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Prochymosin and pepsinogen potential activity and electrophoretic study of proteolytic fractions in the gastric and duodenal mucosal extracts from the first four day old lambs. 4日龄羔羊胃和十二指肠黏膜提取物蛋白水解组分的原凝乳酶和胃蛋白酶原电位活性及电泳研究。
Archivum veterinarium Polonicum Pub Date : 1994-01-01
M Wiśliński, W Cybulski
{"title":"Prochymosin and pepsinogen potential activity and electrophoretic study of proteolytic fractions in the gastric and duodenal mucosal extracts from the first four day old lambs.","authors":"M Wiśliński,&nbsp;W Cybulski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Potential proteolytic activity of prochymosin (PCh) and pepsinogen (PG) as well as relative activity of the proteolytic fractions in the mucosal extracts of the whole stomach, pyloric region of the stomach and duodenum were investigated. The main activity in the lambs stomach after birth demonstrated PCh however a small amount of PGA activity was also present. The highest activity of PCh from the whole stomach was observed in day 2 and 3, PG activity from the whole and pyloric region of the stomach increased in the first day of life and later was not significant by changed. Mucosal extracts of the whole stomach contained 3 to 4 of pepsinogen fractions of the fast migration (Pg1, Pg2, Pg3 and Pg4) in which Pg3 was of the highest activity in the majority of lambs. Pyloric region revealed 3 fractions: Pg2, of a small and Pg3 and Pg4 of high and equal activity. In duodenum Pg3 and Pg4 were of equal activity. The beginning of Pg2, Pg3 and Pg4 relative activity changes were observed in lambs stomach in respect to increase of Pg2 and decrease of Pg3 activity. The group of 3-4 fractions of slow electrophoretic migration contained probably progastricsin of small--and prochymosin of the highest activity within this group and a small non-pepsinogen fraction.</p>","PeriodicalId":8381,"journal":{"name":"Archivum veterinarium Polonicum","volume":"34 1-2","pages":"75-89"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19570356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Organism reaction to cryodestruction of the prostatic gland in dogs. 机体对犬前列腺冷冻破坏的反应。
Archivum veterinarium Polonicum Pub Date : 1994-01-01
M Galanty
{"title":"Organism reaction to cryodestruction of the prostatic gland in dogs.","authors":"M Galanty","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of the investigation was to determine the effect of cryodestruction of the healthy prostate on general condition of dogs. The research was performed on 18 dogs divided into two groups: control and experimental. In the dogs from the experimental group after the laparotomy procedure the prostate was frozen in the repeated cycle using the flat cryoapplicator. The observations were carried out for 6 months. General condition of dogs was controlled, blood was examined and urine analysed. The performed determinations included the activity of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, acid phosphatase and prostatic isoenzyme of acid phosphatase. It has been observed that the cryodestruction procedures of the prostatic gland are well tolerated by dogs. During 3 weeks after the surgery the number of leucocytes in blood increased. An increased drop of the blood cells was observed for 2 weeks after the procedure. Characteristic behaviour was observed in case of the prostatic isoenzyme of acid phosphatase. Immediately after the procedure the increased activity of that enzyme was observed and 24h later its decrease which remained for about 2 weeks. Then the isoenzyme activity increased again and that increase lasted for the period of 2 months.</p>","PeriodicalId":8381,"journal":{"name":"Archivum veterinarium Polonicum","volume":"34 1-2","pages":"55-62"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19570429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Concentration of iron, zinc, copper and lead in the brain of wild and domestic geese. 野鹅和家鹅脑中铁、锌、铜和铅的浓度。
Archivum veterinarium Polonicum Pub Date : 1994-01-01
E Kalisińska
{"title":"Concentration of iron, zinc, copper and lead in the brain of wild and domestic geese.","authors":"E Kalisińska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Concentration of Fe, Zn, Cu and Pb were determined in the brain tissue of wild (bean goose, Anser fabalis, n = 14, and white-fronted goose, A. albifrons, n = 3) and domestic (A. anser f. domestica, n = 13) geese. The results are referred to 1 g fresh weight (micrograms/g). Out of 17 wild geese, 4 individuals showed a very high Pb level. Statistically significant differences in concentrations of the metals studied were found between the white-fronted and domestic geese; for 3 metals (Fe, Zn, Cu), differences between the bean and white-fronted geese were statistically significant as well. The two bean goose types, tundra and taiga, were found to differ in terms of their brain Zn and Cu concentrations: the tundra type geese showed higher content of the metals than those in the taiga type.</p>","PeriodicalId":8381,"journal":{"name":"Archivum veterinarium Polonicum","volume":"34 1-2","pages":"107-16"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19570453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of iron on metabolism of vitamin C in chickens. 铁对鸡维生素C代谢的影响。
Archivum veterinarium Polonicum Pub Date : 1994-01-01
B Nagórna-Stasiak, J Lechowski, A Lazuga-Adamczyk
{"title":"The effect of iron on metabolism of vitamin C in chickens.","authors":"B Nagórna-Stasiak,&nbsp;J Lechowski,&nbsp;A Lazuga-Adamczyk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The objective of the paper was to show the effect of supplementation of iron on vitamin C synthesis in chickens. Iron administered per as caused than increase of vitamin C synthesis in chickens. The increase of vitamin C synthesis following iron supplementation is due to the alteration of L-gulono-gamma-oxidase activity in tissues; the enzyme taking part in last phase of vitamin C formation, and due to increase of D-glucuronic acid level, an intermediate product during vitamin C synthesis. High doses of iron cause the increase of vitamin C metabolism in chickens.</p>","PeriodicalId":8381,"journal":{"name":"Archivum veterinarium Polonicum","volume":"34 1-2","pages":"99-106"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19570358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of the low molecular weight nuclear polypeptide by isoelectrofocusing of nuclear proteins first separated by gel electrophoresis in SDS. SDS凝胶电泳先分离的核蛋白等电聚焦分析低分子量核多肽。
Archivum veterinarium Polonicum Pub Date : 1994-01-01
B Borowicz, J Domaniewski
{"title":"Analysis of the low molecular weight nuclear polypeptide by isoelectrofocusing of nuclear proteins first separated by gel electrophoresis in SDS.","authors":"B Borowicz,&nbsp;J Domaniewski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Some of the nuclear proteins, especially those tightly bound with nucleic acids, show a tendency to aggregate and some of them are also hydrophobic. Thus, SDS-containing buffers are useful for their analysis. The method of isoelectrofocusing of proteins separated by polyacrylamide gel electrophoresis in the presence of SDS was used for the analysis of the nuclear proteins. First, SDS-polyacrylamide gel electrophoresis of total chromatin proteins was conducted. The sample of the examined nuclear proteins after SDS-gel electrophoresis was isolated as a gel slice (thus corresponding to the particular molecular weight range) and used for isoelectrofocusing. This approach has been used for the analysis of about 19 kD nuclear polypeptide, tightly bound with DNA. The isoelectric point of this polypeptide has been estimated as 5.3.</p>","PeriodicalId":8381,"journal":{"name":"Archivum veterinarium Polonicum","volume":"34 3-4","pages":"241-7"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19857599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell-mediated and humoral immunity in bulls infected with IBR/IPV virus (BHV 1). 感染IBR/IPV病毒(BHV 1)的公牛细胞介导和体液免疫。
Archivum veterinarium Polonicum Pub Date : 1994-01-01
W Deptuła
{"title":"Cell-mediated and humoral immunity in bulls infected with IBR/IPV virus (BHV 1).","authors":"W Deptuła","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Time-related changes in specific cell-mediated immunity (CMI) and in humoral immunity were monitored in 20 bulls, aging 12 to 16 months, in four groups, each consisting of 5 animals. Group I was experimentally and group III-naturally infected with BHV 1 virus, groups II and IV serving as control animals. Tests for CMI included delayed type hypersensitivity (DTH), leukocyte migration inhibitory factor (LMIF) and granulocyte migration inhibitor factor (GMIF-LIF-buffy coat leukocyte migration inhibitory factor) in the presence of BHV I antigen while humoral immunity was tested by serum induced neutralization of the virus (SN) and by estimation of serum IgG, IgG1, IgG2, IgM and IgA levels. Immunologic, virologic and clinical studies were performed two days before infection and 17 timed within 91 days after the infection in bulls of group I and II and 7 times within 42 days after developing the disease in bulls of groups III and IV. The results showed that positive CMI reactions appeared 3 to 4 weeks earlier than positive antibody titers in SN test. The antibodies belonged most probably to IgG1 and IgG2 subclasses.</p>","PeriodicalId":8381,"journal":{"name":"Archivum veterinarium Polonicum","volume":"34 1-2","pages":"13-23"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19570422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cryodestruction technique in the prostatic gland in dogs. 犬前列腺冷冻破坏技术。
Archivum veterinarium Polonicum Pub Date : 1994-01-01
M Galanty, R Lechowski
{"title":"Cryodestruction technique in the prostatic gland in dogs.","authors":"M Galanty,&nbsp;R Lechowski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The investigation aimed at selecting the best parameters of cryodestruction of the healthy prostatic gland in dogs. Freezing was performed from the external side of the gland. The investigations were carried out on 2 healthy dogs in which the prostatic glands were frozen with two different methods and then, 4 weeks later, the degree of the gland size decrease was estimated. Both dogs were subjected to the unifocal cryodestruction of the lobes of the prostate. The comparison was made of the usefulness of the flat and spear-headed applicators in the freezing procedure performed in a single or repeated cycle. Also two methods of taking the temperature of the urethra to protect it against overfreezing were compared. It was observed that the greatest decrease of the gland (by 55%) was obtained while using flat applicator and the repeated freezing cycle. Flat applicator does not cause any significant bleeding after the gland thawing.</p>","PeriodicalId":8381,"journal":{"name":"Archivum veterinarium Polonicum","volume":"34 1-2","pages":"37-44"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19570426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological and hormonal changes after superovulation in cows treated with Neutra-PMSG. 中性pmsg对奶牛超排卵后形态学和激素的影响。
Archivum veterinarium Polonicum Pub Date : 1994-01-01
Z Boryczko, H Bostedt, Z Gajewski, M Witkowski, B Hoffmann
{"title":"Morphological and hormonal changes after superovulation in cows treated with Neutra-PMSG.","authors":"Z Boryczko,&nbsp;H Bostedt,&nbsp;Z Gajewski,&nbsp;M Witkowski,&nbsp;B Hoffmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Morphological changes in ovaries and hormonal changes as well as changes in Na, K, Ca, Mg, and P blood plasma levels were observed after superovulation induced by the administration of PMSG and the neutralization of this hormone with monoclonal antibodies Neutra-PMSG administered either 72 or 108 h later. The introduction of Neutra-PMSG 108 h after PMSG injection clearly decreases the number of surviving non-ovulated follicles with a diameter > 10 mm (1.7 +/- 0.8 vesicle in an individual cow on the average) in comparison to the group without Neutra-PMSG (19.4 +/- 9.5). The efficiency of ovulation in the group treated with Neutra-PMSG in the 108th h of the experiment (8.2 +/- 4.6 corpora lutea per cow on the average), did not differ statistically from the group treated with PMSG only (12.4 +/- 10.6). Early administration of Neutra-PMSG (72h), totally inhibits superovulation. Observations showed, that injection of Neutra-PMSG in the 108th h, caused a considerable decrease in the estradiol level, beginning with the 120th h of the experiment. Determination of the progesterone blood plasma level reflects the number of corpora lutea and can be helpful in evaluating the effects of superovulation. Superovulation did not effect the level of Na, K, Ca, Mg, and P in the blood plasma.</p>","PeriodicalId":8381,"journal":{"name":"Archivum veterinarium Polonicum","volume":"34 1-2","pages":"117-26"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19570454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An evaluation of the resistance to Microsporum canis on the basis of the guinea pig. 基于豚鼠对犬小孢子虫的抗性评价。
Archivum veterinarium Polonicum Pub Date : 1994-01-01
K Wawrzkiewicz, G Ziółkowska, J Wawrzkiewicz
{"title":"An evaluation of the resistance to Microsporum canis on the basis of the guinea pig.","authors":"K Wawrzkiewicz,&nbsp;G Ziółkowska,&nbsp;J Wawrzkiewicz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The purpose of the work was to assess the immune response of guinea pigs after the experimental infection with Microsporum canis, and after immunization with a specific live vaccine. The guinea pigs after the recovery from infection showed a delayed type of hypersensitivity and in addition, 30 per cent of the animals were characterized by the presence of immediate hypersensitivity reactions. All the animals were resistant to reinfection with M. canis and to some extent to Trichophyton verrucosum, however, no cross-immunity against T. mentagrophytes was found. The vaccinated animals were characterized mainly by delayed type of hypersensitivity and the resistance to approximately 1000 infectious doses of M. canis.</p>","PeriodicalId":8381,"journal":{"name":"Archivum veterinarium Polonicum","volume":"34 3-4","pages":"153-62"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19855329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the proteins of bovine and ovine parainfluenza-3 viruses. 牛和羊副流感-3病毒蛋白的比较。
Archivum veterinarium Polonicum Pub Date : 1994-01-01
J Kita, U B Balasuriya, B I Osburn
{"title":"Comparison of the proteins of bovine and ovine parainfluenza-3 viruses.","authors":"J Kita,&nbsp;U B Balasuriya,&nbsp;B I Osburn","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bovine and ovine parainfluenza-3 (PI-3) virus proteins were compared using polyclonal and monoclonal antibodies in western blot analysis. Eight proteins ranging in molecular weight (MW) from 14 kD to 190 kD of bovine PI-3 virus were demonstrated in this study. The 14 kD MW protein has not been previously described for bovine PI-3 virus. Western blot analysis of ovine PI-3 virus using homologous serum produced 7 bands ranging in MW from 12 kD to 190 kD described for the first time in this paper. Using rabbit polyclonal antibodies against bovine PI-3 virus a 160 kD MW protein was demonstrated in the bovine virus strain, but not in the ovine PI-3 virus strain and a protein band appeared in the ovine PI-3 virus strain at a MW of 23 kD but not in the bovine PI-3 virus strain. Using lamb polyclonal antibodies to ovine PI-3 virus against the homologous virus, 6 protein bands were visible with a MW range from 12 kD to 175 kD; for bovine PI-3 virus 5 protein bands were resolved. There was a difference observed in the mobility of the 120 kD MW protein for ovine PI-3 viral protein but not for the bovine PI-3 viral protein. Differences were observed for ovine PI-3 viral protein at the level of the 12 kD MW protein whereas the lowest detected band for the bovine PI-3 viral protein was 37 kD MW. The monoclonal antibodies prepared against bovine PI-3 virus produced 3 distinct protein bands. Two bands of MW 110 and 140 kD were identical for both viruses whereas the third band for bovine PI-3 virus was 37 kD MW and that for ovine PI-3 virus was 55 kD MW.</p>","PeriodicalId":8381,"journal":{"name":"Archivum veterinarium Polonicum","volume":"34 3-4","pages":"195-204"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19855333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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