Archives of Pharmaceutical Sciences Ain Shams University最新文献_第10页

Assessing the impact of wastewater irrigation on cobalt cadmium and lead contents of grains of Sorghum bicolor 废水灌溉对双色高粱籽粒钴、镉、铅含量的影响

Archives of Pharmaceutical Sciences Ain Shams University Pub Date : 2019-01-01 DOI: 10.21608/APS.2019.20255

Mohamed M. Taher, Mahfoudh M. S. Al-Hammadi

{"title":"Assessing the impact of wastewater irrigation on cobalt cadmium and lead contents of grains of Sorghum bicolor","authors":"Mohamed M. Taher, Mahfoudh M. S. Al-Hammadi","doi":"10.21608/APS.2019.20255","DOIUrl":"https://doi.org/10.21608/APS.2019.20255","url":null,"abstract":"The impact of waste irrigation on the concentrations of the metals Cd, Co and Ni contents in grains of Sorghum bicolor was investigated through analyzing (for three seasons 2008-2010s) four different-irrigation sorghum grains samples grown on four different-irrigation soil areas according to the experimental design. Two original soils: Soil, historically and experimentally, irrigated with Wastewater (SHEwastewater), and Soil, historically and experimentally, irrigated with wells water samples were analyzed in the 1st and 2nd seasons. According to the procedures used in the literature, Samples were collected, pretreated, preserved, digested according to the microwave assisted acids digestion procedures, and analyzed for metals by ICP-AES. Quality control was performed and %R(s) we have gotten were good for real samples analysis. Cd and Pb metal contents of the two types of the original soil samples were not significantly different. In addition, these metal contents of the two types of soil samples have not exceeded the upper EU standards. The average means (of 1st and 2nd seasons) levels of Cd and Pb of wastewater were higher than that of wells water. In addition, the average means of these metal ions of both wastewater and wells water was lower than Yemeni standard for irrigation water. Sorghum grains analysis results, for at least two of the three seasons, indicated that: wastewater irrigation resulted in an increase in Cd and Pb contents of grains of Sorghum bicolor. Moreover, there is no significant difference between the effect of wastewater and wells water irrigation on Co content of grains of Sorghum bicolor.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82719879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microwave-assisted extraction of the gallic acid biomarker from Acacia arabica bark followed by HPLC analysis 微波辅助提取阿拉比卡树皮中没食子酸生物标志物并进行HPLC分析

Archives of Pharmaceutical Sciences Ain Shams University Pub Date : 2019-01-01 DOI: 10.21608/APS.2019.20240

Hend Z. Yamani, L. Hussein, M. Ghany

{"title":"Microwave-assisted extraction of the gallic acid biomarker from Acacia arabica bark followed by HPLC analysis","authors":"Hend Z. Yamani, L. Hussein, M. Ghany","doi":"10.21608/APS.2019.20240","DOIUrl":"https://doi.org/10.21608/APS.2019.20240","url":null,"abstract":"An efficient and fast microwave-assisted extraction (MAE) technique was developed for extracting gallic acid as an indicative biomarker for the quality control of Acacia arabica bark. The MAE technique was optimized and compared with other conventional extraction techniques. The optimal conditions of MAE were 20% methanol as solvent, solid/liquid ratio 1:40 (g/mL), irradiation power 20% and two extraction cycles, 5 min each. The proposed extraction technique produced a maximum yield of 10.59 (mg/g) gallic acid in 10 min, which was 1.03 and 1.15 times more efficient than 6 h of heat reflux and 24 h of maceration extraction, respectively. This high yield, along with saving of time, energy, and solvent would position MAE as a valuable and cost-effective technology suitable for today's highly competitive industries, with growing demand for increased productivity, improved efficiency, and reduced cycle time. Moreover, a new high-performance liquid chromatography method was developed and validated for the determination of gallic acid in Acacia arabica bark extract. The method was found to be rapid, sensitive, accurate, precise, and robust. The method showed good linearity over concentration range 1-100 (µg/mL) with LOD 16.08 (ng/mL) and LOQ 48.73 (ng/mL). The average recovery obtained using standard addition technique was 100.36% with a low value of RSD% (1.19%) indicating the accuracy of the proposed method for determination of gallic acid in Acacia arabica bark extract.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87696932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of esomeprazole and pantoprazole on renal function in stable kidney transplantation recipients: A randomized clinical trial. 埃索美拉唑和泮托拉唑对稳定肾移植受者肾功能的影响:一项随机临床试验。

Archives of Pharmaceutical Sciences Ain Shams University Pub Date : 2019-01-01 DOI: 10.21608/aps.2019.10411.1003

Doaa El-Bohy, M. Sharkawy, Soheir Abo-Elazm, S. Shahin, Waleed Bchari, Azza Manc, M. E. Hamamsy

{"title":"Effects of esomeprazole and pantoprazole on renal function in stable kidney transplantation recipients: A randomized clinical trial.","authors":"Doaa El-Bohy, M. Sharkawy, Soheir Abo-Elazm, S. Shahin, Waleed Bchari, Azza Manc, M. E. Hamamsy","doi":"10.21608/aps.2019.10411.1003","DOIUrl":"https://doi.org/10.21608/aps.2019.10411.1003","url":null,"abstract":"Renal allograft survival requires the administration of multiple immunosuppressive drugs. This strategy may lead to gastric complications that necessitate gastro-protective medications, notably, proton pump inhibitors (PPIs). This study aimed to compare the effects of pantoprazole and esomeprazole on renal function in stable renal transplant recipients. A prospective, parallel, open-label clinical trial was performed with forty-seven adult renal transplant recipients receiving immunosuppressive therapy with cyclosporine (CSA) doses adjusted to attain trough concentrations of 100-150 µg/L, mycophenolate mofetil (MMF) at 750 mg q12 hr and prednisolone at 5 mg daily at Nasser Institute, Cairo, Egypt. The enrolled participants were randomized into two groups, which received either esomeprazole or pantoprazole at the same dose (40 mg once daily). Renal function was measured at baseline and monthly for 6 months. The study was conducted between January-September 2016. Main outcome measures clinical signs of rejection reflected by renal function decline, assessed by elevated levels of serum creatinine. The mean serum creatinine level was significantly lower in the sixth month than at baseline in esomeprazole group (p 0.004); interestingly there was a continuous decrease of serum creatinine levels in esomeprazole group and nearly constant values in pantoprazole group. There was no significant difference in serum creatinine levels between the two groups. From this study, it could be concluded that esomeprazole may be preferred over pantoprazole in renal transplant recipients because it decreased serum creatinine which is one of the markers of chronic allograft rejection in stable renal transplantation recipients.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90356443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Phytochemical Study on Olea europaea L. Olive Leaf Extract (cv. Koroneiki) growing in Egypt 油橄榄叶提取物的植物化学研究生长在埃及的Koroneiki

Archives of Pharmaceutical Sciences Ain Shams University Pub Date : 2019-01-01 DOI: 10.21608/aps.2019.45119

Doaa ElBohy, M. El Sharkawy, Soheir Abo-Elazm, S. Shahin, Waleed Bchari, Azza Manc, M. El Hamamsy

引用次数: 8

Oxaliplatin-Induced Peripheral Neuropathy Grade in Females with Colorectal Cancer Patients 奥沙利铂诱导的女性结直肠癌患者周围神经病变分级

Archives of Pharmaceutical Sciences Ain Shams University Pub Date : 2019-01-01 DOI: 10.21608/aps.2019.10411.2020

I. Ahmed, Lamia Elwakeel, Amr Shafik Tawfik, R. Abdel-Malek

{"title":"Oxaliplatin-Induced Peripheral Neuropathy Grade in Females with Colorectal Cancer Patients","authors":"I. Ahmed, Lamia Elwakeel, Amr Shafik Tawfik, R. Abdel-Malek","doi":"10.21608/aps.2019.10411.2020","DOIUrl":"https://doi.org/10.21608/aps.2019.10411.2020","url":null,"abstract":"The oxaliplatin-based regimen is considered an important standard of care for patients with colon cancer (CC). Neurotoxicity is the most relevant non-hematologic toxicity of oxaliplatin. The current study aimed to assess the association between colorectal cancer patient's (CRC) gender and susceptibility of oxaliplatin-induced sensory and motor peripheral neuropathy grades in Egyptian patients. All eligible patients between 18-75 years old with CRC were included in the study and only those who had a neurologic disease or diseases impairing neurologic function were excluded. Then eligible patients were stratified into two groups according to gender; oxaliplatin-induced peripheral neuropathy (OIPN) was evaluated and graded on day 1 of each chemotherapy cycle for 12 cycles according to the National Cancer Institute Common Terminology Criteria for Adverse Events (NCI-CTCAE), version 4.0 from March 2015 to October 2017. From a total of 47 females and 33 males; 41(87.2%) females and 29 (87.9%) males suffered from different grades of sensory and motor peripheral neuropathy.Regarding sensory oxaliplatin-induced peripheral neuropathy (OIPN), females had a higher risk of grade II-III PN than males (92.7% versus 58.6% respectively; p =0.001) For motor OIPN, females were more susceptible to grade II-III PN versus males (84.6% versus 56.5% respectively; p=0.015). In conclusion, High grade (grade II-III) Oxaliplatin-induced sensory and motor peripheral neuropathy occurred more frequently in females versus males Egyptian colorectal cancer patients.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89936201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina 微藻螺旋藻、斜螺旋藻和盐杜氏藻多糖的抗病毒、细胞毒、抗氧化和抗胆碱酯酶活性

Archives of Pharmaceutical Sciences Ain Shams University Pub Date : 2018-06-01 DOI: 10.21608/APS.2018.18740

A. Singab, N. Ibrahim, A. El-Sayed, W. El-Senousy, H. Aly, A. A. Elsamiae, A. Matloub

{"title":"Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina","authors":"A. Singab, N. Ibrahim, A. El-Sayed, W. El-Senousy, H. Aly, A. A. Elsamiae, A. Matloub","doi":"10.21608/APS.2018.18740","DOIUrl":"https://doi.org/10.21608/APS.2018.18740","url":null,"abstract":"Quantitative estimation of vegetative and stress forms of Spirulina platensis and Scenedesmus obliquus as well as vegetative form of Dunaliella salina revealed that S. obliquus constituted highest polysaccharide content than other tested microalgae. The isolated polysaccharides characterized as heterogeneous polysaccharides bounded protein by FT-IR, GLC and Elemental Microanalysis. These polysaccharides constituted of 47-66% of sugar and 14.88-41.06% of protein contents whereas galactose, mannose, glucose and rhamnose were represented as predominant sugar in isolated polysaccharides. The isolated polysaccharides were evaluated in vitro as antiviral, cytotoxic, antioxidant and anti-cholinesterase properties. The non-toxic dose of isolated polysaccharides on Huh 7.5, MA104, BGM and Vero cell lines were determined. The S. platensis (CEM and HEM) polysaccharides have promising antiviral which reduced replication up to 50–87.6% of HCV genotype 4a replicon, coxsackievirus B4, rotavirus and herpes simplex type 1 virus at non toxic doses 1.8 and 1.5 mg/ml, respectively. Furthermore, the isolated polysaccharides were assessed for in-vitro cytotoxic activity against MCF-7, HepG2 and HCT116 cell lines. The cytotoxic activity revealed that D. salina HEM polysaccharide show potent cytotoxic activity against HCT 116 cell line with IC50 64.2 μg/ml. Additionally, the isolated polysaccharides showed DPPH• scavenging activity in dose dependent relationship and D. salina HEM and S. obliquus CEM showed the significantly highest activity (308.16 and 308.69%, respectively) at 100 μg/ml. Furthermore, S. obliquus CEM and HEM polysaccharides exhibited the significant highest cholinestearse % inhibition activity. Microalgal polysaccharides have great therapeutically potential in drug development used as antiviral, antitumour, antioxidant and anticholinestrae agents in near future.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87572339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Assessing the impact of wastewater irrigation on some toxic heavy metals concentration in grains of Sorghum bicolor 废水灌溉对双色高粱籽粒中部分有毒重金属浓度的影响

Archives of Pharmaceutical Sciences Ain Shams University Pub Date : 2018-06-01 DOI: 10.21608/aps.2018.18739

Mohamed M. Taher

{"title":"Assessing the impact of wastewater irrigation on some toxic heavy metals concentration in grains of Sorghum bicolor","authors":"Mohamed M. Taher","doi":"10.21608/aps.2018.18739","DOIUrl":"https://doi.org/10.21608/aps.2018.18739","url":null,"abstract":"The impact of waste irrigation on the concentrations of the metals Cu, Fe, Ni, and Zn contents in grains of Sorghum bicolor was investigated through analyzing (for three seasons 2008-2010) four different-irrigation sorghum grains samples grown on four different-irrigation soil areas according to the experimental design. The two original soils: Soil, historically and experimentally, irrigated with Wastewater (SHEwastewater), and Soil, historically and experimentally, irrigated with wells water (SHEwells water), were analyzed in the 1st season. Wastewater and wells water samples were analyzed in the 1 st and 2 nd seasons. According to the procedures used in the literature, Samples were collected, pretreated, preserved, digested according to the microwave assisted acids digestion procedures, and analyzed for metals by ICP-AES. Quality control was performed and %R(s) we got were in the acceptable ranges for real samples analysis. Fe contents of the two types of soil samples were not significantly different. Cu and Ni contents of SHEwastewater were higher than that of SHEwells water whereas Zn content of SHEwastewater was lower than that of SHEwells water. In addition, not all metal levels of the two types of soil samples have exceeded the upper EU standards except nickel in SHEwastewater. The average means (of 1 and 2 seasons) levels of Cu, Fe, Ni of wastewater was higher than that of wells water, but for Zn, the case is reversed. In addition, the average means of all metal ions of both wastewater and wells water was lower than Yemeni standard for irrigation water. Sorghum grains analysis results, for at least two of the three seasons, indicated that: wastewater irrigation resulted in an increase of nickel content of grains of Sorghum bicolor. Wells water irrigation resulted in an increase in zinc content. In addition, there is no significant difference between the effect of wastewater and wells water irrigation on copper and iron contents of grains of Sorghum bicolor.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72592004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of amberlite XAD-2-ANSA resin for separation, preconcentration and spectrophotometric determination of trace elements in food samples 安贝岩XAD-2-ANSA树脂的合成及其分离、富集和分光光度法测定食品样品中微量元素

Archives of Pharmaceutical Sciences Ain Shams University Pub Date : 2018-06-01 DOI: 10.21608/aps.2018.18741

Mohamed M. Taher

{"title":"Synthesis of amberlite XAD-2-ANSA resin for separation, preconcentration and spectrophotometric determination of trace elements in food samples","authors":"Mohamed M. Taher","doi":"10.21608/aps.2018.18741","DOIUrl":"https://doi.org/10.21608/aps.2018.18741","url":null,"abstract":"A new chelating sorbent has been synthesized using Amberlite XAD-2 resin functionalized with 1-amino2-naphthol-4-sulfonic acid (ANSA) through –N=N– group. This sorbent, characterized by infrared (FTIR) spectra and optimized for sorption-desorption of the studied metal ions then, it was used as packing for the column for separation/ preconcentration of cadmium, cobalt, and nickel prior to their determination spectrophotometrically. Metal ions of Cd, Ni, and Co sorbed in the column were eluted one by one with 0.02 M, 0.10 M, and 0.20 M HCl acid respectively. Under the optimal conditions, the proposed procedure enabled the determination of Cd, Co, and Ni with detection limits of 0.98, 0.30 and 2.10 μg L− 1 respectively. The developed procedure was evaluated by the analysis of mixed metal ions Cd, Co and Ni solution contained 0.30, 30, and 1.25 μg respectively, and the percentage recovery was 95.5 – 104%. The procedure was applied to the analysis of food samples (grains of Sorghum bicolor), and the results obtained were compared with that of FAAS (before and after separation/preconcentration).","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85472010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of carbapenem resistance among multidrug-resistant Gram-negative uropathogens 多重耐药革兰氏阴性尿路病原菌中碳青霉烯耐药性的流行情况

Archives of Pharmaceutical Sciences Ain Shams University Pub Date : 2018-06-01 DOI: 10.21608/APS.2018.18736

A. Elshamy, K. Aboshanab, M. Yassien, N. Hassouna

{"title":"Prevalence of carbapenem resistance among multidrug-resistant Gram-negative uropathogens","authors":"A. Elshamy, K. Aboshanab, M. Yassien, N. Hassouna","doi":"10.21608/APS.2018.18736","DOIUrl":"https://doi.org/10.21608/APS.2018.18736","url":null,"abstract":"Multidrug-resistant (MDR) uropathogens have become a public health threat, especially in developing countries. Carbapenems are a class of antimicrobial agents often reserved for infections caused by MDR microorganisms. The aim of this study was to determine the prevalence and genotypic basis of plasmid-mediated carbapenem resistance among MDR uropathogens from one of the major clinical settings in Cairo, Egypt. A total of 150 bacterial isolates from patients suffering from urinary tract infections were collected from the Microbiology lab of El-Demerdash Hospital, Cairo, Egypt. All isolates were identified using standard methods. Antimicrobial susceptibility testing was carried out by Kirby Bauer's disk diffusion method following the CLSI guidelines. Plasmids were extracted from MDR uropathogens that also showed carbapenem resistance to be used as templates for PCR amplification. The resulting amplicons were subjected to DNA sequencing. The extracted plasmids were also transformed into Escherichia coli DH5α to compare the phenotypic resistance of the transformants with that of the clinical isolates from which the plasmids were extracted. Of the 150 collected isolates, 116 (77.3%) were Gram-negative, 51 of which (44%) were MDR. Carbapenem resistance was observed in 16/51 (31.4%) of the MDR isolates, 12 of which harbored plasmids. The blaOXA gene was detected in the plasmids of only 9 MDR carbapenem-resistant isolates. From this study, it can be concluded that Gram-negative uropathogens show high rates of multidrug-resistance. The prevalence of MDR uropathogens that are also carbapenem-resistant has increased greatly over the past few years, and this resistance can be easily acquired by horizontal transfer.","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87997645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Optimization of polyphenol extraction from Vitis vinifera L. leaves, antioxidant activity and its correlation with amelioration effect on AlCl3-induced Alzheimer’s disease 葡萄叶多酚提取工艺优化、抗氧化活性及其与alcl3诱导阿尔茨海默病改善作用的相关性

Archives of Pharmaceutical Sciences Ain Shams University Pub Date : 2018-06-01 DOI: 10.21608/APS.2018.18750

A. Matloub

{"title":"Optimization of polyphenol extraction from Vitis vinifera L. leaves, antioxidant activity and its correlation with amelioration effect on AlCl3-induced Alzheimer’s disease","authors":"A. Matloub","doi":"10.21608/APS.2018.18750","DOIUrl":"https://doi.org/10.21608/APS.2018.18750","url":null,"abstract":"This research focused on optimization of polyphenol extraction from Vitis vinifera L. (cv. Flame Seedless) leave based on 2,2-diphenyl-1-picrylhydrazyl (DPPH • ) scavenging capability in additional discussion of structure-activity relationship according to its amelioration effect on AlCl 3 -induced Alzheimer’s disease. The highest extraction yield was found for acidified aqueous solvents. While the highest total phenolic and flavonoid contents were found for aqueous solvents. The hydroacetonic extract showed significantly superior content in total phenolic and flavonoid accompanied by highest DPPH scavenging capability than other tested extracts. High-Performance Liquid Chromatography with Diode-Array Detection (HPLC/DAD) analysis of extracts led to identifying catechin, rutin, isoquercetin, quercitrin, apigenin-7-O -glucoside, quercetin, kaempferol and myricetin for the first time in V. vinifera L. (cv. Flame Seedless) leaves. In addition, quercetin-3-O - β -D-arabinopyranoside was isolated for the first time from V. vinifera L., detected as a major flavonoid in all extracts. This study emphasized the role of solvents played in polyphenol extraction and antioxidant activity from V. vinifera L. (cv. Flame Seedless) leaves. The hydroacetonic extract mainly composed of flavonoidal compounds which could ameliorate AlCl 3 -induced cerebral damages and neurocognitive dysfunction. This may lead to the development of new nutraceutical and pharmaceutical agents used as anti-oxidative stress and neuroprotective agent on Alzheimer and aging diseases","PeriodicalId":8314,"journal":{"name":"Archives of Pharmaceutical Sciences Ain Shams University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86223974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5