{"title":"Antibiotics and Antifungal Resistance Patterns of Microbial Isolates from Dish Washing Sponges in the University of Port-Harcourt, Nigeria","authors":"Akomah-Abadaike, O. N., Chumu, E. A.","doi":"10.9734/ajob/2023/v19i1358","DOIUrl":"https://doi.org/10.9734/ajob/2023/v19i1358","url":null,"abstract":"This study examined the presence or contamination of bacteria in dishwashing sponges as well as the impact of various disinfectants on sponges. The total number of pathogenic organisms present in 120 sponges was determined using the nutritional agar (NA), MacConkey agar (MAC), Mannitol-Salt agar (MSA), Eosin-Methylene Blue agar (EMB), and Salmonella-Shigella agar (SSA) techniques. The efficacy of various disinfectants was evaluated using bleach, sanitizer, liquid soap, and boiled water for 30 minutes, while the remaining one served as a control sample. The result showed that household sponges had the lowest bacteria load across the five media with a mean bacteria count of 6.98 log CFU/g, followed by restaurant sponges with a mean count of 7.31 log CFU/g, and the highest bacteria load of 7.43 log CFU/g was obtained from hostel sponges. E. coli (40%), Klebsiella sp. (20%), Shigella sp. (15%), Staphylococcus sp. (20%), and Salmonella sp. (5) were the bacteria isolated and identified, whereas Aspergillus niger (65.6%) Penicillium oxalicum (9.4%), and Candida albicans (25%) were the fungi responsible for the contamination. Tarivid 25%, Reflacine 50%, Ciproflox 0%, Augmentin 50%, Gentamycin 0%, Streptomycin 0%, Ceporex 50%, Nalidixic acid 75%, Septrin 25%, and Amplicin 75% are the antibiotic resistant strains that have been identified. Griseofluvin eliminates both fungi at all concentrations that have been tested. Dishwashing sponges can be extremely contaminated, especially those used in the hostels on the University of Port Harcourt's Abuja campus. However, by applying basic and routine disinfection processes, the microbial contamination can be greatly reduced.","PeriodicalId":8477,"journal":{"name":"Asian Journal of Cell Biology","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76419865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Growth, Yield Attributes and Metabolite Components of Thickhead (Ebolo) (Crassocephalum crepidioides Benth.) as Affected by Soil Amendment","authors":"W. A. Oloriegbe, S. Agele","doi":"10.9734/ajob/2023/v19i2359","DOIUrl":"https://doi.org/10.9734/ajob/2023/v19i2359","url":null,"abstract":"A screenhouse experiment was conducted to determine the growth, yield attributes and bioactive components of thickhead (ebolo) as affected by soil amendments. Treatments were soil type (fertile soil and degraded land respectively amended with NPK @300kg/ha (F1), NPK@150kg/ha (F2), Poultry Manure@10t/ha (F3), Poultry Manure @5t/ha (F4), NPK @ 150kg/ha + Poultry Manure @5000t/ha, (F5), NPK @ 75kg/ha + Poultry Manure @ 2500t/ha (F6) and unamended control. Data were collected on soil properties, growth and biomass yields of thickhead and chemical, proximate and bioactive components of leaves. Results showed that soil type and amendment significantly affected growth, yield and bioactive components of thickhead. Growth of thickhead improved significantly for soil collected under fallow vegetation (S1) while application of NPK and poultry manure combination at 0.375g + 12.5g per plant significantly enhanced most of the measured variables of thickhead. Fertilizer amendment of soils enhanced leaf sodium, potassium, calcium and magnesium contents, poultry manure amendment improved moisture content and crude protein, while NPK increased significantly leaf ash and fat contents. Post-cropping chemical analysis of treated soils showed increases in pH, organic carbon, nitrogen, organic matter, available P, K, Na, Ca, and Mg of fertilizer-amended soils compare with the unamended. Thickhead (ebolo) can be grown both on fertile and degraded soils, soil amendment using NPK-poultry manure combinations enhanced the growth, yield and nutrition of thickhead and is recommended for its production.","PeriodicalId":8477,"journal":{"name":"Asian Journal of Cell Biology","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78945143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Triple-Action Antifungal Topicals, Microbiologist's Alarm","authors":"I. E. Kasamba","doi":"10.9734/ajob/2023/v19i1357","DOIUrl":"https://doi.org/10.9734/ajob/2023/v19i1357","url":null,"abstract":"Today, we are witnessing the development and marketing of triple action antifungals for the treatment of superficial mycoses. It is a mixture of antifungals, antibiotics and anti-inflammatory. The problem of this research was to verify the effects of this mixture as to its effectiveness on superficial mycoses. Thus, we set ourselves the following objectives: to identify the antifungals in the pharmacies of the cities of Likasi, Lubumbashi and Kolwezi, to determine their composition and to discuss this composition with the existing literature. \u0000Through a cross-sectional study, we identified thirty-four different antifungals in 588 pharmacies, of which 16 or 47.05% are triple action and made up of Azoles as antifungal, the antibiotic gentamicin, and corticosteroids as anti-inflammatory, alongside polyene, Echinocandins and flucytosine. It is the combination of antifungals with conventional non-antifungal agents reoriented for their action on the growth of fungi. They consist of antibacterial drugs and steroidal anti-inflammatories. This reorientation was supposed to have excellent antifungal activity and could prevent resistance. However, the presence of the antibiotic will reduce the composition of the colonizing microbiota and promote fungal growth and enhance fungal pathogenicity indirectly and the corticosteroid component may interfere with the therapeutic actions of the antifungal agent and may accelerate fungal growth, due to a decrease in the host's local immunological reaction, so that the underlying infection may persist, and the dermatophytes may even acquire the ability to invade the deeper tissues. So, in support, it would be interesting to favor antifungals without combinations than those combining antibiotics and anti-inflammatory which has an extremely high rate of recurrence.","PeriodicalId":8477,"journal":{"name":"Asian Journal of Cell Biology","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81357080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yusuf Rukayat Titilayo, Lawal Wasiu Sesan, Oyagbola Elizabeth Oyetayo, Ahmed Tawakalitu, Dagba Idayat, Atanda Afusa Omolabake, Hassan Qudrat Omolara, A. Alaya, T. M. Olorundare
{"title":"Fungi Associated with the Crop of a Local Fowl: A Public Health Problem","authors":"Yusuf Rukayat Titilayo, Lawal Wasiu Sesan, Oyagbola Elizabeth Oyetayo, Ahmed Tawakalitu, Dagba Idayat, Atanda Afusa Omolabake, Hassan Qudrat Omolara, A. Alaya, T. M. Olorundare","doi":"10.9734/ajob/2023/v19i1356","DOIUrl":"https://doi.org/10.9734/ajob/2023/v19i1356","url":null,"abstract":"This study was aimed at Isolating fungi associated with crop of a local fowl. But series of Research have been conducted on fungal contaminants associated with Broiler Liter , But we Have less reported on fungal contaminants of poultry . The goals of this study were to (1) Describe the nature and Features of litter fungal contaminants and (2) report the frequency of fungi species present.10 new and 20 old samples were collected from 10 farms.In addition, Air samples were also collected, and after various procedures, 14 different fungal genera were detected in new litter. With respect to old litter, 18 species of fungi were detected, with Fusarium the most frequently isolated (41.2%), followed by A. section Flavi. (27.3%), Oudeemansiela sp (9.9%), and Yeast (16.9%).A significant positive correlation was found between litter fungal Biomass and total fungal contamination. Different Morphology and quantification of species have important implications in the evaluation of potential adverse health risks to exposed Farm workers and animals. Indescriminate spreading of poultry litter in fields is a potential environmental health concern, since (Rhizopus spp and mucor spp) were isolated.","PeriodicalId":8477,"journal":{"name":"Asian Journal of Cell Biology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88843870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chitosan Application in Aquatic Feed and Its Impact on Fish and Shrimp Productivity","authors":"Y. Andriani, R. Pratama, I. Hanidah","doi":"10.9734/ajob/2023/v19i1355","DOIUrl":"https://doi.org/10.9734/ajob/2023/v19i1355","url":null,"abstract":"The culture of crustaceans, such as shrimp and crabs, in Indonesia has resulted in a significant increase in waste production, particularly in the form of shells and heads. This waste contains valuable components like chitin, chitosan, and carotenoids, which can be extracted and utilized in aquaculture activities. Some research results show that the use of chitosan, derived from chitin, in fish feed, is a functional polysaccharide with various biological activities, including antimicrobial, anti-inflammatory, and immune-stimulating effects. It also acts as a growth promoter in shrimp and improves the performance of farmed fish. Additionally, the inclusion of chitin and chitosan in fish feed formulations enhances digestibility, growth, and nutrient absorption. This paper provides a comprehensive review of the potential, nutritional value, and benefits of processed crustacean waste products, focusing on chitosan, and their impact on the productivity of fish in aquaculture.","PeriodicalId":8477,"journal":{"name":"Asian Journal of Cell Biology","volume":"1884 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86531060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physicochemical and Polycyclic Aromatic Hydrocarbons (PAHs) Analysis in Soil and Sediment from Vicinity of Ikot Akpaden, Akwa Ibom State, Nigeria","authors":"U. U. Ubong, E. Ikpe, I. Ekwere, Emaime J. Uwanta","doi":"10.9734/ajob/2023/v19i1354","DOIUrl":"https://doi.org/10.9734/ajob/2023/v19i1354","url":null,"abstract":"The study was undertaken to determine the levels of 16 priority PAHs in soil and sediments from some vicinities of Ikot Akpaden, Mkpat Enin. The soils were prepared using standard procedure in the laboratory and analyzed for 16 US EPA priority PAHs using Gas Chromatography-Mass Spectrophotometer (GC-MS). The soil samples were obtained from two sampling sites at Ikot Akpaden (Site A and C). Site C showed minimal PAHs concentration while site A recorded PAHs levels ranging from 0.01 to 4.25 mg/kg. According to European commission, the PAHs concentrations in soil samples of this study are moderate, but deleterious over time. The soils were negatively affected more with low molecular weight PAHs than High Molecular Weight PAHs.This study revealed that the sampling sites had low carcinogenic burden, hence would exhibit moderate adverse health effects to plants and animals. It was concluded that automobile repair activities in site A contributed to the soil contamination with PAHs; hence it should be properly sited.","PeriodicalId":8477,"journal":{"name":"Asian Journal of Cell Biology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82172289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jeferson Santana Quadros, Mateus Cardoso Barros, Rafael da Silva Paiva, Magnun Antonio Penariol da Silva, R. Nunes
{"title":"Determination of Bacterial Isolates from Cocoa Almonds during Fermentation in “saf’s” Agroforestry System in the Amazon","authors":"Jeferson Santana Quadros, Mateus Cardoso Barros, Rafael da Silva Paiva, Magnun Antonio Penariol da Silva, R. Nunes","doi":"10.9734/ajob/2023/v19i1353","DOIUrl":"https://doi.org/10.9734/ajob/2023/v19i1353","url":null,"abstract":"Agroforestry systems (SAFs) are an alternative for sustainable development as they enable the recovery of degraded areas and reduce deforestation, contributing to breaking the cycle of traditional family farming, so common in the Amazon region. The significant appearance of endophytic microorganisms, such as bacteria in cocoa almonds, can benefit its production commonly with fermentative bacteria. The aim of the study was to characterize the microbiota of cocoa beans during the fermentation process. The isolation of bacteria was performed from the collected samples; one of the applied procedures was the scraping of the dried and fermented cocoa almonds. Afterward, aliquots were subcultured in a Petri dish with a culture medium containing Blood agar and MacConkey agar to verify bacteria. Cultures were analyzed by counting colony-forming units (CFU/mL). Molecular analyses and sequencing were utilized to describe the microbial diversity. DNA sequencing and phylogenetic analyzes were performed to emphasize microbial morphology characterization. Gram-negative bacilli (Enterobacter spp. and Citrobacter spp.) and Gram-positive bacilli (Bacillus spp.) were found in cocoa beans after 72 h of fermentation. This work contributed to the characterization of endophytic bacteria in cocoa seeds, enabling in-depth studies of in vitro verification of the potential for biocontrol of these endophytic bacteria in cocoa cultivation.","PeriodicalId":8477,"journal":{"name":"Asian Journal of Cell Biology","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81738347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. S. Andini, A. Juniawan, Fahrul Irawan, ST. Rafi’ah, Devi Amelia
{"title":"Antibacterial Activity of Fresh Palm Fruit (Veitichia merillii) against Propionibacterium acnes Bacteria","authors":"A. S. Andini, A. Juniawan, Fahrul Irawan, ST. Rafi’ah, Devi Amelia","doi":"10.9734/ajob/2023/v19i1352","DOIUrl":"https://doi.org/10.9734/ajob/2023/v19i1352","url":null,"abstract":"Antibiotics or antimicrobials are a group of compounds, both natural, semi-synthetic and synthetic, which have the property of killing or inhibiting the growth of bacteria. The infection studied in this research is a skin infection caused by the bacterium Propionibacterium acnes. Several studies have examined the bioactivity potential of palm plant parts such as the toxicity of palm fruit seeds to Artemia salina larvae. but there is no data regarding the antimicrobial potential of the palm fruit itself. The novelty of this study lies in the maturity phase of the simplicia or fruit used, namely ripe, half-riped and unripe palm fruit and the type of pathogenic bacteria that was inhibited, namely Propionibacterium acnes which was carried out in vitro. The purpose of this study was to determine whether palm fruit has antimicrobial activity against Propionibacterium acnes and the ripening phase of the fruit has the highest antimicrobial activity. The research method used is laboratory experimental research. Antibacterial activity test of palm fruit against bacteria was carried out in vitro using the disc method with an activity indicator in the form of the diameter of the inhibition zone. Fresh palm fruit (Veitichia merillii) juice has antibacterial activity against the acne-causing bacteria Propionibacterium acnes, but has a different level of inhibition when viewed from the level of maturity. The highest antibacterial activity based on research results was observed at the ripeness level (yellow/orange color) which was indicated by the width of the inhibition zone of 23.9 mm.","PeriodicalId":8477,"journal":{"name":"Asian Journal of Cell Biology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88672688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Climbers of Rourkela Forest Division, Odisha, India: Food, Medicinal, Ecological and Economical Aspects","authors":"Jashabanta Sethi, Sugimani Marndi, Sanjeet Kumar","doi":"10.9734/ajob/2023/v18i4351","DOIUrl":"https://doi.org/10.9734/ajob/2023/v18i4351","url":null,"abstract":"Sunlight is a scarce resource for plants growing on forest floors, yet climbers could scale trees and bushes to reach the light. Climbers are vital because they help to lower temperature and keep the forest cooler on a hot season. They provide food, shelter and many more to the wildlife and local communities. Still, they are not much explored. Therefore, a survey was carried out in Rourkela Forest Division took place during March – April 2023, and enumerated climbers along with uses. Results revealed that about 53 species of climber belonging to 20 families are available in the study areas. They are used by local communities as food, medicine, source of livelihood and have ecological values. Therefore, authors summarised the diversity of climber and documented it in a scientific manner for future restoration and making plant for value addition in study areas.","PeriodicalId":8477,"journal":{"name":"Asian Journal of Cell Biology","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81170820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yoslianto, Y. Kaber, Eliezer V. Sirami, Selvi Tebaiy
{"title":"Mangrove Forest of Roswar Island, in the National Park of Cenderawasih Bay, the Province of West Papua","authors":"Yoslianto, Y. Kaber, Eliezer V. Sirami, Selvi Tebaiy","doi":"10.9734/ajob/2023/v18i4350","DOIUrl":"https://doi.org/10.9734/ajob/2023/v18i4350","url":null,"abstract":"Research on mangrove vegetation on Rowsar Island, in the area of the National Park of Cenderawasih Bay, This research was conducted from March to April 20223. West Papua Province conducted to identify and understand the condition of the mangrove forest. This research use quantitative methods by inventory with path plots. Based on the observations of mangrove vegetation on Roswar Island, 10 types of true mangroves identified, i.e Rhizopora mucronata, Rhizopora apiculata, Sonneratia alba, Xylocarpus granatum, Sonneratia caseolaris, Bruguiera gymnorrhiza, Avicennia marina, Avicennia rumphiana, Aegialitis annulata, dan Aegiceras floridum. The general mangroves found at every station are Rhizopora apiculata dan Rhizopora mucronata. Rhizopora apiculata grows in muddy, soft, deep, and waterlogged soil. Rhizopora apiculata does not like growing on harder substrates mixed with sand. The dominance rate can reach 90% of the vegetation growing in a location. Rhizopora mucronata grows in the same area as Rhizopora apiculata but more tolerant to hard substrates and sand. Mangrove forests on Roswar Island are still in good condition and extensive, the diversity of the mangrove plant species is relatively high and its natural regeneration is going well. The management of the mangrove forest areas requires coordination, integration, synchronization, and synergy across sectors, between many agencies and institutions.","PeriodicalId":8477,"journal":{"name":"Asian Journal of Cell Biology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73952015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}