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Biochemical education Pub Date : 2000-05-01 DOI: 10.1016/S0307-4412(00)00015-7
G.R. Parslow
{"title":"","authors":"G.R. Parslow","doi":"10.1016/S0307-4412(00)00015-7","DOIUrl":"https://doi.org/10.1016/S0307-4412(00)00015-7","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Page 157"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00015-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91977646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Problem-based learning 问题式学习
Biochemical education Pub Date : 2000-05-01 DOI: 10.1016/S0307-4412(00)00037-6
Chris Smith
{"title":"Problem-based learning","authors":"Chris Smith","doi":"10.1016/S0307-4412(00)00037-6","DOIUrl":"https://doi.org/10.1016/S0307-4412(00)00037-6","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Page 143"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00037-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136521742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Carboxyfluorescein fluorescence experiments. 羧基荧光素荧光实验。
Biochemical education Pub Date : 2000-05-01
Massou, Albigot, Prats
{"title":"Carboxyfluorescein fluorescence experiments.","authors":"Massou,&nbsp;Albigot,&nbsp;Prats","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Although fluorescence is a powerful and much used tool in Biology there are very few applications for teaching this topic in Biochemistry courses. Some classical experiments in carboxyfluorescein fluorescence are presented here, including recording a fluorescence spectrum, quenching by oxygen, effect of concentration and pH on fluorescence signal, and a Stern-Volmer representation for a dynamic quenching.</p>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"171-173"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21721019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Guide for authors. 作者指南。
Biochemical education Pub Date : 2000-05-01
{"title":"Guide for authors.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"125-126"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21721791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biochemistry and Molecular Biology Education (BAMBEd) 生物化学及分子生物学教育
Biochemical education Pub Date : 2000-05-01 DOI: 10.1016/S0307-4412(00)00032-7
Judith G Voet , Donald Voet
{"title":"Biochemistry and Molecular Biology Education (BAMBEd)","authors":"Judith G Voet ,&nbsp;Donald Voet","doi":"10.1016/S0307-4412(00)00032-7","DOIUrl":"10.1016/S0307-4412(00)00032-7","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Page 124"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00032-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21721792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Computer applications in biomolecular sciences. Part 2: bioinformatics and genome projects 生物分子科学中的计算机应用。第二部分:生物信息学和基因组计划
Biochemical education Pub Date : 2000-05-01 DOI: 10.1016/S0307-4412(99)00142-9
C.E Sansom , C.A Smith
{"title":"Computer applications in biomolecular sciences. Part 2: bioinformatics and genome projects","authors":"C.E Sansom ,&nbsp;C.A Smith","doi":"10.1016/S0307-4412(99)00142-9","DOIUrl":"10.1016/S0307-4412(99)00142-9","url":null,"abstract":"<div><p>This article defines and describes some of the basics of bioinformatics and projects aimed at sequencing entire genomes. Emphasis is placed on some of the ways in which the primary structures of nucleic acids and proteins may be investigated and analysed to gain meaningful biological information using computers and appropriate software. The importance of the world wide net and access to it is given prominence, particularly in bioinformatics research and teaching.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 127-131"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00142-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21721793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
A practical approach to the choice of a suitable detergent and optimal conditions for solubilizing a membrane protein. 一种实用的方法来选择合适的洗涤剂和最佳条件，以溶解膜蛋白。
Biochemical education Pub Date : 2000-05-01
de Lima Santos H, Ciancaglini
{"title":"A practical approach to the choice of a suitable detergent and optimal conditions for solubilizing a membrane protein.","authors":"de Lima Santos H,&nbsp;Ciancaglini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The main objective of this laboratory practical class was to teach students how a detergent and the best experimental conditions are chosen to solubilize a given membrane protein. Kidney Na,K-ATPase was chosen as the protein of interest and anionic, neutral and zwitterionic detergents were tested. Simple laboratory experiments were designed to study the effect of the detergent on the activity of the enzyme, the effect of detergent concentration on solubilization, the effect of protein concentration on enzyme solubilization, and the effect of time and temperature of incubation during enzyme solubilization. This resulted in the selection of an appropriate detergent for the solubilization of the protein taking into account smaller inactivation factors, more effective solubilization (more effective solubilization with a better detergent-protein relationship), lower inactivation temperature and time of incubation of the membrane protein with the detergent. The results obtained showed that instantaneous incubation of Na,K-ATPase with C(12)E(8) (1:1 w/w) at 4 degrees C resulted in a more efficient solubilization and had a smaller denaturing effect on the solubilized enzyme.</p>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"178-182"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21721021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Dynamic Cell, a visual journey through the cell 动态细胞，通过细胞的视觉之旅
Biochemical education Pub Date : 2000-05-01 DOI: 10.1016/S0307-4412(00)00014-5
G.R Parslow
{"title":"The Dynamic Cell, a visual journey through the cell","authors":"G.R Parslow","doi":"10.1016/S0307-4412(00)00014-5","DOIUrl":"10.1016/S0307-4412(00)00014-5","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 157-159"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00014-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56596106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
β-Galactosidase immobilization for milk lactose hydrolysis: a simple experimental and modelling study of batch and continuous reactors 乳糖水解用β-半乳糖苷酶固定化:间歇式和连续式反应器的简单实验和模型研究
Biochemical education Pub Date : 2000-05-01 DOI: 10.1016/S0307-4412(99)00097-7
J.M. Obón, M.R. Castellar, J.L. Iborra, A. Manjón
{"title":"β-Galactosidase immobilization for milk lactose hydrolysis: a simple experimental and modelling study of batch and continuous reactors","authors":"J.M. Obón,&nbsp;M.R. Castellar,&nbsp;J.L. Iborra,&nbsp;A. Manjón","doi":"10.1016/S0307-4412(99)00097-7","DOIUrl":"10.1016/S0307-4412(99)00097-7","url":null,"abstract":"<div><p>The experiment described in this paper introduces students to the practical use of an enzyme (<em>β</em>-galactosidase, or lactase) acting on a natural substrate. The enzyme is immobilized onto a cheap support, and the immobilized derivative is used in a packed-bed reactor for continuous milk lactose hydrolysis. The results are compared to those obtained for discontinuous batch reactors with soluble enzyme. A mathematical model of the two types of reactors is run, and its results are compared with the experimental data obtained.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 164-168"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00097-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21721017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Research training between 14 and 18 in Hungary 在匈牙利接受14至18岁的研究培训
Biochemical education Pub Date : 2000-05-01 DOI: 10.1016/S0307-4412(00)00026-1
Péter Csermely , Gergely Halász , Gábor Jeney , Judith Máthé , László Mikló , Dániel Solymári , Ádám Szekeres , Gabriella Tamás
{"title":"Research training between 14 and 18 in Hungary","authors":"Péter Csermely ,&nbsp;Gergely Halász ,&nbsp;Gábor Jeney ,&nbsp;Judith Máthé ,&nbsp;László Mikló ,&nbsp;Dániel Solymári ,&nbsp;Ádám Szekeres ,&nbsp;Gabriella Tamás","doi":"10.1016/S0307-4412(00)00026-1","DOIUrl":"10.1016/S0307-4412(00)00026-1","url":null,"abstract":"<div><p>The first five years of a new program to organize high-level scientific research training for gifted high school students in Hungary are described. Besides giving unique research opportunities for talented students in their most receptive age, the program already helped the establishment of almost 100 scientific research clubs in Hungarian high schools, provided a focal point for science training of high school teachers and helped regional cooperation in Central-Eastern Europe.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 132-133"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00026-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21721794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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