Australian journal of biological sciences最新文献_第8页

Temperature and humidity of expired air of sheep. 绵羊呼出空气的温度和湿度。

Australian journal of biological sciences Pub Date : 1988-01-01 DOI: 10.1071/bi9880309

K G Johnson, S M Callahan, R Strack

引用次数: 7

Development of sheep embryos in vitro in a medium supplemented with different batches of serum albumin. 绵羊胚胎在添加不同批次血清白蛋白培养基中的体外发育。

Australian journal of biological sciences Pub Date : 1988-01-01 DOI: 10.1071/bi9880371

P A Batt, B G Miller

引用次数: 7

Control of steroidogenesis in small and large bovine luteal cells. 牛黄体小细胞和大细胞类固醇生成的控制。

Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/bi9870331

W Hansel, H W Alila, J P Dowd, X Z Yang

{"title":"Control of steroidogenesis in small and large bovine luteal cells.","authors":"W Hansel, H W Alila, J P Dowd, X Z Yang","doi":"10.1071/bi9870331","DOIUrl":"https://doi.org/10.1071/bi9870331","url":null,"abstract":"Evidence was cited to show that: (1) prostacyclin (PGI2) plays a luteotrophic role in the bovine corpus luteum and that products of the lipoxygenase pathway of arachidonic acid metabolism, especially 5-hydroxyeicosatetraenoic acid play luteolytic roles; (2) oxytocin of luteal cell origin plays a role in development, and possibly in regression, of the bovine corpus luteum; and (3) luteal cells arise from two sources; the characteristic small luteal cells at all stages of the oestrous cycle and pregnancy are of theca cell origin; the large cells are of granulosa cell origin early in the cycle, but a population of theca-derived large cells appears later in the cycle. Results of in vitro studies with total dispersed cells and essentially pure preparations of large and small luteal cells indicate that: (1) the recently described Ca2+-polyphosphoinositol-protein kinase C second messenger system is involved in progesterone synthesis in the bovine corpus luteum; (2) activation of protein kinase C is stimulatory to progesterone synthesis in the small luteal cells; (3) activation of protein kinase C has no effect on progesterone synthesis in the large luteal cells; and (4) protein kinase C exerts its luteotrophic effect in total cell preparations, in part at least, by stimulating the production of prostacyclin. The protein kinase C system may cause down regulation of LH receptors in the large cells.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1071/bi9870331","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14455138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 85

Tammar wallaby plasma protease inhibitory (Pi) proteins. 袋鼠血浆蛋白酶抑制蛋白。

Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/bi9870355

S D Patterson, K Bell, W E Poole

引用次数: 15

Effects of maternal nutritional status on fetal and placental growth and on fetal urea synthesis in sheep. 母体营养状况对绵羊胎儿和胎盘生长及胎儿尿素合成的影响。

Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/bi9870365

G J Faichney, G A White

引用次数: 108

Structural differentiation and fluid reabsorption in the ductuli efferentes testis of the rat. 大鼠睾丸输尿管的结构分化和液体重吸收。

Australian journal of biological sciences Pub Date : 1987-01-01

R C Jones, K M Jurd

{"title":"Structural differentiation and fluid reabsorption in the ductuli efferentes testis of the rat.","authors":"R C Jones, K M Jurd","doi":"","DOIUrl":"","url":null,"abstract":"The ductuli efferentes testis of the rat form a cord which is embedded in adipose tissue. The cord is anatomically differentiated into a proximal cylindrical region, the initial zone, and an ampulla, the coni vasculosi. The initial zone contains six or seven ductuli which leave the rete testis and run in a sinuous path, roughly parallel with one another. However, the ductuli in the coni vasculosi are more sinuous than in the initial zone and they anastomose; pairs join together to form ultimately a single, common ductulus efferens. Stereological studies of paraffin sections and electron micrographs showed that the differentiation of the ductuli into two parts can be recognized at tissue and cellular levels of organization. Stereological and micropuncture studies showed that the ductuli efferentes reabsorb most of the fluid leaving the testis and it was concluded that most reabsorption occurred in the initial zone. It was estimated that the rate of fluid absorption is greater in the ductuli efferentes than in the proximal convoluted tubules of the kidney. The mechanism of fluid transport across the mucosa of the ductuli is considered in the Discussion. It is concluded that transport in vesicles and vacuoles could not account for the rate of fluid reabsorption and that the main mechanism of transport probably involves the coupling of water and active salt transport.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14632002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-esterified long-chain fatty acid metabolism in fed sheep at rest and during exercise. 非酯化长链脂肪酸在饲羊休息和运动中的代谢。

Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/bi9870221

D W Pethick, N Harman, J K Chong

{"title":"Non-esterified long-chain fatty acid metabolism in fed sheep at rest and during exercise.","authors":"D W Pethick, N Harman, J K Chong","doi":"10.1071/bi9870221","DOIUrl":"https://doi.org/10.1071/bi9870221","url":null,"abstract":"The role of circulating, non-esterified, long-chain fatty acids (NEFA) as a source of energy for the whole animal and skeletal muscle was investigated in fed non-pregnant sheep at rest and during exercise. Infusion of tracer quantities of [1-14C]oleic or [1-14C]stearic acid was combined with the use of arteriovenous difference studies on fed sheep at rest or during a 2 h period of exercise on a belt treadmill moving at 4.5 km h-1. At rest all parameters of NEFA metabolism indicated a minimal role for oxidation. Thus the concentration in plasma (0.07 +/- 0.01 mmol l-1), entry rate (0.08 +/- 0.02 mmol h-1 kg-1 body wt), contribution to whole animal oxidation (1.2 +/- 0.3%) and utilization of NEFA by skeletal muscle (0.046 +/- 0.008 mmol h-1 kg-1 muscle) were all low. Exercise prompted a shift to lipolysis and accordingly the above parameters increased markedly some 13-24-fold. The circulating concentration of ketone bodies showed only a small increase during exercise and consequently the role of ketone bodies as an energy source during exercise was minimal. Glucose utilization by skeletal muscle was considerable in animals at rest and it represented the most significant potential fuel of skeletal muscle. Exercise resulted in a sustained increase of 3-4-fold in the utilization of glucose by skeletal muscle. Thus the traditional view that NEFA and not glucose is a predominant fuel of skeletal muscle of fed sheep should be appraised.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1071/bi9870221","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14783552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Stimulation of the hypothalamic-pituitary-adrenal axis and inhibition of growth hormone release via increased central noradrenaline neuronal activity by urethane anaesthesia in the rat: blockade by clonidine. 氨基甲酸乙酯麻醉对大鼠下丘脑-垂体-肾上腺轴的刺激和通过增加中枢去甲肾上腺素神经元活性抑制生长激素的释放:可定阻断。

Australian journal of biological sciences Pub Date : 1987-01-01

G A Smythe, R M Gleeson, B H Stead

{"title":"Stimulation of the hypothalamic-pituitary-adrenal axis and inhibition of growth hormone release via increased central noradrenaline neuronal activity by urethane anaesthesia in the rat: blockade by clonidine.","authors":"G A Smythe, R M Gleeson, B H Stead","doi":"","DOIUrl":"","url":null,"abstract":"Computerized gas chromatography-mass spectrometry was used to measure precisely the hypothalamic levels of noradrenaline (NA), dopamine and serotonin together with those of their major neuronal metabolites 3,4-dihydroxyphenylethyleneglycol (DHPG), 3,4-dihydroxyphenylacetic acid and 5-hydroxyindoleacetic acid in normal male rats 45 min after stimulation of hypothalamic-pituitary-adrenal function by urethane (1.3 g/kg) administration. Urethane treatment resulted in a significant elevation of central noradrenergic neuronal activity (NNA) as assessed from marked rises in hypothalamic DHPG concentrations and the ratio (DHPG/NA). At the same time there was significant stimulation of ACTH and corticosterone release and inhibition of growth hormone release. These hormonal and central effects of urethane (but not anesthesia) were inhibited when the alpha 2-agonist clonidine (150 micrograms/kg) was co-administered. Urethane had no major effect on hypothalamic dopamine or serotonin status. We propose that the release of ACTH and the suppression of growth hormone release following urethane anaesthesia is a result of activation of central NNA and suggest that the hormonal responses are mediated via hypothalamic noradrenergic facilitation of corticotrophin releasing factor and somatostatin release to the anterior pituitary.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14402750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rapid, sensitive and reliable assay for inhibin bioactivity. 一种快速、灵敏、可靠的抑制素生物活性测定方法。

Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/BI9870105

V. Lee, N. Colvin, H. Quigg, L. Atley, J. McMaster, L. Leversha, H. Burger

{"title":"A rapid, sensitive and reliable assay for inhibin bioactivity.","authors":"V. Lee, N. Colvin, H. Quigg, L. Atley, J. McMaster, L. Leversha, H. Burger","doi":"10.1071/BI9870105","DOIUrl":"https://doi.org/10.1071/BI9870105","url":null,"abstract":"A rapid 2-day quantitative assay for inhibin bioactivity based on FSH secretion from pituitary cells of immature female rats is described. The bioassay exhibited steeper slopes, improved precision and greater (fourfold) sensitivity compared with a previously established pituitary FSH cell content assay. Whole pituitary glands were used for the preparation of pituitary cells and the method for cell dispersion required a single enzymatic treatment with trypsin. Cells (180,000 viable cells per well) were dispensed into culture media containing inhibin and incubated for 48 h. Media were removed and assayed for FSH by radioimmunoassay. Using a ram rete testis fluid preparation as standard the inhibin dose-response curves of 25 consecutive experiments showed indices of precision of -0.08(mean)[range -0.04 to -0.17] and Finney's G values of 0.017[0.003-0.06]. The mean ED40 was 0.17 units of inhibin activity per well with interassay variation of 16.2% at this point of the dose-response curve. The assay had a practical capacity of 400 wells, permitting the measurement of dose-response curves of at least 40 unknowns with three dose points and triplicate wells per dose. The assay is specific for inhibin-containing preparations from several animal species. Overall, the assay is simple, precise, and sensitive, indicative of its applicability to the measurement of inhibin samples with low inhibin bioactivity and to the screening of large numbers of fractions during inhibin purification.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78981397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Isolation of two distinct activator proteins for lipoprotein lipase from ovine plasma. 绵羊血浆中两种不同的脂蛋白脂肪酶激活蛋白的分离。

Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/bi9870235

R K Tume, R F Thornton, G W Johnson

{"title":"Isolation of two distinct activator proteins for lipoprotein lipase from ovine plasma.","authors":"R K Tume, R F Thornton, G W Johnson","doi":"10.1071/bi9870235","DOIUrl":"https://doi.org/10.1071/bi9870235","url":null,"abstract":"Two distinct activator proteins for lipoprotein lipase (LPL) have been isolated in approximately equal amounts from ovine plasma. These low molecular weight proteins were readily separated from each other on the basis of size and charge. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated proteins of Mr about 8000 and 5000, with pI in urea-containing gels of about 5.1 and 4.8 respectively. Each of the ovine activator proteins was as effective as human apolipoprotein C-II (apo C-II) in activating LPL, with 1 microgram/ml giving near to maximum activation, and in lowering the apparent Km of LPL for triolein substrate. As the ratio of activator to triolein increased from 0.16 to 5.2 (micrograms/mg) the apparent Km fell from about 0.5 to 0.18 mM. Whereas human apo C-II and the two ovine activators were equally effective in stimulating the hydrolysis of triolein, differences were observed between the human and ovine activators when p-nitrophenylbutyrate was used as substrate. Unlike human apo C-II, which produced significant inhibition of p-nitrophenylbutyrate hydrolysis, the ovine activators were without effect. This suggests that the interaction between the ovine activators and LPL is different from that of human apo C-II.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14566881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0