Alleviation of Salt-Inhibited Germination and Seedling Growth of Kidney Bean by Seed Priming and Exogenous Application of Salicylic Acid (SA) and Hydrogen Peroxide (H2O2)

Seeds (New York, N.Y.) Pub Date : 2022-04-08 DOI:10.3390/seeds1020008

Shaila Shermin Tania, M. Rhaman, Farjana Rauf, Md. Moklasur Rahaman, M. Kabir, Md. Anamul Hoque, Y. Murata

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引用次数: 9

Abstract

Salinity is a dominant obstacle to the proper germination of seeds, growth of seedlings, and, consequently, the production of crops. The priming of seeds with different treating agents can efficiently impart salinity tolerance. Kidney bean is a nutritious and popular vegetable crop in the world. Literature shows that salt stress negatively disturbs the germination and growth of kidney beans. In the present research, we investigated the potentiality of salicylic acid (SA) and hydrogen peroxide (H2O2) as priming and exogenous agents to alleviate the salinity-inhibited germination and growth of kidney beans. The seeds were pretreated with SA (1 mM and 2 mM) and H2O2 (0.1 mM and 0.15 mM) and soaked in normal tap water (hydro-priming) for 60 min. In addition, for the control experiment, untreated seeds were used. Finally, primed seeds were subjected to salt stress (150 mM NaCl). Our results exhibited that salt stress considerably lowered the percentage of germination (GP), germination index (GI), seed vigor index (SVI), shoot length (SL), root length (RL), shoot–root fresh and dry biomass, and plant growth. The results also exhibited that salt stress significantly decreased the relative water content (RWC) and photosynthetic pigments such as chlorophyll, carotenoids, lycopene, and beta-carotene contents. The SA- and H2O2- and hydro-priming stimulated the GP, GI, SL, RL, SVI, and seedling growth. Data also revealed that the supplementation of SA and H2O2 enhanced RWC and photosynthetic pigments. When compared to other treatments, pretreatment with 1 mM SA was determined to be comparatively more effective at imparting the salt tolerance of kidney beans. Overall, these results, via a heatmap and principal component analysis, uncovered that priming and exogenous applications of SA and H2O2 can improve salt tolerance and enhance germination and seedling characteristics of kidney beans.

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水杨酸(SA)和过氧化氢(H2O2)缓解盐对芸豆萌发和幼苗生长的抑制作用

盐度是影响种子正常发芽、幼苗生长，进而影响作物生产的主要障碍。用不同的处理剂灌种可以有效地提高种子的耐盐性。芸豆是一种营养丰富、深受世界各国欢迎的蔬菜作物。文献表明，盐胁迫对芸豆的萌发和生长有不利影响。在本研究中，我们研究了水杨酸(SA)和过氧化氢(H2O2)作为引发剂和外源剂对缓解咸水抑制芸豆发芽和生长的潜力。分别用SA (1 mM和2 mM)和H2O2 (0.1 mM和0.15 mM)对种子进行预处理，用普通自来水(水浸)浸泡60 min。另外，对照实验采用未经处理的种子。最后，进行盐胁迫(150 mM NaCl)处理。结果表明，盐胁迫显著降低了油菜的发芽率(GP)、发芽指数(GI)、种子活力指数(SVI)、茎长(SL)、根长(RL)、茎根鲜干生物量和植株生长。结果还表明，盐胁迫显著降低了相对含水量(RWC)和叶绿素、类胡萝卜素、番茄红素和β -胡萝卜素等光合色素含量。SA-、H2O2-和氢激发刺激了GP、GI、SL、RL、SVI和幼苗的生长。此外，SA和H2O2的添加也提高了RWC和光合色素的含量。与其他处理相比，1 mM SA预处理在提高芸豆耐盐性方面相对更有效。总的来说，通过热图和主成分分析，这些结果揭示了SA和H2O2的激发和外源施用可以提高芸豆的耐盐性，提高发芽和幼苗特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Seeds (New York, N.Y.)

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