Dalton E Carter, Tao Peng, Melanie R Moody, Shao-Ling Huang, David D McPherson, Melvin E Klegerman
{"title":"An Echogenic Clot Method for Thrombolysis Monitoring in Thrombotic Stroke Models.","authors":"Dalton E Carter, Tao Peng, Melanie R Moody, Shao-Ling Huang, David D McPherson, Melvin E Klegerman","doi":"10.18103/mra.v11i3.3702","DOIUrl":"10.18103/mra.v11i3.3702","url":null,"abstract":"<p><p>To demonstrate thrombolytic efficacy of a tissue plasminogen activator (tPA)-loaded echogenic liposome (TELIP) formulation in a rabbit thrombotic stroke model (the most relevant animal model for evaluation of directed thrombolytic therapy for ischemic stroke), we sought to develop a means of monitoring thrombus dissolution quantitatively by ultrasound imaging methods. We hypothesized that a gas-free ultrasound contrast agent can be incorporated into blood clots at a concentration that does not affect the tPA-mediated clot dissolution rate, while enabling quantitative assessment of the clot dissolution rate. Clots were formed from a mixture of whole rabbit blood, 1 M calcium chloride, human thrombin and varying amounts of microcrystalline cellulose. Washed clots in tubes were weighed at 30, 60 and 90 minutes after addition of recombinant tPA (rtPA) in porcine plasma (100 μg/ml). Clot echogenicity at each time point was assessed using a Philips HDI 5000 ultrasound system using an L12-5 linear array probe. Recorded Images underwent videodensitometric analysis that converted image reflectivity to mean gray scale values (MGSV). We found that 1.12 mg/ml of microcrystalline cellulose in rabbit blood clots (0.2 ml) provided optimal echogenicity without affecting clot dissolution rates (0.3-0.6 mg/min.) caused by rtPA. The clot dissolution rate measured by videodensitometric analysis of the echogenic clots agreed well with that determined by mass loss measurements (0.28% 0-time value/minute). This method will be important for demonstrating <i>in vivo</i> efficacy with potentially decreased hemorrhagic effects provided by directed tPA vehicles relative to systemic administration of the free thrombolytic.</p>","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73435149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antonija Kulaš, P. Žutinić, Vesna Gulin Beljak, Renata Matoničkin Kepčija, M. Perić, S. Orlić, I. Petrić, T. Marković, M. Gligora Udovič
{"title":"Diversity of protist genera in periphyton of tufa-depositing karstic river","authors":"Antonija Kulaš, P. Žutinić, Vesna Gulin Beljak, Renata Matoničkin Kepčija, M. Perić, S. Orlić, I. Petrić, T. Marković, M. Gligora Udovič","doi":"10.1186/s13213-023-01712-z","DOIUrl":"https://doi.org/10.1186/s13213-023-01712-z","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":" ","pages":"1-13"},"PeriodicalIF":3.0,"publicationDate":"2023-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48804588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Structural diversity of bacterial communities in two divergent sunflower rhizosphere soils","authors":"B. Nwachukwu, A. Ayangbenro, O. O. Babalola","doi":"10.1186/s13213-023-01713-y","DOIUrl":"https://doi.org/10.1186/s13213-023-01713-y","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":"73 1","pages":"1-18"},"PeriodicalIF":3.0,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44561670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Warming changes the composition and diversity of fungal communities in permafrost","authors":"Y. Jiang, Libin Yang, Song Wu, Tian Zhou","doi":"10.1186/s13213-022-01707-2","DOIUrl":"https://doi.org/10.1186/s13213-022-01707-2","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":" ","pages":"1-12"},"PeriodicalIF":3.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48233153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiao-Jiao Pan, Yeqing Han, Cen Wang, Jing-nan Du, Yu Wang, Yue Chen, Xinduo Huang, Kaixuan Ma, Zhiguo Zhang, Naisheng Li
{"title":"Analysis of microbial community and biodeterioration of maritime cultural relics (ironware, porcelain, axes, hull wood) from the Nanhai No. 1 shipwreck","authors":"Jiao-Jiao Pan, Yeqing Han, Cen Wang, Jing-nan Du, Yu Wang, Yue Chen, Xinduo Huang, Kaixuan Ma, Zhiguo Zhang, Naisheng Li","doi":"10.1186/s13213-022-01705-4","DOIUrl":"https://doi.org/10.1186/s13213-022-01705-4","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":"73 1","pages":"1-12"},"PeriodicalIF":3.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42240621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and screening of low-density polyethylene (LDPE) bags degrading bacteria from Addis Ababa municipal solid waste disposal site “Koshe”","authors":"Zuriash Mamo Nademo, N. T. Shibeshi, M. T. Gemeda","doi":"10.1186/s13213-023-01711-0","DOIUrl":"https://doi.org/10.1186/s13213-023-01711-0","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":" ","pages":"1-11"},"PeriodicalIF":3.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47900939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"regO: a novel locus in the regulation of tetrapyrrole biosynthesis in Rhodospirillum rubrum","authors":"M. Mansour, K. Abou-Aisha","doi":"10.1186/s13213-022-01709-0","DOIUrl":"https://doi.org/10.1186/s13213-022-01709-0","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":" ","pages":"1-12"},"PeriodicalIF":3.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46336137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tianming Hu, Jiating Wei, Li-jun Du, Jibao Chen, Jun Zhang
{"title":"The effect of biochar on nitrogen availability and bacterial community in farmland","authors":"Tianming Hu, Jiating Wei, Li-jun Du, Jibao Chen, Jun Zhang","doi":"10.1186/s13213-022-01708-1","DOIUrl":"https://doi.org/10.1186/s13213-022-01708-1","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":"73 1","pages":"1-11"},"PeriodicalIF":3.0,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44905953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jingjing Cui, Mengmeng Liu, Weiwei Wu, C. Long, B. Zeng
{"title":"The acyl-CoA-binding protein 2 exhibited the highest affinity for palmitoyl-CoA and promoted Monascus pigment production","authors":"Jingjing Cui, Mengmeng Liu, Weiwei Wu, C. Long, B. Zeng","doi":"10.1186/s13213-023-01710-1","DOIUrl":"https://doi.org/10.1186/s13213-023-01710-1","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":"73 1","pages":"1-7"},"PeriodicalIF":3.0,"publicationDate":"2023-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47138743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. M. Prosdocimi, S. Arioli, F. Mapelli, Z. Zeaiter, M. Fusi, D. Daffonchio, S. Borin, E. Crotti
{"title":"Cell phenotype changes and oxidative stress response in Vibrio spp. induced into viable but non-culturable (VBNC) state","authors":"E. M. Prosdocimi, S. Arioli, F. Mapelli, Z. Zeaiter, M. Fusi, D. Daffonchio, S. Borin, E. Crotti","doi":"10.1186/s13213-022-01703-6","DOIUrl":"https://doi.org/10.1186/s13213-022-01703-6","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":"73 1","pages":"1-13"},"PeriodicalIF":3.0,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43562126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}