Identification of an Antigen Related to the Sea Urchin RNA-Binding Protein LP54 in Mammalian Central Nervous System

Molecular cell biology research communications : MCBRC Pub Date : 2001-11-01 DOI:10.1006/mcbr.2001.0305

Daniele P. Romancino , Patrizia Guarneri , Caterina Cascio , Serena Dalmazio , Rosa Guarneri , Marta Di Carlo

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Abstract

LP54 is an RNA-binding protein involved in localization of maternal messengers in sea urchin egg and embryos. Using a polyclonal antibody directed against Paracentrotus lividus LP54 we detected a 66-kDa cross-reacting antigen in undifferentiated and differentiated SH-SY5Y human neuroblastoma cells. After treatment of undifferentiated cells with detergent, the 66-kDa antigen was found to be enriched in the cytoskeletal fraction. By Western blot the expression of this antigen was also analyzed in regions of the CNS and in tissues of the adult rat and its exclusive presence in the hippocampus and thalamus was revealed. The immunoreactivity with P. lividus antibody against LP54 in hippocampal lysate was also confirmed throughout anti-LP54 immunoaffinity column and competition experiments. The results indicates that a related protein to the sea urchin LP54 is evolutionary conserved in mammalian CNS.

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哺乳动物中枢神经系统海胆rna结合蛋白LP54相关抗原的鉴定

LP54是一种rna结合蛋白，参与海胆卵和胚胎中母体信使的定位。利用针对lividus parcentrotus LP54的多克隆抗体，我们在未分化和分化的SH-SY5Y人神经母细胞瘤细胞中检测到66-kDa的交叉反应抗原。用洗涤剂处理未分化细胞后，发现66-kDa抗原在细胞骨架部分富集。Western blot还分析了该抗原在中枢神经系统区域和成年大鼠组织中的表达，发现其只存在于海马和丘脑中。通过抗LP54免疫亲和柱和竞争实验，证实了海马体裂解液中P. lividus抗体对LP54的免疫反应性。结果表明，与海胆LP54相关的蛋白在哺乳动物中枢神经系统中具有进化保守性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Molecular cell biology research communications : MCBRC

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