Laboratory hematology : official publication of the International Society for Laboratory Hematology最新文献_第4页

Identification and selective depletion of alloreactive T-cells for adoptive immunotherapy. 过继免疫治疗中同种异体反应性t细胞的鉴定和选择性耗竭。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2010-03-01 DOI: 10.1532/LH96.09010

E Rettinger, M Schumm, M Pfeiffer, S Kuçi, A Willasch, R Handgretinger, D Niethammer, P Lang

{"title":"Identification and selective depletion of alloreactive T-cells for adoptive immunotherapy.","authors":"E Rettinger, M Schumm, M Pfeiffer, S Kuçi, A Willasch, R Handgretinger, D Niethammer, P Lang","doi":"10.1532/LH96.09010","DOIUrl":"https://doi.org/10.1532/LH96.09010","url":null,"abstract":"Background: T-cell-depleted allografts may exhibit delayed T-cell recovery, severe infections, and relapse after haploidentical hematopoietic stem cell transplantation (HSCT). Required donor lymphocyte infusions containing nonalloreactive cells may transfer immune function without causing graft-versus-host disease.Methods: We developed an ex vivo approach for the immunomagnetic depletion of alloreactive CD25+, CD69+, and HLA-DR+ T-cells. To achieve highest rates of alloantigen expression, we cocultured peripheral blood mononuclear cells (PBMNCs) with PBMNCs (A/B*), dendritic cells (A/B* DCs), or cytokine-pretreated PBMNCs (A/B* cyt cells). Functional analyses were performed after depletion.Results: After coculture with PBMNCs (A/B* cells), 29% of T-cells became CD25+, CD69+, and HLA-DR+. In modified mixed lymphocyte reactions (MLR) (A/B* cyt cells and A/B* DCs), 35% and 37% of T-cells became CD25+, CD69+, and HLA-DR+. Alloactivation was confirmed by interferon gamma release and proliferation. Immuno-magnetic depletion produced <1% alloactivated cells. Furthermore, this depletion strategy was allospecific and hardly impaired the immune function of the retained cells.Discussion: The efficiency of immunomagnetic depletion depended on the stimulatory capacity of stimulator cells and was improved by using cytokine-pretreated PBMNCs for alloactivation. Overall, this approach might be a promising strategy for restoration of the immune system, particularly after haploidentical HSCT.","PeriodicalId":85078,"journal":{"name":"Laboratory hematology : official publication of the International Society for Laboratory Hematology","volume":"16 1","pages":"8-22"},"PeriodicalIF":0.0,"publicationDate":"2010-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28771883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Time to drop routine reporting of differential percentage values from CBC reports. 是时候放弃常规报告CBC报告的差异百分比值了。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2010-03-01 DOI: 10.1532/LH96.09017

David L Zwick

引用次数: 0

Book Review: Comments on the 2008 WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues by a Practicing Hematopathologist 书评:一位执业血液病理学家对2008年WHO《造血和淋巴组织肿瘤分类》的评论

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2009-06-01 DOI: 10.1532/LH96.09006

G. Shaw

引用次数: 0

Ethnic and genetic causes of neutropenia: clinical and therapeutic implications. 中性粒细胞减少症的种族和遗传原因:临床和治疗意义。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2009-01-01 DOI: 10.1532/LH96.09005

Guido D'Angelo

{"title":"Ethnic and genetic causes of neutropenia: clinical and therapeutic implications.","authors":"Guido D'Angelo","doi":"10.1532/LH96.09005","DOIUrl":"https://doi.org/10.1532/LH96.09005","url":null,"abstract":"The white blood cell count represents clinical data linked with different pathologic conditions, as well as with lifestyle. Another very important condition that affects the number of leukocytes is race and ethnic group-the geographic zone of origin. Genetic studies have identified the gene that controls the expression of the Duffy antigen receptor for chemokine (DARC), which is associated with the ethnic group to which individuals belong. The single-nucleotide polymorphism strongly associated with race is DARC rs2814778. Currently, it is the only condition that can explain the difference in white blood cell count between different ethnic groups. In a society increasingly characterized by multiracial issues, the leukopenia and/or neutropenia in ethnic groups must be known and accurately assessed clinically. Improved knowledge of this association may help in optimizing therapeutic approaches, mainly for African patients with severe diseases, cancer in particular. Recently, preclinical data have also suggested a link between the Duffy antigen and coagulation. This review also discusses the main causes of genetic neutropenia.","PeriodicalId":85078,"journal":{"name":"Laboratory hematology : official publication of the International Society for Laboratory Hematology","volume":"15 3","pages":"25-9"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28479207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The coinheritance of beta- and alpha- thalassemia: a review of one patient and her family. -和-地中海贫血的共遗传:一个病人和她的家庭的回顾。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2009-01-01 DOI: 10.1532/LH96.09002

Kelley J Mast, Sue Hammond, Stephen J Qualman, Samir B Kahwash

引用次数: 2

Aberrant phenotypic expression of the T-cell-associated antigen CD8 on B-cell chronic lymphocytic leukemia cells. t细胞相关抗原CD8在b细胞慢性淋巴细胞白血病细胞上的异常表型表达。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2009-01-01 DOI: 10.1532/LH96.08015

Fabio Stagno, Anna Triolo, Vittorio Del Fabro, Salvatore Berretta, Nunziatina Laura Parrinello, Giovannella Fargione, Francesco Di Raimondo

引用次数: 6

Compound heterozygosity for hemoglobin S [beta6(A3)Glu6Val] and hemoglobin Korle-Bu [beta73(E17)Asp73Asn]. 血红蛋白S [beta6(A3)Glu6Val]和血红蛋白Korle-Bu [beta73(E17)Asp73Asn]的复合杂合性。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2009-01-01 DOI: 10.1532/LH96.09004

Pascale S Akl, Ferdane Kutlar, Niren Patel, Catherine L Salisbury, Peter Lane, Andrew N Young

引用次数: 6

False-positive crystals in urine from contamination by Multistix 10 SG reagent strips for urinalysis. 尿液分析用Multistix 10sg试剂条污染导致尿液结晶假阳性。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2009-01-01 DOI: 10.1532/LH96.09008

Ji Yeon Kim, Svetal Patel, Anand S Dighe

引用次数: 0

The association of D-dimer levels with clinical outcomes in patients presenting with acute pulmonary embolism. d -二聚体水平与急性肺栓塞患者临床结果的关系

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2009-01-01 DOI: 10.1532/LH96.08014

John Blamoun, Maria Alfakir, Ashraf I R Sedfawy, Mahmoud Q Moammar, Michael Maroules, M Anees Khan, Vincent A DeBari

{"title":"The association of D-dimer levels with clinical outcomes in patients presenting with acute pulmonary embolism.","authors":"John Blamoun, Maria Alfakir, Ashraf I R Sedfawy, Mahmoud Q Moammar, Michael Maroules, M Anees Khan, Vincent A DeBari","doi":"10.1532/LH96.08014","DOIUrl":"https://doi.org/10.1532/LH96.08014","url":null,"abstract":"The D-dimer fragment of fibrin degradation has been a useful adjunct in the diagnosis of venous thromboembolism (VTE). In conjunction with predictive algorithms, the high negative predictive value (NPV) of D-dimer measurements has provided this analyte with a prominent position in the diagnosis of pulmonary embolism (PE). The purpose of this study was to determine if D-dimer levels correlate with ventilation/perfusion (V/Q) derangements as assessed by the alveolar-arterial oxygen tension gradient (DeltaA-a) and to ascertain if quantitative measurements of D-dimer on admission have prognostic value in terms of during-admission mortality and recurrence over a 60-week period. The study utilized a retrospective cohort of 108 subjects admitted to a single institution and studied longitudinally. The cohort was divided into 4 groups representing degree of severity assessed by computed tomographic (CT) angiography: mild, moderate, severe, and very severe. Differences in D-dimer levels among these groups were strongly significant (P < .0001). A strong correlation was observed between D-dimer concentration and DeltaA-a (P < .0001). Logistic methods were used to calculate a \"cut-off\" level that would distinguish mild-moderate from severe-very severe PE. At a concentration of 12.35 mug/mL, this level yielded an odds ratio (OR) of 12.64 (P = .006) for during-admission mortality and a hazard ratio (HR) of 0.13 (P < .0001) for 60-week recurrence. These data suggest that D-dimer levels have utility beyond their NPV and should be considered as potential prognostic markers in subjects presenting with acute PE.","PeriodicalId":85078,"journal":{"name":"Laboratory hematology : official publication of the International Society for Laboratory Hematology","volume":"15 1","pages":"4-9"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28032636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

An effective method for hemoglobin E detection: DEAE sepharose microcolumn. 一种检测血红蛋白E的有效方法:DEAE磷酸酯微柱。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2009-01-01 DOI: 10.1532/LH96.08011

Orathai Tangvarasittichai, Surapon Tangvarasittichai

引用次数: 1