), and c) splitting cells every 7 to 9 days until late passage numbers (over P80) were available. Upon development of the models, P-gp expression and activity was determined using western blotting and bidirectional transport studies of rhodamine123. All four models exhibited P-gp mediated efflux transport for rhodamine123. Incubation with rifampicin did not alter bidirectional transport compared to passage 44 cells. Increased passage number altered P-glycoprotein expression and the efflux ratio increased to 4.7 for passage 80 from 1.4 of passage 44. The highest basolateral to apical transport was observed for both passage 89 Caco2 and the Caco2 VIN model with an efflux ratio of 13 to 14. Western blot images confirmed the increased P-glycoprotein expression of late passage and Caco2 VIN. Caco2 cells are not ready for P-gp related research when first acquired from ATCC (Passage 18). Late passage Caco2 cell monolayers or Caco2 VIN models are needed to determine P-gp mediated efflux transport.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"34 1","pages":"798-803"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84535119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16