Journal of Zhejiang University. Science最新文献_第7页

Immunotherapy of intracranial G422 glioblastoma with dendritic cells pulsed with tumor extract or RNA. 树突状细胞与肿瘤提取物或RNA脉冲免疫治疗颅内G422胶质母细胞瘤。

Journal of Zhejiang University. Science Pub Date : 2004-10-01 DOI: 10.1631/jzus.2004.1298

Zhe Zhang, Ling-ling Tang, Ren-ya Zhan, Ying Tong, Hang-ping Yao, Li-an Du

{"title":"Immunotherapy of intracranial G422 glioblastoma with dendritic cells pulsed with tumor extract or RNA.","authors":"Zhe Zhang, Ling-ling Tang, Ren-ya Zhan, Ying Tong, Hang-ping Yao, Li-an Du","doi":"10.1631/jzus.2004.1298","DOIUrl":"https://doi.org/10.1631/jzus.2004.1298","url":null,"abstract":"Objective: To investigate the anti-tumor efficacy of dendritic cell (DC)-based vaccines pulsed with tumor extracts or RNA in a mouse model of intracranial G422 glioblastoma.Methods: Bone marrow-derived DCs were pulsed ex vivo with tumor extracts or RNA. Ninety female mice harboring 4-day-old intracranial G422 glioblastomas and 126 normal mice were treated with three spaced one week apart subcutaneous injections either with PBS, unpulsed DCs, G422 tumor extracts, RNA, DCs pulsed with G422 tumor extracts (DC/extract) or with RNA (DC/RNA). Seven days after the third immunization of normal mice, the spleens of 36 of them were harvested for cytotoxic T lyphocyte (CTL) assays and the others were challenged in the brain with G422 tumor cells. All the treated mice were followed for survival. Some mice brains were removed and examined pathologically when they died.Results: Immunization using DC/extract or DC/RNA significantly induced G422-specific CTL responses compared with control groups (P<0.01). Vaccination with DC/extract or DC/RNA, either prior to G422 tumor challenge or in tumor-harboring mice, significantly prolonged survival compared with other control groups (P<0.01).Conclusion: DCs pulsed with tumor extracts or RNA derived from autologous tumors has potential antitumor effects via activation of cell-mediated immunity. Our results suggest a useful therapeutic strategy against gliomas.","PeriodicalId":85042,"journal":{"name":"Journal of Zhejiang University. Science","volume":"5 10","pages":"1298-303"},"PeriodicalIF":0.0,"publicationDate":"2004-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1631/jzus.2004.1298","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24678526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Ultrastructure of spermatozoa of Bullacta exarata (philippi) and its significance on reproductive evolution and physio-ecological adaptation. 黄颡鱼精子超微结构及其在生殖进化和生理生态适应中的意义。

Journal of Zhejiang University. Science Pub Date : 2004-10-01 DOI: 10.1631/jzus.2004.1211

Xue-ping Ying, Wan-xi Yang, Nai-cheng Jiang, Yong-pu Zhang

引用次数: 13

Protein folding pathology in domestic animals. 家畜的蛋白质折叠病理学。

Journal of Zhejiang University. Science Pub Date : 2004-10-01 DOI: 10.1631/jzus.2004.1226

Erik Gruys

{"title":"Protein folding pathology in domestic animals.","authors":"Erik Gruys","doi":"10.1631/jzus.2004.1226","DOIUrl":"10.1631/jzus.2004.1226","url":null,"abstract":"Fibrillar proteins form structural elements of cells and the extracellular matrix. Pathological lesions of fibrillar microanatomical structures, or secondary fibrillar changes in globular proteins are well known. A special group concerns histologically amorphous deposits, amyloid. The major characteristics of amyloid are: apple green birefringence after Congo red staining of histological sections, and non-branching 7-10 nm thick fibrils on electron microscopy revealing a high content of cross beta pleated sheets. About 25 different types of amyloid have been characterised. In animals, AA-amyloid is the most frequent type. Other types of amyloid in animals represent: AIAPP (in cats), AApoAI, AApoAII, localised AL-amyloid, amyloid in odontogenic or mammary tumors and amyloid in the brain. In old dogs Abeta and in sheep APrPsc-amyloid can be encountered. AA-amyloidosis is a systemic disorder with a precursor in blood, acute phase serum amyloid A (SAA). In chronic inflammatory processes AA-amyloid can be deposited. A rapid crystallization of SAA to amyloid fibrils on small beta-sheeted fragments, the 'amyloid enhancing factor' (AEF), is known and the AEF has been shown to penetrate the enteric barrier. Amyloid fibrils can aggregate from various precursor proteins in vitro in particular at acidic pH and when proteolytic fragments are formed. Molecular chaperones influence this process. Tissue data point to amyloid fibrillogenesis in lysosomes and near cell surfaces. A comparison can be made of the fibrillogenesis in prion diseases and in enhanced AA-amyloidosis. In the reactive form, acute phase SAA is the supply of the precursor protein, whereas in the prion diseases, cell membrane proteins form a structural source. Abeta-amyloid in brain tissue of aged dogs showing signs of dementia forms a canine counterpart of senile dementia of the Alzheimer type (ccSDAT) in man. Misfolded proteins remain potential food hazards. Developments concerning prevention of amyloidogenesis and therapy of amyloid deposits are shortly commented.","PeriodicalId":85042,"journal":{"name":"Journal of Zhejiang University. Science","volume":"5 10","pages":"1226-38"},"PeriodicalIF":0.0,"publicationDate":"2004-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1388739/pdf/JZUS05-1226.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24676864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of embryonic chromosomal abnormality using FISH-based preimplantation genetic diagnosis. 利用基于fish的胚胎着床前遗传学诊断鉴定胚胎染色体异常。

Journal of Zhejiang University. Science Pub Date : 2004-10-01 DOI: 10.1631/jzus.2004.1249

Ying-hui Ye, Chen-ming Xu, Fan Jin, Yu-li Qian

{"title":"Identification of embryonic chromosomal abnormality using FISH-based preimplantation genetic diagnosis.","authors":"Ying-hui Ye, Chen-ming Xu, Fan Jin, Yu-li Qian","doi":"10.1631/jzus.2004.1249","DOIUrl":"https://doi.org/10.1631/jzus.2004.1249","url":null,"abstract":"Objective: Embryonic chromosomal abnormality is one of the main reasons for in vitro fertilization (IVF) failure. This study aimed at evaluating the value of Fluorescence in-situ Hybridization (FISH)-based Preimplantation Genetic Diagnosis (PGD) in screening for embryonic chromosomal abnormality to increase the successful rate of IVF.Method: Ten couples, four with high risk of chromosomal abnormality and six infertile couples, underwent FISH-based PGD during IVF procedure. At day 3, one or two blastomeres were aspirated from each embryo. Biopsied blastomeres were examined using FISH analysis to screen out embryos with chromosomal abnormalities. At day 4, embryos without detectable chromosomal abnormality were transferred to the mother bodies as in regular IVF.Results: Among 54 embryos screened using FISH-based PGD, 30 embryos were detected to have chromosomal abnormalities. The 24 healthy embryos were implanted, resulting in four clinical pregnancies, two of which led to successful normal birth of two healthy babies; one to ongoing pregnancy during the writing of this article; and one to ectopic pregnancy.Conclusion: FISH-based PGD is an effective method for detecting embryonic chromosomal abnormality, which is one of the common causes of spontaneous miscarriages and chromosomally unbalanced offsprings.","PeriodicalId":85042,"journal":{"name":"Journal of Zhejiang University. Science","volume":"5 10","pages":"1249-54"},"PeriodicalIF":0.0,"publicationDate":"2004-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1631/jzus.2004.1249","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24678519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Time domain system identification of unknown initial conditions. 未知初始条件的时域系统辨识。

Journal of Zhejiang University. Science Pub Date : 2004-09-01 DOI: 10.1631/jzus.2004.1035

Wen-pei Sung, Vernon C Matzen, Ming-hsiang Shih

引用次数: 4

Congestion control for ATM multiplexers using neural networks: multiple sources/single buffer scenario. 使用神经网络的ATM多路复用器拥塞控制:多源/单缓冲方案。

Journal of Zhejiang University. Science Pub Date : 2004-09-01 DOI: 10.1631/jzus.2004.1124

Shu-xin Du, Shi-yong Yuan

引用次数: 2

Pneumatic active suspension system for a one-wheel car model using fuzzy reasoning and a disturbance observer. 基于模糊推理和扰动观测器的单轮汽车气动主动悬架系统模型。

Journal of Zhejiang University. Science Pub Date : 2004-09-01 DOI: 10.1631/jzus.2004.1060

Toshio Yoshimura, Atsushi Takagi

引用次数: 23

Which is better for presenting your data: table or graph? 表和图哪一个更适合展示你的数据?

Journal of Zhejiang University. Science Pub Date : 2004-09-01 DOI: 10.1631/jzus.2004.1165

Li Zhang, Xian-lan Fu

引用次数: 1

On service differentiation in mobile Ad Hoc networks. 移动Ad Hoc网络中的业务差异化研究。

Journal of Zhejiang University. Science Pub Date : 2004-09-01 DOI: 10.1631/jzus.2004.1087

Shun-liang Zhang, Cheng-qing Ye

引用次数: 2

Optimal parallel algorithm for shortest paths problem on interval graphs. 区间图上最短路径问题的最优并行算法。

Journal of Zhejiang University. Science Pub Date : 2004-09-01 DOI: 10.1631/jzus.2004.1135

P K Mishra

引用次数: 0