Annals of Microbiology最新文献_第10页

Spatial differences in Casuarina equisetifolia L. endophyte community structure 木麻黄内生菌群落结构的空间差异

IF 3 4区生物学

Annals of Microbiology Pub Date : 2022-07-28 DOI: 10.1186/s13213-022-01685-5

Lin, Qi, Xu, Zhixia, Li, Miaomiao, Wang, Ying, Li, Lei

{"title":"Spatial differences in Casuarina equisetifolia L. endophyte community structure","authors":"Lin, Qi, Xu, Zhixia, Li, Miaomiao, Wang, Ying, Li, Lei","doi":"10.1186/s13213-022-01685-5","DOIUrl":"https://doi.org/10.1186/s13213-022-01685-5","url":null,"abstract":"Casuarina equisetifolia, a fast-growing, abundant tree species on the southeastern coast of China, plays an important role in protecting the coastal environment, but the ecological processes that govern microbiome assembly and within-plant microorganism transmission are poorly known. In this paper, we used ITS and 16S amplification techniques to study the diversity of fungal and bacterial endophytes in critical plant parts of this species: seeds, branchlets, and roots. Additionally, we examined the litter of this species to understand the process of branchlets from birth to litter. We uncovered a non-random distribution of endophyte diversity in which branchlets had the greatest and seeds had the lowest endophytic fungal diversity. In contrast, litter endophytic bacteria had the highest diversity, and branchlets had the lowest diversity. As for fungi, a large part of the seed microbiome was transmitted to the phyllosphere, while a large part of the bacterial microbiome in the seed was transmitted to the root. Our study provides comprehensive evidence on diversity, potential sources, and transmission pathways for non-crop microbiome assembly and has implications for the management and manipulation of the non-crop microbiome in the future.","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138496564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and physiological traits provide insights into ecological niche adaptations of mangrove endophytic Streptomyces parvulus VCCM 22513 基因组和生理特征为红树林内生细小链霉菌VCCM22513的生态位适应提供了见解

IF 3 4区生物学

Annals of Microbiology Pub Date : 2022-07-26 DOI: 10.1186/s13213-022-01684-6

N. Quach, T. Vu, Thi Lien Bui, Thi-Thanh-Xuan Le, Thi Thu An Nguyen, C. Ngo, Q. Phi

引用次数: 3

Effects of oil pollution on soil microbial diversity in the Loess hilly areas, China 石油污染对黄土丘陵区土壤微生物多样性的影响

IF 3 4区生物学

Annals of Microbiology Pub Date : 2022-07-14 DOI: 10.1186/s13213-022-01683-7

Shi, Lei, Liu, Zhongzheng, Yang, Liangyan, Fan, Wangtao

{"title":"Effects of oil pollution on soil microbial diversity in the Loess hilly areas, China","authors":"Shi, Lei, Liu, Zhongzheng, Yang, Liangyan, Fan, Wangtao","doi":"10.1186/s13213-022-01683-7","DOIUrl":"https://doi.org/10.1186/s13213-022-01683-7","url":null,"abstract":"Data support and theoretical basis for bioremediation and treatment of petroleum-contaminated soils in the Loess hills of Yan’an, northern Shaanxi. The evolutionary characteristics of soil microbial diversity and community structure under different levels of oil pollution were studied by field sampling, indoor simulation experiments, and analyzed through assays, using the mine soils from Yan’an, Shaanxi Province, as the research object. Compared with clean soil, the microbial species in contaminated soil were significantly reduced, the dominant flora changed, and the flora capable of degrading petroleum pollutants increased significantly. The soil microbial diversity and community structure differed, although not significantly, between different pollution levels, but significantly from clean soil. In the uncontaminated soil (CK), the dominant soil microbial genera were mainly Pantoea, Sphingomonas, Thiothrix, and Nocardioides. The abundance of Pseudomonas, Pedobacter, Massilia, Nocardioides, and Acinetobacter in the soil increased after oil contamination, while Thiothrix, Sphingomonas, and Gemmatimonas decreased significantly. After the soil was contaminated with petroleum, the microbial species in the soil decreased significantly, the dominant genera in the soil changed, and the relative abundance of bacteria groups capable of degrading petroleum pollutants increased. The genera that can degrade petroleum pollutants in the petroleum-contaminated soil in the study area mainly include Pseudomonas, Acinetobacter, Pedobacter, Acinetobacter, and Nocardioides, which provide a scientific basis for exploring It provides a scientific basis for exploring remediation methods suitable for petroleum-contaminated soil in this region.","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138496563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Investigation of the location and secretion features of Candida albicans enolase with monoclonal antibodies 单克隆抗体研究白色念珠菌烯醇化酶的位置和分泌特征

IF 3 4区生物学

Annals of Microbiology Pub Date : 2022-06-28 DOI: 10.1186/s13213-022-01682-8

He, Zhengxin, Piao, Jingzi, Qiu, Yanguang, Lei, Daxin, Yang, Yanhui, Shi, Lanchun, Wang, Fukun

{"title":"Investigation of the location and secretion features of Candida albicans enolase with monoclonal antibodies","authors":"He, Zhengxin, Piao, Jingzi, Qiu, Yanguang, Lei, Daxin, Yang, Yanhui, Shi, Lanchun, Wang, Fukun","doi":"10.1186/s13213-022-01682-8","DOIUrl":"https://doi.org/10.1186/s13213-022-01682-8","url":null,"abstract":"The glycolytic enzyme enolase plays important role in the pathogenesis of Candida albicans infection and has been also considered as a promising molecular marker for the diagnosis of invasive candidiasis. This study aimed to investigate the location and secretion features of Candida albicans enolase (CaEno) with a couple of specific monoclonal antibodies (mAbs). Two mAbs named 9H8 and 10H8 against CaEno were generated by fusing SP2/0 myeloma cell with the spleen lymphocytes from CaEno immunized mice. The specificity of the mAbs was then validated by Western blot and liquid chromatography-mass spectrometry (LC–MS/MS). A diverse set of experiments were conducted based on the pair of mAbs which involved immunohistochemical staining analysis, whole cell enzyme-linked immunosorbent assay (ELISA), double antibody sandwich ELISA, and confocal microscopy to analyze the possible location and secretion features of CaEno. CaEno is abundantly expressed in the cytoplasm of C. albicans blastospores and is distributed in a ring-shaped pattern along the cell wall. CaEno appeared in the hyphal C. albicans as just a “mushroom” form. CaEno was found to be weakly expressed on the surface of blastospores but constantly expressed at various stages of growth. CaEno concentrations in C. albicans blastospores culture supernatant are considerably higher than in C. albicans hyphae culture supernatant. The dynamic changes of supernatant CaEno concentration in blastospores and hyphal C. albicans exhibit distinct features, although both appear to be associated with the C. albicans growth state. When cultivated under normal circumstances, however, no apparent CaEno degradation was seen in the cell-free supernatant. Our results implied that CaEno was constantly expressed on the cell surface and its secretion features varied according to the growth stage of C. albicans. However, further experimental and theoretical studies are needed in future to identify the specific mechanisms by which this phenomenon can arise.","PeriodicalId":8069,"journal":{"name":"Annals of Microbiology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138496562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of plant waste addition as exogenous nutrients on microbial remediation of petroleum-contaminated soil 植物废弃物作为外源养分添加对石油污染土壤微生物修复的影响

IF 3 4区生物学

Annals of Microbiology Pub Date : 2022-06-19 DOI: 10.1186/s13213-022-01679-3

Lei Shi, Zhongzheng Liu, Liangyan Yang, Wangtao Fan

引用次数: 1

Variation of soil bacterial communities in a chronosequence of citrus orchard 柑桔园土壤细菌群落的时间序列变化

IF 3 4区生物学

Annals of Microbiology Pub Date : 2022-06-17 DOI: 10.1186/s13213-022-01681-9

Yaming Jin, Z. Fang, Xinguo Zhou

引用次数: 1

High-throughput sequencing analysis reveals genomic similarity in phenotypic heterogeneous Photorhabdus luminescens cell populations 高通量测序分析揭示表型异质性发光光弹虫细胞群的基因组相似性