Molecular cell biology research communications : MCBRC最新文献

Nuclear Localization of the Plant Protein Ser/Thr Phosphatase PP7 植物蛋白丝氨酸/苏氨酸磷酸酶PP7的核定位

Molecular cell biology research communications : MCBRC Pub Date : 2001-11-01 DOI: 10.1006/mcbr.2001.0302

Alexandra V. Andreeva , Mikhail A. Kutuzov

{"title":"Nuclear Localization of the Plant Protein Ser/Thr Phosphatase PP7","authors":"Alexandra V. Andreeva , Mikhail A. Kutuzov","doi":"10.1006/mcbr.2001.0302","DOIUrl":"10.1006/mcbr.2001.0302","url":null,"abstract":"<div><p>Recently we identified novel plant Ser/Thr phosphatases, termed PP7, which belong to the PPP family and have no known close homologs in other kingdoms. We now addressed the intracellular location of <em>Arabidopsis thaliana</em> PP7 using GFP fusions and confocal laser scanning microscopy. PP7·GFP fusion was expressed transiently or stably in <em>Nicotiana benthamiana.</em> PP7·GFP was found to be a predominantly nuclear protein. Effects of cytoskeleton-disrupting drugs indicate that cytoskeleton may be required for efficient PP7·GFP delivery to the nucleus. Deletion of a potential nuclear localization signal in the first insert in the catalytic domain, as well as exposure to the dark, cold, high salinity and abscisic acid failed to prevent nuclear localization of PP7·GFP. Deletion of the 44 C-terminal amino acids resulted in a fusion protein located exclusively in the cytoplasm. The results suggest a possible similarity of the nuclear targeting signals in PP7 and the PP5/PPT subfamily.</p></div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"4 6","pages":"Pages 345-352"},"PeriodicalIF":0.0,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2001.0302","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91106350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Regulation of Transforming Growth Factor-β Signaling 转化生长因子-β信号的调控

Molecular cell biology research communications : MCBRC Pub Date : 2001-11-01 DOI: 10.1006/mcbr.2001.0301

Hong-Jian Zhu , Antony W. Burgess

{"title":"Regulation of Transforming Growth Factor-β Signaling","authors":"Hong-Jian Zhu , Antony W. Burgess","doi":"10.1006/mcbr.2001.0301","DOIUrl":"10.1006/mcbr.2001.0301","url":null,"abstract":"<div><p>Members of transforming growth factor β (TGF-β) family are potent regulators of multiple cellular functions, including cell proliferation, differentiation, migration, organization, and death. Yet the signaling pathways underpinning a wide array of biological activities of TGF-β appear to be deceptively simple. At every step from TGF-β secretion to activation of its target genes, the activity of TGF-β is regulated tightly, both positively and negatively. Biologically active TGF-β is cleaved from a precursor protein (latent form) and multiple process factors control the levels of active TGF-β. The efficient secretion, correct folding and deposition to the extracellular matrices require the cosecretion of latent TGF-β binding proteins (LTBPs). Once activated, TGF-β ligand signals through a heteromeric receptor complex of two distinct type I and type II serine/threonine kinase receptors TβRI and TβRII. Many factors appear to influence the formation of the active ligand–receptor complex. The relative orientation of TβRI and TβRII in the ligand–receptor complex is critical for activation: through TβRI, the activated ligand–receptor complex directly binds and phosphorylates downstream intracellular substrates, called Smads. Inhibitory Smads, Smad6 and 7, can antagonize this process. The phosphorylation of Smads leads to the formation of complexes which translocate to the nucleus. Other signaling systems can modulate the activity of the Smads: e.g., ras activity can prevent Smad complexes from entering the nucleus and specific ubiquitin ligases can target Smad for degradation. In the nucleus, the Smad complexes associate with other transcription activators or suppressors to regulate gene expression, either positively or negatively. The combined effects of the positive and/or negative TGF-β controlled gene expression together with the endogenous protein set of the target cell are responsible for the multiplicity of biological functions.</p></div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"4 6","pages":"Pages 321-330"},"PeriodicalIF":0.0,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2001.0301","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51527387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 95

Author Index for Volume 4, Number 6 第4卷第6号作者索引

Molecular cell biology research communications : MCBRC Pub Date : 2001-11-01 DOI: 10.1006/mcbr.2001.0311

引用次数: 0

Author Index for Volume 4 第4卷作者索引

Molecular cell biology research communications : MCBRC Pub Date : 2001-11-01 DOI: 10.1006/mcbr.2001.0312

引用次数: 0

IRF-1-Mediated CAS Expression Enhances Interferon-γ-Induced Apoptosis of HT-29 Colon Adenocarcinoma Cells irf -1介导的CAS表达增强干扰素γ诱导的HT-29结肠癌细胞凋亡

Molecular cell biology research communications : MCBRC Pub Date : 2001-11-01 DOI: 10.1006/mcbr.2001.0303

Ming-Chung Jiang , Tai-Lang Lin, Tao-Lin Lee, Hsin-Tien Huang, Ching-Liang Lin, Ching-Fong Liao

{"title":"IRF-1-Mediated CAS Expression Enhances Interferon-γ-Induced Apoptosis of HT-29 Colon Adenocarcinoma Cells","authors":"Ming-Chung Jiang , Tai-Lang Lin, Tao-Lin Lee, Hsin-Tien Huang, Ching-Liang Lin, Ching-Fong Liao","doi":"10.1006/mcbr.2001.0303","DOIUrl":"10.1006/mcbr.2001.0303","url":null,"abstract":"<div><p>The expression of CAS is reported to be upregulated in a variety of human tumor cells, and such expression correlates with the development of tumors. CAS also plays a role in apoptosis. We investigated whether CAS expression affects the susceptibility of tumor cells to IFN-γ-induced apoptosis. Our data show that IFN-γ treatment induces CAS expression in HT-29 tumor cells. IFN-γ-induced gene expression is primarily mediated by the transcriptional factor, IRF-1. Our data show that IRF-1 mediates IFN-γ-induced CAS expression. Transfection of HT-29 cells with CAS expression vector did not induce apoptosis of cells; nevertheless, CAS overexpression greatly enhanced IFN-γ-induced apoptosis of cells. CPP32 is regarded as one of the central apoptosis executioner molecules. CAS overexpression enhances IFN-γ-induced CPP32 expression. These results indicate that tumor cells highly expressing CAS may be more susceptible to apoptosis induced by reagents that are capable of inducing CAS expression. Thus, CAS may be a target for the elimination of tumors.</p></div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"4 6","pages":"Pages 353-358"},"PeriodicalIF":0.0,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2001.0303","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85630100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

c-src Tyrosine Kinase Is Associated with the Asialoglycoprotein Receptor in Human Hepatoma Cells c-src酪氨酸激酶与人肝癌细胞亚洲糖蛋白受体相关

Molecular cell biology research communications : MCBRC Pub Date : 2001-11-01 DOI: 10.1006/mcbr.2001.0299

Amy Parker , Robert J. Fallon

引用次数: 4

Identification of an Antigen Related to the Sea Urchin RNA-Binding Protein LP54 in Mammalian Central Nervous System 哺乳动物中枢神经系统海胆rna结合蛋白LP54相关抗原的鉴定

Molecular cell biology research communications : MCBRC Pub Date : 2001-11-01 DOI: 10.1006/mcbr.2001.0305

Daniele P. Romancino , Patrizia Guarneri , Caterina Cascio , Serena Dalmazio , Rosa Guarneri , Marta Di Carlo

引用次数: 0

Isolation and Characterization of pmk-(1–3): Three p38 Homologs in Caenorhabditis elegans 秀丽隐杆线虫pmk-(1-3)的分离与鉴定:三个p38同源物

Molecular cell biology research communications : MCBRC Pub Date : 2001-11-01 DOI: 10.1006/mcbr.2001.0300

Kevin Berman , Jim McKay , Leon Avery , Melanie Cobb

引用次数: 59

The Tuberous Sclerosis 2 Gene Product Can Localize to Nuclei in a Phosphorylation-Dependent Manner 结节性硬化症2基因产物可以磷酸化依赖的方式定位到细胞核

Molecular cell biology research communications : MCBRC Pub Date : 2001-11-01 DOI: 10.1006/mcbr.2001.0307

Dingyuan Lou, Nicole Griffith, Daniel J. Noonan

引用次数: 37

Fibroblast Growth Factor Receptor 3 Lacking the Ig IIIb and Transmembrane Domains Secreted from Human Squamous Cell Carcinoma DJM-1 Binds to FGFs 人鳞状细胞癌分泌的缺乏Ig IIIb和跨膜结构域的成纤维细胞生长因子受体3与FGFs结合

Molecular cell biology research communications : MCBRC Pub Date : 2001-11-01 DOI: 10.1006/mcbr.2001.0306

Motoki Terada , Akio Shimizu , Nobuhiro Sato , Shin-ichi Miyakaze , Hiroshi Katayama , Misuzu Kurokawa-Seo

{"title":"Fibroblast Growth Factor Receptor 3 Lacking the Ig IIIb and Transmembrane Domains Secreted from Human Squamous Cell Carcinoma DJM-1 Binds to FGFs","authors":"Motoki Terada , Akio Shimizu , Nobuhiro Sato , Shin-ichi Miyakaze , Hiroshi Katayama , Misuzu Kurokawa-Seo","doi":"10.1006/mcbr.2001.0306","DOIUrl":"10.1006/mcbr.2001.0306","url":null,"abstract":"<div><p>The fibroblast growth factor receptors (FGFRs) are a family of transmembrane tyrosine kinases that play a key role in cell growth and tumorigenesis in response to FGFs. FGFR complexity is increased by the existence of additional isoforms generated by alternative mRNA splicing. We identified that the transcript FGFR3ΔTM, an alternatively spliced isoform of FGFR3 lacking exons encoding the C-terminal half of Ig III (IIIb) and transmembrane domains, is expressed in the human squamous carcinoma cell line DJM-1. To determine whether FGFR3ΔTM has the potential to be secreted, we analyzed the protein expression in CHOK1 cells transfected with FGFR3ΔTM cDNA and DJM-1 cells. Western blot analysis revealed that FGFR3ΔTM protein was secreted, N-glycosylated, and dimerized by an intermolecular disulfide bond. Cross-linking experiments showed that FGF1 and FGF2 were able to bind to FGFR3ΔTM, suggesting that the loss of the Ig IIIb domain may confer upon FGFR3ΔTM the ability to bind to FGF2.</p></div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"4 6","pages":"Pages 365-373"},"PeriodicalIF":0.0,"publicationDate":"2001-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2001.0306","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83598828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15