H. C. Nguyen, Thi Tho Nguyen, Thi Nhung Lam, Thi Trang Le, Phuong Lan Le, Tien Hung Nguyen, Xuan Hoan Dinh
{"title":"Pathogenicity structure of the Xanthomonas oryzae pv. oryzae population causing rice bacterial blight in Vietnam","authors":"H. C. Nguyen, Thi Tho Nguyen, Thi Nhung Lam, Thi Trang Le, Phuong Lan Le, Tien Hung Nguyen, Xuan Hoan Dinh","doi":"10.1080/03235408.2023.2184230","DOIUrl":"https://doi.org/10.1080/03235408.2023.2184230","url":null,"abstract":"Abstract The rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) bacteria is rice’s most destructive disease. Due to the potential biomagnification caused by bactericides, deploying host resistance is the most effective method to protect the rice harvest. Studying the pathogenicity structure of the Xoo population helps propose the best breeding strategies to use the resistance gene pool. Among 12 resistance genes validated, xa5 and Xa21 maintained their efficacy against the Xoo population in Vietnam. Other genes, including Xa7 and xa13, remained effective in certain regions. The most destructive Xoo races distribute in the South, where intensive rice production was practiced. The same Xoo races found in different ecological regions illustrated the pathogen migration between adjacent areas. This manuscript proposed a designation system that converts the response of the differential set to each Xoo isolate into a binary, then into an octal system to name Xoo races appropriately.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"256 - 268"},"PeriodicalIF":1.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47654175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Abdelmaksoud, A. El-Bakry, E. Sammour, N. F. Abdel-Aziz
{"title":"Comparative toxicity of essential oils, their emulsifiable concentrates and nanoemulsion formulations against the bean aphid, Aphis fabae","authors":"N. Abdelmaksoud, A. El-Bakry, E. Sammour, N. F. Abdel-Aziz","doi":"10.1080/03235408.2023.2178065","DOIUrl":"https://doi.org/10.1080/03235408.2023.2178065","url":null,"abstract":"Abstract The present study aims to prepare emulsifiable concentrate (EC) and nanoemulsion formulations from the plant essential oils of Lavandula angustifolia (Mill.), Ambrosia maritima (L.), Commiphora myrrha (Nees) Engl., Juniperus virginiana (L.), Rheum palmatum (L.) and Tagetes minuta (L.). The efficiency of these essential oils along with their formulations were compared against the bean aphid, Aphis fabae (Scopoli). Results disclosed that all EC preparations passed the emulsion stability and foam formation tests. The particle droplet size of the nanoemulsions varied from 64.3 to 264.7 nm. The toxicity of EC formulations was slightly higher than the corresponding nanoemulsion formulations. Both EC and nanoemulsion of R. palmatum were the most efficient (LC50 = 1003.60 and 1105.34 mg L−1, respectively). EC preparations had no adverse effects on seed beans. The absence of organic solvents and the high aphicidal activity of EC formulations might make these preparations suitable for controlling A. fabae.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"187 - 208"},"PeriodicalIF":1.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47150166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Varietal resistance of Algerian bread wheat against Fusarium culmorum, the main agent of crown rot on wheat","authors":"Hamza Bouanaka, I. Bellil, D. Khelifi","doi":"10.1080/03235408.2023.2178064","DOIUrl":"https://doi.org/10.1080/03235408.2023.2178064","url":null,"abstract":"Abstract Fusarium crown rot (FCR) is one of the serious wheat diseases. FC11 is one strain among 34 isolates obtained in a previous study, and identified as Fusarium culmorum strain highly pathogenic. FC11 was assigned the accession number MW151664 by NCBI GenBank. Two resistance/sensitivity tests were conducted on 9 cultivars (cv) of bread wheat. The In vitro test in the oven with the parameters; percentage of germination inhibition (%GI), to determine the tolerance of seedlings germination, and the Area Under the Disease Progress Curve (AUDPC) to test the tolerance to the initial infection of germinated coleoptiles. In in-vivo test in the growth chamber, the disease severity (DS) parameter used to test the cultivars ability to tolerate FCR induced by F. culmorum. The results showed that all cultivars were susceptible to FC11 with a significant decrease in germination and coleoptile emergence rate with GI% and AUDPC values reached up to 68.35, and 68.98% for cv. Boumerzoug and Hidhab, respectively. The cv. Bordj Mehis and Ain Abid showed significant susceptibility to F. culmorum, where length of the vegetative system and fresh weight (RCL% and RCW%) were negatively affected up to 28.66 and 56.66%, respectively. In this work, the cv. Akhamokh showed an interesting level of FCR tolerance among other cultivars tested.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"175 - 186"},"PeriodicalIF":1.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48528541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultrastructural studies of the host (Senecio aegyptius L.) responses after the infection by the pathogenic rust fungus, Puccinia lagenophorae Cooke","authors":"Z. Baka","doi":"10.1080/03235408.2023.2184229","DOIUrl":"https://doi.org/10.1080/03235408.2023.2184229","url":null,"abstract":"Abstract This study investigated the impact of the rust fungus, Puccinia lagenophorae on its host, Senecio aegyptius using both light (LM) and transmission (TEM) electron microscopy. The chloroplast numbers decreased during the aecial and telial stages of the pathogen (53.7 and 56.6%, respectively). The nucleus volumes were increased during both stages (10.9 and 14.1%, respectively). Infected chloroplasts and nuclei showed changes in their shape and ultrastructure. Instead of the M-haustorium, a collar was created around the D-haustorium. A neck encasing developed in both types of necrotic haustoria. This encasement may serve as the host cell’s protection against toxins created after the death of haustoria. The severe changes in the chloroplasts and nuclei of this host plant after infection by this pathogen may give this pathogen the possibility of controlling this serious weed. Future research in the molecular mechanism may explore the biological control of weeds for local farm agroecology.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"234 - 255"},"PeriodicalIF":1.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47364343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Interactions between fungal endophytes and pathogens isolated from the invasive plant kudzu (Pueraria montana var. lobata)","authors":"M. Shahrtash, S. Brown","doi":"10.1080/03235408.2023.2178055","DOIUrl":"https://doi.org/10.1080/03235408.2023.2178055","url":null,"abstract":"Abstract In this study, we examined the in vitro interactions between fungal endophytes and pathogens isolated from the invasive plant kudzu (Pueraria montana var. lobata) and test if endophytes might facilitate pathogen growth. This represents a required initial step towards the development of candidate fungal inocula that can aid in the suppression of kudzu. While most tested endophyte-pathogen assays suggest antagonism and/or competitive exclusion, we identified several pathogen/endophyte combinations that suggest pathogen facilitation. Additional work is needed to test if these may have in planta effects on phytopathogenicity. The present study accentuates on the potential of fungal endophytes as an effective ecological approach to manage invasive plants via pathogens facilitation.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"87 - 107"},"PeriodicalIF":1.0,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45493542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nikkie Ratya Alma, S. Indarti, S. Hartono, A. Soffan
{"title":"Evaluation of nematode DNA extraction methods for species identification of root rice nematode (Hirschmanniella spp.)","authors":"Nikkie Ratya Alma, S. Indarti, S. Hartono, A. Soffan","doi":"10.1080/03235408.2023.2178057","DOIUrl":"https://doi.org/10.1080/03235408.2023.2178057","url":null,"abstract":"Abstract The species-level identification of rice root nematode (Hirschmanniella spp.) is an important effort in pest management. Meanwhile, the selection of DNA extraction methods is the first important step in the molecular identification. Thereby, this study aimed to obtain the DNA extraction method and optimal quantity in the identification of Hirschmanniella spp. to the species level. The DNA extraction methods used were Cetyltrimethylammonium bromide (CTAB) and Commercial Kit (Geneaid; Genomic DNA Mini Kit Tissue), with variations in the number of nematodes: 1, 5, 10, 15 and 20 nematodes. There were no significant differences on DNA concentration and purity between CTAB and commercial kit method, but the DNA extraction of Hirschmaniella spp. with the CTAB method reached the highest DNA concentration of 189.57 ng/µL. DNA purity level in the ratio (A260/A280) ranging from 1.80 to 1.99. The PCR test obtained the amplification of the extracted DNA at ∼766 bp.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"127 - 136"},"PeriodicalIF":1.0,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42620656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Synergistic effect of Pseudomonas azotoformans and Trichoderma gamsii in management of Fusarium crown rot of wheat","authors":"K. Makhlouf, K. Boungab, S. Mokrani","doi":"10.1080/03235408.2023.2178056","DOIUrl":"https://doi.org/10.1080/03235408.2023.2178056","url":null,"abstract":"Abstract Fusarium crown rot is the major soil-borne disease of wheat and occurs throughout cereal producing areas of the world including Algeria. This study evaluated 10 bacterial and fungal strains, originating from Algeria as potential biological control agents against three soil-borne phytopathogens responsible for Fusarium crown rot of wheat (Fusarium pseudograminearum ON687723, F. graminearum ON685926 and F. equisti MK361175). All benificial microbial strains tested inhibited the pathogens mycelial growth in vitro. Isolate P58 (Pseudomonas azotoformans NR_113600) was the most effective antagonist against all the test pathogens in direct confrontation assay, and exhibited average of 70% growth inhibition of Fusarium spp. Isolate T2 (Trichoderma gamsii MK361138) proved suppression efficacy in the range of 61.87 to 71.64% toward plant pathogens. Under greenhouse conditions, wheat seeds treated with isolate P58 and T2 separately reduced the severity of crown rot disease significantly and improved the growth parameters of wheat seedlings as compared to control. The combined use of Pseudomonas azotoformans and Trichoderma gamsii increased the effectiveness of the antagonists and promoted wheat growth. Thus, it can be concluded that these strains can be utilized as a biocontrol agent for management of soilborne cereal pathogens.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"108 - 126"},"PeriodicalIF":1.0,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43920907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tarekegn M. Mekuria, Beira-Hailu Meressa, Wakuma B. Hundesa
{"title":"Prevalence of major parasitic nematodes associated with tomatoes (Solanum lycopersicum L.) in two districts of Jimma, Ethiopia","authors":"Tarekegn M. Mekuria, Beira-Hailu Meressa, Wakuma B. Hundesa","doi":"10.1080/03235408.2023.2178062","DOIUrl":"https://doi.org/10.1080/03235408.2023.2178062","url":null,"abstract":"Abstract This study assessed the diversity of plant parasitic nematodes associated with tomatoes in two major tomato producing districts. A total of 40 composite soil and root samples were collected, and extracted nematodes were identified morphologically to a genus level, however, Meloidogyne arenaria was identified based on molecular methods. Accordingly, eight plant parasitic nematode genera were identified, including Meloidogyne, Pratylenchus, Helicotylenchus, Scutellonema, Rotylenchulus, Criconema, Paratylenchus, and Globodera. The highest prominence value was recorded for Meloidogyne and the lowest being for Paratylenchus. In terms of Meloidogyne infestation, the highest mean root knot incidence was in the Dedo district, specifically at the Babo peasant association, where Meloidogyne could be considered a major threat to tomato production. The study further indicated that Meloidogyne density was higher in soil with a pH between 6.4 and 7.4, and an altitude above 1800 m. This result implies the need for immediate attention and implementation of feasible management strategies to reduce the damage caused by Meloidogyne sp.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"158 - 174"},"PeriodicalIF":1.0,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41440798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mycelial and sexual compatibility in plant pathogen, Leucostoma cinctum","authors":"Somayeh. Bakhshi, K. Fotouhifar, M. JAVAN-NIKKHAH","doi":"10.1080/03235408.2023.2178058","DOIUrl":"https://doi.org/10.1080/03235408.2023.2178058","url":null,"abstract":"Abstract The canker disease caused by Leucostoma cinctum is one of the most destructive diseases in fruit trees in Iran. For investigation of mycelial/sexual compatibility in L. cinctum, 64 isolates from 10 different plants, and 11 provinces of Iran were used. Isolates were crossed in all possible combinations on PDA at 25 °C for mycelial compatibility, and 14 multi-merge MCGs were identified among the isolates, revealing the average genetic diversity. In vitro sexual reproduction of the fungus was assessed using 14 ascospore progeny of two different perithecia, and the isolates were crossed in all possible pairwise combinations on culture media amended by different sterilized twigs. In four crosses on oatmeal agar with 2.5% sucrose, and apricot twigs, fertile perithecia were formed after 5 months, confirming heterothallic nature of the L. cinctum. Also, this is the first report of the in vitro viable sexual reproduction of the L. cinctum in the world.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"137 - 157"},"PeriodicalIF":1.0,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43296884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marziye Karimzade, M. Safarnejad, S. Aminzadeh, Hashem Kazemzadeh‐Beneh, F. Hemmati, M. Shams-bakhsh
{"title":"Generation of specific antibody against recombinant major coat protein of Beet necrotic yellow vein virus and its application for detection of rhizomania disease","authors":"Marziye Karimzade, M. Safarnejad, S. Aminzadeh, Hashem Kazemzadeh‐Beneh, F. Hemmati, M. Shams-bakhsh","doi":"10.1080/03235408.2023.2170205","DOIUrl":"https://doi.org/10.1080/03235408.2023.2170205","url":null,"abstract":"Abstract Beet necrotic yellow vein virus (BNYVV) is one of the main contributors to economic losses in sugar beet production. The present study generated a polyclonal antibody that detects BNYVV. The conserved genomic region of all BNYVV isolates gene encoding the viral major coat protein (BNYVV-CP) was amplified, cloned, sequenced, and expressed in E. coli strain BL21 (DE3). The expressed protein was purified under native conditions by affinity chromatography. The purified BNYVV CP was used as a recombinant antigen to raise polyclonal antibodies in rabbits. Purified anti-CP-IgG detected the BNYVV-CP recombinant protein and BNYVV in infected sugar beet by indirect-ELISA, dot immunobinding assay, and western blot analysis blotting. The serological assays strongly proved the sensitivity and specificity of anti-BNYVV-CP-IgG against BNYVV. These results suggest that the generated anti-BNYVV-CP-IgG polyclonal antibodies can be used as reliable and quick means for the BNYVV virus detection under field conditions.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"10 - 28"},"PeriodicalIF":1.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47498290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}