Molecular cell biology research communications : MCBRC最新文献_第3页

Exogenous Fibroblast Growth Factor-2 Induces a Transformed Phenotype in Vascular Kaposi's Sarcoma-like Cells 外源性成纤维细胞生长因子-2诱导血管性卡波西肉瘤样细胞表型转化

Molecular cell biology research communications : MCBRC Pub Date : 2000-10-01 DOI: 10.1006/mcbr.2001.0278

Ugo Cavallaro , Marco R. Soria , Roberto Montesano

{"title":"Exogenous Fibroblast Growth Factor-2 Induces a Transformed Phenotype in Vascular Kaposi's Sarcoma-like Cells","authors":"Ugo Cavallaro , Marco R. Soria , Roberto Montesano","doi":"10.1006/mcbr.2001.0278","DOIUrl":"10.1006/mcbr.2001.0278","url":null,"abstract":"<div>Vascular TTB cells derive from murine Kaposi's sarcoma-like dermal lesions and share several phenotypic features with AIDS-associated KS spindle cells. We have recently reported that fibroblast growth factor-2 (FGF-2) promotes dramatic cytoskeletal and morphological alterations in TTB cells, concomitant with the induction of an autocrine loop for hepatocyte growth factor and a relocalization of the urokinase receptor. Since all these alterations are hallmarks of cell transformation. we attempted to verify whether FGF-2 induces a transformed phenotype in TTB cells. Our results show that FGF-2-treated TTB cells acquire the ability to grow under anchorage-independent conditions. In addition, FGF-2 markedly reduced the levels of thrombospondin-1, an antiangiogenic and tumor suppressor protein, in TTB cells. Therefore, FGF-2 induces KS-like spindle cells to acquire properties characteristic of transformed cells. This suggests that FGF-2 plays a pathogenetic role in KS not only by promoting angiogenesis, but also by conferring a transformed phenotype upon KS cells. In light of previous reports on Tat-induced release of FGF-2 into the extracellular space, our findings may provide an additional mechanism for the observed synergism between Tat and FGF-2 in the pathogenesis of KS.</div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"4 4","pages":"Pages 203-205"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2001.0278","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82406852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Inflammation and Interleukin-6 Mediate Reductions in the Hepatic Expression and Transcription of the mdr1a and mdr1b Genes 炎症和白细胞介素-6介导肝脏mdr1a和mdr1b基因表达和转录的减少

Molecular cell biology research communications : MCBRC Pub Date : 2000-10-01 DOI: 10.1006/mcbr.2001.0288

Mahadeo Sukhai, Adriane Yong, Julie Kalitsky, Micheline Piquette-Miller

{"title":"Inflammation and Interleukin-6 Mediate Reductions in the Hepatic Expression and Transcription of the mdr1a and mdr1b Genes","authors":"Mahadeo Sukhai, Adriane Yong, Julie Kalitsky, Micheline Piquette-Miller","doi":"10.1006/mcbr.2001.0288","DOIUrl":"https://doi.org/10.1006/mcbr.2001.0288","url":null,"abstract":"<div>In order to elucidate the mechanisms involved in the downregulation of mdr 1 gene expression reported in experimentally-induced inflammation, we examined the effects of experimentally-induced inflammation and interleukin-(IL-) 6 on transcriptional control of the mdr1 genes in rats. RNA, nuclear extracts, and nuclear protein fractions were isolated from livers harvested from saline or turpentine-treated male Sprague–Dawley rats or from IL-6 treated or nontreated (controls) cultured rat hepatocytes. mdr gene expression and regulation was examined by RT-PCR, mRNA stability studies, nuclear run-on analysis of transcription, and gel shift analysis of promoter-transcription factor interaction. As compared to controls, significantly lower levels of mdr1a and mdr1b mRNA and significantly decreased mdr1a and mdr1b transcription rates were observed in livers isolated from the turpentine-treated rats. In vitro treatments of cultured hepatocytes with IL-6 also suppressed mdr1a and mdr1b mRNA expression and imposed similar reductions in mdr1a and mdr1b transcriptional activity. Significant effects of IL-6 on mdr1 mRNA stability were not seen. Our results indicate that reductions in mdr1 expression in experimental models of inflammation likely occurs through altered gene transcription. Furthermore, as IL-6 was found to decrease mdr1 expression and gene transcription rates in vitro, this cytokine is likely involved in the reduction of mdr1 expression that is seen in vivo during an acute inflammatory response.</div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"4 4","pages":"Pages 248-256"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2001.0288","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91635130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 77

Genetic and molecular mechanisms of cell size control. 细胞大小控制的遗传和分子机制。

Molecular cell biology research communications : MCBRC Pub Date : 2000-10-01 DOI: 10.1006/MCBR.2001.0284

J. Montagne

引用次数: 25

In Vivo Ribozyme Targeting of βAPP+ mRNAs βAPP+ mrna的体内核酶靶向

Molecular cell biology research communications : MCBRC Pub Date : 2000-10-01 DOI: 10.1006/mcbr.2001.0287

Natalia Dolzhanskaya , James Conti , George Merz , Robert B. Denman

{"title":"In Vivo Ribozyme Targeting of βAPP+ mRNAs","authors":"Natalia Dolzhanskaya , James Conti , George Merz , Robert B. Denman","doi":"10.1006/mcbr.2001.0287","DOIUrl":"10.1006/mcbr.2001.0287","url":null,"abstract":"<div>In Alzheimer's disease (AD) and Down's syndrome (DS) patients, posttranscriptional alterations of sequences encoded by exon 9 and exon 10 of the β-amyloid precursor protein (βAPP) mRNA result in mutant proteins (βAPP+) that colocalize with neurofibrillary tangles and senile plaques. These aberrant messages may contribute to the development of sporadic or late-onset Alzheimer's disease; thus, eliminating them or attenuating their expression could significantly benefit AD patients. In the present work, self-cleaving hammerhead ribozymes targeted to βAPP exon 9 (Rz9) and βAPP+ mutant exon 10 (Rz10) were examined for their ability to distinguish between βAPP and βAPP+ mRNA. In transiently transfected A-204 cells, quantitative confocal fluorescence microscopy showed that Rz9 preferentially lowered endogenous βAPP. In contrast, in transient cotransfection experiments with βAPP+ mRNAs containing a wild-type exon 9 and mutant exon 10 (βAPP-9/βAPP-10+1), or a mutant exon 9 and wild-type exon 10 (βAPP-9+1/βAPP-10) we found that Rz9 and Rz10 preferentially reduced βAPP+-mutant exon 10 mRNA in a concentration and a ribozyme-dependent manner.</div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"4 4","pages":"Pages 239-247"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2001.0287","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51527381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

BAP37 and Prohibitin Are Specifically Recognized by an SV40 T Antigen Antibody BAP37和Prohibitin被SV40 T抗原抗体特异性识别

Molecular cell biology research communications : MCBRC Pub Date : 2000-10-01 DOI: 10.1006/mcbr.2001.0281

Alison J. Darmon , Parmjit S. Jat

引用次数: 7

An Open Chromatin Structure in a Liver-Specific Enhancer That Confers High Level Expression to Human Apolipoprotein B Transgenes in Mice 肝脏特异性增强子中的开放染色质结构，在小鼠中高水平表达人载脂蛋白B转基因

Molecular cell biology research communications : MCBRC Pub Date : 2000-10-01 DOI: 10.1006/mcbr.2001.0279

Beatriz Levy-Wilson , Bernhard Paulweber , Travis J Antes , Sheryl A Goodart , Soon-Youl Lee

{"title":"An Open Chromatin Structure in a Liver-Specific Enhancer That Confers High Level Expression to Human Apolipoprotein B Transgenes in Mice","authors":"Beatriz Levy-Wilson , Bernhard Paulweber , Travis J Antes , Sheryl A Goodart , Soon-Youl Lee","doi":"10.1006/mcbr.2001.0279","DOIUrl":"https://doi.org/10.1006/mcbr.2001.0279","url":null,"abstract":"<div>A number of DNaseI-hypersensitive (DH) sites have been mapped within a regulatory region situated upstream of the human apolipoprotein B (apoB) promoter (−5262 to −899) that is required for high level expression of human apoB transgenes in the livers of mice. These DH sites were observed in nuclei from transcriptionally active liver-derived HepG2 cells, but were absent from transcriptionally inactive HeLa cell nuclei. Several nuclear protein binding sites were detected in the DNaseI-hypersensitive region by DNaseI footprinting with HepG2 nuclear extracts, representing putative binding sites for the liver-specific activators. The locations of binding sites for these transcription factors were revealed via computer analysis of the DNA sequence of this region against a transcription factor database. Many micrococcal nuclease hypersensitive (MH) sites were also observed in nuclei from HepG2 cells but not in HeLa cell nuclei, implying that in hepatic cells, nucleosomes are either absent or have been displaced from this region by the liver-specific transcriptional activators, as inferred by the correspondence between the DH sites, the MH sites and the footprints.</div>","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"4 4","pages":"Pages 206-211"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/mcbr.2001.0279","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91680525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Support vector machines for prediction of protein subcellular location. 支持向量机预测蛋白质亚细胞位置。

Molecular cell biology research communications : MCBRC Pub Date : 2000-10-01 DOI: 10.1006/MCBR.2001.0285

Y. Cai, Xiao-Jun Liu, Xue-biao Xu, Kuo-Chen Chou

{"title":"Support vector machines for prediction of protein subcellular location.","authors":"Y. Cai, Xiao-Jun Liu, Xue-biao Xu, Kuo-Chen Chou","doi":"10.1006/MCBR.2001.0285","DOIUrl":"https://doi.org/10.1006/MCBR.2001.0285","url":null,"abstract":"Support Vector Machine (SVM), which is one kind of learning machines, was applied to predict the subcellular location of proteins from their amino acid composition. In this research, the proteins are classified into the following 12 groups: (1) chloroplast, (2) cytoplasm, (3) cytoskeleton, (4) endoplasmic reticulum, (5) extracall, (6) Golgi apparatus, (7) lysosome, (8) mitochondria, (9) nucleus, (10) peroxisome, (11) plasma membrane, and (12) vacuole, which have covered almost all the organelles and subcellular compartments in an animal or plant cell. The examination for the self-consistency and the jackknife test of the SVMs method was tested for the three sets: 2022 proteins, 2161 proteins, and 2319 proteins. As a result, the correct rate of self-consistency and jackknife test reaches 91 and 82% for 2022 proteins, 89 and 75% for 2161 proteins, and 85 and 73% for 2319 proteins, respectively. Furthermore, the predicting rate was tested by the three independent testing datasets containing 2240 proteins, 2513 proteins, and 2591 proteins. The correct prediction rates reach 82, 75, and 73% for 2240 proteins, 2513 proteins, and 2591 proteins, respectively.","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"1 1","pages":"230-3"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84700017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 48

An open chromatin structure in a liver-specific enhancer that confers high level expression to human apolipoprotein b transgenes in mice. 肝脏特异性增强子中的开放染色质结构，在小鼠中高水平表达人载脂蛋白b转基因。

Molecular cell biology research communications : MCBRC Pub Date : 2000-10-01 DOI: 10.1006/MCBR.2001.0279

B. Levy-Wilson, B. Paulweber, T. Antes, S. Goodart, S. Y. Lee

{"title":"An open chromatin structure in a liver-specific enhancer that confers high level expression to human apolipoprotein b transgenes in mice.","authors":"B. Levy-Wilson, B. Paulweber, T. Antes, S. Goodart, S. Y. Lee","doi":"10.1006/MCBR.2001.0279","DOIUrl":"https://doi.org/10.1006/MCBR.2001.0279","url":null,"abstract":"A number of DNaseI-hypersensitive (DH) sites have been mapped within a regulatory region situated upstream of the human apolipoprotein B (apoB) promoter (-5262 to -899) that is required for high level expression of human apoB transgenes in the livers of mice. These DH sites were observed in nuclei from transcriptionally active liver-derived HepG2 cells, but were absent from transcriptionally inactive HeLa cell nuclei. Several nuclear protein binding sites were detected in the DNaseI-hypersensitive region by DNaseI footprinting with HepG2 nuclear extracts, representing putative binding sites for the liver-specific activators. The locations of binding sites for these transcription factors were revealed via computer analysis of the DNA sequence of this region against a transcription factor database. Many micrococcal nuclease hypersensitive (MH) sites were also observed in nuclei from HepG2 cells but not in HeLa cell nuclei, implying that in hepatic cells, nucleosomes are either absent or have been displaced from this region by the liver-specific transcriptional activators, as inferred by the correspondence between the DH sites, the MH sites and the footprints.","PeriodicalId":80086,"journal":{"name":"Molecular cell biology research communications : MCBRC","volume":"148 1","pages":"206-11"},"PeriodicalIF":0.0,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77898850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Glutathione Transport System in NIH3T3 Fibroblasts NIH3T3成纤维细胞中的谷胱甘肽转运系统

Molecular cell biology research communications : MCBRC Pub Date : 2000-10-01 DOI: 10.1006/mcbr.2001.0280

Fabio Favilli, Serena Catarzi, Teresa Iantomasi, Maria T Vincenzini

引用次数: 4

Oligomeric status of the dihydropyridine receptor in aged skeletal muscle. 老年骨骼肌中二氢吡啶受体的寡聚状态。

Molecular cell biology research communications : MCBRC Pub Date : 2000-10-01 DOI: 10.1006/MCBR.2001.0282

M. Ryan, B. Carlson, K. Ohlendieck

引用次数: 24