Annals of Microbiology最新文献

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An Echogenic Clot Method for Thrombolysis Monitoring in Thrombotic Stroke Models. 血栓性脑卒中模型中用于溶栓监测的回声凝块方法。
4区 生物学
Annals of Microbiology Pub Date : 2023-03-01 DOI: 10.18103/mra.v11i3.3702
Dalton E Carter, Tao Peng, Melanie R Moody, Shao-Ling Huang, David D McPherson, Melvin E Klegerman
{"title":"An Echogenic Clot Method for Thrombolysis Monitoring in Thrombotic Stroke Models.","authors":"Dalton E Carter, Tao Peng, Melanie R Moody, Shao-Ling Huang, David D McPherson, Melvin E Klegerman","doi":"10.18103/mra.v11i3.3702","DOIUrl":"10.18103/mra.v11i3.3702","url":null,"abstract":"<p><p>To demonstrate thrombolytic efficacy of a tissue plasminogen activator (tPA)-loaded echogenic liposome (TELIP) formulation in a rabbit thrombotic stroke model (the most relevant animal model for evaluation of directed thrombolytic therapy for ischemic stroke), we sought to develop a means of monitoring thrombus dissolution quantitatively by ultrasound imaging methods. We hypothesized that a gas-free ultrasound contrast agent can be incorporated into blood clots at a concentration that does not affect the tPA-mediated clot dissolution rate, while enabling quantitative assessment of the clot dissolution rate. Clots were formed from a mixture of whole rabbit blood, 1 M calcium chloride, human thrombin and varying amounts of microcrystalline cellulose. Washed clots in tubes were weighed at 30, 60 and 90 minutes after addition of recombinant tPA (rtPA) in porcine plasma (100 μg/ml). Clot echogenicity at each time point was assessed using a Philips HDI 5000 ultrasound system using an L12-5 linear array probe. Recorded Images underwent videodensitometric analysis that converted image reflectivity to mean gray scale values (MGSV). We found that 1.12 mg/ml of microcrystalline cellulose in rabbit blood clots (0.2 ml) provided optimal echogenicity without affecting clot dissolution rates (0.3-0.6 mg/min.) caused by rtPA. The clot dissolution rate measured by videodensitometric analysis of the echogenic clots agreed well with that determined by mass loss measurements (0.28% 0-time value/minute). This method will be important for demonstrating <i>in vivo</i> efficacy with potentially decreased hemorrhagic effects provided by directed tPA vehicles relative to systemic administration of the free thrombolytic.</p>","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73435149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diversity of protist genera in periphyton of tufa-depositing karstic river 凝灰岩沉积岩溶河流外围生物属的多样性
IF 3 4区 生物学
Annals of Microbiology Pub Date : 2023-02-18 DOI: 10.1186/s13213-023-01712-z
Antonija Kulaš, P. Žutinić, Vesna Gulin Beljak, Renata Matoničkin Kepčija, M. Perić, S. Orlić, I. Petrić, T. Marković, M. Gligora Udovič
{"title":"Diversity of protist genera in periphyton of tufa-depositing karstic river","authors":"Antonija Kulaš, P. Žutinić, Vesna Gulin Beljak, Renata Matoničkin Kepčija, M. Perić, S. Orlić, I. Petrić, T. Marković, M. Gligora Udovič","doi":"10.1186/s13213-023-01712-z","DOIUrl":"https://doi.org/10.1186/s13213-023-01712-z","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":" ","pages":"1-13"},"PeriodicalIF":3.0,"publicationDate":"2023-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48804588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural diversity of bacterial communities in two divergent sunflower rhizosphere soils 两种不同向日葵根际土壤细菌群落结构多样性
IF 3 4区 生物学
Annals of Microbiology Pub Date : 2023-02-17 DOI: 10.1186/s13213-023-01713-y
B. Nwachukwu, A. Ayangbenro, O. O. Babalola
{"title":"Structural diversity of bacterial communities in two divergent sunflower rhizosphere soils","authors":"B. Nwachukwu, A. Ayangbenro, O. O. Babalola","doi":"10.1186/s13213-023-01713-y","DOIUrl":"https://doi.org/10.1186/s13213-023-01713-y","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":"73 1","pages":"1-18"},"PeriodicalIF":3.0,"publicationDate":"2023-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44561670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Warming changes the composition and diversity of fungal communities in permafrost 变暖改变了永久冻土真菌群落的组成和多样性
IF 3 4区 生物学
Annals of Microbiology Pub Date : 2023-02-03 DOI: 10.1186/s13213-022-01707-2
Y. Jiang, Libin Yang, Song Wu, Tian Zhou
{"title":"Warming changes the composition and diversity of fungal communities in permafrost","authors":"Y. Jiang, Libin Yang, Song Wu, Tian Zhou","doi":"10.1186/s13213-022-01707-2","DOIUrl":"https://doi.org/10.1186/s13213-022-01707-2","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":" ","pages":"1-12"},"PeriodicalIF":3.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48233153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Analysis of microbial community and biodeterioration of maritime cultural relics (ironware, porcelain, axes, hull wood) from the Nanhai No. 1 shipwreck 南海一号沉船海洋文物(铁器、瓷器、斧头、船体木材)微生物群落及生物降解分析
IF 3 4区 生物学
Annals of Microbiology Pub Date : 2023-02-03 DOI: 10.1186/s13213-022-01705-4
Jiao-Jiao Pan, Yeqing Han, Cen Wang, Jing-nan Du, Yu Wang, Yue Chen, Xinduo Huang, Kaixuan Ma, Zhiguo Zhang, Naisheng Li
{"title":"Analysis of microbial community and biodeterioration of maritime cultural relics (ironware, porcelain, axes, hull wood) from the Nanhai No. 1 shipwreck","authors":"Jiao-Jiao Pan, Yeqing Han, Cen Wang, Jing-nan Du, Yu Wang, Yue Chen, Xinduo Huang, Kaixuan Ma, Zhiguo Zhang, Naisheng Li","doi":"10.1186/s13213-022-01705-4","DOIUrl":"https://doi.org/10.1186/s13213-022-01705-4","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":"73 1","pages":"1-12"},"PeriodicalIF":3.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42240621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and screening of low-density polyethylene (LDPE) bags degrading bacteria from Addis Ababa municipal solid waste disposal site “Koshe” 亚的斯亚贝巴城市固体废物处理场" Koshe "低密度聚乙烯(LDPE)袋降解细菌的分离和筛选
IF 3 4区 生物学
Annals of Microbiology Pub Date : 2023-01-19 DOI: 10.1186/s13213-023-01711-0
Zuriash Mamo Nademo, N. T. Shibeshi, M. T. Gemeda
{"title":"Isolation and screening of low-density polyethylene (LDPE) bags degrading bacteria from Addis Ababa municipal solid waste disposal site “Koshe”","authors":"Zuriash Mamo Nademo, N. T. Shibeshi, M. T. Gemeda","doi":"10.1186/s13213-023-01711-0","DOIUrl":"https://doi.org/10.1186/s13213-023-01711-0","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":" ","pages":"1-11"},"PeriodicalIF":3.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47900939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
regO: a novel locus in the regulation of tetrapyrrole biosynthesis in Rhodospirillum rubrum regO:红红螺旋菌四吡咯生物合成调控的新位点
IF 3 4区 生物学
Annals of Microbiology Pub Date : 2023-01-19 DOI: 10.1186/s13213-022-01709-0
M. Mansour, K. Abou-Aisha
{"title":"regO: a novel locus in the regulation of tetrapyrrole biosynthesis in Rhodospirillum rubrum","authors":"M. Mansour, K. Abou-Aisha","doi":"10.1186/s13213-022-01709-0","DOIUrl":"https://doi.org/10.1186/s13213-022-01709-0","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":" ","pages":"1-12"},"PeriodicalIF":3.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46336137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of biochar on nitrogen availability and bacterial community in farmland 生物炭对农田氮素有效性和细菌群落的影响
IF 3 4区 生物学
Annals of Microbiology Pub Date : 2023-01-11 DOI: 10.1186/s13213-022-01708-1
Tianming Hu, Jiating Wei, Li-jun Du, Jibao Chen, Jun Zhang
{"title":"The effect of biochar on nitrogen availability and bacterial community in farmland","authors":"Tianming Hu, Jiating Wei, Li-jun Du, Jibao Chen, Jun Zhang","doi":"10.1186/s13213-022-01708-1","DOIUrl":"https://doi.org/10.1186/s13213-022-01708-1","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":"73 1","pages":"1-11"},"PeriodicalIF":3.0,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44905953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
The acyl-CoA-binding protein 2 exhibited the highest affinity for palmitoyl-CoA and promoted Monascus pigment production 酰基辅酶A结合蛋白2对棕榈酰基辅酶A表现出最高的亲和力,并促进红曲霉色素的产生
IF 3 4区 生物学
Annals of Microbiology Pub Date : 2023-01-07 DOI: 10.1186/s13213-023-01710-1
Jingjing Cui, Mengmeng Liu, Weiwei Wu, C. Long, B. Zeng
{"title":"The acyl-CoA-binding protein 2 exhibited the highest affinity for palmitoyl-CoA and promoted Monascus pigment production","authors":"Jingjing Cui, Mengmeng Liu, Weiwei Wu, C. Long, B. Zeng","doi":"10.1186/s13213-023-01710-1","DOIUrl":"https://doi.org/10.1186/s13213-023-01710-1","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":"73 1","pages":"1-7"},"PeriodicalIF":3.0,"publicationDate":"2023-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47138743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell phenotype changes and oxidative stress response in Vibrio spp. induced into viable but non-culturable (VBNC) state 弧菌的细胞表型变化和氧化应激反应。诱导进入可存活但不可培养(VBNC)状态
IF 3 4区 生物学
Annals of Microbiology Pub Date : 2023-01-04 DOI: 10.1186/s13213-022-01703-6
E. M. Prosdocimi, S. Arioli, F. Mapelli, Z. Zeaiter, M. Fusi, D. Daffonchio, S. Borin, E. Crotti
{"title":"Cell phenotype changes and oxidative stress response in Vibrio spp. induced into viable but non-culturable (VBNC) state","authors":"E. M. Prosdocimi, S. Arioli, F. Mapelli, Z. Zeaiter, M. Fusi, D. Daffonchio, S. Borin, E. Crotti","doi":"10.1186/s13213-022-01703-6","DOIUrl":"https://doi.org/10.1186/s13213-022-01703-6","url":null,"abstract":"","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":"73 1","pages":"1-13"},"PeriodicalIF":3.0,"publicationDate":"2023-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43562126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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