Amanda R. McGettigan, Daniel Kwasniewski, R. Chinni
{"title":"DETECTION OF MERCURY IN NATURAL WATERS IN BERKS COUNTY, PENNSYLVANIA, USING COLD VAPOR ATOMIC ABSORPTION SPECTROSCOPY","authors":"Amanda R. McGettigan, Daniel Kwasniewski, R. Chinni","doi":"10.5325/jpennacadscie.86.1.0054","DOIUrl":"https://doi.org/10.5325/jpennacadscie.86.1.0054","url":null,"abstract":"\u0000 Cold Vapor Atomic Absorption Spectroscopy (CVAAS) was used to detect potential mercury contamination in sixteen natural waterways in Berks County, Pennsylvania. Four samples were collected from each site in mercury-free Nalgene containers. After collection, the samples were brought back to the lab, acidified, and refrigerated until analysis could proceed. For analysis, each sample was prepared by adding various reagents and by digesting each in a 95°C water bath for approximately two hours. The digested samples were analyzed using tin (II) chloride and the maximum absorbance of each sample was recorded. Calibration curves were developed using serial dilutions of the mercury standard; these curves allowed for calculation of detection limits for mercury and were also used to calculate the mercury concentration in the samples if any was present. The results showed that none of the waterways tested had significant amounts of mercury present.","PeriodicalId":85037,"journal":{"name":"Journal of the Pennsylvania Academy of Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5325/jpennacadscie.86.1.0054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70852902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"LIGHT AND SCANNING ELECTRON MICROSCOPIC OBSERVATIONS OF THE CERCARIAE AND REDIAE OF RIBEIROIA ONDATRAE","authors":"Shamus P. Keeler, B. Fried, J. Huffman","doi":"10.5325/jpennacadscie.86.1.0030","DOIUrl":"https://doi.org/10.5325/jpennacadscie.86.1.0030","url":null,"abstract":"\u0000 Ribeiroia ondatrae (Price, 1931) is a digenetic trematode occurring in lentic aquatic systems throughout North and South America and has been identified as a cause of limb malformations in numerous species of amphibians. Several questions still remain related to basic morphology of the various life stages of R. ondotrae. The purpose of this study was to describe the daughter rediae and cercariae of R. ondatrae using light and scanning electron microscopy. Helisoma trivolvis snails infected with R. ondatrae were collected from Delaware Lake, NJ. Daughter rediae and cercariae were collected from the infected snails, and light and scanning electron microscopy were used to describe the life stages with particular focus on the tegumentary surface. The tegument of R. ondatrae rediae and cercariae is aspinose despite reports in the literature describing a spinose tegument in the cercariae. The rediae and cercariae were observed to be much simpler in tegumentary structure than various species of Echinostoma that have been studied recently with scanning electron microscopy.","PeriodicalId":85037,"journal":{"name":"Journal of the Pennsylvania Academy of Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70852662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shamus P. Keeler, Kelcey I. Burguess, Lemaster Heather, J. Huffman
{"title":"STAPHYLOCOCCUS INTERMEDIUS DERMATITIS IN DENNING NEW JERSEY BLACK BEARS (Ursus americanus)","authors":"Shamus P. Keeler, Kelcey I. Burguess, Lemaster Heather, J. Huffman","doi":"10.5325/jpennacadscie.86.1.0075","DOIUrl":"https://doi.org/10.5325/jpennacadscie.86.1.0075","url":null,"abstract":"\u0000 On 18 March 2006, during annual den research, personnel from the New Jersey Division of Fish and Wildlife Black Bear Project examined a 5-yr-old female and three yearling black bears (Ursus americanus) with severe dermatitis. The female and three yearlings all exhibited weight loss. Deep skin scrapings were taken and examined under a stereomicroscope. The skin of each bear was swabbed with BBL CultureSwabs. No mites were found in the skin scrapings. Staphylococcus intermedius was the only bacterial species isolated from the four bears. To our knowledge this is the first report of non-mange related dermatitis caused by S. intermedius in black bears.\u0000 New Jersey black bears (Ursus americanus) are found primarily in the northern portion of the state but the population has been steadily moving south. The average New Jersey black bear litter size is 2.7 cubs. They den in rock cavities, brush piles, felled trees, and open nests (Carr and Burguess, 2004).","PeriodicalId":85037,"journal":{"name":"Journal of the Pennsylvania Academy of Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70853142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Francl, Tessa K. Canniff, Bland R. CRAIG, D. Sparks, V. Brack
{"title":"QUANTIFYING WING DAMAGE OF SUMMER BATS IN THE NORTHEASTERN UNITED STATES","authors":"K. Francl, Tessa K. Canniff, Bland R. CRAIG, D. Sparks, V. Brack","doi":"10.5325/jpennacadscie.86.1.0041","DOIUrl":"https://doi.org/10.5325/jpennacadscie.86.1.0041","url":null,"abstract":"\u0000 While conducting mist net surveys for the federally endangered Indiana bat (Myotis sodalis) in West Virginia, New York, and Pennsylvania, we quantified wing damage on 422 bats of four species: big brown (Eptesicus fuscus, N = 190), eastern red (Lasiurus borealis, N = 82), little brown (M. lucifugus, N = 55), and northern myotis (M. septentrionalis, N = 95) bats. From 15 May – 15 August 2010, we photographed back-lit wings to reveal damage such as scars, holes, and blotching (non-uniform pigmentation). After quantifying the percent cover of these damage types using image-analysis software, we used generalized non-linear mixed models to determine if percent area of damage differed among scores associated with the categorical wing damage index (WDI) developed by Reichard and Kunz (2009). Although quantified damage did generally increase with WDI score across all species, statistical separation by WDI was only documented for the big brown bat (blotching, scars, blotching + scars combined) and northern myotis (blotching, blotching + scars). We suggest that studies like ours can provide quantitative species-specific datasets that can be examined in a more precise manner than a categorical index.","PeriodicalId":85037,"journal":{"name":"Journal of the Pennsylvania Academy of Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70852802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Bogacz, Collider Scenarios, Lemc Scenario, Rollin B. Johnson, Y. Alexahin
{"title":"PROGRAM SESSIONS","authors":"A. Bogacz, Collider Scenarios, Lemc Scenario, Rollin B. Johnson, Y. Alexahin","doi":"10.1364/josa.35.000797","DOIUrl":"https://doi.org/10.1364/josa.35.000797","url":null,"abstract":"","PeriodicalId":85037,"journal":{"name":"Journal of the Pennsylvania Academy of Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1364/josa.35.000797","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66720947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SCHEDULE OF ACTIVITIES AT A GLANCE","authors":"","doi":"10.5325/jpennacadscie.86.2.0080","DOIUrl":"https://doi.org/10.5325/jpennacadscie.86.2.0080","url":null,"abstract":"","PeriodicalId":85037,"journal":{"name":"Journal of the Pennsylvania Academy of Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70853435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE MARINE SCIENCE CONSORTIUM…HANDS-ON, FEET-WET EDUCATION","authors":"Thomas Tauer","doi":"10.5325/jpennacadscie.86.1.003","DOIUrl":"https://doi.org/10.5325/jpennacadscie.86.1.003","url":null,"abstract":"","PeriodicalId":85037,"journal":{"name":"Journal of the Pennsylvania Academy of Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70852614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE IMPACT OF WOOD TYPE, SEASON, AND REPELLENT USE ON NORTH AMERICAN PORCUPINE DAMAGE","authors":"Andie S. Graham, Tolley Roen Keely","doi":"10.5325/jpennacadscie.86.1.0036","DOIUrl":"https://doi.org/10.5325/jpennacadscie.86.1.0036","url":null,"abstract":"\u0000 We evaluated the impact of wood type, season, and repellent use on North American Porcupine damage to forty installed posts along Rails-to-Trails property in Brockway, Pennsylvania, USA. Treatments were (1) pressure-treated Pine posts with Ro-Pel (a liquid repellent), (2) pressure-treated Pine posts without Ro-Pel, (3) Black Locust posts with Ro-Pel, and (4) Black Locust posts without Ro-Pel. We assessed new damage to posts 1 – 4 times per month from June 2007 – January 2009. All damage was to Pine posts (x− = 12.9 cm2, SE = 3.70 cm2) and no damage was observed to Black Locust posts. Observed damage to untreated posts (x− = 9.93 cm2, SE = 2.10 cm2) was 3.3 times greater than damage to posts treated with Ro-Pel (x− = 2.99 cm2, SE = 2.86 cm2). Total monthly damage to Pine posts was greatest in August (x− = 2.31 cm2, SE = 1.51 cm2) and there was no observed damage in February, October, and November. When examining damage by season, damage to Pine posts was 54 times greater in summer (June – August; x− = 1.62 cm2, SE = 0.567 cm2) than in fall (September – November; x− = 0.030 cm2, SE = 0.030 cm2). We suspect the seasonality observed may be related to salt loss and salt-seeking behavior. We suggest that landowners and managers who anticipate Porcupine damage consider using alternatives to pressure-treated Pine for their wooden structures. In addition, repellent applications could be timed to correspond with the peak of damage to reduce cost.","PeriodicalId":85037,"journal":{"name":"Journal of the Pennsylvania Academy of Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70852746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"IONIC DEPENDENCY OF SKIN POTENTIAL, TRANSPORT CURRENT, AND THEIR RELATIONSHIP IN LEOPARD FROG (RANA SPP.) VENTRAL EPITHELIA","authors":"P. Nealen","doi":"10.5325/jpennacadscie.86.1.0005","DOIUrl":"https://doi.org/10.5325/jpennacadscie.86.1.0005","url":null,"abstract":"\u0000 Amphibian ventral epithelia can perform significant Na+ transport, as characterized in the now-classic two-membrane model first demonstrated by Ussing and colleagues. This transport is normally demonstrated by using short-circuit current (Isc) to negate transport-generated potentials across epithelia suspended in Ussing chambers. While this model and method have been supported through decades of experimentation, the exact relationship between the transport current and the epithelial potential generated is often ignored, as potentials typically are treated as a factor to be systematically eliminated. Here, leopard frog (Rana spp.) ventral epithelium were utilized in Ussing chambers for tests of specifically how the relationship between epithelial potentials and transport currents depends upon external medium ionic content. Stable skin potentials were recorded using 300 mOsm NaCl; potentials were then reduced to 0 mV via Isc in order to estimate the magnitude of the ionic transport current. A subset of the epithelia prepared was then tested in one or more alternative ionic solutions (300 mOsm KCl, NaHCO3, and CaCl2). While identifiable skin potentials were detected in all solutions tested, only in NaCl were the magnitudes of the epithelial potential and short-circuit current statistically significantly related. Detectable skin potentials in non-Na+-containing solutions indicates the existence of non-Na+ electrogenic activity in this tissue, whose presence explains the relatively poor ability of transport current magnitudes to explain the magnitudes of observed skin potential. Estimates of molecular transport rates for Na+ exceed 4 x 1014 molec sec-1 cm-1, demonstrating the enormous osmoregulatory challenge faced by fresh water amphibians in maintaining ionic homeostasis.","PeriodicalId":85037,"journal":{"name":"Journal of the Pennsylvania Academy of Science","volume":"278 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70852241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"LIGHT AND SCANNING ELECTRON MICROSCOPIC OBSERVATIONS OF THE LARVAL STAGES AND ADULT OF SPHAERIDIOTREMA GLOBULUS (TREMATODA: PSILOSTOMIDAE)","authors":"J. Huffman, P. Stevens, B. Fried","doi":"10.5325/jpennacadscie.86.1.0061","DOIUrl":"https://doi.org/10.5325/jpennacadscie.86.1.0061","url":null,"abstract":"\u0000 Sphaeridiotrema globulus (Rudolphi, 1814) is a digenetic trematode occurring in river and lentic aquatic systems in North America and has been identified as the cause of ulcerative hemorrhagic enteritis in waterfowl. The purpose of this study was to describe the stages of S. globulus using light (LM) and scanning electron microscopy (SEM). Elimia virginica snails infected with S. globulus were collected from Lake Musconetcong, NJ. Daughter rediae and cercariae were collected from the infected snails and LM and SEM microscopy were used to describe these stages with particular emphasis on the tegumentary surface. The tegument of S. globulus daughter rediae and cercariae is aspinose. The daughter rediae and cercariae showed simpler tegumentary structures than similar stages described in several species of echinostomids.","PeriodicalId":85037,"journal":{"name":"Journal of the Pennsylvania Academy of Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70852513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}