Molecular cell biology research communications : MCBRC最新文献_第8页

Molecular cell biology research communications : MCBRC Pub Date : 2000-06-01 DOI: 10.1006/mcbr.2000.0246

引用次数: 0

p53 Codon 72 Polymorphism as a Risk Factor in the Development of Breast Cancer p53密码子72多态性是乳腺癌发生的危险因素

Molecular cell biology research communications : MCBRC Pub Date : 2000-06-01 DOI: 10.1006/mcbr.2000.0241

E.N. Papadakis, D.N. Dokianakis, D.A. Spandidos

引用次数: 138

Normal Breast Epithelial Cells Induce Apoptosis of MCF-7 Breast Cancer Cells through a p53-Mediated Pathway 正常乳腺上皮细胞通过p53介导的途径诱导MCF-7乳腺癌细胞凋亡

Molecular cell biology research communications : MCBRC Pub Date : 2000-06-01 DOI: 10.1006/mcbr.2000.0236

Robert-Alain Toillon , Eric Adriaenssens , Danièle Wouters , Severine Lottin , Bénoni Boilly , Hubert Hondermarck , Xuefen Le Bourhis

{"title":"Normal Breast Epithelial Cells Induce Apoptosis of MCF-7 Breast Cancer Cells through a p53-Mediated Pathway","authors":"Robert-Alain Toillon , Eric Adriaenssens , Danièle Wouters , Severine Lottin , Bénoni Boilly , Hubert Hondermarck , Xuefen Le Bourhis","doi":"10.1006/mcbr.2000.0236","DOIUrl":"10.1006/mcbr.2000.0236","url":null,"abstract":"<div><p>Cancer development depends not only on the nature of the tumor cells themselves but also on the regulatory effects of various normal cells. The present study was performed to better understand the mechanism by which normal breast epithelial cells (NBEC) can control the growth of MCF-7 breast cancer cells. When MCF-7 cells were treated with NBEC conditioned medium, cell growth was inhibited in a concentration-dependent manner. This inhibition was due to an induction of apoptosis without any change in cell cycle progression. The induction of apoptosis was correlated with increased levels of p53, p21<sup>waf1</sup> and decreased levels of bcl-2. Transient transfections of MCF-7 cells with two <em>p53</em> cDNA constructs demonstrated the induction of apoptosis was mediated by endogenous p53. Taken together, our results indicate that NBEC inhibit the growth of MCF-7 breast cancer cells by inducing apoptosis in them via endogenous p53.</p></div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"3 6","pages":"Pages 338-344"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2000.0236","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21862842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Expression of Intracellular Calcium Channels and Pumps after Partial Hepatectomy in Rat 大鼠肝部分切除后细胞内钙通道和钙泵的表达

Molecular cell biology research communications : MCBRC Pub Date : 2000-06-01 DOI: 10.1006/mcbr.2000.0242

Fabrice Magnino , Marie St-Pierre , Michael Lüthi , Mauricette Hilly , Jean-Pierre Mauger , Jean-François Dufour

{"title":"Expression of Intracellular Calcium Channels and Pumps after Partial Hepatectomy in Rat","authors":"Fabrice Magnino , Marie St-Pierre , Michael Lüthi , Mauricette Hilly , Jean-Pierre Mauger , Jean-François Dufour","doi":"10.1006/mcbr.2000.0242","DOIUrl":"10.1006/mcbr.2000.0242","url":null,"abstract":"<div><p>Ca<sup>2+</sup> signals regulate many cellular functions, including proliferation. They are governed by the inositol 1,4,5-trisphosphate receptor (IP<sub>3</sub>R), the only intracellular hepatic Ca<sup>2+</sup> channel and by the endoplasmic reticulum Ca<sup>2+</sup> pumps, SERCA. To characterise their role in regeneration, expression of their isoforms was studied after 2/3 hepatectomy by real-time quantitative PCR, Western blot and binding studies. We found an early increase in the expression of the IP<sub>3</sub>R isoform 1 which contrasted with the decrease of the expression of the IP<sub>3</sub>R isoforms 2 and 3 and of SERCA3. This results in a transient switch between IP<sub>3</sub>R isoforms 1 and 2, IP<sub>3</sub>R isoform 1 becoming predominant before the first round of mitosis. Binding studies detected a 30% diminution of the IP<sub>3</sub>R population at 24 h. In conclusion, the Ca<sup>2+</sup> signalling machinery is regulated, after hepatectomy, by changes in expression of the IP<sub>3</sub>R and SERCA isoforms to adapt Ca<sup>2+</sup> signals to the regenerative state.</p></div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"3 6","pages":"Pages 374-379"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2000.0242","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21862847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Author Index for Volume 3, Number 6 第3卷第6号作者索引

Molecular cell biology research communications : MCBRC Pub Date : 2000-06-01 DOI: 10.1006/mcbr.2000.0245

引用次数: 0

Expression and Purification of Recombinant Human SPARC Produced by Baculovirus 杆状病毒重组人SPARC的表达与纯化

Molecular cell biology research communications : MCBRC Pub Date : 2000-06-01 DOI: 10.1006/mcbr.2000.0237

Amy D. Bradshaw , James A. Bassuk , A. Francki , E.Helene Sage

{"title":"Expression and Purification of Recombinant Human SPARC Produced by Baculovirus","authors":"Amy D. Bradshaw , James A. Bassuk , A. Francki , E.Helene Sage","doi":"10.1006/mcbr.2000.0237","DOIUrl":"10.1006/mcbr.2000.0237","url":null,"abstract":"<div><p>SPARC (secreted protein acidic and rich in cysteine/osteonectin/BM-40), a matrix-associated protein, disrupts cell adhesion and inhibits the proliferation of many cultured cells. We report the expression of recombinant human protein (rhSPARC) in a baculovirus expression system. This procedure routinely yields ∼1 mg of purified protein per 500 ml of culture supernate. rhSPARC produced by insect cells migrates at the appropriate molecular weight under reducing and nonreducing conditions. The rhSPARC purified from insect cell media appeared structurally similar to SPARC purified from mammalian tissue culture by the criterion of circular dichroism. In addition, a series of anti-SPARC and anti-SPARC peptide antibodies recognized insect cell rhSPARC. We also show that rhSPARC produced in this system is glycosylated and is biologically active, as assessed by inhibition of endothelial cell proliferation and induction of collagen I mRNA in mesangial cells. Significant amounts of rhSPARC can now be generated in the absence of contaminating mammalian proteins for structure/function assays of SPARC activities.</p></div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"3 6","pages":"Pages 345-351"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2000.0237","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21862843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Advanced Glycation End Products Induce Blood–Retinal Barrier Dysfunction in Normoglycemic Rats 晚期糖基化终产物诱导正常血糖大鼠血视网膜屏障功能障碍

Molecular cell biology research communications : MCBRC Pub Date : 2000-06-01 DOI: 10.1006/mcbr.2000.0243

Alan W. Stitt , Tisha Bhaduri, C.B.Tara McMullen, Thomas A. Gardiner, Desmond B. Archer

{"title":"Advanced Glycation End Products Induce Blood–Retinal Barrier Dysfunction in Normoglycemic Rats","authors":"Alan W. Stitt , Tisha Bhaduri, C.B.Tara McMullen, Thomas A. Gardiner, Desmond B. Archer","doi":"10.1006/mcbr.2000.0243","DOIUrl":"10.1006/mcbr.2000.0243","url":null,"abstract":"<div><p>Advanced glycation end products (AGEs) have been implicated in the progressive vascular dysfunction which occurs during diabetic retinopathy. In the current study we have examined the role of these adducts in blood–retinal barrier (BRB) breakdown and investigated expression of the vasopermeabilizing agent vascular endothelial growth factor (VEGF) in the retina. When normoglycemic rats were injected with AGE-modified albumin daily for up to 10 days there was widespread leakage of FITC-dextran and serum albumin from the retinal vasculature when compared to control animals treated with nonmodified albumin. Ultrastructural examination of the vasculature revealed areas of attenuation of the retinal vascular endothelium and increased vesicular organelles only in the AGE-exposed rats. Quantitative RT-PCR and <em>in situ</em> hybridization demonstrated a significant increase in retinal VEGF mRNA expression (<em>P</em> < 0.05). These results suggest that AGEs can initiate BRB dysfunction in nondiabetic rats and a concomitant increase in retinal VEGF expression. These findings may have implications for the role of AGEs in the pathogenesis of diabetic retinopathy.</p></div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"3 6","pages":"Pages 380-388"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2000.0243","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21862848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 126

Polymorphism of the 5' Untranslated Region of NHE1 Gene Associated with Type-I Diabetes 与1型糖尿病相关的NHE1基因5'非翻译区多态性

Molecular cell biology research communications : MCBRC Pub Date : 2000-06-01 DOI: 10.1006/mcbr.2000.0244

Anne Dubouix, Isabelle Gennero, Michèle Niéto, Nicole Ser, Hélène Hannaire-Broutin, Jean Pierre Tauber, Jacques Pourrat, Josette Fauvel, Philippe Barthe, Hugues Chap, Jean Pierre Salles

引用次数: 0

Cellular Responses to Sodium Butyrate Exhibit the Dominance of One Parental Phenotype in Somatic Cell Hybrids 细胞对丁酸钠的反应在体细胞杂交中表现出一种亲本表型的优势

Molecular cell biology research communications : MCBRC Pub Date : 2000-06-01 DOI: 10.1006/mcbr.2000.0235

G.Stanley Cox

{"title":"Cellular Responses to Sodium Butyrate Exhibit the Dominance of One Parental Phenotype in Somatic Cell Hybrids","authors":"G.Stanley Cox","doi":"10.1006/mcbr.2000.0235","DOIUrl":"10.1006/mcbr.2000.0235","url":null,"abstract":"<div><p>The glycoprotein hormone α-subunit (GPHα) gene is inducible by sodium butyrate (NaBtr) in nontrophoblastic tumor cell lines such as HeLa (cervical carcinoma) but not in trophoblastic tumor cell lines such as JEG-3 (choriocarcinoma). The studies summarized in this report examined the ability of NaBtr to induce GPHα expression in somatic cell hybrids between HeLa SR3<sub>hyg</sub> and JEG-3<sub>neo</sub>. The hybrid cells, pooled clones resistant to both hygromycin B and G418 sulfate, have been named JELA and were indistinguishable from the SR3 parent with regard to induction of the GPHα gene. The effects of NaBtr on cell proliferation were also similar in HeLa and JELA but different from those in JEG-3. The GPHα gene could be induced by NaBtr in the JEG-3 parent only when they were simultaneously treated with cycloheximide (CHX). The ability of NaBtr to induce GPHα in CHX-treated JEG-3 cells occurred concomitantly with a change in the electrophoretic mobility of enhancer binding proteins as determined in gel shift assays. The DNA–protein complexes generated between a trophoblast specific element (TSE) and nuclear proteins in HeLa SR3 and JELA migrated significantly more slowly than the complex generated by JEG-3 nuclear proteins. However, when nuclear extracts were prepared from CHX-treated JEG-3 cells, the complex generated with the TSE oligonucleotide migrated more slowly than the complex from untreated JEG-3 cells and coincident with the complexes produced with nuclear extracts from HeLa SR3 and JELA cells. Together, these data demonstrate that inducibility of the GPHα gene by NaBtr in JELA cell hybrids resembles that of the HeLa SR3 parent and that its inducibility in the JEG-3 parent parallels the status of an enhancer binding protein (TSEB) as judged from changes in electrophoretic mobility. The results are consistent with a model in which the status of TSEB has a profound influence on the gene's response to NaBtr.</p></div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"3 6","pages":"Pages 329-337"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2000.0235","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21862841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Inhibitors of Advanced Glycation Endproducts (Part II) 晚期糖基化终产物的新型抑制剂(二)

Molecular cell biology research communications : MCBRC Pub Date : 2000-06-01 DOI: 10.1006/mcbr.2000.0239

Samuel Rahbar , Kiran Kumar Yerneni , Stephen Scott , Noe Gonzales , Iraj Lalezari

引用次数: 104