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Effect of erythrocyte spectrin on actin self-association. 红细胞谱素对肌动蛋白自我结合的影响。
Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/BI9870027
L. Tilley, G. Ralston
{"title":"Effect of erythrocyte spectrin on actin self-association.","authors":"L. Tilley, G. Ralston","doi":"10.1071/BI9870027","DOIUrl":"https://doi.org/10.1071/BI9870027","url":null,"abstract":"The polymerization of pyrene-labelled skeletal muscle actin has been monitored in the presence of chromatographically purified spectrin dimers and tetramers. A small but consistent effect of spectrin binding on the critical concentration was observed for actin polymerized in the presence of 1 mM MgCl2. These data were analysed using the principle of linked functions. Spectrin binds exclusively to the filamentous form of actin, and thereby stabilizes F-actin with respect to the G-form. The decrease in the critical concentration for actin polymerization, in the presence of spectrin, has been shown to be consistent with an equilibrium constant for the binding of spectrin to individual promoters within F-actin of approximately 8 X 10(5) M-1 at 23 degrees C, and an ionic strength of 7 mM.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77880463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Structural differentiation and fluid reabsorption in the ductuli efferentes testis of the rat. 大鼠睾丸输尿管的结构分化和液体重吸收。
Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/BI9870079
R. Jones, K. Jurd
{"title":"Structural differentiation and fluid reabsorption in the ductuli efferentes testis of the rat.","authors":"R. Jones, K. Jurd","doi":"10.1071/BI9870079","DOIUrl":"https://doi.org/10.1071/BI9870079","url":null,"abstract":"The ductuli efferentes testis of the rat form a cord which is embedded in adipose tissue. The cord is anatomically differentiated into a proximal cylindrical region, the initial zone, and an ampulla, the coni vasculosi. The initial zone contains six or seven ductuli which leave the rete testis and run in a sinuous path, roughly parallel with one another. However, the ductuli in the coni vasculosi are more sinuous than in the initial zone and they anastomose; pairs join together to form ultimately a single, common ductulus efferens. Stereological studies of paraffin sections and electron micrographs showed that the differentiation of the ductuli into two parts can be recognized at tissue and cellular levels of organization. Stereological and micropuncture studies showed that the ductuli efferentes reabsorb most of the fluid leaving the testis and it was concluded that most reabsorption occurred in the initial zone. It was estimated that the rate of fluid absorption is greater in the ductuli efferentes than in the proximal convoluted tubules of the kidney. The mechanism of fluid transport across the mucosa of the ductuli is considered in the Discussion. It is concluded that transport in vesicles and vacuoles could not account for the rate of fluid reabsorption and that the main mechanism of transport probably involves the coupling of water and active salt transport.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73823051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 65
Composition of Wax Made by the Australian Stingless Bee Trigona australis 澳洲无刺蜂(Trigona australis)蜡的组成
Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/BI9870015
B. Milborrow, J. Kennedy, A. Dollin
{"title":"Composition of Wax Made by the Australian Stingless Bee Trigona australis","authors":"B. Milborrow, J. Kennedy, A. Dollin","doi":"10.1071/BI9870015","DOIUrl":"https://doi.org/10.1071/BI9870015","url":null,"abstract":"Analysis of the nest material of T. australis by gas chromatography/chemical ionization-mass spectrometry showed the wax to comprise a hydrocarbon fraction (90%), esters (6%) and free acids . (4%). The major saturated hydrocarbons were C27, C31 and C33 with C2S and C29 being less abundant and C23 and C 3S being present in small amounts. Traces of the intermediate, even-numbered homologues were also found. Hydrocarbons (C31o C 33 and C 3S) which contained one double bond were also present and traces of the diene C3S were detected. The ester fraction did not contain compounds identical with those in beeswax made by the honeybee Apis melli/era and the acid fractions were also quite different. T. australis wax contained the following, saturated free acids: CIO (trace), C12, C14, C16, CIS and C20, monoenoic and dienoic CIS and C 20 and traces of the trienoic CIS. The wax of T. australis is colourless but the brown colour of the nest material derives from the inclusion of masses of pollen (Eucalyptus sp.) and solid material from the inside of the nest tree. The solid residue comprised between 12 and 30% by weight.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80067279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Electrophoretic Resolution of Species Boundaries in Australian Microchiroptera. I. Eptesicus (Chiroptera: Vespertilionidae) 澳洲小翼目种界的电泳分辨。1 .夜蛾(翼翅目:夜蛾科)
Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/BI9870143
M. Adams, P. Baverstock, C. Watts, T. Reardon
{"title":"Electrophoretic Resolution of Species Boundaries in Australian Microchiroptera. I. Eptesicus (Chiroptera: Vespertilionidae)","authors":"M. Adams, P. Baverstock, C. Watts, T. Reardon","doi":"10.1071/BI9870143","DOIUrl":"https://doi.org/10.1071/BI9870143","url":null,"abstract":"The technique of allozyme electrophoresis is used in the present study to define species boundaries in Australian Eptesicus. Until 1976, only one species of Eptesicus (E. pumilus) was recognized in Australia. More recently, a further four species have been recognised (E. vulturnus, E. regulus, E. sagittula and E. douglasl). Results obtained from an allozyme electrophoretic analysis of 35 loci in 182 individuals show that there are a minimum of nine species of Eptesicus in Australia. Moreover, in some geographic areas, up to four species occur sympatrically. These results highlight the applicability and significance of allozyme electrophoresis for the dissection of species complexes.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85167789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 58
Immunolocalization Studies of the High-sulfur Protein Fraction of Merino Wool using Monoclonal Antibodies 单克隆抗体对美利奴羊毛高硫蛋白片段的免疫定位研究
Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/BI9870249
P. French, D. Hewish
{"title":"Immunolocalization Studies of the High-sulfur Protein Fraction of Merino Wool using Monoclonal Antibodies","authors":"P. French, D. Hewish","doi":"10.1071/BI9870249","DOIUrl":"https://doi.org/10.1071/BI9870249","url":null,"abstract":"Monoclonal antibodies raised to the high-sulfur fraction of solubilized wool protein were used to study the location of these proteins in sections of Merino sheep skin, using indirect immunofluorescence. Poor antibody binding was observed to untreated wool follicles, but mild trypsin digestion of the sections allowed penetration of the antibodies and specific antibody localization was observed. The high-sulfur protein monoclonal antibodies bound to the cells of the wool cortex in the keratogenous zone, and to the cells of the inner root sheath and fibre cuticle regions. The specificities of the antibodies were determined by their binding to high-sulfur proteins separated using high-performance liquid chromatography.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83846280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Stress-induced changes in plasma concentrations of immunoreactive beta-endorphin and cortisol in response to routine surgical procedures in lambs. 应激引起的羔羊常规外科手术后免疫反应性β -内啡肽和皮质醇血浆浓度的变化
Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/BI9870097
D. Shutt, L. Fell, R. Connell, A. K. Bell, C. Wallace, A. Smith
{"title":"Stress-induced changes in plasma concentrations of immunoreactive beta-endorphin and cortisol in response to routine surgical procedures in lambs.","authors":"D. Shutt, L. Fell, R. Connell, A. K. Bell, C. Wallace, A. Smith","doi":"10.1071/BI9870097","DOIUrl":"https://doi.org/10.1071/BI9870097","url":null,"abstract":"Following four different surgical procedures in lambs 3-5 weeks old, plasma immunoreactive beta-endorphin (beta-EP) and cortisol were assayed at 15 min and 24 h as determinants of post-operative stress. A threefold increase in mean plasma beta-EP levels occurred 15 min after tail docking, and a maximal eight- to tenfold increase occurred in response to castration and/or mulesing with tail docking. Significant increments in mean plasma cortisol levels followed these surgical procedures with the maximal response 15 min after mulesing plus castration with tail docking. The physiologically active 'free' cortisol in plasma represents about 25% of the cortisol, as measured, and the two are highly correlated. At 24 h, beta-EP levels in all treated groups were similar to controls, although a small elevation in cortisol levels was still present in the lambs subjected to mulesing. Ultrafiltration of plasma extracts showed that peak beta-EP levels contained about 40% immunoreactivity from low molecular weight species (mol. wt less than 10,000). By specific radioimmunoassay and reverse-phase high-performance liquid chromatography this comprised about 75% beta-EP1-31, the most potent analgesic endorphin, 10% beta-EP1-27, and 15% alpha-N-acetyl-beta-EP. Increased beta-EP1-31 levels may modulate post-operative pain in lambs.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91266256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 81
Preliminary studies of the complement fixation test to confirm the diagnosis of bovine ephemeral fever. 补体固定试验诊断牛短暂热的初步研究。
Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/BI9870137
W. Bai, F. Tian, C. Wang, C. Jiang, Z. G. Zhang
{"title":"Preliminary studies of the complement fixation test to confirm the diagnosis of bovine ephemeral fever.","authors":"W. Bai, F. Tian, C. Wang, C. Jiang, Z. G. Zhang","doi":"10.1071/BI9870137","DOIUrl":"https://doi.org/10.1071/BI9870137","url":null,"abstract":"A strain of bovine ephemeral fever (BEF) virus isolated in China in 1976 was adapted to growth in tissue cultures. A baby hamster kidney complement fixing (CF) antigen, stable at -20 degrees C for at least 120 days, was prepared from the BEF virus grown in tissue culture and used to test bovine sera for antibodies to that virus. CF antibodies were detected in all of 31 cattle after convalescence from experimental infection with BEF virus, in 208 (98%) of 213 cattle observed to have shown clinical ephemeral fever in an epidemic, in 96 cattle in these herds which did not show clinical signs of ephemeral fever and 16 cattle from herds in northern China outside the epidemic area. The CF antibodies to BEF virus were found to persist in 34 (89%) of 38 cattle which were bled 6 years after natural exposure to ephemeral fever. The CF antigen is economical to prepare and is suitable to differentiate ephemeral fever from other viral infections with which it could possibly be confused on clinical appearance.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83836067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Genetic variation in mitochondrial DNA from some aboriginal Australians. 一些澳大利亚原住民线粒体DNA的遗传变异。
Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/bi9870171
G Griziotis, C Hawkins, A G Mackinlay, R H Crozier
{"title":"Genetic variation in mitochondrial DNA from some aboriginal Australians.","authors":"G Griziotis,&nbsp;C Hawkins,&nbsp;A G Mackinlay,&nbsp;R H Crozier","doi":"10.1071/bi9870171","DOIUrl":"https://doi.org/10.1071/bi9870171","url":null,"abstract":"<p><p>Mitochondrial (mt) DNAs from 17 aboriginal Australians, predominantly from the coastal region of the Northern Territory were isolated and digested with four four-base restriction endonucleases, two of which revealed variation between samples. The observed fragment patterns were used directly in parsimony analyses of phylogenetic relationships between the samples, and were also converted to estimates of the number of substitutions per nucleotide position between samples (delta), which estimates were then used in distance analyses of phylogeny. The inferred fragment patterns of the completely sequenced 'Cambridge' human mtDNA were also included in these analyses. No strong evidence of geographic variation was found, consistent with previous findings of Australian aborigines and other humans generally, although the most divergent sample was one of two from Sydney, indicating that further work is desirable. The estimate of mean difference between samples (diversity), 0.0017 +/- 0.0003 (mean +/- 95% confidence interval), is significantly lower than that reported previously for humans generally.</p>","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14782940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Adrenaline stimulation of phospholipid metabolism in adipocyte ghosts. 肾上腺素刺激脂肪细胞内磷脂代谢。
Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/bi9870405
D Mann, A M Bersten
{"title":"Adrenaline stimulation of phospholipid metabolism in adipocyte ghosts.","authors":"D Mann,&nbsp;A M Bersten","doi":"10.1071/bi9870405","DOIUrl":"https://doi.org/10.1071/bi9870405","url":null,"abstract":"<p><p>The incorporation of long-chain fatty acids into phospholipids has been detected in adipocyte ghosts that were incubated with [1-14 C] stearic, [1-14 C] linoleic or [1-14 C] arachidonic acid. Adrenaline and adenosine activated this incorporation within 15 s of exposure of the ghosts to the hormones and the response was dose dependent. Maximum incorporation of labelled linoleic acid occurred at 10(-5) M adrenaline and 10(-7) M adenosine. The alpha-agonist phenylephrine and the beta-agonist isoproterenol were also shown to stimulate the incorporation of fatty acid in a dose dependent manner. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol were each labelled preferentially with linoleic or arachidonic acid. p-Bromophenacylbromide, quinacrine and centrophenoxine inhibited the adrenaline-stimulated incorporation of fatty acids into ghost membrane phospholipids, and p-bromophenacylbromide also reduced the activation of adenylate cyclase by adrenaline. NaF, an activator of adenylate cyclase, like adrenaline, stimulated the incorporation of linoleic acid into ghost membrane phospholipids.</p>","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14577705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Effects of coculture with uterine epithelial cells on the metabolism of glucose by mouse morulae and early blastocysts. 与子宫上皮细胞共培养对小鼠桑葚胚和早期囊胚葡萄糖代谢的影响。
Australian journal of biological sciences Pub Date : 1987-01-01 DOI: 10.1071/bi9870389
N K Khurana, R G Wales
{"title":"Effects of coculture with uterine epithelial cells on the metabolism of glucose by mouse morulae and early blastocysts.","authors":"N K Khurana,&nbsp;R G Wales","doi":"10.1071/bi9870389","DOIUrl":"https://doi.org/10.1071/bi9870389","url":null,"abstract":"<p><p>Coculture of mouse morulae/early blastocysts with isolated endometrial epithelial cells reduced incorporation of glucose carbon into embryonic glycogen but had no significant effect on incorporation into other internal carbon pools during a 5-h culture in serum-supplemented Dulbecco's modification of Eagle's minimum essential medium. Turnover of glycogen pools during 24-h chase culture of pulse-labelled embryos was unaffected by the presence of uterine epithelial cells recovered from day-4 pregnant or non-pregnant mice. However, significantly more label was retained in non-glycogen macromolecules during chase in the presence of endometrium recovered from non-pregnant than from pregnant uteri.</p>","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14577852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
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