Die Pharmazie. Beihefte最新文献

Comparison of the Antiemetic Effect of Aprepitant/granisetron and Palonosetron Combined with Dexamethasone in Gynecological Cancer Patients Treated with Paclitaxel and Carboplatin Combination Regimen. 阿瑞吡坦/格拉司琼与帕洛诺司琼联合地塞米松在妇科肿瘤紫杉醇卡铂联合治疗中的止吐效果比较

Die Pharmazie. Beihefte Pub Date : 2022-05-01 DOI: 10.1691/ph.2022.12000

K. Obayashi, A. Nagamine, H. Yashima, S. Ohshima, C. Uchiyama, E. Takahashi, Y. Takahashi, T. Araki, K. Yamamoto

{"title":"Comparison of the Antiemetic Effect of Aprepitant/granisetron and Palonosetron Combined with Dexamethasone in Gynecological Cancer Patients Treated with Paclitaxel and Carboplatin Combination Regimen.","authors":"K. Obayashi, A. Nagamine, H. Yashima, S. Ohshima, C. Uchiyama, E. Takahashi, Y. Takahashi, T. Araki, K. Yamamoto","doi":"10.1691/ph.2022.12000","DOIUrl":"https://doi.org/10.1691/ph.2022.12000","url":null,"abstract":"A triple antiemetic therapy combining aprepitant (APR) with conventional double antiemetic therapy, including 5-hydroxytryptamine 3 receptor antagonist (5-HT₃-RA) and dexamethasone (DEX), is recommended for preventing chemotherapy-induced nausea and vomiting induced by a carboplatin (CBDCA) regimen. However, consensus on the additive effects of APR for gynecological patients on a combined regimen of paclitaxel and CBDCA (TC regimen) has yet to be reached. This retrospective study investigated the antiemetic effects of palonosetron and DEX (PD therapy) and granisetron and DEX with APR (GDA therapy) in patients with gynecologic cancer and who underwent their first TC regimen cycle between April 2017 and March 2020 at the Gunma University Hospital Outpatient Chemotherapy Center. The results showed that the complete response rate of the 92 patients who underwent PD therapy (PD group) and the 46 patients who underwent GDA therapy (GDA group) were both 80.4% (p = 1.000), and the complete control rates of the PD and GDA groups were 78.3% and 80.4%, respectively (p = 0.828), resulting in no significant difference. Furthermore, we observed no significant difference between the PD and GDA groups in the incidence of grade ≥2 nausea, vomiting, and anorexia (nausea: 7.6% vs. 0%, p = 0.095; vomiting: 4.3% vs. 0%, p = 0.301; and anorexia: 9.8% vs. 2.2%, p = 0.164). Concerning adverse events, compared to the PD group, the GDA group showed significantly higher incidence of grade ≥2 malaise (7.6% vs. 19.6%, p = 0.039). Given the lack of difference in the antiemetic effects of PD and GDA therapies, antiemetic therapy should be selected carefully for individual patients by accounting for the incidence of adverse reactions and interactions with APR.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"465 1","pages":"157-161"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77737922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Internalization of FITC-albumin in Human Adipose-derived Stem Cells: Involvement of Clathrin and Caveolin. fitc白蛋白在人脂肪干细胞中的内化:网格蛋白和小窝蛋白的参与。

Die Pharmazie. Beihefte Pub Date : 2022-05-01 DOI: 10.1691/ph.2022.2340

Hongxin Sun, Yumiko Urakami -Takebayashi, H. Motohashi, Junya Nagai

{"title":"Internalization of FITC-albumin in Human Adipose-derived Stem Cells: Involvement of Clathrin and Caveolin.","authors":"Hongxin Sun, Yumiko Urakami -Takebayashi, H. Motohashi, Junya Nagai","doi":"10.1691/ph.2022.2340","DOIUrl":"https://doi.org/10.1691/ph.2022.2340","url":null,"abstract":"Adipose tissue-derived stem cells (AdSCs) are one of the most promising cell types for cell-based therapies. In addition, AdSCs systematically injected into the body have been reported to localize to damaged tissues and certain types of tumor. As an important part of establishing a potent drug delivery system with AdSCs, the mechanism and efficiency of uptake into AdSCs has drawn much research attention. However, this remains to be fully clarified. The aim of this study was to examine the characteristics of endocytosis-mediated uptake in human AdSCs. We used fluorescein isothiocyanate-labeled albumin (FITC-albumin) as a potent marker of endocytosis. FITC-albumin uptake was time- and temperature-dependent. Confocal microscopy showed punctate localization of fluorescence in the cytoplasm. FITC-albumin uptake was inhibited by human serum albumin in a concentration-dependent manner. FITC-albumin uptake was inhibited by a metabolic inhibitor (2,4-dinitrophenol), a microtubule polymerization inhibitor (colchicine), an actin polymerization inhibitor (cytochalasin D), endosomal acidification inhibitors (chloroquine and bafilomycin A1), clathrin-dependent endocytosis inhibitors (chloropromazine, phenylarsine oxide, and Pitstop2), and caveolin-dependent endocytosis inhibitors (nystatin and methyl-β-cyclodextrin). Furthermore, the knockdown of the clathrin heavy chain and caveolin-1 significantly reduced FITC-albumin uptake. These findings suggest that AdSCs take up albumin via endocytic pathways in which clathrin and caveolin are involved.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"38 1","pages":"141-146"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81293516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Pharmacist-led Deprescribing Intervention with the Functional Recovery in Convalescent Setting. 以药剂师为主导的减处方干预与恢复期功能恢复的关联。

Die Pharmazie. Beihefte Pub Date : 2022-05-01 DOI: 10.1691/ph.2022.2323

E. Kose, H. Endo, H. Hori, S. Hosono, C. Kawamura, Y. Kodama, T. Yamazaki, N. Yasuno

{"title":"Association of Pharmacist-led Deprescribing Intervention with the Functional Recovery in Convalescent Setting.","authors":"E. Kose, H. Endo, H. Hori, S. Hosono, C. Kawamura, Y. Kodama, T. Yamazaki, N. Yasuno","doi":"10.1691/ph.2022.2323","DOIUrl":"https://doi.org/10.1691/ph.2022.2323","url":null,"abstract":"So far, no studies investigated the association between pharmacist intervention and rehabilitation outcomes. The aim of study was to establish whether the pharmacist-led deprescribing intervention affects rehabilitation outcomes. This retrospective, observational, single-center, cohort study included consecutive geriatric patients (n = 448) with pharmacist-led intervention between 2017 and 2019. Participants were divided based on pharmacist-led deprescribing and non deprescribing interventions during hospitalization. Demographic data, laboratory data, the Functional Independence Measure were (FIM) analyzed between the groups. Multiple linear regression analysis was performed to analyze the relationship between pharmacist-led deprescribing and FIM total gain. The primary outcome was FIM total gain. The rate of pharmacist intervention during the study period was 92.4%. A multiple linear regression analysis of FMI-T gain, adjusting for confounding factors, revealed that the pharmacist-led deprescribing intervention was independently correlated with FMI-T gain. Particularly, the use of dyslipidemia drugs, antipsychotic drugs, hypnotics, and nonsteroidal anti-inflammatory drugs significantly decreased during hospitalization. The pharmacist-led deprescribing intervention was independently and significantly associated with FIM-T gain. The pharmacist-led deprescribing intervention improved functional recovery in a rehabilitation setting.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"29 1","pages":"165-170"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75681875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulatory Effects of Sinensetin on Macrophage and Cyclophosphamide-induced Immunosuppression in Mice. 青肠素对小鼠巨噬细胞和环磷酰胺诱导的免疫抑制的免疫调节作用。

Die Pharmazie. Beihefte Pub Date : 2022-05-01 DOI: 10.1691/ph.2022.2321

Yi-Lun Wang, Jin-Jie Yang, W. Ni

引用次数: 4

Selective Growth Suppressive Effect of Pravastatin on Senescent Human Lung Fibroblasts. 普伐他汀对衰老人肺成纤维细胞的选择性生长抑制作用。

Die Pharmazie. Beihefte Pub Date : 2022-05-01 DOI: 10.1691/ph.2022.2327

H. Ushijima, A. Onodera

{"title":"Selective Growth Suppressive Effect of Pravastatin on Senescent Human Lung Fibroblasts.","authors":"H. Ushijima, A. Onodera","doi":"10.1691/ph.2022.2327","DOIUrl":"https://doi.org/10.1691/ph.2022.2327","url":null,"abstract":"Various chemical reagents containing inhibitors of mitochondrial activity, antioxidants, nuclear factor-kappa B (NF-kB) inhibitor, mammalian target of rapamycin (mTOR) inhibitor and other clinical therapeutics were screened in order to identify those that selectively decrease the viability of senescent human lung fibroblasts. Cell viability was measured using the CCK-8 assay. The results showed that pravastatin, a drug for hyperlipidemia, decreased the viability of senescent cells but not non-senescent cells. The effect of pravastatin on senescent cells is thought to be due to the inhibition of cell proliferation, rather than cell death. The effect of pravastatin was further investigated using the glucose metabolism assay, which showed that glucose consumption was inhibited both in non-senescent and senescent cells and intracellular nicotinamide adenine dinucleotide (NAD) was decreased in senescent cells. Changes to the mRNA expression levels of senescence-associated genes in response to pravastatin treatment were quantified by real-time-qPCR. There were no significant changes in the relative mRNA expression levels of IL-1β, p16, p21, and p53 in pravastatin-treated non-senescent cells, whereas the expression of IL-1β and p16 were increased by pravastatin only in senescent cells. The results of this study suggest that pravastatin does not induce senolysis, but rather selectively inhibits the proliferation of senescent cells and that cellular senescence is enhanced by decreasing intracellular NAD and promoting IL-1β production.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"875 1","pages":"132-136"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72663793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Expression of Vincristin-induced Peripheral Neuropathy Related to Different Administration Methods. 长春新素诱导的周围神经病变在不同给药方式下的表达。

Die Pharmazie. Beihefte Pub Date : 2022-05-01 DOI: 10.1691/ph.2022.2329

T. Fukuoka, Y. Yamamoto, E. Usami, H. Hayashi, J. Utsunomiya, M. Kimura, M. Nakamura, T. Yoshimura, Y. Toda

{"title":"Expression of Vincristin-induced Peripheral Neuropathy Related to Different Administration Methods.","authors":"T. Fukuoka, Y. Yamamoto, E. Usami, H. Hayashi, J. Utsunomiya, M. Kimura, M. Nakamura, T. Yoshimura, Y. Toda","doi":"10.1691/ph.2022.2329","DOIUrl":"https://doi.org/10.1691/ph.2022.2329","url":null,"abstract":"Vincristine (VCR) is an important drug used in R-CHOP regimens for the treatment of non-Hodgkin's lymphoma. The purpose of this study was to examine whether the administration method affects the incidence of VCR-induced peripheral neuropathy. We investigated the ratio of VCR-induced peripheral neuropathy during rapid intravenous infusion and intravenous drip infusion. A total of 71 patients who had received six or more courses of R-CHOP from January, 2015 to December, 2016 at Komaki City Hospital and Ogaki Municipal Hospital were retrospectively investigated. Peripheral neuropathy was observed in 27/39 patients (69 %) and 24/32 (75 %) in rapid intravenous infusion and intravenous drip infusion of VCR, respectively (P = 0.79). Peripheral neuropathy was observed at a high frequency in this study. Additionally, there was no difference in frequency of peripheral neuropathy due to the difference in administration method. In both groups, the degree of peripheral neuropathy was grade 1 and grade 2 in most patients. However, in rapid intravenous infusion, grade 3 peripheral neuropathy was observed. Some cases required dose reduction and discontinuation in rapid intravenous infusion. In contrast, there were no discontinuing patients in the intravenous drip infusion. Therefore, it was suggested that intravenous drip infusion of VCR reduced serious peripheral neuropathy because the ratio requiring dose reduction and discontinuation was less than that in the rapid group. In conclusion, this study is informative as there are few reports focusing on the administration method of vincristine.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"24 1","pages":"162-164"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81076507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activin Suppresses the Inflammatory Response of TNF-α -stimulated Human Umbilical Vein Endothelial Cells. 激活素抑制TNF-α刺激的人脐静脉内皮细胞的炎症反应。

Die Pharmazie. Beihefte Pub Date : 2022-05-01 DOI: 10.1691/ph.2022.2330

H. Ko, Young Il Kim, H. Ahn

{"title":"Activin Suppresses the Inflammatory Response of TNF-α -stimulated Human Umbilical Vein Endothelial Cells.","authors":"H. Ko, Young Il Kim, H. Ahn","doi":"10.1691/ph.2022.2330","DOIUrl":"https://doi.org/10.1691/ph.2022.2330","url":null,"abstract":"Activins belong to the transforming growth factor (TGF)-β superfamily and are involved in the regulation of homeostasis, proliferation, differentiation, and inflammation. In the present study, we examined the mechanism by which activin regulates the transcription of tumor necrosis factor-α (TNF-α)-stimulated cytokines, chemokines, toll-like receptors (TLRs), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in human umbilical vein endothelial cells (HUVECs), and the involvement of the nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Cell viability was analyzed using MTS/PES solution, mRNA expression was measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and protein expression was measured by immunoblotting. TNF-α increased the mRNA expression of cytokines (IL-1β and IL-6), chemokines (IL-8 and MCP-1), and TLR2, as well as the mRNA and protein expression of iNOS and COX-2. Activin decreased TNF-α-induced cytokine, chemokine, and TLR mRNA expression as well as TNF-α-induced iNOS and COX-2 mRNA and protein expression. In addition, activin suppressed the phosphorylation of NF-κB p65 in TNF-α-stimulated HUVECs and reduced TNF-α-induced phosphorylation of AKT, JNK, ERK, and p38 MAPK. Our results demonstrate that the anti-inflammatory effects of activin are mediated by inflammatory response genes through the inhibition of NF-κB and AKT/JNK/MAPK signaling.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"17 1","pages":"152-156"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84804630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ITGB1 Suppresses Autophagy Through Inhibiting The mTORC2/AKT Signaling Pathway In H9C2 Cells. ITGB1通过抑制H9C2细胞mTORC2/AKT信号通路抑制自噬。

Die Pharmazie. Beihefte Pub Date : 2022-05-01 DOI: 10.1691/ph.2022.2351

Weiwei Zhou, Weizhe Liu, Dingyan Zhou, Aiying Li

引用次数: 2

A novel and simple dynamic coating capillary electrophoresis method for the chiral separation and quantification of mitiglinide enantiomers using hydroxyethyl cellulose as a dynamic coating agent. 以羟乙基纤维素为动态包衣剂，建立了一种新的、简单的动态包衣毛细管电泳方法，用于米格列奈对映体的手性分离和定量。

Die Pharmazie. Beihefte Pub Date : 2022-04-10 DOI: 10.1691/ph.2022.1166

K. M. Azzam

{"title":"A novel and simple dynamic coating capillary electrophoresis method for the chiral separation and quantification of mitiglinide enantiomers using hydroxyethyl cellulose as a dynamic coating agent.","authors":"K. M. Azzam","doi":"10.1691/ph.2022.1166","DOIUrl":"https://doi.org/10.1691/ph.2022.1166","url":null,"abstract":"A capillary electrophoretic method for the chiral separation and quantification of mitiglinide (MTG) enantiomers is described (less than 9.5 min) with resolution value Rs = 5.25 and with excellent peak shapes after performing the dynamically coating for the bare fused capillary. The study aims to develop and validate a novel and simple method for the separation and quantification of MTG enantiomers using CE after dynamic coating the capillary wall using the hydroxyethyl cellulose (HEC) coating agent. Dynamic coating procedure of the capillary inner surface is conducted via rapid flushes using 0.1 M sodium hydroxide, water, and aqueous solution containing HEC, and hydroxypropyl-γ-cyclodextrin (HP-γ-CD). Besides buffer was used for the dynamic coating process in addition to its use as the separation medium. When the dynamic coating was used, peak symmetry was improved. A bare fused-silica capillary was used throughout the separation after being coated using HEC dissolved in the background electrolyte (BGE) of 50 mM Na₂HPO₄ - 1 M H₃PO₄ solution; pH 8.5; containing 25 25 mg mL-1 of each HP-γ-CD and HEC. The dynamic coating procedure achieved an improvement in migration time as well as peak area precision. The adsorbed coating agent showed slight interactions with MTG, providing efficient separation with outstanding durability and reproducibility at slightly alkaline conditions (pH 8.5). Acceptable validation criteria for selectivity, linearity, precision, and accuracy were also studied. The newly developed method was effectively applied to the assay of enantiomers of MTG in pharmaceutical formulations. Additionally, it was proven to have the advantages of being simple, rapid, and accurate.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"2 1","pages":"95-102"},"PeriodicalIF":0.0,"publicationDate":"2022-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87972330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of batch size on the granule properties in planetary centrifugal granulation. 物料粒度对行星离心造粒中颗粒性能的影响。

Die Pharmazie. Beihefte Pub Date : 2022-04-10 DOI: 10.1691/ph.2022.11059

Y. Miyazaki, T. Uchino, Y. Kagawa

{"title":"Effect of batch size on the granule properties in planetary centrifugal granulation.","authors":"Y. Miyazaki, T. Uchino, Y. Kagawa","doi":"10.1691/ph.2022.11059","DOIUrl":"https://doi.org/10.1691/ph.2022.11059","url":null,"abstract":"A granulation method using a planetary centrifugal mixer, called planetary centrifugal granulation, has been developed for small-scale production, such as extemporaneous preparation in pharmacies. Although the impact of its operational parameters on granulation is described, the scale effect has not been investigated. Therefore, we aimed to reveal the effects of vessel size and vessel filling rate on granule properties. In this study, ibuprofen 20% granules consisting of lactose, cornstarch, sodium carmellose, and talc were used as model granules. Granulation was performed using geometrically similar containers, 6-58 mL, with a filling rate of 20-70%. After granulation, we monitored the granule properties, for example, median diameter (d50), span of particle size distribution, and sphericity. At filling rates of 40% and 50% in the 58-mL vessel, the granules grew larger in diameter, and at a rate of 30%, the granules showed a higher sphericity. When the filling rate was 30%, d50 became larger and the span decreased as the vessel size increased. The yields of the granules were higher than 95% when using the 12-58 mL vessel. Lastly, the drug content uniformity and drug dissolution behavior of the granules produced in different vessel size were examined. The granules showed similar drug consistencies and drug dissolution profiles. In conclusion, the quality of the products was not affected by changes in vessel size. Thus, pharmacists could prepare and compound the granule formulations with high yield and appropriate quality using an adequate vessel in the same manner.","PeriodicalId":86039,"journal":{"name":"Die Pharmazie. Beihefte","volume":"4 1","pages":"103-106"},"PeriodicalIF":0.0,"publicationDate":"2022-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91148985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0