Asian Journal of Andrology最新文献_第6页

Patients with MMAF induced by novel biallelic CFAP43 mutations have good fertility outcomes after intracytoplasmic sperm injection. 由新的双等位基因CFAP43突变诱导的MMAF患者在细胞质内精子注射后具有良好的生育能力。

IF 3 2区医学

Asian Journal of Andrology Pub Date : 2023-09-01 Epub Date: 2023-03-21 DOI: 10.4103/aja2022118

Jing Ma, Shun-Hua Long, Hai-Bing Yu, Ye-Zhou Xiang, Xiang-Rong Tang, Jia-Xun Li, Wei-Wei Liu, Wei Han, Rong Jin, Guo-Ning Huang, Ting-Ting Lin

{"title":"Patients with MMAF induced by novel biallelic CFAP43 mutations have good fertility outcomes after intracytoplasmic sperm injection.","authors":"Jing Ma, Shun-Hua Long, Hai-Bing Yu, Ye-Zhou Xiang, Xiang-Rong Tang, Jia-Xun Li, Wei-Wei Liu, Wei Han, Rong Jin, Guo-Ning Huang, Ting-Ting Lin","doi":"10.4103/aja2022118","DOIUrl":"10.4103/aja2022118","url":null,"abstract":"As a specific type of asthenoteratozoospermia, multiple morphological abnormalities of the sperm flagella (MMAF) is characterized by composite abnormalities, including absent, short, coiled, angulation, and irregular-caliber flagella. Mutations in cilia- and flagella-associated protein 43 ( CFAP43 ) are one of the main causative factors of MMAF established to date. To identify whether there are other CFAP43 mutations related to MMAF and to determine the clinical outcomes of assisted reproductive technology for patients with MMAF harboring different mutations, we recruited and screened 30 MMAF-affected Chinese men using a 22-gene next-generation sequencing panel. After systematic analysis, seven mutations in CFAP43 , including five novel mutations and two previously reported mutations, were identified from four families and related to MMAF in an autosomal recessive pattern. Papanicolaou staining, immunofluorescence, and electronic microscopy further clarified the semen characteristics and abnormal sperm morphologies, including disorganized axonemal and peri-axonemal structures, of the CFAP43 -deficient men. The female partners of two patients were pregnant after undergoing assisted reproductive technology through intracytoplasmic sperm injection, and one of them successfully gave birth to a healthy boy. This study significantly expands the mutant spectrum of CFAP43 , and together with the available information regarding male infertility and MMAF, provides new information for the genetic diagnosis and counseling of MMAF in the future.","PeriodicalId":8483,"journal":{"name":"Asian Journal of Andrology","volume":" ","pages":"564-571"},"PeriodicalIF":3.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/83/6e/AJA-25-564.PMC10521949.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9913524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pudendal nerve lesions in young men with erectile dysfunction: imaging with 3T magnetic resonance neurography. 年轻男性勃起功能障碍患者的Pudendal神经损伤：3T磁共振神经成像。

IF 3 2区医学

Asian Journal of Andrology Pub Date : 2023-09-01 Epub Date: 2023-01-10 DOI: 10.4103/aja202293

Guo-Quan Huang, Tao Gong, Shan-Shan Wang, Qin-Hua Xia, Liang-Jie Lin, Guang-Bin Wang

引用次数: 0

Insufficiency of Mrpl40 disrupts testicular structure and semen parameters in a murine model. Mrpl40缺乏会破坏小鼠模型中的睾丸结构和精液参数。

IF 3 2区医学

Asian Journal of Andrology Pub Date : 2023-09-01 Epub Date: 2023-03-03 DOI: 10.4103/aja2022119

Ying Liu, Long-Long Fu, Hui-Zhong Xu, Yi-Ming Zheng, Wei-Xi Li, Guang-Hui Qian, Wen-Hong Lu, Hai-Tao Lv

{"title":"Insufficiency of Mrpl40 disrupts testicular structure and semen parameters in a murine model.","authors":"Ying Liu, Long-Long Fu, Hui-Zhong Xu, Yi-Ming Zheng, Wei-Xi Li, Guang-Hui Qian, Wen-Hong Lu, Hai-Tao Lv","doi":"10.4103/aja2022119","DOIUrl":"10.4103/aja2022119","url":null,"abstract":"Approximately 31% of patients with 22q11.2 deletion syndrome (22q11.2DS) have genitourinary system disorders and 6% of them have undescended testes. Haploinsufficiency of genes on chromosome 22q11.2 might contribute to the risk of 22q11.2DS. In this study, we used mice with single-allele deletion in mitochondrial ribosomal protein L40 ( Mrpl40 +/- ) as models to investigate the function of Mrpl40 in testes and spermatozoa development. The penetrance of cryptorchidism in Mrpl40 +/- mice was found to be higher than that in wild-type (WT) counterparts. Although the weight of testes was not significantly different between the WT and Mrpl40 +/- mice, the structure of seminiferous tubules and mitochondrial morphology was altered in the Mrpl40 +/- mice. Moreover, the concentration and motility of spermatozoa were significantly decreased in the Mrpl40 +/- mice. In addition, data-independent acquisition mass spectrometry indicated that the expression of genes associated with male infertility was altered in Mrpl40 +/- testes. Our study demonstrated the important role of Mrpl40 in testicular structure and spermatozoa motility and count. These findings suggest that Mrpl40 is potentially a novel therapeutic target for cryptorchidism and decreased motility and count of spermatozoa.","PeriodicalId":8483,"journal":{"name":"Asian Journal of Andrology","volume":" ","pages":"627-631"},"PeriodicalIF":3.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/68/04/AJA-25-627.PMC10521951.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9925084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commentary on "Clinical implications of endogenous testosterone density in prostate cancer progression in patients with favorable low and intermediate risk treated with radical prostatectomy". “内源性睾酮密度对接受根治性前列腺切除术的中低风险患者前列腺癌症进展的临床意义”的评论。

IF 3 2区医学

Asian Journal of Andrology Pub Date : 2023-09-01 Epub Date: 2023-01-10 DOI: 10.4103/aja2022114

Gian Maria Busetto, Ugo Giovanni Falagario, Giuseppe Carrieri

引用次数: 0

Modified onlay island flap versus tubularized incised plate urethroplasty for hypospadias reoperation. 改良岛状皮瓣与管状切开板尿道成形术治疗尿道下裂再次手术。

IF 3 2区医学

Asian Journal of Andrology Pub Date : 2023-09-01 Epub Date: 2023-03-14 DOI: 10.4103/aja20235

Xiao-Jiang Zhu, Yong-Ji Deng, Jun Wang, Yun-Fei Guo, Zheng Ge

{"title":"Modified onlay island flap versus tubularized incised plate urethroplasty for hypospadias reoperation.","authors":"Xiao-Jiang Zhu, Yong-Ji Deng, Jun Wang, Yun-Fei Guo, Zheng Ge","doi":"10.4103/aja20235","DOIUrl":"10.4103/aja20235","url":null,"abstract":"This study aimed to assess the outcomes of modified onlay island flap (MOIF) urethroplasty for hypospadias reoperation. A retrospective analysis was conducted using clinical data on children reoperated with MOIF or tubularized incised plate (TIP) urethroplasty for hypospadias in the Children's Hospital of Nanjing Medical University (Nanjing, China) from December 2016 to September 2020. A total of 90 children with hypospadias who failed primary repair and were reoperated on with MOIF (MOIF group, n = 46) or TIP urethroplasty (TIP group, n = 44) were recruited. All children were postoperatively followed up for at least 6 months. Six patients with postoperative urinary fistula, 2 patients with glans dehiscence, and 1 patient with urethral diverticulum were reported in the MOIF group, while 7 patients with postoperative urinary fistula, 2 patients with urethral stricture, and 1 patient with glans dehiscence were reported in the TIP group. Postoperative infection, flap necrosis and other complications were not recorded. A total of 37 (80.4%) patients in the MOIF group and 34 (77.3%) patients in the TIP group were successfully reoperated. Although the success rates of MOIF and TIP urethroplasty are comparable, pediatricians should master different types of repair techniques for individualized hypospadias reoperation. MOIF urethroplasty can be recommended after failure of the primary repair.","PeriodicalId":8483,"journal":{"name":"Asian Journal of Andrology","volume":" ","pages":"583-586"},"PeriodicalIF":3.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/64/54/AJA-25-583.PMC10521953.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9976897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a predictive model for increasing sperm retrieval success by microdissection testicular sperm extraction in patients with nonobstructive azoospermia. 非梗阻性无精子症患者通过显微切割睾丸精子提取提高精子回收成功率的预测模型的开发。

IF 3 2区医学

Asian Journal of Andrology Pub Date : 2023-09-01 Epub Date: 2023-02-07 DOI: 10.4103/aja2022111

Chen-Yao Deng, De-Feng Liu, Lian-Ming Zhao, Hao-Cheng Lin, Jia-Ming Mao, Zhe Zhang, Yu-Zhuo Yang, Hai-Tao Zhang, Kai Hong, Hui-Yu Xu, Hui Jiang

{"title":"Development of a predictive model for increasing sperm retrieval success by microdissection testicular sperm extraction in patients with nonobstructive azoospermia.","authors":"Chen-Yao Deng, De-Feng Liu, Lian-Ming Zhao, Hao-Cheng Lin, Jia-Ming Mao, Zhe Zhang, Yu-Zhuo Yang, Hai-Tao Zhang, Kai Hong, Hui-Yu Xu, Hui Jiang","doi":"10.4103/aja2022111","DOIUrl":"10.4103/aja2022111","url":null,"abstract":"Microdissection testicular sperm extraction (micro-TESE) is widely used to treat nonobstructive azoospermia. However, a good prediction model is required to anticipate a successful sperm retrieval rate before performing micro-TESE. This retrospective study analyzed the clinical records of 200 nonobstructive azoospermia patients between January 2021 and December 2021. The backward method was used to perform binary logistic regression analysis and identify factors that predicted a successful micro-TESE sperm retrieval. The prediction model was constructed using acquired regression coefficients, and its predictive performance was assessed using the receiver operating characteristic curve. In all, 67 patients (sperm retrieval rate: 33.5%) underwent successful micro-TESE. Follicle-stimulating hormone, anti-Müllerian hormone, and inhibin B levels varied significantly between patients who underwent successful and unsuccessful micro-TESE. Binary logistic regression analysis yielded the following six predictors: anti-Müllerian hormone (odds ratio [OR] = 0.902, 95% confidence interval [CI]: 0.821-0.990), inhibin B (OR = 1.012, 95% CI: 1.001-1.024), Klinefelter's syndrome (OR = 0.022, 95% CI: 0.002-0.243), Y chromosome microdeletion (OR = 0.050, 95% CI: 0.005-0.504), cryptorchidism with orchiopexy (OR = 0.085, 95% CI: 0.008-0.929), and idiopathic nonobstructive azoospermia (OR = 0.031, 95% CI: 0.003-0.277). The prediction model had an area under the curve of 0.720 (95% CI: 0.645-0.794), sensitivity of 65.7%, specificity of 72.2%, Youden index of 0.379, and cut-off value of 0.305 overall, indicating good predictive value and accuracy. This model can assist clinicians and nonobstructive azoospermia patients in decision-making and avoiding negative micro-TESE results.","PeriodicalId":8483,"journal":{"name":"Asian Journal of Andrology","volume":" ","pages":"598-603"},"PeriodicalIF":3.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f9/16/AJA-25-598.PMC10521962.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9976885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The emerging role of exosomes in the development of testicular. 外泌体在睾丸发育中的新作用。

IF 3 2区医学

Asian Journal of Andrology Pub Date : 2023-09-01 Epub Date: 2023-04-11 DOI: 10.4103/aja2022126

Chun-Yang Li, Song-Po Liu, Xiao-Fang Dai, Dong-Feng Lan, Tao Song, Xian-Yao Wang, Qing-Hong Kong, Jun Tan, Ji-Dong Zhang

{"title":"The emerging role of exosomes in the development of testicular.","authors":"Chun-Yang Li, Song-Po Liu, Xiao-Fang Dai, Dong-Feng Lan, Tao Song, Xian-Yao Wang, Qing-Hong Kong, Jun Tan, Ji-Dong Zhang","doi":"10.4103/aja2022126","DOIUrl":"10.4103/aja2022126","url":null,"abstract":"The mechanisms of testicular development in mammals are complex. Testis is an organ that produces sperm and secretes androgens. It is rich in exosomes and cytokines that mediate signal transduction between tubule germ cells and distal cells, promoting testicular development and spermatogenesis. Exosomes are nanoscale extracellular vesicles that transmit information between cells. By transmitting information, exosomes play an important role in male infertility diseases such as azoospermia, varicocele, and testicular torsion. However, due to the wide range of sources of exosomes, extraction methods are numerous and complex. Therefore, there are many difficulties in studying the mechanisms of exosomal effects on normal development and male infertility. Therefore, in this review, first, we introduce the formation of exosomes and methods for culturing testis and sperm. Then, we introduce the effects of exosomes on different stages of testicular development. Finally, we summarize the prospects and shortcomings of exosomes when used in clinical applications. We lay the theoretical foundation for the mechanism of the influence of exosomes on normal development and male infertility.","PeriodicalId":8483,"journal":{"name":"Asian Journal of Andrology","volume":" ","pages":"547-555"},"PeriodicalIF":3.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a8/41/AJA-25-547.PMC10521952.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9976927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of outcomes in three surgical techniques for proximal hypospadias: staged transverse preputial island flap urethroplasty versus single-stage repairs. 三种治疗近端尿道下裂的手术方法的疗效比较：一期包皮横岛状皮瓣尿道成形术与一期修复术。

IF 3 2区医学

Asian Journal of Andrology Pub Date : 2023-09-01 Epub Date: 2023-01-03 DOI: 10.4103/aja2022106

Yu-Si Wang, Hong-Cheng Song, Pei Liu, Yi-Wei Fang, Wei-Ping Zhang

{"title":"Comparison of outcomes in three surgical techniques for proximal hypospadias: staged transverse preputial island flap urethroplasty versus single-stage repairs.","authors":"Yu-Si Wang, Hong-Cheng Song, Pei Liu, Yi-Wei Fang, Wei-Ping Zhang","doi":"10.4103/aja2022106","DOIUrl":"10.4103/aja2022106","url":null,"abstract":"To evaluate and compare the outcomes and complications of three different surgical techniques for treating primary proximal hypospadias with ventral curvature (VC) ≥30°, we retrospectively reviewed the medical records of patients who underwent primary repair of proximal hypospadias with VC ≥30° after degloving at Beijing Children's Hospital Affiliated to Capital Medical University (Beijing, China) from January 2019 to January 2021. A total of 152 patients were divided into three groups: transverse preputial island flap (TPIF) combined with Duplay, modified Koyanagi, and staged TPIF, which were performed on 55, 16, and 81 patients, respectively. A total of 39 (25.7%) patients had complications. Complications rates were similar for the TPIF combined with the Duplay group (40.0%) and modified Koyanagi group (50.0%) but lower for the staged TPIF group (11.1%; P < 0.01). The incidence of urethrocutaneous fistulas was significantly higher in TPIF combined with Duplay group (21.8%) compared to staged TPIF group (4.9%; P = 0.01). In univariate analysis, the length of the urethral defect was the single factor that could predict complications; the cutoff was 4.55 cm. More patients in the long urethral defect group than in the short one had complications (34.1% vs 15.7%, P = 0.01). These results indicate that staged TPIF produced a better outcome, whereas more patients in the TPIF combined with Duplay group presented with two or more complications.","PeriodicalId":8483,"journal":{"name":"Asian Journal of Andrology","volume":" ","pages":"616-620"},"PeriodicalIF":3.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b7/ca/AJA-25-616.PMC10521944.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9922501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A decade of WHO 2010: total sperm number temporal trend and role of lifestyle factors. 世界卫生组织2010年十年：精子总数的时间趋势和生活方式因素的作用。

IF 3 2区医学

Asian Journal of Andrology Pub Date : 2023-09-01 Epub Date: 2023-01-17 DOI: 10.4103/aja202297

Francesco Cargnelutti, Francesco Pallotti, Tania Carlini, Fabiana Faja, Anna Rita Vestri, Danilo Alunni Fegatelli, Andrea Lenzi, Francesco Lombardo, Donatella Paoli

{"title":"A decade of WHO 2010: total sperm number temporal trend and role of lifestyle factors.","authors":"Francesco Cargnelutti, Francesco Pallotti, Tania Carlini, Fabiana Faja, Anna Rita Vestri, Danilo Alunni Fegatelli, Andrea Lenzi, Francesco Lombardo, Donatella Paoli","doi":"10.4103/aja202297","DOIUrl":"10.4103/aja202297","url":null,"abstract":"After a huge decline in sperm concentration between 1938 and 1991 was reported, many researchers investigated the possibility of a worsening of human sperm quality. Despite massive efforts, published evidence is still controversial. Similarly, the role of lifestyle factors on semen parameters is debated. We conducted a monocentric Italian study to evaluate the total sperm number trend over the last 10 years (from 2010 to 2019). Additionally, we evaluated the association between lifestyle factors and total sperm number in order to identify possible damaging factors. We performed a retrospective study analyzing subjects aged 18-55 years who had their semen analyzed between 2010 and 2019. A total of 3329 subjects were included: 1655 subjects referred to our department (Department of Experimental Medicine, Sapienza University of Rome, Roma, Italy) for idiopathic infertility and 1674 subjects referred for preconceptional or andrological screening with no confirmed andrological diseases. Semen samples were examined according to World Health Organization (WHO) 2010 criteria by two seminologists with the same training and the same equipment. For statistical evaluations, only total sperm number (×10 6 per ejaculate) was taken into consideration. We detected no significant changes in mean total sperm number during the last decade, in either the entire population or the two subgroups (infertile group and control group). In a multivariate analysis total sperm number was significantly associated with the history of infertility, body mass index (BMI) and cigarette smoking. Our results suggest that infertile men are \"vulnerable\" subjects, particularly susceptible to several negative factors, many of which still remain unknown. Our study highlights the need for studies addressing men's lifestyle in order to find and reduce deleterious agents.","PeriodicalId":8483,"journal":{"name":"Asian Journal of Andrology","volume":" ","pages":"572-577"},"PeriodicalIF":3.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c0/7c/AJA-25-572.PMC10521956.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10279786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testicular exosomes disturb the immunosuppressive phenotype of testicular macrophages mediated by miR-155-5p in uropathogenic Escherichia coli-induced orchitis. 在尿路致病性大肠杆菌诱导的睾丸炎中，睾丸外泌体干扰miR-155-5p介导的睾丸巨噬细胞的免疫抑制表型。

IF 2.9 2区医学

Asian Journal of Andrology Pub Date : 2023-05-01 DOI: 10.4103/aja202243

Jia Xu, Chao He, Yi-Wei Fang, Zhi-Yong Hu, Mei-Lin Peng, Yuan-Yao Chen, Yu-Fang Su, Chun-Yan Liu, Hui-Ping Zhang, Kai Zhao

{"title":"Testicular exosomes disturb the immunosuppressive phenotype of testicular macrophages mediated by miR-155-5p in uropathogenic Escherichia coli-induced orchitis.","authors":"Jia Xu, Chao He, Yi-Wei Fang, Zhi-Yong Hu, Mei-Lin Peng, Yuan-Yao Chen, Yu-Fang Su, Chun-Yan Liu, Hui-Ping Zhang, Kai Zhao","doi":"10.4103/aja202243","DOIUrl":"https://doi.org/10.4103/aja202243","url":null,"abstract":"Male reproductive infections are known to shape the immunological homeostasis of the testes, leading to male infertility. However, the specific pathogenesis of these changes remains poorly understood. Exosomes released in the inflammatory microenvironment are important in communication between the local microenvironment and recipient cells. Here, we aim to identify the immunomodulatory properties of inflammatory testes-derived exosomes (IT-exos) and explore their underlying mechanisms in orchitis. IT-exos were isolated using a uropathogenic Escherichia coli (UPEC)-induced orchitis model and confirmed that IT-exos promoted proinflammatory M1 activation with increasing expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in vitro. We further used small RNA sequencing to identify the differential miRNA profiles in exosomes and primary testicular macrophages (TMs) from normal and UPEC-infected testes, respectively, and identified that miR-155-5p was highly enriched in IT-exos and TMs from inflammatory testes. Further study of bone marrow derived macrophages (BMDMs) transfected with miR-155-5p mimic showed that macrophages polarized to proinflammatory phenotype. In addition, the mice that were administrated IT-exos showed remarkable activation of TM1-like macrophages; however, IT-exos with silencing miR-155-5p showed a decrease in proinflammatory responses. Overall, we demonstrate that miR-155-5p delivered by IT-exos plays an important role in the activation of TM1 in UPEC-induced orchitis. Our study provides a new perspective on the immunological mechanisms underlying inflammation-related male infertility.","PeriodicalId":8483,"journal":{"name":"Asian Journal of Andrology","volume":"25 3","pages":"389-397"},"PeriodicalIF":2.9,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9a/6a/AJA-25-389.PMC10226493.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9541138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2