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Journal of the International Federation of Clinical Chemistry最新文献

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Bioluminescence-enhanced immunoassays: an ultrasensitive method. 生物发光增强免疫测定:一种超灵敏的方法。
Journal of the International Federation of Clinical Chemistry Pub Date : 1993-04-01
R Geiger, W Miska, P Kuss
{"title":"Bioluminescence-enhanced immunoassays: an ultrasensitive method.","authors":"R Geiger,&nbsp;W Miska,&nbsp;P Kuss","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Ultrasensitive bioluminescence immunoassays for the determination of peptides and proteins are illustrated in this paper with the enzyme immunological determination of total IgE in human serum. The usable range of standard curves is from 5 pg to 5000 pg per liter. The intra- and interassay coefficients of variation of this test are in the same range as obtained for chromogenic enzyme immunoassays.</p>","PeriodicalId":80043,"journal":{"name":"Journal of the International Federation of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21043199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structure and genetic engineering of antigens and antibodies. Applications in immunoassays. 抗原和抗体的结构和基因工程。免疫分析中的应用。
Journal of the International Federation of Clinical Chemistry Pub Date : 1993-04-01
P Vihko, C Wagener
{"title":"Structure and genetic engineering of antigens and antibodies. Applications in immunoassays.","authors":"P Vihko,&nbsp;C Wagener","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":80043,"journal":{"name":"Journal of the International Federation of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21020491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Coagulation testing in the clinical chemistry laboratory. 临床化学实验室的凝血试验。
Journal of the International Federation of Clinical Chemistry Pub Date : 1993-04-01
A D'Angelo
{"title":"Coagulation testing in the clinical chemistry laboratory.","authors":"A D'Angelo","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":80043,"journal":{"name":"Journal of the International Federation of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21020492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Basic features of biomedical assays. 生物医学试验的基本特征。
Journal of the International Federation of Clinical Chemistry Pub Date : 1993-02-01
D R Bangham
{"title":"Basic features of biomedical assays.","authors":"D R Bangham","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>All biomedical assays have certain aspects in common, the most striking of which is that they generally involve protein-binding. Ligand-ligator interactions and the resulting response form the basis of most assays. This paper reviews the advantages and limitations of different types of assays ranging from long-term bioassays to in vitro assays using cell-free components. Consideration of these aspects helps in the understanding of the different assays, selection of appropriate assays for different applications, and the better interpretation of assay results. Clinical chemists, hematologists, endocrinologists, immunologists, and scientists from other disciplines have each developed their own measurement methods and units. This paper seeks to identify those aspects and principles common to assays in all such disciplines, concentrating on protein-binding systems. Understanding what and how different assays measure may clarify why different assay methods often give different results.</p>","PeriodicalId":80043,"journal":{"name":"Journal of the International Federation of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21020475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genes in the diagnosis of malignant disease. IFCC Scientific Division Committee on Molecular Biology Techniques. 恶性疾病的基因诊断。IFCC科学部分子生物学技术委员会。
Journal of the International Federation of Clinical Chemistry Pub Date : 1992-12-01
C Wagener
{"title":"Genes in the diagnosis of malignant disease. IFCC Scientific Division Committee on Molecular Biology Techniques.","authors":"C Wagener","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":80043,"journal":{"name":"Journal of the International Federation of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21018255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The application of molecular biology techniques to the diagnosis of hyperlipidaemia and other risk factors for cardiovascular disease. Committee on Molecular Biology Techniques in Clinical Chemistry of the International Federation of Clinical Chemistry (IFCC). 分子生物学技术在高脂血症及其他心血管疾病危险因素诊断中的应用。国际临床化学联合会临床化学分子生物学技术委员会。
Journal of the International Federation of Clinical Chemistry Pub Date : 1992-12-01
S E Humphries
{"title":"The application of molecular biology techniques to the diagnosis of hyperlipidaemia and other risk factors for cardiovascular disease. Committee on Molecular Biology Techniques in Clinical Chemistry of the International Federation of Clinical Chemistry (IFCC).","authors":"S E Humphries","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":80043,"journal":{"name":"Journal of the International Federation of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21018254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diagnostic applications of repetitive DNA sequences. 重复DNA序列的诊断应用。
Journal of the International Federation of Clinical Chemistry Pub Date : 1992-12-01
J T Epplen
{"title":"Diagnostic applications of repetitive DNA sequences.","authors":"J T Epplen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Different diagnostic applications are discussed for various categories of repetitive DNA sequences. Since all eukaryotes are characterized by genomic redundancy, these sensitive, rapid, and comparatively simple techniques are revolutionizing many fields in clinical and experimental diagnostics. In addition to individuality testing in humans and animals, for example, eukaryotic infectious agents can be identified and tumors classified with respect to genomic changes. The forensic as well as animal and plant breeding sciences have already adopted the DNA technology involving repetitive sequence tools.</p>","PeriodicalId":80043,"journal":{"name":"Journal of the International Federation of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21044097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Problems for improving performance in immunoassay. 提高免疫测定性能的问题。
Journal of the International Federation of Clinical Chemistry Pub Date : 1992-09-01
K Miyai, C P Price
{"title":"Problems for improving performance in immunoassay.","authors":"K Miyai,&nbsp;C P Price","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":80043,"journal":{"name":"Journal of the International Federation of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21023486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recommendation on sampling, transport, and storage for the determination of the concentration of ionized calcium in whole blood, plasma, and serum. IFC Scientific Division, Working Group on Ion-Selective Electrodes (WGSE). 全血、血浆和血清中离子钙浓度测定的取样、运输和储存建议。国际金融公司科学司，离子选择电极工作组。
Journal of the International Federation of Clinical Chemistry Pub Date : 1992-09-01
A B Boink, B M Buckley, T F Christiansen, A K Covington, A H Maas, O Müller-Plathe, C Sachs, O Siggaard-Andersen
{"title":"Recommendation on sampling, transport, and storage for the determination of the concentration of ionized calcium in whole blood, plasma, and serum. IFC Scientific Division, Working Group on Ion-Selective Electrodes (WGSE).","authors":"A B Boink,&nbsp;B M Buckley,&nbsp;T F Christiansen,&nbsp;A K Covington,&nbsp;A H Maas,&nbsp;O Müller-Plathe,&nbsp;C Sachs,&nbsp;O Siggaard-Andersen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The substance concentration of ionized calcium (cCa 2+) in blood, plasma, or serum preanalytically may be affected by pH changes of the sample, calcium binding by heparin, and dilution by the anticoagulant solution. pH changes in whole blood can be minimized by anaerobic sampling to avoid loss of CO 2, by measuring as soon as possible, or by storing the sample in iced water to avoid lactic acid formation. cCa 2+ and pH should be determined simultaneously. Plasma or serum: If centrifuged in a closed tube and measured immediately, the pH of the sample will be close to the original value. If there has been a delay between centrifugation and measurement, causing substantial loss of CO 2, equilibration of the sample with a gas mixture corresponding to pCO 2 = 5.3 kPa prior to the measurement is recommended. Conversion of the measured values to cCa 2+ (7.4) is only valid if the pH is in the range 7.2-7.6. Ca 2+ binding by heparin can be minimized by using either of the following: 1) a final concentration of sodium or lithium heparinate of 15 IU/mL blood or less; or 2) calcium-titrated heparin with a final concentration of less than 50 IU/mL blood. Dilution effect can be avoided by use of dry heparin in capillaries or syringes.(ABSTRACT TRUNCATED AT 250 WORDS)</p>","PeriodicalId":80043,"journal":{"name":"Journal of the International Federation of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21020645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Accuracy and precision of a robotic sample processor. 机器人样品处理机的准确度和精密度。
Journal of the International Federation of Clinical Chemistry Pub Date : 1992-09-01
D A Armbruster, L C Hawes, C T Winter
{"title":"Accuracy and precision of a robotic sample processor.","authors":"D A Armbruster,&nbsp;L C Hawes,&nbsp;C T Winter","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The accuracy and precision of the Packard Probe 1000, a computer-driven robotic sample processor, were determined using NCCLS Guideline 18-P, employing a rigorous gravimetric procedure. For volumes of 25, 100, and 200 muL, inaccuracy ranged from -0.5 to +0.6% (saline) and -0.8 to +0.7% (human serum), and imprecision from 0.1 to 0.6% (saline) and 0.02 to 0.8% (human serum), in the dip and sip mode of pipetting. For the same volumes of saline in the dispense-through pipetting mode, inaccuracy ranged from -0.1 to -0.9% and imprecision from 0.1 to 0.5%. To obtain this level of performance, the instrument's operating parameters were changed from the factory default settings. This automated liquid-handling system is capable of impressive accuracy and precision, but it must be calibrated in the same manner as any other volumetric device. Performance varies with the volume, type of liquid, and mode of pipetting. Inaccuracy of less than or equal to 1.0% and imprecision of less than =1.0% can be attained along with rapid, walkaway pipetting of calibrators, controls, and large numbers of patient samples.</p>","PeriodicalId":80043,"journal":{"name":"Journal of the International Federation of Clinical Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21020647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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