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Problem-oriented small-group discussion in the teaching of biochemistry laboratory practicals. 问题导向的小组讨论在生物化学实验实践教学中的应用。
Biochemical education Pub Date : 2000-05-01
Das, Sinha
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引用次数: 0
Guide for authors 作者指南
Biochemical education Pub Date : 2000-05-01 Epub Date: 2000-06-28 DOI: 10.1016/S0307-4412(00)00033-9
{"title":"Guide for authors","authors":"","doi":"10.1016/S0307-4412(00)00033-9","DOIUrl":"https://doi.org/10.1016/S0307-4412(00)00033-9","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 125-126"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00033-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91995912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A practical approach to the choice of a suitable detergent and optimal conditions for solubilizing a membrane protein 一种实用的方法来选择合适的洗涤剂和最佳条件，以溶解膜蛋白
Biochemical education Pub Date : 2000-05-01 Epub Date: 2000-06-28 DOI: 10.1016/S0307-4412(99)00107-7
Hérica de Lima Santos, Pietro Ciancaglini
{"title":"A practical approach to the choice of a suitable detergent and optimal conditions for solubilizing a membrane protein","authors":"Hérica de Lima Santos,&nbsp;Pietro Ciancaglini","doi":"10.1016/S0307-4412(99)00107-7","DOIUrl":"10.1016/S0307-4412(99)00107-7","url":null,"abstract":"<div><p><span>The main objective of this laboratory practical class was to teach students how a detergent and the best experimental conditions are chosen to solubilize a given membrane protein. Kidney Na,K-ATPase was chosen as the protein of interest and anionic, neutral and zwitterionic detergents were tested. Simple laboratory experiments were designed to study the effect of the detergent on the activity of the enzyme, the effect of detergent concentration on solubilization, the effect of protein concentration on enzyme solubilization, and the effect of time and temperature of incubation during enzyme solubilization. This resulted in the selection of an appropriate detergent for the solubilization of the protein taking into account smaller inactivation factors, more effective solubilization (more effective solubilization with a better detergent–protein relationship), lower inactivation temperature and time of incubation of the membrane protein with the detergent. The results obtained showed that instantaneous incubation of Na,K-ATPase with C</span><sub>12</sub>E<sub>8</sub> (1<!--> <!-->:<!--> <!-->1 w/w) at 4°C resulted in a more efficient solubilization and had a smaller denaturing effect on the solubilized enzyme.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 178-182"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00107-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56623675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Enzyme kinetics: partial and complete uncompetitive inhibition 酶动力学:部分和完全非竞争性抑制
Biochemical education Pub Date : 2000-05-01 Epub Date: 2000-06-28 DOI: 10.1016/S0307-4412(00)00029-7
Chris G. Whiteley
{"title":"Enzyme kinetics: partial and complete uncompetitive inhibition","authors":"Chris G. Whiteley","doi":"10.1016/S0307-4412(00)00029-7","DOIUrl":"10.1016/S0307-4412(00)00029-7","url":null,"abstract":"<div><p>A graphical method for analysing enzyme data to obtain kinetic parameters, to identify the types of inhibition and the enzyme mechanisms is described. The method consists of plotting experimental data as <em>v</em>/(<em>V</em><sub>0</sub>−<em>v</em>) versus 1/(<em>I</em>) at different substrate concentrations. I is the inhibitor concentration; <em>V</em><sub>0</sub> and v are the initial rates of enzyme reaction attained by the system in the presence of a fixed amount of substrate and in the absence and presence of inhibitor respectively. Complete inhibition gives straight lines that pass through the origin while partial inhibition gives straight lines that converge on the 1/<em>I</em><span>-axis at a point away from the origin. With uncompetitive inhibition the slopes of the lines decrease with increasing substrate concentration. The kinetic parameters </span><em>K</em><sub>m</sub>, <em>K</em>′<sub>i</sub> and <em>β</em> (degree of partiality) can best be determined from respective secondary plots of slope and intercept versus reciprocal of substrate concentration.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 144-147"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00029-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21721011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple protocol to evaluate nitrogen utilisation in Saccharomyces cerevisiae 一种评价酿酒酵母菌氮利用的简单方法
Biochemical education Pub Date : 2000-05-01 Epub Date: 2000-06-28 DOI: 10.1016/S0307-4412(99)00139-9
Carlos A. Stella, Daniel A. Sáenz, Mónica S. Chianelli, Sebastián Kaminszczik
{"title":"A simple protocol to evaluate nitrogen utilisation in Saccharomyces cerevisiae","authors":"Carlos A. Stella,&nbsp;Daniel A. Sáenz,&nbsp;Mónica S. Chianelli,&nbsp;Sebastián Kaminszczik","doi":"10.1016/S0307-4412(99)00139-9","DOIUrl":"10.1016/S0307-4412(99)00139-9","url":null,"abstract":"<div><p>The present article describes a simple protocol that allows the study of nitrogen source utilisation by cells and its relation with cellular growth. The assay can be performed using the minimum of laboratory equipment.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 169-170"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00139-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21721018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Oxidation of cholinesterase-inhibiting pesticides: A simple experiment to illustrate the role of bioactivation in the toxicity of chemicals 胆碱酯酶抑制农药的氧化:一个简单的实验来说明生物活化在化学品毒性中的作用
Biochemical education Pub Date : 2000-05-01 Epub Date: 2000-06-28 DOI: 10.1016/S0307-4412(99)00148-X
M.I Arufe, J.L Romero, J.J Gamero, M.J Moreno
{"title":"Oxidation of cholinesterase-inhibiting pesticides: A simple experiment to illustrate the role of bioactivation in the toxicity of chemicals","authors":"M.I Arufe,&nbsp;J.L Romero,&nbsp;J.J Gamero,&nbsp;M.J Moreno","doi":"10.1016/S0307-4412(99)00148-X","DOIUrl":"10.1016/S0307-4412(99)00148-X","url":null,"abstract":"<div><p><span>A simple undergraduate laboratory experiment that can be used in Biochemistry and Toxicology courses to illustrate the importance of metabolic reactions in the toxicity of chemical substances is reported. It involves the experimental confirmation that oxidized phosphorothionate esters, commonly used as insecticides, are stronger cholinesterase inhibitors and therefore exhibit higher toxicity than do their sulphur analogs starting from which the first are formed by in vivo oxidative desulphuration. Two separated aliquots of a bovine blood sample are incubated with </span>parathion and paraoxon, its oxygen analog, and compared for cholinesterase activity with “normal” blood. Previously, a standard sample of paraoxon was obtained by oxidation of the thiono group of parathion with bromine vapour by reaction TLC. The comparison of the inhibitory capacity of both compounds is made by a colorimetric procedure using acetylthiocholine as substrate of the enzyme and 5,5′-dithiobis-(2-nitrobenzoic acid) as chromogen.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 174-177"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00148-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21721020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Darwin's Black Box 达尔文的黑匣子
Biochemical education Pub Date : 2000-05-01 Epub Date: 2000-06-28 DOI: 10.1016/S0307-4412(00)00010-8
Akif Uzman
{"title":"Darwin's Black Box","authors":"Akif Uzman","doi":"10.1016/S0307-4412(00)00010-8","DOIUrl":"10.1016/S0307-4412(00)00010-8","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Page 183"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00010-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56595945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interrelationship between oxidative damage and antioxidant enzyme activities: an easy and rapid experimental approach. 氧化损伤与抗氧化酶活性的相互关系:一种简单快速的实验方法。
Biochemical education Pub Date : 2000-03-01
Maté, Aledo, Pérez-Gómez, Esteban del Valle A, Segura
{"title":"Interrelationship between oxidative damage and antioxidant enzyme activities: an easy and rapid experimental approach.","authors":"Maté,&nbsp;Aledo,&nbsp;Pérez-Gómez,&nbsp;Esteban del Valle A,&nbsp;Segura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This article describes a method for determining some antioxidant enzyme activities (catalase and/or glutathione peroxidase) and the oxidative status (protein oxidative damage and/or lipid peroxidation) of human blood. However, the main objective of the work is to illustrate the relationship between antioxidant defences and oxidative damage, showing to students their correlation and the general importance of the biochemical regulation in health and diseases.</p>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"93-95"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21575434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interrelationship between oxidative damage and antioxidant enzyme activities: an easy and rapid experimental approach 氧化损伤与抗氧化酶活性的相互关系:一种简单快速的实验方法
Biochemical education Pub Date : 2000-03-01 Epub Date: 2000-08-03 DOI: 10.1016/S0307-4412(99)00099-0
Jose M. Maté, J.Carlos Aledo, Cristina Pérez-Gómez, Alicia Esteban del Valle, Jose M. Segura
{"title":"Interrelationship between oxidative damage and antioxidant enzyme activities: an easy and rapid experimental approach","authors":"Jose M. Maté,&nbsp;J.Carlos Aledo,&nbsp;Cristina Pérez-Gómez,&nbsp;Alicia Esteban del Valle,&nbsp;Jose M. Segura","doi":"10.1016/S0307-4412(99)00099-0","DOIUrl":"10.1016/S0307-4412(99)00099-0","url":null,"abstract":"<div><p>This article describes a method for determining some antioxidant enzyme activities (catalase and/or glutathione peroxidase) and the oxidative status (protein oxidative damage and/or lipid peroxidation) of human blood. However, the main objective of the work is to illustrate the relationship between antioxidant defences and oxidative damage, showing to students their correlation and the general importance of the biochemical regulation in health and diseases.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"Pages 93-95"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00099-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56623372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Integration of post-graduate studies with corporately funded biotechnology programs 研究生学习与公司资助的生物技术项目的整合
Biochemical education Pub Date : 2000-03-01 Epub Date: 2000-08-03 DOI: 10.1016/S0307-4412(99)00128-4
Jennelle Kyd
{"title":"Integration of post-graduate studies with corporately funded biotechnology programs","authors":"Jennelle Kyd","doi":"10.1016/S0307-4412(99)00128-4","DOIUrl":"10.1016/S0307-4412(99)00128-4","url":null,"abstract":"<div><p>Post graduate research students need to undertake programs that will develop their potential as a scientist. They need to acquire skills to develop scientific argument, design an experimental approach to test a hypothesis, obtain and analyse data, and effectively communicate their ideas and findings to the scientific community. Many biotechnology-based programs are now funded by industry partnerships and integrating post graduate students into these programs require special considerations.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"Pages 86-88"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00128-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21575534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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