Archives of Clinical Microbiology最新文献_第10页

In vivo Antimicrobial Activity ofOzonized Theobroma Oil Ovulesagainst Candida albicans 臭氧化可可油胚珠对白色念珠菌的体内抑菌活性

Archives of Clinical Microbiology Pub Date : 2017-01-01 DOI: 10.4172/1989-8436.100070

Maritza Fd, R. Meneau, Fernández, Yaima Sánchez, G. García

{"title":"In vivo Antimicrobial Activity ofOzonized Theobroma Oil Ovulesagainst Candida albicans","authors":"Maritza Fd, R. Meneau, Fernández, Yaima Sánchez, G. García","doi":"10.4172/1989-8436.100070","DOIUrl":"https://doi.org/10.4172/1989-8436.100070","url":null,"abstract":"Title: Effects of ozonated theobroma oil and ketoconazole ovules on rats infected with Candida albicans. Background: Candidiasis is an infection caused by a yeast shaped fungus which includes Candida gender. Candida albicans is an opportunistic microorganism, which causes more than 80% of vaginal infections. The aim of this study was to determine the effect of ozonized theobroma oil vaginal ovules in the treatment of induced vaginal candidiasis compared to Ketoconazole ovules in Sprague Dawley female rats. Methods and Findings: Animals were ovariectomized and injected with a hormonal treatment after 14 days in order to know the oestrus cycle. After 48 hours rats with keratinous cellules were vaginally infected with an inoculum of 106-107 Candida albicans in 0.1 mL of phosphate buffer solution. Five animals groups were studied: group I (without treatment), group II (treated with unozonized theobroma oil ovules), group III (treated with ketoconazole ovules), group IV (treated with 10% ozonized theobroma oil ovules), and group V (treated with 20% ozonized theobroma oil ovules). Exudates were made before beginning the treatment, 5 and 10 days during the treatment and 48 hours after the end of treatment. Results demonstrated a decrease of 0.7 log of the number of rats with infection after 5 days of treatment with 20% ozonized theobroma oil ovules; however, it was not observed infection in rats after 10 days. A similar result was obtained with ketoconazole ovules. Conclusions: Due to antimicrobial activity of 20% ozonized theobroma oil ovules, it can be recommended their use for treatment of Candidiasis","PeriodicalId":8142,"journal":{"name":"Archives of Clinical Microbiology","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84994332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mycobacterium bovis Prevalence in Humans Does Not Differ between Regions in Burkina Faso 在布基纳法索，牛分枝杆菌在人类中的流行率在不同地区之间没有差异

Archives of Clinical Microbiology Pub Date : 2017-01-01 DOI: 10.4172/1989-8436.100065

S. Diagbouga, C. Nadembèga, Z. Tarnagda, Arthur Djibougou, N. Henry, R. Bicaba, M. Dembelé, Adjima Combary, Souba Di, M. Ouédraogo, G. Aurégan, P. Perre

{"title":"Mycobacterium bovis Prevalence in Humans Does Not Differ between Regions in Burkina Faso","authors":"S. Diagbouga, C. Nadembèga, Z. Tarnagda, Arthur Djibougou, N. Henry, R. Bicaba, M. Dembelé, Adjima Combary, Souba Di, M. Ouédraogo, G. Aurégan, P. Perre","doi":"10.4172/1989-8436.100065","DOIUrl":"https://doi.org/10.4172/1989-8436.100065","url":null,"abstract":"Background: In 1996, tuberculosis detection rate was higher in Oudalan (Sahel region) than in other provinces in Burkina Faso. Cattle breeding, a major activity of the local population, may favor exposure of humans to bovine tuberculosis. Objectives: (i) to isolate and identify M. bovis among new cases of tuberculosis with positive culture in the Sahel (Gorom-Gorom and Dori); the Center (Ouagadougou) and the Western (Bobo-Dioulasso) regions of the country; (ii) to investigate susceptibility of isolated strains to anti-tuberculosis. Methods: (i) collection of sputum from newly TB patients with a positive smear during a prospective study carried out in 1998 in the North, Central and Western region of the country and transferred at the Mycobacteriology Laboratory, (ii) optimization of operational procedures: (iii) mycobacteria strains's isolation on 3 different media (Loweinstein Jensen (LJ), LJ without glycerine and LJ supplemented with pyruvate; (iv) identification of the tuberculosis complex according to criteria such as: bacillus growth time, colony appearance, biochemical test results, bacilli growth with pyrazinamide or thiophene carboxylate hydrazide, or D-cycloserine; (v) performing antibiograms. Results: Among the isolated strains, M. bovis represented 6.2% (2/30) in Gorom-Gorom, 0% (0/18) in Dori, 2.6% (3/109) in Ouagadougou and 2.9% (2/65) in Bobo-Dioulasso. Sixty nine percent (31/45) of strains were susceptible to the main anti-tuberculosis drugs, 13 strains presented single or combined resistance and one strain was multidrug-resistant. Conclusion: We showed that M. bovis disease is prevalent in all the studied regions but at a relatively low rate. The transhumance of from the north to the west practiced by the breeders could explain why it was found M. bovis in a similar rate as in Ouagdougou and Bobo-Dioulasso where livestock activity is less intense than in Gorom-Gorom.","PeriodicalId":8142,"journal":{"name":"Archives of Clinical Microbiology","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82047729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Target Animal Safety and Residual Studyfor Berberine and other PhytogenicCompounds in Broiler Chickens 肉鸡小檗碱及其它植物原性化合物的靶动物安全性及残留研究

Archives of Clinical Microbiology Pub Date : 2017-01-01 DOI: 10.4172/1989-8436.100069

D. X. Yu, Zhiyong He, C. Pouton, F. Hoerr, Zhi-Cheng Xiao

{"title":"Target Animal Safety and Residual Studyfor Berberine and other PhytogenicCompounds in Broiler Chickens","authors":"D. X. Yu, Zhiyong He, C. Pouton, F. Hoerr, Zhi-Cheng Xiao","doi":"10.4172/1989-8436.100069","DOIUrl":"https://doi.org/10.4172/1989-8436.100069","url":null,"abstract":"Background: Phytogenic compounds are one of many alternatives to current industry feed ingredients for the poultry industry. However, despite increased reports on the activity of phytogenic compounds against disease in poultry, there is a lack of data regarding safety and residual toxicity. This two-part study investigated the general health of broiler chickens fed Berberine, Ursolic Acid, Piceid, Honokiol and Baicalin in-feed at three different levels, and assessed the poultry tissue residue of Berberine in breast muscle, upper and lower thigh, and liver through LC/MS-MS. Methods and Findings: Phase 1 trial results demonstrated that all birds appeared normal, with all gastrointestinal histologic lesions and liver histologic lesions identified within normal limits for broiler chickens in a production environment. Phase 2 trial showed that the lowest amount of Berberine, 0.03 g/kg in-feed, resulted in tissue residues below the lower limit of detection (<2 ng/g), and the highest amount of Berberine, 0.3 g/kg in-feed, yielded detectable values, although the significance of these results is still not clear. Conclusions: In conclusion, the present study suggests that the five tested phytogenic compounds are safe for use in starter, grower and finisher feeds for broiler chickens. Further exposure and risk assessment calculations regarding appropriate residue levels are necessary to evaluate the use of Berberine as a feed ingredient for poultry.","PeriodicalId":8142,"journal":{"name":"Archives of Clinical Microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75937086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Antimicrobial and Preservative Activities of Lippia Multiflora Essential Oil on Smoked Mackerel (Scomber Scombrus) Fish 多花Lippia精油对烟熏鲭鱼的抗菌和防腐作用

Archives of Clinical Microbiology Pub Date : 2017-01-01 DOI: 10.4172/1989-8436.100063

G. Cyrille, D. Adjéhi, S. Yaya, K. Désiré, Kassi Amian Brise Benjamin, D. Marcellin

{"title":"Antimicrobial and Preservative Activities of Lippia Multiflora Essential Oil on Smoked Mackerel (Scomber Scombrus) Fish","authors":"G. Cyrille, D. Adjéhi, S. Yaya, K. Désiré, Kassi Amian Brise Benjamin, D. Marcellin","doi":"10.4172/1989-8436.100063","DOIUrl":"https://doi.org/10.4172/1989-8436.100063","url":null,"abstract":"Objectives: The present study aims to exploit antimicrobial potentialities of the essential oil of Lippia multiflora in preserving smoked mackerel fish. Methods and findings: Essential oil extracted from Lippia multiflora was applied on smoked mackerel (Scomber scombrus) fish samples obtained from smoking and selling outlets in Yamoussoukro metropolis (Cote d'Ivoire). Microbiological quality determinations include microbial load counts, using enumerations of total aerobic mesophilic flora, total coliforms, yeasts & moulds and Staphylococcus aureus, before and after application of essential oil, and during fish samples preservation studies. The contamination levels of market smoked fish samples were total aerobic mesophilic flora (4.3 log10 CFU/g); total coliforms (1.42 log10 CFU/g); yeasts & moulds (2.45 log10 CFU/g) and Staphylococcus aureus (1.41 log10 CFU/g). There were no microbial loads recorded for the freshly smoked mackerel fish samples. One ml of L. multiflora essential oil added to 70 g of fish samples inhibited the growth of total aerobic mesophilic flora by 100% in 24 h, while the smoked fish samples were preserved for 3 days without repeated smoking. Conclusion: L. multiflora essential oil can serve as a bio-preservative for smoked mackerel fish instead of repeated smoking or chemical preservation.","PeriodicalId":8142,"journal":{"name":"Archives of Clinical Microbiology","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91299920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Simultaneous Detection of BacterialMeningitis in Suspected Cases ofMeningitis in Children Using PCR Assay,In Taif, Saudi Arabia PCR法同时检测沙特阿拉伯塔伊夫儿童疑似脑膜炎病例中的细菌性脑膜炎

Archives of Clinical Microbiology Pub Date : 2017-01-01 DOI: 10.4172/1989-8436.100048

N. Mohamed, A. Abubokr

{"title":"Simultaneous Detection of BacterialMeningitis in Suspected Cases ofMeningitis in Children Using PCR Assay,In Taif, Saudi Arabia","authors":"N. Mohamed, A. Abubokr","doi":"10.4172/1989-8436.100048","DOIUrl":"https://doi.org/10.4172/1989-8436.100048","url":null,"abstract":"Background: In vitro cultivation of salivarian species of trypanosomes requires the use of CO2-incubator, expensive equipment which relies solely on steady electricity supply. A simple culture system which uses glass desiccator gassed with laboratory-prepared carbon dioxide (5CG-Desiccator) was found to effectively sustain trypanosomes growth in vitro and so the technique was recommended for in vitro antitrypanosomal screening of compounds and plant extracts. This paper reports an attempt to evaluate the utility of this system for the determination of IC50, IC90 values of ethanolic extracts from some medicinal plants against Trypanosoma brucei brucei using Diminazine Aceturate (DA) as standard trypanocide. Methods findings: Parasites were grown in Eagle’s Medium in the presence of varying concentrations of each plant extract in a 96-well micro-titer plate using 5CGDesiccator culture method. IC50 and IC90 values were extrapolated from two graphical approaches. Comparative analysis of IC90 values showed that extracts from four plants (Cymbopogon spp, Moringa oleifera, Vernonia amygdalina and Allium sativum) had IC90 values less than 200 g/ml, five (Azadirachta indica, Khaya senegalensis, Carica papaya, Eucalyptus spp and Aloe vera) had values between 200 g/ml and 1000 g/ml and one (Mitracarpus scaber) had value greater than 1000 g/ml. Conclusion: The results obtained using two different approaches permitted the comparative assessment of the trypanocidal potentials of the extracts. Suggestions on practical ways to improve this technique for IC50 determination of values of synthetic and plant-derived compounds/fractions were discussed.","PeriodicalId":8142,"journal":{"name":"Archives of Clinical Microbiology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78225244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A Severe Leptospira interrogans SerovarCopenhageni Infection Diagnosed by Next-Generation Sequencing and Treated withCorticosteroids 新一代测序诊断1例严重钩端螺旋体血清哥本哈根感染并用皮质类固醇治疗

Archives of Clinical Microbiology Pub Date : 2017-01-01 DOI: 10.4172/1989-8436.100043

W. Zeng, L. Liqiang, B. Yuhai, W. Fang, T. Jiansong, J. Yuan, Ji, L. Ong, L. Jianming, Z. Rongrong, N. Pei, J. Huijue, X. Liu, H. Wang, M. Jinmin, Yingxiao Liu

{"title":"A Severe Leptospira interrogans SerovarCopenhageni Infection Diagnosed by Next-Generation Sequencing and Treated withCorticosteroids","authors":"W. Zeng, L. Liqiang, B. Yuhai, W. Fang, T. Jiansong, J. Yuan, Ji, L. Ong, L. Jianming, Z. Rongrong, N. Pei, J. Huijue, X. Liu, H. Wang, M. Jinmin, Yingxiao Liu","doi":"10.4172/1989-8436.100043","DOIUrl":"https://doi.org/10.4172/1989-8436.100043","url":null,"abstract":"Introduction: Leptospirosis is a worldwide zoonotic disease, with variable severity and involvement of multiple organs. Severe leptospirosis is associated with high morbidity and mortality rates, which poses a great threat to human health. Due to the limitation of traditional clinical and laboratory diagnosis methods, pathogens for many infectious diseases, including leptospirosis, remain unknown. Recently, unbiased next generation sequencing (NGS) sheds light on the precise and rapid diagnosis of intractable infectious diseases, which is greatly beneficial to the prompt treatment and reliable prognosis. Case presentation: A 35 year old male got a fever with rapid hepato-renal-pulmonary involvement after his travel to Hunan province, China. The manifestations initiated with a jaundice-hemorrhage clinical type, and then followed by the combination of jaundice-hemorrhage and diffuse hemorrhage (PDH) clinical types, along with liver, heart, muscle and pancreas involvement. To identify the causative agent for the disease, blood sample was collected for serologicaltests, pathogen-specific PCR detection. Meanwhile, blood and sputum samples were used to do the nextgeneration sequencing, and the Leptospira interrogans serovar Copenhageni was detected and confirmed as the pathogenic agent. Based on the precise diagnosis, our patient recovered progressively with antimicrobials and supportive care. Conclusion: NGS played a critical role in the precise diagnosis of leptospirosis infection in this case. Corticosteroids combined with antibiotics treatments based on the diagnosis and clinical signs with suspicious pulmonary hemorrhage may decelerate the disease progression. The unbiased NGS would be further used in clinics for disease diagnosis especially for the uncertain and unknown pathogens.","PeriodicalId":8142,"journal":{"name":"Archives of Clinical Microbiology","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90357297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Assessment of Sputum Quality and Its Importance in the Rapid Diagnosis of Pulmonary Tuberculosis 痰液质量评价及其在肺结核快速诊断中的意义

Archives of Clinical Microbiology Pub Date : 2017-01-01 DOI: 10.4172/1989-8436.100053

L. MercyAparna, S. Aparna, I. Sarada, D. Ram

{"title":"Assessment of Sputum Quality and Its Importance in the Rapid Diagnosis of Pulmonary Tuberculosis","authors":"L. MercyAparna, S. Aparna, I. Sarada, D. Ram","doi":"10.4172/1989-8436.100053","DOIUrl":"https://doi.org/10.4172/1989-8436.100053","url":null,"abstract":"Background: Globally, Tuberculosis (TB) still remains a major public health problem. In India, Pulmonary Tuberculosis (PTB) is the most common form of the disease; however, Extrapulmonary Tuberculosis (EPTB) comprises 10 to 20% of all TB cases. The diagnosis of EPTB cases is difficult because of paucibacillary nature and consequently is associated with low sensitivity of Zhiel- Neelson (ZN) smear and culture on Lowenstein-Jensen (LJ) media as gold standard. The present study comparatively evaluates the utility of real time quantitative PCR over nested PCR and other conventional techniques for the detection of M. tuberculosis Complex (Mtc) in clinical isolates of PTB and EPTB samples at a tertiary care centre, Shri Ram Murti Smarak Institute of Medical Sciences (SRMSIMS), Bareilly. Methods and Findings: In total, 205 both pulmonary (24) and extrapulmonary (181) specimens were processed for ZN smear, culture on LJ media, nested PCR and real time quantitative PCR using IS6110 as a gene target. Out of 205 samples, none of the sample was found to be smearpositive and only 28 (14%) samples were found to be culture positive. The nested PCR and real time quantitative PCR positivity was observed 100% in culture positive specimens. However in majority of culture negative specimens the sensitivity was 100% in both nested PCR and real time quantitative PCR assays for EPTB and PTB specimens. The specificity was better in case of real time quatitative PCR as compared to nested PCR, 45.8% and 20% in case of nested PCR for nonrespiratory and respiratory specimens respectively. The specificity was increased upto 86.1 and 100% with real time quantitative PCR for EPTB and PTB cases respectively. Conclusions: The combined analysis of nested PCR, real time quantitative PCR and other lab investigations can be very useful in the rapid diagnosis of M. tuberculosis in paucibacillary extrapulmonary tuberculosis samples in Indian scenario.","PeriodicalId":8142,"journal":{"name":"Archives of Clinical Microbiology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73776236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Interaction of Helicobacter Pylori with Other Microbiota Species in the Development of Gastric Cancer 幽门螺杆菌与其他微生物群在胃癌发生中的相互作用

Archives of Clinical Microbiology Pub Date : 2017-01-01 DOI: 10.4172/1989-8436.100067

Xiaoling Liu, Weiwei Nie, Jing Liang, Yan Li

{"title":"Interaction of Helicobacter Pylori with Other Microbiota Species in the Development of Gastric Cancer","authors":"Xiaoling Liu, Weiwei Nie, Jing Liang, Yan Li","doi":"10.4172/1989-8436.100067","DOIUrl":"https://doi.org/10.4172/1989-8436.100067","url":null,"abstract":"Background/aim: The increasing number of multidrug-resistant (MDR) bacteria and the need to synthesize new antimicrobials, nanoparticles have attracted interest in the scientific community. This study aimed to evaluate the synergistic effect of nanoparticles with some antibiotics against MDR pathogenic bacteria. Material and methods: Seventeen bacterial isolates including; Escherichia coli (n=5) and Klebsiella pneumoniae (n=12) were collected from different clinical samples of patients suffering from burn wound and fistulae wounds infections. The susceptibility of the collected isolates to different antibiotics was investigated. Six MDR isolates were selected as representatives to different patterns were used for the determination of the antibacterial activities of two metals nanoparticles; silver (Ag-NPs) and zinc oxide (ZnO-NPs). The antibacterial activities of the synthesized nanoparticles alone and their combination with the selected antibiotics were determined against the tested isolates using disc-diffusion method according to EUCAST Clinical Breakpoint. Results: Well dispersed spherical Ag-NPs with average particle size 12.65 ± 0.55 nm and ZnO-NPs of 7.6 ± 0.5 nm were chemically synthesized by the chemical reduction method and chemical precipitation method, respectively. The obtained results suggested that the Ag-NPs alone had higher antibacterial activity than ZnO-NPs. Furthermore, Ag-NPs show an observable synergistic effect with some antibiotics; more than ZnO-NPs. Conclusions: It was concluded that Ag-NPs have potential as a combination therapeutic agent for the treatment of infectious diseases by bacteria.","PeriodicalId":8142,"journal":{"name":"Archives of Clinical Microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79794515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Sickle Cell Sperm Selection with Hb-S Mab: A Future Application for Intracytoplasmic Genotypically Selected Sperm Injection (IGSI) Hb-S单抗镰状细胞精子选择:在胞浆内基因型选择精子注射(IGSI)中的应用

Archives of Clinical Microbiology Pub Date : 2017-01-01 DOI: 10.4172/1989-8436.100064

O. Adenmosun, W. Asghar, J. Kumi-Diaka

{"title":"Sickle Cell Sperm Selection with Hb-S Mab: A Future Application for Intracytoplasmic Genotypically Selected Sperm Injection (IGSI)","authors":"O. Adenmosun, W. Asghar, J. Kumi-Diaka","doi":"10.4172/1989-8436.100064","DOIUrl":"https://doi.org/10.4172/1989-8436.100064","url":null,"abstract":"The Current study revealed the natural occurrence of toxigenic fungi and mycotoxins production in grains in Saudi Arabia. Samples of yellow corn, white rice and red corn grains were collected from different local markets. Three fungal isolates were isolated from the examined corn grains using PDA media. Alternaria sp were the most prominent fungi in yellow corn grains, white rice and red corn grains. Three Alternaria sp isolated were identified using molecular characterization of Actts gene. DNA genome of the three Alternaria sp isolates (namely AWR; AYC and ARC which corresponds to isolates from white rice, yellow corn, red corn) was used as a template for PCR to amplify Actts gene. Partially sequenced Actts gene was amplified using a specific primer set to confirm its identity, phylogenetic relationships between the three isolates as well as determination of the corresponding antigenic determinants. The epitope prediction analysis demonstrated that there were 5, 6 and 5 epitopes whose score were above 0.90 in AWR, AYC and ARC, respectively. Interestingly, there were great variations in the epitope sequences among the three isolates except for the epitope, VYGASTATGTLAVQ. This work led to molecular identification of three Alternaria sp. using Actts toxin gene and the unique antigenic determinants that could be used for design of a broad spectrum antibody for rapid detection of Alternaria sp. in foods.","PeriodicalId":8142,"journal":{"name":"Archives of Clinical Microbiology","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90353977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Susceptibility of Beta-Haemolytic Escherichia coli to Commonly Used Antibiotics in Selected Hospitals in Delta State, Southern Nigeria 尼日利亚南部三角洲州选定医院中β -溶血性大肠杆菌对常用抗生素的敏感性

Archives of Clinical Microbiology Pub Date : 2017-01-01 DOI: 10.4172/1989-8436.100066

I. S. Aghemwenhio, A. Timilehin, Alpheus Ga

{"title":"Susceptibility of Beta-Haemolytic Escherichia coli to Commonly Used Antibiotics in Selected Hospitals in Delta State, Southern Nigeria","authors":"I. S. Aghemwenhio, A. Timilehin, Alpheus Ga","doi":"10.4172/1989-8436.100066","DOIUrl":"https://doi.org/10.4172/1989-8436.100066","url":null,"abstract":"Antimicrobial resistance in the treatment of urinary tract infections is a major health problem. This study evaluates the pattern of susceptibility of pathogens commonly responsible for urinary tract infections (UTIs) to commonly used antimicrobial agents. Midstream urine samples of 80 patients (30 males and 50 females) and 20 diarrhea samples from 10 males and 10 females, who were attending clinics in Eku, Sapele and Abraka general hospitals, Delta state, between September and October 2007 were examined. Susceptibility of the urine and diarrhea bacteria isolates to ten commonly used antibiotics were investigated. Thirty five Escherichia coli isolates were obtained from the urine samples, out of which were nine hemolytic strains (25.7%), and 10 E. coli isolates from diarrhea, out of which was one strain (10%). All the hemolytic strains exhibited a significantly high resistance to septrin, sparfloxacin, amoxicillin, streptomycin, chloramphenicol but were either moderately or highly sensitive to the augmentin and gentamycin. I conclude that the incidence of hemolytic strains in urinary tract infection was higher than that observed from diarrhea because Escherichia coli strains that cause urinary tract infection typically produce hemolysins which is an important virulent factorin pathogenesis of infection, whereas those strains that are part of the gastro intestinal micro flora may not produce hemolysins and majority of the antimicrobial agents that are commonly used to treat UTIs caused by Escherichia coli in the hospitals are no longer effective due to hemolysin production. Therefore, the development and strict management of antimicrobial policy, and surveillance for resistant organisms should be given priority in Nigeria.","PeriodicalId":8142,"journal":{"name":"Archives of Clinical Microbiology","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89975575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10