Archives internationales de physiologie et de biochimie最新文献

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Permeability properties of the Bufo bufo bladder as affected by isoprenaline and vasopressin. 异丙肾上腺素和利尿加压素对蟾蜍膀胱渗透性的影响。
Archives internationales de physiologie et de biochimie Pub Date : 1989-12-01 DOI: 10.3109/13813458909075085
C Lippe, C Ardizzone
{"title":"Permeability properties of the Bufo bufo bladder as affected by isoprenaline and vasopressin.","authors":"C Lippe,&nbsp;C Ardizzone","doi":"10.3109/13813458909075085","DOIUrl":"https://doi.org/10.3109/13813458909075085","url":null,"abstract":"<p><p>Isoprenaline, a beta adrenergic agonist, strongly increases both transepithelial fluxes across the urinary bladder of Bufo bufo; this effect is dose dependent, 10(-6)M being necessary for the maximal action. This effect is less selective than that of vasopressin: the ratio J urea/J thiourea is 3.8 under isoprenaline and 30.4 under vasopressin treatment. Both hormones differently affect the permeability of a mainly liposoluble molecule, i.e. antipyrine: vasopressin increases antipyrine permeability, while isoprenaline decreases it. Moreover diethylpyrocarbonate treatment of the luminal membrane strongly inhibits vasopressin effect on urea permeability leaving unmodified that of isoprenaline. However, the actions of both hormones are not additive. These results allows to assume that the tissue has a feedback mechanism which inhibits other hormonal action while the bladder is stimulated by a particular hormone.</p>","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"97 6","pages":"537-43"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813458909075085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13628038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Increased muscle regeneration after repair of divided motor nerve with neuronotrophic factors containing glue. 含胶神经营养因子修复分裂运动神经后增加肌肉再生。
Archives internationales de physiologie et de biochimie Pub Date : 1989-12-01 DOI: 10.3109/13813458909075083
C Becker, C Gueuning, A Gilbert, G L Graff
{"title":"Increased muscle regeneration after repair of divided motor nerve with neuronotrophic factors containing glue.","authors":"C Becker,&nbsp;C Gueuning,&nbsp;A Gilbert,&nbsp;G L Graff","doi":"10.3109/13813458909075083","DOIUrl":"https://doi.org/10.3109/13813458909075083","url":null,"abstract":"<p><p>Neuronotrophic factors (NTFs) directed to spinal cord motor neurons were collected in rats within silicone nerve regeneration chambers according to LONGO et al. (1983b). Unilateral addition of NTFs to the fibrin glue used for the repair of divided sciatic nerves improved locally nerve regeneration without affecting the controlateral side. Nerve regeneration was assessed by weight gain of the reinnervated muscles and by radioactive labelling of the acid-soluble phosphate fractions of both nerve Schwann cells and reinnervated muscle cells. Fast gastrocnemius and slow soleus muscles, the motor nerve of which had been repaired with added NTFs, were significantly heavier (21 and 28%) than their controlateral controls, and the metabolic dedifferentiation attendant on post-division nerve repair was less marked. It is suggested that this experimental nerve regeneration model is suitable to test potential nerve-active agents in vivo, under conditions close to the usual clinical setting, with, as ultimate goal, the improvement of the end-results of microsurgical repair of peripheral nerve in man.</p>","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"97 6","pages":"521-9"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813458909075083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13628036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Fatty acid binding proteins in bovine intestinal mucosa. 牛肠黏膜脂肪酸结合蛋白。
Archives internationales de physiologie et de biochimie Pub Date : 1989-12-01 DOI: 10.3109/13813458909075075
A Palacios, A Catalá
{"title":"Fatty acid binding proteins in bovine intestinal mucosa.","authors":"A Palacios,&nbsp;A Catalá","doi":"10.3109/13813458909075075","DOIUrl":"https://doi.org/10.3109/13813458909075075","url":null,"abstract":"<p><p>Cytosol obtained from bovine intestinal mucosa, contains two protein fractions that bind sulfobromophthalein and are able to remove [1-14C] palmitic acid from microsomal membranes. The high molecular weight protein fraction (F1) increases the binding of sulfobromophthalein 2 and 8 times respectively after heating at 60 degrees C during 5 min or delipidation. These changes do not correlate with the rate of palmitic acid removal from microsomes. F1 native or delipidated is more efficient than the low molecular weight protein (F2) on the removal of [1-14C]palmitic acid from microsomes. Two protein fractions DE-I and DE-II obtained from F1 by DEAE-cellulose chromatography have palmitic acid- and sulfobromophthalein-binding capacities respectively.</p>","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"97 6","pages":"441-6"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813458909075075","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13628760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Effects of temperature on the mitochondrial malate dehydrogenase of adult muscle of Toxocara canis. 温度对犬弓形虫成年肌肉线粒体苹果酸脱氢酶的影响。
Archives internationales de physiologie et de biochimie Pub Date : 1989-12-01 DOI: 10.3109/13813458909075076
E Mansini, E G Oestreicher, L P Ribeiro
{"title":"Effects of temperature on the mitochondrial malate dehydrogenase of adult muscle of Toxocara canis.","authors":"E Mansini,&nbsp;E G Oestreicher,&nbsp;L P Ribeiro","doi":"10.3109/13813458909075076","DOIUrl":"https://doi.org/10.3109/13813458909075076","url":null,"abstract":"<p><p>Purified mitochondrial malate dehydrogenase isoenzyme (m-MDH) of Toxocara canis muscle presented maximum activity at 48 degrees C. A clear change in slope of the Arrhenius plot was observed. The energy of activation calculated for the catalytic process showed values of 3.2 kcal/mol and 10.5 kcal/mol. Thermal inactivation of m-MDH showed that it is more thermolabile than the s-isoenzyme. The inactivation of the enzyme by heat could be reduced at least in part by the addition of 0.1 mM NADH. The heat denaturation showed to be a first-order process. The rate constant (k) was calculated as being of the order of 5.28 X 10(-4) s-1 at 40 degrees C. The activation energy for the heat inactivation process was 16.45 kcal/mol between 30 degrees C and 40 degrees C and 13.79 kcal/mol between 40 degrees C and 48 degrees C.</p>","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"97 6","pages":"447-53"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813458909075076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13628761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Alpha-adrenergic influences on exocrine pancreatic secretion in the rabbit. 肾上腺素能对兔外分泌胰腺的影响。
Archives internationales de physiologie et de biochimie Pub Date : 1989-12-01 DOI: 10.3109/13813458909075077
J J Calvo, I de Dios, M A Plaza, J I San Román, M A López
{"title":"Alpha-adrenergic influences on exocrine pancreatic secretion in the rabbit.","authors":"J J Calvo,&nbsp;I de Dios,&nbsp;M A Plaza,&nbsp;J I San Román,&nbsp;M A López","doi":"10.3109/13813458909075077","DOIUrl":"https://doi.org/10.3109/13813458909075077","url":null,"abstract":"<p><p>Action of phenylephrine (35 micrograms/Kg/min) alone or previously blocked by phentolamine (100 micrograms/Kg/min) on exocrine pancreatic secretion of anaesthetized rabbits has been studied, in basal state or under stimulation by secretin (1 C.U./Kg/h) or by the octapeptide of cholecystokinin (OP-CCK) (0.15 Ivy dog units/Kg/h). Phenylephrine increased arterial pressure. This effect was blocked by phentolamine. However no variations were seen in pancreatic blood flow in any of the experimental conditions assayed. Phenylephrine produced a secretin-like effect on hydroelectrolytic secretion in basal conditions. This action was maintained after the infusion of secretin but not after OP-CCK. This effect was not blocked by phentolamine. Phenylephrine increased protein secretion in the basal state, an action that was blocked by phentolamine. After secretin or OP-CCK stimulation phenylephrine did not increase protein secretion. It is concluded that phentolamine blocks the effects of phenylephrine on acinar cells but not on ductular cells.</p>","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"97 6","pages":"455-63"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813458909075077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13628762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Fundamental and applied aspects of nutritional and transport processes. Abstracts of the 11th Conference of the European Society for Comparative Physiology and Biochemistry and the 1st Joint Meeting ESCPB-Association des Physiologistes. September 3-7, 1989, Reims. 营养和运输过程的基本和应用方面。第11届欧洲比较生理与生物化学学会会议暨第1届欧洲比较生理与生物化学学会联席会议摘要。1989年9月3日至7日,兰斯。
{"title":"Fundamental and applied aspects of nutritional and transport processes. Abstracts of the 11th Conference of the European Society for Comparative Physiology and Biochemistry and the 1st Joint Meeting ESCPB-Association des Physiologistes. September 3-7, 1989, Reims.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"97 5","pages":"C1-55"},"PeriodicalIF":0.0,"publicationDate":"1989-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Relationship between the 4 mmol running velocity, the time-distance relationship and the Léger-Boucher's test. 4 mmol跑速、时距关系与lsamger - boucher试验的关系。
Archives internationales de physiologie et de biochimie Pub Date : 1989-10-01 DOI: 10.3109/13813458909104547
J M Lechevalier, H Vandewalle, J C Chatard, A Moreaux, V Gandrieux, F Besson, H Monod
{"title":"Relationship between the 4 mmol running velocity, the time-distance relationship and the Léger-Boucher's test.","authors":"J M Lechevalier,&nbsp;H Vandewalle,&nbsp;J C Chatard,&nbsp;A Moreaux,&nbsp;V Gandrieux,&nbsp;F Besson,&nbsp;H Monod","doi":"10.3109/13813458909104547","DOIUrl":"https://doi.org/10.3109/13813458909104547","url":null,"abstract":"<p><p>The relationship between distance and best time is roughly linear for distances between 1500 and 5000 m. The slope of this relationship has the dimension of a velocity (Vlim) which can be sustained during a long time. The individual time-distance relationships and the resulting Vlim have been studied in 32 subjects practicing different athletic activities by measuring exhaustion time for 2 to 4 constant-velocity running exercises performed to exhaustion. The velocity corresponding to 4 mmol.l-1 of blood lactate (V4 mmol) has been compared with Vlim. As maximal oxygen uptake is a major factor determining V4 mmol, Vlim and V4 mmol have also been correlated with the result of a field test which is assumed to measure maximal aerobic power (Léger-Boucher's test). This test consists in running until exhaustion at a velocity which increases every two minutes. The higher the velocity at exhaustion (Vléger) is, the higher the maximal oxygen uptake is assumed. Both Vlim and Vléger were very well correlated with V4 mmol (r greater than 0.90) and the average value of Vlim was almost equal to the average value of V4 mmol (13.89 vs 13.71 km.h-1). However, it was not possible to estimate V4 mmol accurately from the values of Vlim or Vléger because the standard errors of estimates were too large.</p>","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"97 5","pages":"355-60"},"PeriodicalIF":0.0,"publicationDate":"1989-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813458909104547","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 28
Effects of bupivacaine on the membrane properties of nerve cell soma. 布比卡因对神经细胞体膜性质的影响。
Archives internationales de physiologie et de biochimie Pub Date : 1989-10-01 DOI: 10.3109/13813458909104554
M Tabatabai, A M Booth, B Kirimli
{"title":"Effects of bupivacaine on the membrane properties of nerve cell soma.","authors":"M Tabatabai,&nbsp;A M Booth,&nbsp;B Kirimli","doi":"10.3109/13813458909104554","DOIUrl":"https://doi.org/10.3109/13813458909104554","url":null,"abstract":"<p><p>While the effects of local anaesthetics on axonal conduction and axonal membrane have been extensively studied, there is little information about the actions of these agents on nerve cell soma. Therefore, the effects of the amide local anaesthetic bupivacaine on the electrophysiologic properties of the nerve cell soma were studied on isolated superfused superior cervical ganglia of rats. Administration of 100-200 nM of bupivacaine to the preparation produced marked changes in membrane properties of the cell soma. The resting membrane potential did not change, but the membrane resistance decreased 20% (P less than 0.01). The firing threshold, the action potential duration at 50% of maximal amplitude, and the intracellular current threshold for firing the cells increased significantly (P less than 0.01), while the action potential amplitude decreased significantly (P less than 0.01), before its complete blockade. The results show that the cell soma is a major site of action of local anaesthetics. The implication of the results is that when local anaesthetics are applied to areas where cell bodies and processes (axons and dendrites) are present together, such as during celiac plexus block, lumbar sympathetic block, stellate ganglion block, etc., they will all be effectively depressed and/or blocked.</p>","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"97 5","pages":"409-15"},"PeriodicalIF":0.0,"publicationDate":"1989-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813458909104554","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13625241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Alterations of energy metabolism induced by hexadecane in mice. 十六烷对小鼠能量代谢的影响。
Archives internationales de physiologie et de biochimie Pub Date : 1989-10-01 DOI: 10.3109/13813458909104544
J Huguet, J Cartaña, L Arola, M Alemany
{"title":"Alterations of energy metabolism induced by hexadecane in mice.","authors":"J Huguet,&nbsp;J Cartaña,&nbsp;L Arola,&nbsp;M Alemany","doi":"10.3109/13813458909104544","DOIUrl":"https://doi.org/10.3109/13813458909104544","url":null,"abstract":"<p><p>Two groups of young male OF-1 mice were fed for 60 days with cafeteria or, as controls, with standard pellet diet respectively. At that time, both groups were daily treated with hexadecane (HDK) on the skin. HDK induced a drastic body weight loss much higher in cafeteria than control mice. White adipose tissue were exhausted after 4 days of treatment in controls but not after 10 days in cafeteria ones. HDK resulted in mobilization of liver glycogen in both groups while muscle glycogen decreased slightly in the end. Hexadecane treatment did not result in massively enhanced nitrogen metabolism, as the actual oxidation of amino acids decreased considerably as indicated by the low levels of plasma urea. The results could be explained by powerful and lasting effects of hexadecane on thermogenesis and metabolic reserve balance. The use of this material for pharmacological manipulation of body weight appeared difficult.</p>","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"97 5","pages":"333-40"},"PeriodicalIF":0.0,"publicationDate":"1989-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813458909104544","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Effect of hypo and hyperprolactinemia on the testicular maturation of Wistar rats during puberal transition. 低、高催乳素血症对青春期过渡期Wistar大鼠睾丸成熟的影响。
Archives internationales de physiologie et de biochimie Pub Date : 1989-10-01 DOI: 10.3109/13813458909104546
M Prasad, G Uma Devi, S Govindappa
{"title":"Effect of hypo and hyperprolactinemia on the testicular maturation of Wistar rats during puberal transition.","authors":"M Prasad,&nbsp;G Uma Devi,&nbsp;S Govindappa","doi":"10.3109/13813458909104546","DOIUrl":"https://doi.org/10.3109/13813458909104546","url":null,"abstract":"<p><p>In Wistar rats, the structural and metabolic organization of the testis was influenced by the blood concentration of prolactin. The androgen dependent enzyme activities in plasma as well as in testis were higher under hyperprolactinemia and lower under hypoprolactinemia, as induced by bromocriptine. While prolactin had direct effect on the testicular functions, bromocriptine seemed to exert its influence through blocking hypophysial prolactin.</p>","PeriodicalId":8170,"journal":{"name":"Archives internationales de physiologie et de biochimie","volume":"97 5","pages":"347-53"},"PeriodicalIF":0.0,"publicationDate":"1989-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/13813458909104546","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13624155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
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