Aquatic Microbial Ecology最新文献_第7页

Diel oscillations in the feeding activity of heterotrophic and mixotrophic nanoplankton in the North Pacific Subtropical Gyre 北太平洋副热带环流异养和混合营养纳米浮游生物摄食活动的昼夜振荡

IF 1.4 4区环境科学与生态学

Aquatic Microbial Ecology Pub Date : 2020-12-03 DOI: 10.3354/ame01950

Paige E. Connell, F. Ribalet, E. Armbrust, A. White, D. Caron

{"title":"Diel oscillations in the feeding activity of heterotrophic and mixotrophic nanoplankton in the North Pacific Subtropical Gyre","authors":"Paige E. Connell, F. Ribalet, E. Armbrust, A. White, D. Caron","doi":"10.3354/ame01950","DOIUrl":"https://doi.org/10.3354/ame01950","url":null,"abstract":"Daily oscillations in photosynthetically active radiation strongly influence the timing of metabolic processes in picocyanobacteria, but it is less clear how the light−dark cycle affects the activities of their consumers. We investigated the relationship between marine picocyanobacteria and nanoplanktonic consumers throughout the diel cycle to determine whether heterotrophic and mixotrophic protists (algae with phagotrophic ability) display significant periodicity in grazing pressure. Carbon biomass of Prochlorococcus and Synechococcus was estimated continuously from abundances and cell size measurements made by flow cytometry. Picocyanobacterial dynamics were then compared to nanoplankton abundances and ingestion of fluorescently labeled bacteria measured every 4 h during a 4 d survey in the North Pacific Subtropical Gyre. Grazing of the labeled bacteria by heterotrophic nanoplankton was significantly greater at night than during the day. The grazing activity of mixotrophic nanoplankton showed no diel periodicity, suggesting that they may feed continuously, albeit at lower rates than heterotrophic nanoplankton, to alleviate nutrient limitation in this oligotrophic environment. Diel changes in Prochlorococcus biomass indicated that they could support substantial growth of nanoplankton if those grazers are the main source of picocyanobacterial mortality, and that grazers may contribute to temporally stable abundances of picocyanobacteria.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"4 1","pages":"167-181"},"PeriodicalIF":1.4,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74685815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Natural chemical control of marine associated microbial communities by sessile Antarctic invertebrates 南极无脊椎动物对海洋伴生微生物群落的自然化学控制

IF 1.4 4区环境科学与生态学

Aquatic Microbial Ecology Pub Date : 2020-12-03 DOI: 10.3354/ame01948

C. Angulo–Preckler, E. García-López, B. Figuerola, C. Avila, C. Cid

{"title":"Natural chemical control of marine associated microbial communities by sessile Antarctic invertebrates","authors":"C. Angulo–Preckler, E. García-López, B. Figuerola, C. Avila, C. Cid","doi":"10.3354/ame01948","DOIUrl":"https://doi.org/10.3354/ame01948","url":null,"abstract":"Organisms living in the sea are exposed to fouling by other organisms. Many benthic marine invertebrates, including sponges and bryozoans, contain natural products with antimicrobial properties, since microbes usually constitute the first stages of fouling. Extracts from 4 Antarctic sponges (Myxilla (Myxilla) mollis, Mycale tylotornota, Rossella nuda, and Anoxycalyx (Scolymastra) joubini) and 2 bryozoan species (Cornucopina pectogemma and Nematoflustra flagellata) were tested separately for antifouling properties in field experiments. The different crude extracts from these invertebrates were incorporated into a substratum gel at natural concentrations for an ecological approach. Treatments were tested by submerging plates covered by these substratum gels under water in situ during 1 lunar cycle (28 d) at Deception Island (South Shetland Islands, Antarctica). Remarkably, the butanolic extracts of M. tylotornota and C. pectogemma showed complete growth inhibition of microscopic eukaryotic organisms, one of the succession stages involved in biofouling. Our results suggest that different chemical strategies may exist to avoid fouling, although the role of chemical defenses is often species-specific. Thus, the high specificity of the microbial community attached to the coated plates seems to be modulated by the chemical cues of the crude extracts of the invertebrates tested.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"98 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85779761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Growth and phosphatase activities of Ostreopsis cf. ovata biofilms supplied with diverse dissolved organic phosphorus (DOP) compounds 提供不同溶解有机磷(DOP)化合物的卵形Ostreopsis cf. ovata生物膜的生长和磷酸酶活性

IF 1.4 4区环境科学与生态学

Aquatic Microbial Ecology Pub Date : 2020-11-05 DOI: 10.3354/ame01946

N. Ellwood, M. Pasella, C. Totti, S. Accoroni

{"title":"Growth and phosphatase activities of Ostreopsis cf. ovata biofilms supplied with diverse dissolved organic phosphorus (DOP) compounds","authors":"N. Ellwood, M. Pasella, C. Totti, S. Accoroni","doi":"10.3354/ame01946","DOIUrl":"https://doi.org/10.3354/ame01946","url":null,"abstract":"It is becoming increasingly evident that the use of organic nutrients is widespread among many aquatic phototrophic organisms. Simultaneously, incidents of eutrophication of coastal waters are becoming more common due to rises in organic nutrient loads deriving from anthropogenic activities and natural terrestrial processes. In the northern Adriatic Sea, blooms of the toxic dinoflagellate Ostreopsis cf. ovata are reported as a frequent phenomenon linked to particular environmental conditions, including increased organic nutrient loads. Ostreopsis blooms typically produce a mucilaginous biofilm that can cover all benthic substrata. In order to clarify the role of dissolved organic phosphorus (DOP) in the onset and maintenance of an O. cf. ovata bloom, we investigated the growth rates in the presence of a range of phosphomonoesters (PMEs) (D-fructose 1,6-disphosphate, β-glycerophosphate, α-D-glucose 1-phosphate, guanosine 5’-monophosphate and phytic acid) and phosphodiesters (PDEs) (DNA and RNA). Levels of both phosphomonoesterase (PMEase) and phosphodiesterase (PDEase) activities were assessed in the O. cf. ovata biofilms. The results showed that O. cf. ovata growth is not inhibited in media containing a wide range of DOP and diverse ratios of PME:PDE compared to those containing inorganic phosphorus. Much of the hydrolytic activity was associated with bacteria and with extracellular polymeric substances (EPSs). Our findings suggest that the success of O. cf. ovata stems from the collective participation of all components of the biofilm (O. cf. ovata, EPSs and bacteria) that allows it to thrive in phosphorus-limited environments, but where the main source of phosphorus is organic.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"35 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2020-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72989878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Novel method for measuring aquatic bacterial productivity using D10-leucine based on protein synthesis rate 基于蛋白质合成速率的d10 -亮氨酸测定水生细菌产量的新方法

IF 1.4 4区环境科学与生态学

Aquatic Microbial Ecology Pub Date : 2020-10-22 DOI: 10.3354/ame01945

K. Tsuchiya, T. Sano, N. Tomioka, K. Kazuhiro, A. Imai, K. Hayakawa, Takamaru Nagata, T. Okamoto, V. Kuwahara, A. Kohzu

{"title":"Novel method for measuring aquatic bacterial productivity using D10-leucine based on protein synthesis rate","authors":"K. Tsuchiya, T. Sano, N. Tomioka, K. Kazuhiro, A. Imai, K. Hayakawa, Takamaru Nagata, T. Okamoto, V. Kuwahara, A. Kohzu","doi":"10.3354/ame01945","DOIUrl":"https://doi.org/10.3354/ame01945","url":null,"abstract":"The most widely used method for measuring bacterial production is tritium-labeled leucine (3H-Leu). Although this method provides methodological simplicity and high sensitivity, the employment of radioactive isotopes is often restricted by regulations, particularly in field settings. In this study, we developed a non-radioactive method for measuring bacterial productivity based on the protein synthesis rate, using deuterium-labeled leucine ((CD3)2CDCD2CD(NH2)COOH; D10-Leu); the proposed method was then compared and verified with the3H-Leu method. The procedures of the proposed method are (1) incorporation of D10-Leu by bacteria, (2) acid hydrolysis (HCl) to amino acids and (3) quantification of D10-Leu (m/z142.10) by liquid chromatography mass spectrometry (LC-MS/MS). In the LC-MS/MS analysis, we detected a larger amount of D9-Leu (m/z141.10) and D8-Leu (m/z140.10) than that of D10-Leu, suggesting that incorporated D10-Leu was rapidly metabolized such as in deamination and aminotransferase reactions. The incorporation rates of D10-Leu, D10-Leu + D9-Leu (D10+D9-Leu) and D10-Leu + D9-Leu + D8-Leu (D10+D9+D8-Leu) were significantly positively correlated to that of3H-Leu, confirming the validity of the proposed method. Since D7-Leu (m/z139.10) could not be detected, the amount of exogenous leucine incorporated into protein can be accurately estimated through D10+D9+D8-Leu measurement. The new compound-based quantification method using stable isotope-labeled leucine can be a powerful tool to estimate pure protein synthesis rate for measuring bacterial production.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"28 1","pages":"121-129"},"PeriodicalIF":1.4,"publicationDate":"2020-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87307258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contribution of large bacteria to bacterial biomass in a deep freshwater lake (Lake Biwa, Japan) 深层淡水湖(日本琵琶湖)大型细菌对细菌生物量的贡献

IF 1.4 4区环境科学与生态学

Aquatic Microbial Ecology Pub Date : 2020-10-22 DOI: 10.3354/ame01949

S. Shen, Y. Shimizu

{"title":"Contribution of large bacteria to bacterial biomass in a deep freshwater lake (Lake Biwa, Japan)","authors":"S. Shen, Y. Shimizu","doi":"10.3354/ame01949","DOIUrl":"https://doi.org/10.3354/ame01949","url":null,"abstract":"Despite the importance of bacterial cell volume in microbial ecology in aquatic environments, literature regarding the effects of seasonal and spatial variations on bacterial cell volume remains scarce. We used transmission electron microscopy to examine seasonal and spatial variations in bacterial cell size for 18 mo in 2 layers (epilimnion 0.5 m and hypolimnion 60 m) of Lake Biwa, Japan, a large and deep freshwater lake. During the stratified period, we found that the bacterial cell volume in the hypolimnion ranged from 0.017 to 0.12 µm3 (median), whereas that in the epilimnion was less variable (0.016 to 0.033 µm3, median) and much lower than that in the hypolimnion. Additionally, in the hypolimnion, cell volume during the stratified period was greater than that during the mixing period (up to 5.7-fold). These differences in cell volume resulted in comparable bacterial biomass in the hypolimnion and epilimnion, despite the fact that there was lower bacterial abundance in the hypolimnion than in the epilimnion. We also found that the biomass of larger bacteria, which are not likely to be grazed by heterotrophic nanoflagellates, increased in the hypolimnion during the stratified period. Our data suggest that estimation of carbon flux (e.g. bacterial productivity) needs to be interpreted cautiously when cell volume is used as a constant parametric value. In deep freshwater lakes, a difference in cell volume with seasonal and spatial variation may largely affect estimations.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"10 1","pages":"131-139"},"PeriodicalIF":1.4,"publicationDate":"2020-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89130584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Uncovering cryptochrome/photolyase gene diversity in aquatic microbiomes exposed to diverse UV-B regimes 揭示暴露于不同UV-B环境下水生微生物群的隐色素/光解酶基因多样性

IF 1.4 4区环境科学与生态学

Aquatic Microbial Ecology Pub Date : 2020-10-22 DOI: 10.3354/ame01947

D. G. Alonso-Reyes, M. E. Farías, V. Albarracín

{"title":"Uncovering cryptochrome/photolyase gene diversity in aquatic microbiomes exposed to diverse UV-B regimes","authors":"D. G. Alonso-Reyes, M. E. Farías, V. Albarracín","doi":"10.3354/ame01947","DOIUrl":"https://doi.org/10.3354/ame01947","url":null,"abstract":"During evolution, microorganisms exposed to high amounts of UV-B irradiation developed fine-tuned photo-enzymes called ‘photolyases’ to cope with DNA damage caused by UV-B. These photoreceptors, belonging to the cryptochrome/photolyase family (CPF), have been well characterized at the genomic and proteomic level in bacteria isolated from a wide range of environments. In this work, we go further towards studying the abundance of CPF in aquatic microbial communities from different geographic regions across the globe. Metagenomics data combined with geo-referenced solar irradiation measurements indicated that the higher the UV-B level in the microbiome’s environment, the higher the abundance of CPF genes and lower the microbial diversity. A connection between CPF abundance and radiation intensity/photoperiod was found. Likewise, cryptochrome-like genes were found to be abundant in most exposed microbiomes, indicating a complementary role to standard photolyases. We observed that CPFs are more likely to be present in dominant taxa of the highly irradiated microbiomes, suggesting an evolutionary force for survival and dominance under extreme solar exposure. This work reports 3 novel CPF clades, proving the potential of global metagenomic analyses in detecting novel proteins.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"370 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2020-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77971050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effects of polyphenols on plankton assemblages and bacterial abundance representative of a pampean shallow lake: an experimental study 多酚对潘潘浅水湖泊浮游生物群落和细菌丰度影响的实验研究

IF 1.4 4区环境科学与生态学

Aquatic Microbial Ecology Pub Date : 2020-10-08 DOI: 10.3354/ame01943

M. Alvarez, H. Benítez, L. Solari, Jordi Cortés, N. Gabellone, M. Claps

{"title":"Effects of polyphenols on plankton assemblages and bacterial abundance representative of a pampean shallow lake: an experimental study","authors":"M. Alvarez, H. Benítez, L. Solari, Jordi Cortés, N. Gabellone, M. Claps","doi":"10.3354/ame01943","DOIUrl":"https://doi.org/10.3354/ame01943","url":null,"abstract":"Shallow lakes are complex environments that play an important role in ecology. Their relationships with the surrounding areas make these lentic water bodies susceptible to alterations in response to human activity. For example, the input of polyphenols could be critical in altering species interactions. The shallow lakes located in the pampean plain of Argentina are major reservoirs of diversity, with the environmental habitats of the Salado River basin in particular having become impacted by human activities. The aim of this work was to evaluate the effects of different polyphenol concentrations (i.e. tannins) on changes in the plankton assemblages and on the abundance of bacteria in San Miguel del Monte Lake. Factorial experiments under laboratory conditions (4 tannin concentration levels and 4 response times) revealed that high tannin concentrations affected neither the phyto- and zooplankton assemblages nor bacterial abundances. Major changes both in assemblages and in the bacterioplankton abundances were dependent on exposure time. We found that the initial food web of grazers shifted to detritivorous feeding, thus producing a simplification of the community toward early succession. Although no effects of polyphenols were registered here, what proved striking was the resilience of the system and the capability to support high tannin concentrations throughout the experiment. Therefore, future investigations are necessary to elucidate the key role of such humic substances in the structuring of plankton assemblages of shallow lakes from strongly impacted areas, such as those found in the pampean region.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"36 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2020-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79881672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial and nutrient dynamics in mangrove, reef, and seagrass waters over tidal and diurnal time scales 红树林、珊瑚礁和海草水域在潮汐和昼夜时间尺度上的微生物和营养动态

IF 1.4 4区环境科学与生态学

Aquatic Microbial Ecology Pub Date : 2020-10-08 DOI: 10.3354/ame01944

C. Becker, L. Weber, J. Suca, J. Llopiz, T. Mooney, A. Apprill

{"title":"Microbial and nutrient dynamics in mangrove, reef, and seagrass waters over tidal and diurnal time scales","authors":"C. Becker, L. Weber, J. Suca, J. Llopiz, T. Mooney, A. Apprill","doi":"10.3354/ame01944","DOIUrl":"https://doi.org/10.3354/ame01944","url":null,"abstract":"In coral reefs and adjacent seagrass meadow and mangrove environments, short temporal scales (i.e. tidal, diurnal) may have important influences on ecosystem processes and community structure, but these scales are rarely investigated. This study examines how tidal and diurnal forcings influence pelagic microorganisms and nutrient dynamics in 3 important and adjacent coastal biomes: mangroves, coral reefs, and seagrass meadows. We sampled for microbial (Bacteria and Archaea) community composition, cell abundances and environmental parameters at 9 coastal sites on St. John, US Virgin Islands that spanned 4 km in distance (4 coral reefs, 2 seagrass meadows and 3 mangrove locations within 2 larger bays). Eight samplings occurred over a 48 h period, capturing day and night microbial dynamics over 2 tidal cycles. The seagrass and reef biomes ex hibited relatively consistent environmental conditions and microbial community structure but were dominated by shifts in picocyanobacterial abundances that were most likely attributed to diel dynamics. In contrast, mangrove ecosystems exhibited substantial daily shifts in environmental parameters, heterotrophic cell abundances and microbial community structure that were consistent with the tidal cycle. Differential abundance analysis of mangrove-associated microorganisms revealed enrichment of pelagic oligotrophic taxa during high tide and enrichment of putative sediment-associated microbes during low tide. Our study underpins the importance of tidal and diurnal time scales in structuring coastal microbial and nutrient dynamics, with diel and tidal cycles contributing to a highly dynamic microbial environment in mangroves, and time of day likely contributing to microbial dynamics in seagrass and reef biomes.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"31 1","pages":"101-119"},"PeriodicalIF":1.4,"publicationDate":"2020-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86633444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Unearthing the sand microbiome of sea turtle nests with disparate survivorship at a mass-nesting beach in Costa Rica 在哥斯达黎加的一个大量筑巢的海滩上，发现了不同存活率的海龟巢的沙子微生物群

IF 1.4 4区环境科学与生态学

Aquatic Microbial Ecology Pub Date : 2020-09-03 DOI: 10.3354/ame01941

Vanessa S. Bézy, K. Hill-Spanik, C. Plante

{"title":"Unearthing the sand microbiome of sea turtle nests with disparate survivorship at a mass-nesting beach in Costa Rica","authors":"Vanessa S. Bézy, K. Hill-Spanik, C. Plante","doi":"10.3354/ame01941","DOIUrl":"https://doi.org/10.3354/ame01941","url":null,"abstract":"For endangered sea turtle populations, microbial pathogens of developing embryos are of concern at nesting sites around the globe. For olive ridley turtles, hatching success is markedly lower at mass-nesting sites than at solitary nesting beaches, a case presumably resulting from the abundance of decomposing eggs generated by nesting turtles destroying adjacent eggs. This organic input drives microbial activity, affecting the nest environment (i.e. pO2 and temperature), and reducing embryo survivorship and hatching success. However, the composition of microbial communities in nest sand has not been studied in detail and the presence of potential pathogens can, therefore, not be discounted. As a part of a larger study that investigated microbial abundance in nests, we employed high-throughput DNA sequencing to compare fungal and bacterial composition in nest sand from areas of disparate embryo survivorship. While we found no differences in alpha-diversity (mean operational taxonomic unit diversity within each site) among nesting areas, the microbial community composition of each area was distinct, and differences in community structure corresponded with variable hatching success. Some sequences of potential sea turtle egg pathogens were obtained (e.g. Fusarium solani species complex), but were in low relative abundance, and their presence was not associated with low hatching success. Our results from the arribada beach at Ostional, Costa Rica, provide further evidence that the physical characteristics of the nest (including those that determine microbial composition and activity) are likely more relevant to hatching success than the presence of potential pathogens or microbial community structure alone.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"850 1","pages":"71-83"},"PeriodicalIF":1.4,"publicationDate":"2020-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84746235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Ammonium loading drives bacterial community shifts in biofilms attached to the submerged macrophyte Hydrilla verticillata 铵负载驱动附着在沉水大型植物水螅的生物膜中的细菌群落转移

IF 1.4 4区环境科学与生态学

Aquatic Microbial Ecology Pub Date : 2020-08-20 DOI: 10.3354/ame01942

Da Lin, Liang Zhu, C. Qiu, Xiaoying Mu, Songhe Zhang, O. E. Ohore

{"title":"Ammonium loading drives bacterial community shifts in biofilms attached to the submerged macrophyte Hydrilla verticillata","authors":"Da Lin, Liang Zhu, C. Qiu, Xiaoying Mu, Songhe Zhang, O. E. Ohore","doi":"10.3354/ame01942","DOIUrl":"https://doi.org/10.3354/ame01942","url":null,"abstract":"The effects of ammonium on biofilm attached to submerged macrophytes remain unclear. We studied the microbial communities of epiphytic biofilms on the leaf surface of Hydrilla verticillata under increasing ammonium loadings using 16S rRNA gene sequencing and RT-PCR. The study showed that the microbial community structures were influenced by increased ammonium loadings. N-cycling bacteria including the nitrifier Nitrospira and the denitrifiers Rhodobacter, Hyphomicrobium, and Flavobacterium were found to be predominant. Copy numbers of Ncycling functional genes (including amoA, nxrA, narG, napA, nirK, nirS, qnorB, and nosZ) were stimulated by increased ammonium loadings, showing the high potential of attached biofilms in removing ammonium-N through coupled nitrification-denitrification. Our results indicated that submerged macrophytes facilitated the ammonium removal process not only by providing favorable surface niches for ammonium oxidizers, nitrifiers, and denitrifiers in the attached biofilms but also by mediating dissolved oxygen concentrations and alkalinity condition in the water.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"17 3 1","pages":"59-69"},"PeriodicalIF":1.4,"publicationDate":"2020-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77390427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4