Ammonium loading drives bacterial community shifts in biofilms attached to the submerged macrophyte Hydrilla verticillata

IF 1.6 4区环境科学与生态学 Q3 ECOLOGY

Aquatic Microbial Ecology Pub Date : 2020-08-20 DOI:10.3354/ame01942

Da Lin, Liang Zhu, C. Qiu, Xiaoying Mu, Songhe Zhang, O. E. Ohore

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引用次数: 4

Abstract

The effects of ammonium on biofilm attached to submerged macrophytes remain unclear. We studied the microbial communities of epiphytic biofilms on the leaf surface of Hydrilla verticillata under increasing ammonium loadings using 16S rRNA gene sequencing and RT-PCR. The study showed that the microbial community structures were influenced by increased ammonium loadings. N-cycling bacteria including the nitrifier Nitrospira and the denitrifiers Rhodobacter, Hyphomicrobium, and Flavobacterium were found to be predominant. Copy numbers of Ncycling functional genes (including amoA, nxrA, narG, napA, nirK, nirS, qnorB, and nosZ) were stimulated by increased ammonium loadings, showing the high potential of attached biofilms in removing ammonium-N through coupled nitrification-denitrification. Our results indicated that submerged macrophytes facilitated the ammonium removal process not only by providing favorable surface niches for ammonium oxidizers, nitrifiers, and denitrifiers in the attached biofilms but also by mediating dissolved oxygen concentrations and alkalinity condition in the water.

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铵负载驱动附着在沉水大型植物水螅的生物膜中的细菌群落转移

铵对沉水植物附着生物膜的影响尚不清楚。采用16S rRNA基因测序和RT-PCR技术，研究了增加铵负荷下水螅叶表面附生生物膜的微生物群落。研究表明，微生物群落结构受铵负荷增加的影响。氮循环细菌包括硝化菌硝化螺旋菌和反硝化菌红杆菌、菌丝微生物和黄杆菌。氮循环功能基因(包括amoA, nxrA, narG, napA, nirK, nirS, qnorB和nosZ)的拷贝数随着铵负荷的增加而增加，表明附着生物膜通过耦合硝化-反硝化去除氨氮的潜力很大。研究结果表明，沉水植物不仅通过在附着的生物膜上为氨氧化菌、硝化菌和反硝化菌提供有利的表面生态位，而且通过调节水体溶解氧浓度和碱度条件，促进了氨的去除过程。

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来源期刊

Aquatic Microbial Ecology 环境科学-海洋与淡水生物学

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

3.0 months

期刊介绍： AME is international and interdisciplinary. It presents rigorously refereed and carefully selected Research Articles, Reviews and Notes, as well as Comments/Reply Comments (for details see AME 27:209), Opinion Pieces (previously called ''As I See It'') and AME Specials. For details consult the Guidelines for Authors. Papers may be concerned with: Tolerances and responses of microorganisms to variations in abiotic and biotic components of their environment; microbial life under extreme environmental conditions (climate, temperature, pressure, osmolarity, redox, etc.). Role of aquatic microorganisms in the production, transformation and decomposition of organic matter; flow patterns of energy and matter as these pass through microorganisms; population dynamics; trophic interrelationships; modelling, both theoretical and via computer simulation, of individual microorganisms and microbial populations; biodiversity. Absorption and transformation of inorganic material; synthesis and transformation of organic material (autotrophic and heterotrophic); non-genetic and genetic adaptation; behaviour; molecular microbial ecology; symbioses.