{"title":"Maternal Environmental Effects of Temperature and Exogenous Gibberellic Acid on Seed and Seedling Traits of Four Populations of Evening Primrose (Oenothera biennis)","authors":"Britanie M. LeFait, M. Qaderi","doi":"10.3390/seeds1020010","DOIUrl":"https://doi.org/10.3390/seeds1020010","url":null,"abstract":"Earlier studies have considered the separate effects of temperature and gibberellic acid (GA3) on plants and seeds. However, the combined effects of these factors on parent plants and their progeny have received little attention. We investigated the effects of two temperature regimes (24/20 °C and 28/24 °C, 16 h light/ 8 h dark) and two GA3 treatments (for two weeks) on the reproductive yield of parent plants, the subsequent seed germinability, and the seedling traits of four local populations of evening primrose (Oenothera biennis). Mature seeds were harvested and germinated, and seedlings were grown under the two temperature regimes. Parent plants were phenotyped for flower area and diameter, capsule length and width, full and empty capsule masses, and seed number and mass per capsule. Additionally, seed total germination and germination rate were determined, alongside stem height and dry mass, leaf number, area and dry mass, root dry mass, and total dry mass in seedlings. GA3 promoted the flowering of all populations in the first year. Maturation drying under higher temperatures resulted in more viable and faster germinating seeds. Higher GA3 did not affect total germination, but increased the germination rate of seeds that produced seedlings with lower total dry mass under the higher temperature regime. In conclusion, all populations responded similarly to GA3 treatment in terms of flowering, but responded differently to temperature during seed maturation, and subsequent seed germination and seedling growth.","PeriodicalId":85504,"journal":{"name":"Seeds (New York, N.Y.)","volume":"156 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78849024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shaila Shermin Tania, M. Rhaman, Farjana Rauf, Md. Moklasur Rahaman, M. Kabir, Md. Anamul Hoque, Y. Murata
{"title":"Alleviation of Salt-Inhibited Germination and Seedling Growth of Kidney Bean by Seed Priming and Exogenous Application of Salicylic Acid (SA) and Hydrogen Peroxide (H2O2)","authors":"Shaila Shermin Tania, M. Rhaman, Farjana Rauf, Md. Moklasur Rahaman, M. Kabir, Md. Anamul Hoque, Y. Murata","doi":"10.3390/seeds1020008","DOIUrl":"https://doi.org/10.3390/seeds1020008","url":null,"abstract":"Salinity is a dominant obstacle to the proper germination of seeds, growth of seedlings, and, consequently, the production of crops. The priming of seeds with different treating agents can efficiently impart salinity tolerance. Kidney bean is a nutritious and popular vegetable crop in the world. Literature shows that salt stress negatively disturbs the germination and growth of kidney beans. In the present research, we investigated the potentiality of salicylic acid (SA) and hydrogen peroxide (H2O2) as priming and exogenous agents to alleviate the salinity-inhibited germination and growth of kidney beans. The seeds were pretreated with SA (1 mM and 2 mM) and H2O2 (0.1 mM and 0.15 mM) and soaked in normal tap water (hydro-priming) for 60 min. In addition, for the control experiment, untreated seeds were used. Finally, primed seeds were subjected to salt stress (150 mM NaCl). Our results exhibited that salt stress considerably lowered the percentage of germination (GP), germination index (GI), seed vigor index (SVI), shoot length (SL), root length (RL), shoot–root fresh and dry biomass, and plant growth. The results also exhibited that salt stress significantly decreased the relative water content (RWC) and photosynthetic pigments such as chlorophyll, carotenoids, lycopene, and beta-carotene contents. The SA- and H2O2- and hydro-priming stimulated the GP, GI, SL, RL, SVI, and seedling growth. Data also revealed that the supplementation of SA and H2O2 enhanced RWC and photosynthetic pigments. When compared to other treatments, pretreatment with 1 mM SA was determined to be comparatively more effective at imparting the salt tolerance of kidney beans. Overall, these results, via a heatmap and principal component analysis, uncovered that priming and exogenous applications of SA and H2O2 can improve salt tolerance and enhance germination and seedling characteristics of kidney beans.","PeriodicalId":85504,"journal":{"name":"Seeds (New York, N.Y.)","volume":"502 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77057500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seed Priming Enhances Seed Germination and Morphological Traits of Lactuca sativa L. under Salt Stress","authors":"Bikash Adhikari, O. J. Olorunwa, T. C. Barickman","doi":"10.3390/seeds1020007","DOIUrl":"https://doi.org/10.3390/seeds1020007","url":null,"abstract":"Seed germination is the stage in which plants are most sensitive to abiotic stress, including salt stress (SS). SS affects plant growth and performance through ion toxicity, decreasing seed germination percentage and increasing the germination time. Several priming treatments were used to enhance germination under SS. The objectives of this study were (1) to identify priming treatments to shorten the emergence period, (2) to evaluate priming treatments against the SS, and (3) to induce synchronized seed germination. Salt-sensitive ‘Burpee Bibb’ lettuce seeds were treated with 0.05% potassium nitrate, 3 mM gibberellic acid, and distilled water. All the primed and non-primed seeds were subjected to 100 mM sodium chloride (NaCl) or 0 mM NaCl (control). The seven-day experiment, arranged in a complete randomized block design with four replications, was conducted in a growth chamber maintained with 16/8 h photoperiod (light/dark), 60% relative humidity, and a day/night temperature of 22/18 °C. The result indicated that hydro-primed (HP) seeds were better synchronized under SS. Similarly, fresh mass (FM) and dry mass (DM) of cotyledon, hypocotyl, and radicle were the highest in HP lettuce regardless of SS. Electrolyte leakage was the lowest in the HP lettuce, while other priming methods under SS increased membrane permeability, leading to osmotic stress and tissue damage. Overall, hydro-priming can be a good priming method for synchronizing germination and increasing FM and DM by creating the least osmotic stress and ion toxicity in lettuce under SS.","PeriodicalId":85504,"journal":{"name":"Seeds (New York, N.Y.)","volume":"107 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79222973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Bacherikov, Diana E. Raupova, A. Durova, Vladislav D. Bragin, E. Petrishchev, A. Novikov, D. Danilov, A. Zhigunov
{"title":"Coat Colour Grading of the Scots Pine Seeds Collected from Faraway Provenances Reveals a Different Germination Effect","authors":"I. Bacherikov, Diana E. Raupova, A. Durova, Vladislav D. Bragin, E. Petrishchev, A. Novikov, D. Danilov, A. Zhigunov","doi":"10.3390/seeds1010006","DOIUrl":"https://doi.org/10.3390/seeds1010006","url":null,"abstract":"The physiological quality of pine seeds is characterized by laboratory and field germination. The present paper is intended for technologists of seed plants and specialists of forest nurseries. It offers a solution to improve the seeding characteristics of small seeds by their pre-sowing preparation. The success of reforestation activities directly depends on the quality of the seeds. The influence of seed sorting by seed size and seed coat colour has been theoretically substantiated and repeatedly tested in practice. However, the response of seeds in germination can vary depending on the year and place of seed collection. Scots pine (Pinus sylvestris L.) seeds were germinated under controlled conditions. Seedlings were obtained from seeds pre-sorted by seed coat colour into white, brown, and black groups, and further divided by size. The results of sorting by the colour of seed coat indicate a different effect of this pre-sowing treatment on the sowing qualities of seeds. Brown seed coat colour showed the highest percentage degree in the seedlots of all provenances. The seeds from the southern provenance with the brown seed coat colour shown the maximum germination. That said, the study raises new questions, indicating more comprehensive research in the future. Does the pattern of germination parameter distribution remain constant for seeds of other harvest years but of the same provenance? Does the variability of the germination factor the result of internal factors of the container location in the greenhouse? Is the genetic diversity of seedlings disturbed by sorting by size?","PeriodicalId":85504,"journal":{"name":"Seeds (New York, N.Y.)","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73349440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Naeimeh Sousaraei, B. Torabi, E. Soltani, K. Mashayekhi, J. Medina
{"title":"Differential Seed Germination Responses of Tomato Landraces to Temperature under Climate Change Scenarios","authors":"Naeimeh Sousaraei, B. Torabi, E. Soltani, K. Mashayekhi, J. Medina","doi":"10.3390/seeds1010005","DOIUrl":"https://doi.org/10.3390/seeds1010005","url":null,"abstract":"This study aims to quantify the seed germination response of six tomato landraces to temperature; predict future climate changes relative to the baseline period (1980–2009) for studied locations in the courses of near-term (2010–2039) and mid-term (2040–2069) under two representative concentration pathways (RCP 4.5 and 8.5); assess the impact of future climate change on the final germination percentage (FGP) and time to reach 50% germination (D50) in the studied landraces. The results show that FGP is zero at 10 °C, reaches the highest value at 15–35 °C, and ceases at 36–40 °C for all landraces. The results also demonstrate that the temperature increment is 0.8–1.3 °C and 1.5–2.7 °C in the near- and mid-term, respectively, under RCP 4.5; further, this increment is 0.9–1.9 °C for the near-term and 2.3–3.4 °C for the mid-term under RCP 8.5. It estimates that the D50 takes 2.5 to 3.8 days among the locations in the baseline course. In the near term, the D50 would be 2.2 to 3.4 days under RCP 4.5 and 2.1 to 3.3 days under RCP 8.5. For the mid-term, the D50 would be projected between 1.9 and 2.9 days under RCP 4.5 and 1.8 to 2.7 days under RCP 8.5. The FGP increases up to 19.5% for Gorgan and 21.3% for Varamin under climatic scenarios relative to baseline, and it will not change in the future climate for other landraces. In conclusion, global warming can result in rapid, uniform, and complete germination in different tomato landraces.","PeriodicalId":85504,"journal":{"name":"Seeds (New York, N.Y.)","volume":"135 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73423295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Fagundes, H. T. Dos Santos, P. Cuevas‐Reyes, T. Cornelissen
{"title":"Biotic and Abiotic Interactions Shape Seed Germination of a Fire-Prone Species","authors":"M. Fagundes, H. T. Dos Santos, P. Cuevas‐Reyes, T. Cornelissen","doi":"10.3390/seeds1010003","DOIUrl":"https://doi.org/10.3390/seeds1010003","url":null,"abstract":"Both biotic and abiotic environmental filters drive the occurrence, distribution, and persistence of plant species. Amongst drivers that influence the distribution of plants in harsh environments, seed predation and temperature are particularly important in habitats that are prone to fire. In this study, we highlight the combined effects of predation and high temperature simulating fire to understand its effects on the germination percentage and germination speed of the fire prone species Copaifera oblongifolia. Groups of seeds attacked by the beetles Rhinochenus brevicollis and Apion sp., seeds manipulated by the ant Atta laevigata, and seeds left intact were put to germinate in controlled environments. To evaluate the effects of abiotic filters, seeds with intact elaiosomes and seeds with elaiosomes removed by the ant Atta laevigata were exposed to temperatures of 27, 60, 100, and 200 °C. The results showed that only 2.8% of the seeds attacked by R. brevicollis germinated. Seeds attacked by Apion sp. germinated faster, followed by seeds with their elaiosomes removed and seeds with intact elaiosomes. Seeds attacked by Apion sp. had the lowest germination percentage. The temperature of 200 °C killed seed embryos, whereas seeds exposed to 100 °C took longer to germinate than seeds exposed to other temperatures. Our results reveal that fire intensity and seed damage are important drivers of seed germination of C. oblongifolia.","PeriodicalId":85504,"journal":{"name":"Seeds (New York, N.Y.)","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76042327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Hernández, P. Díaz‐Vivancos, J. Acosta-Motos, G. Barba-Espín
{"title":"Potassium Nitrate Treatment Is Associated with Modulation of Seed Water Uptake, Antioxidative Metabolism and Phytohormone Levels of Pea Seedlings","authors":"J. Hernández, P. Díaz‐Vivancos, J. Acosta-Motos, G. Barba-Espín","doi":"10.3390/seeds1010002","DOIUrl":"https://doi.org/10.3390/seeds1010002","url":null,"abstract":"(1) Background: Seed treatment with potassium nitrate (KNO3) has been associated with dormancy breaking, improved germination and enhanced seedling growth and uniformity in a variety of plant species. However, the KNO3 effect seems to be dependent on plant species and treatment conditions. (2) Methods: We describe the effect of incubation of dry pea seeds with different KNO3 concentration on water uptake kinetic, early seedling growth, antioxidant metabolism and hormone profile in pea seedlings. (3) Results: Low (0.25 mM) KNO3 levels increased seedling water uptake and growth, whereas high (40 mM) levels decreased seedling growth. KNO3 treatment differentially affected the antioxidant defences. Low KNO3 levels maintained the activity of antioxidant enzymes, while high levels reduced the activity of H2O2-scavenging enzymes. KNO3 induced a progressive decline in ascorbate levels and reduced (GSH) and oxidised (GSSG) glutathione. Low KNO3 levels strongly increased GA1 and decreased ABA in both seedlings and cotyledons, resulting in a decline in the ABA/GAs ratio. (4) Conclusions: Pea seed treatment with a low KNO3 level promoted early seedling growth. In this process, an interaction among KNO3, antioxidant defences and ABA/GAs ratio is proposed.","PeriodicalId":85504,"journal":{"name":"Seeds (New York, N.Y.)","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75427598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seed Science Research: Global Trends in Seed Biology and Technology","authors":"José Antonio Hernández Cortés","doi":"10.3390/seeds1010001","DOIUrl":"https://doi.org/10.3390/seeds1010001","url":null,"abstract":"Seeds are the central components of the plant life cycle because the establishment of a new generation of plants depends on them [...]","PeriodicalId":85504,"journal":{"name":"Seeds (New York, N.Y.)","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78856928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seeds","authors":"Mel Pennant","doi":"10.5040/9781784606152.00000002","DOIUrl":"https://doi.org/10.5040/9781784606152.00000002","url":null,"abstract":"Enochs and Riggs (1990) developed the Teachers’ Efficacy and Beliefs Instrument (TEBI) to evaluate educators’ judgements about their ability to evoke student success; however, there has been inadequate psychometrics conducted on this instrumentation. This study provides a psychometrically sound instrument development and construct validation process for our modified version of TEBI. The qualitative data indicated that item revisions were required before quantitative data collection. The modified TEBI measure will inform the curriculum Pre-K STEM, nutrition, and literacy programming for an NIH-funded grant.","PeriodicalId":85504,"journal":{"name":"Seeds (New York, N.Y.)","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86192311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Forest conservation in Nepal: encouraging women's participation.","authors":"A Molnar","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":85504,"journal":{"name":"Seeds (New York, N.Y.)","volume":" 10","pages":"1-20"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22012583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}