Australian journal of biological sciences最新文献

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Insulin affects glucose uptake by muscle and mammary tissues of lactating ewes. 胰岛素影响泌乳母羊的肌肉和乳腺组织对葡萄糖的摄取。
Australian journal of biological sciences Pub Date : 1988-01-01 DOI: 10.1071/bi9880453
D Leenanuruksa, P Niumsup, G H McDowell
{"title":"Insulin affects glucose uptake by muscle and mammary tissues of lactating ewes.","authors":"D Leenanuruksa,&nbsp;P Niumsup,&nbsp;G H McDowell","doi":"10.1071/bi9880453","DOIUrl":"https://doi.org/10.1071/bi9880453","url":null,"abstract":"<p><p>Effects of insulin on exchanges of glucose across skeletal muscle and mammary tissue were measured in short-term studies in lactating ewes. Insulin secretion was suppressed by a primed/continuous infusion of somatostatin, then insulin was administered by continuous intravenous infusion of doses that were increased, in a step-wise manner, from 0 to 2 U h-1. Plasma glucose was maintained essentially constant by frequent monitoring and intravenous administration of exogenous glucose. Somatostatin suppressed but did not completely inhibit insulin secretion as shown by maintenance of plasma concentration of C-peptide. As plasma insulin was increased, while arterial glucose was maintained stable, uptake of glucose by skeletal muscle increased and glucose uptake by the mammary gland decreased. These observations confirm the role of insulin in regulating glucose uptake by skeletal muscle and raise the possibility that insulin also regulates glucose uptake by the mammary gland.</p>","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14043279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Controlled breeding in the Asiatic buffalo (Bubalus bubalis). 亚洲水牛(Bubalus bubalis)的控制繁殖。
I C Fletcher
{"title":"Controlled breeding in the Asiatic buffalo (Bubalus bubalis).","authors":"I C Fletcher","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Recent developments in exogenous hormone therapy to control and facilitate breeding in domestic buffalo cows (B. bubalis) are reviewed. Oestrus in domestic animals was synchronized satisfactorily during the normal breeding season by both of the standard treatments with prostaglandins or progestagens initially developed for use in Bos taurus cattle. Anoestrous cyclic cows treated with prostaglandin showed synchronized oestrus and conception rates similar to those recorded in normal cyclic animals, though the responses may have depended to some extent on increased intensity of observation of oestrus. Seasonally acyclic animals treated with progestagens and pregnant mare serum gonadotrophin also showed synchronized oestrus and conception rates equivalent to those recorded in cyclic animals, but these observations did not include prepubertal heifers or cows in the early stages of post-partum anoestrus. Controlled breeding did not overcome a general problem of low conception rates at spontaneous oestrus. Further investigations of controlled breeding should incorporate other management strategies, such as better feeding and reduced heat stress, which are known also to improve buffalo reproduction.</p>","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14399041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of anti-oestradiol-17B antibodies on the reproductive response of ewes superovulated with PMSG. 抗雌二醇- 17b抗体对PMSG超排卵母羊生殖反应的影响。
Australian journal of biological sciences Pub Date : 1988-01-01 DOI: 10.1071/bi9880441
J Eppleston, C D Nancarrow, K Battye, R M Hoskinson, E M Roberts
{"title":"Effect of anti-oestradiol-17B antibodies on the reproductive response of ewes superovulated with PMSG.","authors":"J Eppleston,&nbsp;C D Nancarrow,&nbsp;K Battye,&nbsp;R M Hoskinson,&nbsp;E M Roberts","doi":"10.1071/bi9880441","DOIUrl":"https://doi.org/10.1071/bi9880441","url":null,"abstract":"<p><p>A field experiment was conducted to examine the effect of anti-oestradiol-17B antibody titre on the oestrous and ovulatory responses of ewes to low (600 i.u.) or high (1200 i.u.) doses of pregnant mare's serum gonadotrophin (PMSG). Merino ewes were treated with intravaginal sponges and were subsequently used as vehicle-treated controls or were immunized to produce reciprocal anti-oestradiol-17B antibody titres less than 1000 or greater than 1000. Ewes were then treated with PMSG and the incidence of oestrus and ovulation, ovulation rate, and yield of embryos recorded. Treatment of immune ewes with 1200 i.u. PMSG resulted in both a higher proportion of ewes ovulating and a higher ovulation rate than in immune ewes treated with 600 i.u. (86% v. 67% and 13.4 v. 6.0 respectively). As anti-oestradiol-17B titres increased there was a reduction in the proportion of ewes exhibiting oestrus. The proportion of ewes ovulating decreased as antibody increased in ewes treated with 600 i.u. PMSG but not in those treated with 1200 i.u., suggesting an increased positive feedback of oestradiol with high PMSG doses. Fertilization rates were highest at the lower PMSG dose (68% v. 42%) and increased with increasing titre. Overall, there was no increase in ovulation rate or in yield of embryos over control values from either low (less than 1000) or high (greater than 1000) antibody titres.</p>","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14400556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polymorphic inversion and esterase loci complex on chromosome 2 of Drosophila buzzatii. II. Spatial variation. 雄果蝇2号染色体的多态性反转和酯酶位点复合体。2空间变异。
Australian journal of biological sciences Pub Date : 1988-01-01 DOI: 10.1071/bi9880239
W R Knibb, J S Barker
{"title":"Polymorphic inversion and esterase loci complex on chromosome 2 of Drosophila buzzatii. II. Spatial variation.","authors":"W R Knibb,&nbsp;J S Barker","doi":"10.1071/bi9880239","DOIUrl":"https://doi.org/10.1071/bi9880239","url":null,"abstract":"<p><p>The potential influence of linked inversions on allele frequency variation at the Est-1 and Est-2 loci among Australian populations of D. buzzatii was determined by statistical analyses allele and inversion gametic frequencies. Most of the significant spatial and climatic associations found for all Est-1 allele frequencies, and for one allele only of Est-2 (Est-2c+), were accounted for by their linkage disequilibria with the inversions, which covaried with environmental variables. Consistent with this result, the spatial and climatic associations for conditional Est-1 and Est-2 allele frequencies tended to be different from those for the respective unadjusted allele frequencies. In one important respect, the results for Est-1 and Est-2 were not altered by inversions. For both unadjusted and conditional Est-1 allele frequencies, few climatic associations remain after correcting for geographic location, whereas for both unadjusted and conditional Est-2 allele frequencies, climatic associations remain after correcting for geographic location. Thus, apparent selection affecting allele frequencies at the Est-2 locus is not accounted for by linked inversions.</p>","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14396171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Dietary regulation of ornithine transcarbamylase mRNA in liver and small intestine. 饲料对肝脏和小肠鸟氨酸转氨基甲酰基酶mRNA的调节作用。
Australian journal of biological sciences Pub Date : 1988-01-01 DOI: 10.1071/bi9880435
C Wraight, N Hoogenraad
{"title":"Dietary regulation of ornithine transcarbamylase mRNA in liver and small intestine.","authors":"C Wraight,&nbsp;N Hoogenraad","doi":"10.1071/bi9880435","DOIUrl":"https://doi.org/10.1071/bi9880435","url":null,"abstract":"<p><p>In the rat, changes in dietary protein intake give rise to changes in the levels of ornithine transcarbamylase (OTC) in liver and small intestine--an increase in liver and decrease in small intestine. The changes in enzyme level are accompanied by similar changes in levels of specific mRNA. Thus in liver, there is an increase in the level of specific mRNA when protein intake is increased, whereas in small intestine there is a small decrease. Comparison of changes in specific mRNA with total poly-A-containing RNA showed that the change in OTC mRNA in liver paralleled the change in total RNA levels. In contrast, in small intestine the small decrease in OTC mRNA levels when protein intake was increased was in the face of an increase in the level of total mRNA. Whereas the level of OTC is 20-fold higher in liver than in small intestine, the mRNA level for the enzyme differs by only 2.5-fold.</p>","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14400555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Immunofluorescent and Immunogold Localization of Glutamate Dehydrogenase in the Nodules of Soybean 大豆根瘤中谷氨酸脱氢酶的免疫荧光和免疫金定位
Australian journal of biological sciences Pub Date : 1988-01-01 DOI: 10.1071/BI9880475
Mc Wang, I. Kennedy
{"title":"Immunofluorescent and Immunogold Localization of Glutamate Dehydrogenase in the Nodules of Soybean","authors":"Mc Wang, I. Kennedy","doi":"10.1071/BI9880475","DOIUrl":"https://doi.org/10.1071/BI9880475","url":null,"abstract":"Soybean nodules have been examined for localization of glutamate dehydrogenase, using a monospecific antiserum to the plant enzyme prepared from lupin nodules. By immunofluorescence techniques, glutamate dehydrogenase was located in the cytoplasm at the periphery of infected cells adjacent to air spaces. A more precise localization within soybean-nodule mitochondria was shown by immuno-electron microscopy using protein A-gold labelling of thin sections.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81109304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Embryo manipulation in research and animal production. 研究和动物生产中的胚胎操作。
Australian journal of biological sciences Pub Date : 1988-01-01 DOI: 10.1071/BI9880117
J. Shelton
{"title":"Embryo manipulation in research and animal production.","authors":"J. Shelton","doi":"10.1071/BI9880117","DOIUrl":"https://doi.org/10.1071/BI9880117","url":null,"abstract":"Research in developmental biology has resulted in techniques to accelerate changes in gene frequency and to interfere directly in the genome. Procedures already in use or being adapted to livestock include embryo transfer, chimera production, embryo splitting, gene transfer and nuclear transplantation. Experiments with mouse embryos are revealing the principles governing embryonic development and differentiation and illustrate the need for these investigations to be extended to embryos of livestock. The optimal combination of these technologies in animal production strategies will depend upon further research and the role of animal products in society.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76156932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Influence of nutrition on ovulation rate in the ewe. 营养对母羊排卵率的影响。
Australian journal of biological sciences Pub Date : 1988-01-01 DOI: 10.1071/BI9880027
J. Smith
{"title":"Influence of nutrition on ovulation rate in the ewe.","authors":"J. Smith","doi":"10.1071/BI9880027","DOIUrl":"https://doi.org/10.1071/BI9880027","url":null,"abstract":"The effect of nutrition on ovulation rate in the ewe is reviewed with particular reference to the role of protein and energy and the time of effect during the cycle. Ovulation rate is increased by both protein and energy. In the case of protein this was shown to be accompanied by increased plasma levels of FSH and androstenedione at about the time of luteolysis, while levels of LH were unaffected. Increased hepatic oxidative enzyme activity is proposed as a mechanism by which nutrient intake may influence ovulation rate.","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79064345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 56
Control of glucose homeostasis in lactating ewes: use of the alloxan-diabetic/insulin-stabilized ewe to study effects of insulin and growth hormone. 控制泌乳母羊的葡萄糖稳态:使用四氧嘧啶糖尿病/胰岛素稳定母羊来研究胰岛素和生长激素的影响。
Australian journal of biological sciences Pub Date : 1988-01-01 DOI: 10.1071/bi9880421
D Leenanuruksa, G H McDowell
{"title":"Control of glucose homeostasis in lactating ewes: use of the alloxan-diabetic/insulin-stabilized ewe to study effects of insulin and growth hormone.","authors":"D Leenanuruksa,&nbsp;G H McDowell","doi":"10.1071/bi9880421","DOIUrl":"https://doi.org/10.1071/bi9880421","url":null,"abstract":"<p><p>Two separate experiments were conducted with alloxan-induced, diabetic ewes. In one study it was found that the diabetes induced by alloxan could be stabilized with exogenous insulin (1.2-1.3 U h-1). Feed intake and milk yield were maintained at normal levels even though a mild hyperglycaemia persisted. Despite this, milk fat content tended to increase, an observation that is consistent with insulin being a key factor in the aetiology of the low-milk-fat syndrome in the ruminant. Interruption of insulin infusion then resumption at 90% of the rate previously required to stabilize the diabetes was followed by marked changes in glucose kinetics. Initially, glucose production increased with little change in glucose utilization. This resulted in an increase in plasma glucose, which remained high even though both glucose production and utilization increased, to be similar on resumption of insulin infusions. It seems that the changed sensitivity to insulin reflects 'up-regulation' of insulin receptors. In a second study, exogenous recombinant bovine growth hormone (rebGH) was administered to insulin-stabilized, diabetic ewes. Immediately after the first injection of rebGH, glucose production increased with little change in glucose utilization, which led to increased plasma glucose. This observation suggests that rebGH was glucogenic. Ultimately, it was necessary to increase the dose of insulin to stabilize plasma glucose and by the fourth day of injection of rebGH, the insulin infusion rate required to stabilize the ewes had doubled from c. 1.5 to c. 3 U h-1. After cessation of injections of rebGH the dose of insulin required to stabilize the ewes decreased. These observations confirm the diabetogenic activity of growth hormone (GH) in the sheep.</p>","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14209987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Polyploid abnormalities in day 3 and day 5 merino sheep embryos. 第3天和第5天美利奴羊胚胎多倍体异常。
Australian journal of biological sciences Pub Date : 1988-01-01 DOI: 10.1071/bi9880157
J D Murray, C D Nancarrow, R J Scaramuzzi, Y Cognie
{"title":"Polyploid abnormalities in day 3 and day 5 merino sheep embryos.","authors":"J D Murray,&nbsp;C D Nancarrow,&nbsp;R J Scaramuzzi,&nbsp;Y Cognie","doi":"10.1071/bi9880157","DOIUrl":"https://doi.org/10.1071/bi9880157","url":null,"abstract":"<p><p>The chromosome complement was assessed in Merino sheep embryos collected at 3 and 5 days after the onset of oestrus. Donor ewe treatments were: untreated, or immunized against androstenedione (day 3); and untreated, or treated with follicle-stimulating hormone (FSH), or treated with FSH plus immunization against androstenedione (day 5). No significant differences in the frequency of chromosomally abnormal embryos between treatment groups within each age group were observed, so the data have been combined. Euploid abnormalities were observed in 10.8% of the day-3 embryos (4/37), with the abnormalities being one 1n, one 3n and two 5n. Embryos with euploidy (10%) were also observed at day 5, with three 1n/2n mosaics and a 3n embryo present in a sample of 40. These data suggest that chromosomally aberrant embryos are not lost before day 5 of development.</p>","PeriodicalId":8573,"journal":{"name":"Australian journal of biological sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1071/bi9880157","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14281458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
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