Annals of hepatology最新文献_第3页

HLA-G liver expression in chronically HIV/hepatitis C-coinfected individuals.

IF 3.7 3区医学

Annals of hepatology Pub Date : 2024-12-02 DOI: 10.1016/j.aohep.2024.101755

Fernando Crivelenti Vilar, Eduardo Donadi, Benedito Antônio Lopes da Fonseca, Janaina Cristiana de Oliveira Crispim Freitas, Ana Letícia Gomide Zanin Borducchi, Rodrigo de Carvalho Santana

{"title":"HLA-G liver expression in chronically HIV/hepatitis C-coinfected individuals.","authors":"Fernando Crivelenti Vilar, Eduardo Donadi, Benedito Antônio Lopes da Fonseca, Janaina Cristiana de Oliveira Crispim Freitas, Ana Letícia Gomide Zanin Borducchi, Rodrigo de Carvalho Santana","doi":"10.1016/j.aohep.2024.101755","DOIUrl":"10.1016/j.aohep.2024.101755","url":null,"abstract":"Introduction and objectives: Hepatitis C-induced liver disease represents a significant threat to the survival of people living with HIV. HIV/HCV-coinfected individuals have a more rapid progression to cirrhosis and its complications than HCV monoinfected patients. Although the underlying mechanisms remain unclear, HLA-G, a non-classical class I HLA molecule, has a well-recognized property to down-regulate the immune response against viruses and may favor the progression of chronic hepatitis C.Materials and methods: We analyzed HLA-G expression in 59 liver specimens of patients harboring chronic HCV and HIV coinfection and stratified the findings according to clinical and histopathological features.Results: Genotype 1 was the most prevalent (88%); the HLA-G expression was observed in 38 (64%) liver specimens, and it was more frequent in more severe stages than in milder stages of chronic hepatitis (94,1% x 55%; p<0.01). HLA-G expression in the liver was not correlated to antiviral response to hepatitis C therapy with pegylated-IFN-α plus ribavirin.Conclusions: HLA-G expression in the context of HCV/HIV coinfection is a complex process modulated by many factors. HLA-G expression may play a role in the mechanisms that facilitate disease progression and may contribute to the deterioration of the immune response against HCV in individuals living with HIV.","PeriodicalId":7979,"journal":{"name":"Annals of hepatology","volume":" ","pages":"101755"},"PeriodicalIF":3.7,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examining the prevalence of hepatic steatosis and advanced fibrosis using non-invasive measures across Canada: A national estimate using the Canadian Health Measures Survey (CHMS) from 2009-2019.

IF 3.7 3区医学

Annals of hepatology Pub Date : 2024-12-02 DOI: 10.1016/j.aohep.2024.101757

Jacob Romano, Jessica Burnside, Giada Sebastiani, Alnoor Ramji, Keyur Patel, Mark Swain, Sahar Saeed

{"title":"Examining the prevalence of hepatic steatosis and advanced fibrosis using non-invasive measures across Canada: A national estimate using the Canadian Health Measures Survey (CHMS) from 2009-2019.","authors":"Jacob Romano, Jessica Burnside, Giada Sebastiani, Alnoor Ramji, Keyur Patel, Mark Swain, Sahar Saeed","doi":"10.1016/j.aohep.2024.101757","DOIUrl":"10.1016/j.aohep.2024.101757","url":null,"abstract":"Introduction and objectives: Prevalence estimates are crucial for enhancing preparedness to prevent and manage chronic diseases. This is the first study to estimate the prevalence of hepatic steatosis and advanced fibrosis in Canada, leveraging a nationally representative survey and multiple validated non-invasive tests (NITs).Materials and methods: The Canadian Health Measures Survey (CHMS) is Canada's largest direct health measures survey, which collects data on sociodemographic, clinical factors, and blood chemistry. We determined steatosis using two NITs: the Hepatic Steatosis Index (HSI) and the NAFLD Ridge Score (NRS). The FIB-4 Index and NAFLD fibrosis score (NFS) were used to assess the risk of advanced fibrosis among adults with steatosis. Survey weights were incorporated to account for oversampling, survey nonresponse, and post-stratification.Results: Between 2009 and 2019, 1365 children (55 % males, median age 13 (IQR: 10-15) and 4664 adults (51 % males, median age 45 (IQR: 34-62), 57 % reporting weekly alcohol consumption) were included in our study. The weighted steatosis prevalence ranged from 9 to 11 % among children to 38-48 % among adults based on the NRS and HSI, respectively. Between 86-87 % of adults with type 2 diabetes and 65-72 % with hypertension had evidence of steatosis. Overall, 1.2-2.4 % of adults with steatosis were at risk of advanced liver fibrosis.Conclusions: We estimate between 1 in 3 and 1 in 2 adults have hepatic steatosis, and 195,000-406,200 are at high risk of advanced liver fibrosis in Canada. No routine screening guidelines for liver fibrosis exist in Canada, and most patients are unaware of their condition. Prevalence studies are essential for raising awareness and advocating for the inclusion of steatotic liver disease on national public health agendas.","PeriodicalId":7979,"journal":{"name":"Annals of hepatology","volume":" ","pages":"101757"},"PeriodicalIF":3.7,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-stop shop endoscopy for patients with biliary pancreatitis: A Jack of all trades?

IF 3.7 3区医学

Annals of hepatology Pub Date : 2024-12-02 DOI: 10.1016/j.aohep.2024.101752

Lukasz Krupa, Frank Lammert, Marcin Krawczyk

引用次数: 0

Free fatty acid-induced DDX3 inhibits autophagy via miR-141 upregulation in diet-induced MASLD mice model system.

IF 3.7 3区医学

Annals of hepatology Pub Date : 2024-12-02 DOI: 10.1016/j.aohep.2024.101758

Md Musa Hossain, Amit K Mishra, Ajay K Yadav, Md Ismail, Teja Naveen Sata, Amrendra K Sah, Arnab Banik, Gopal Sharma, Senthil K Venugopal

{"title":"Free fatty acid-induced DDX3 inhibits autophagy via miR-141 upregulation in diet-induced MASLD mice model system.","authors":"Md Musa Hossain, Amit K Mishra, Ajay K Yadav, Md Ismail, Teja Naveen Sata, Amrendra K Sah, Arnab Banik, Gopal Sharma, Senthil K Venugopal","doi":"10.1016/j.aohep.2024.101758","DOIUrl":"10.1016/j.aohep.2024.101758","url":null,"abstract":"Introduction and objectives: Metabolic dysfunction-associated steatotic liver disease (MASLD) is one of the primary causes of chronic liver disease and may lead to liver cirrhosis and hepatocellular carcinoma. Recent reports suggested that DEAD-box RNA helicase (DDX3) acts as a sensor of free fat accumulation and may modulate the pathogenesis via miRNAs. Hence, we hypothesized that DDX3 might modulate MASLD progression via miRNA-141-mediated inhibition of Sirt-1 and autophagy.Materials and methods: RNA and total protein were isolated from free fatty acid-treated HepG2 cells or CDAA-fed C57BL/6 mice (6 mice per group) for 6, 18, 32, or 54 weeks. The cells were transfected with DDX3 or miR-141 or siRNA to DDX3, and Western blots for autophagy markers were performed.Results: The FFAs induced the DDX3 and miRNA-141 expression, while downregulating Sirt-1, beclin-1, Atg7, and LC3-II. Overexpression of DDX3 resulted in increased miRNA-141. Overexpression of DDX3 or miRNA-141 downregulated Sirt-1 expression and autophagy marker proteins, while these effects were reversed with siRNA to DDX3. The expression of both DDX3 and miRNA-141 was significantly increased, while autophagy markers were downregulated in CDAA-fed mice.Conclusions: These results confirmed that FFA-induced DDX3 induced the expression of miRNA-141, which in turn targeted Sirt-1 and decreased autophagy.","PeriodicalId":7979,"journal":{"name":"Annals of hepatology","volume":" ","pages":"101758"},"PeriodicalIF":3.7,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dual role of HCV protein expression in communication between host cells presents potential applications in the treatment of liver fibrosis.

IF 3.7 3区医学

Annals of hepatology Pub Date : 2024-11-29 DOI: 10.1016/j.aohep.2024.101747

Junxi Liu, Boda Zhou

引用次数: 0

Exosomal linc00152 intensifies the crosstalk between cholangiocarcinoma cells and cancer-associated fibroblasts.

IF 3.7 3区医学

Annals of hepatology Pub Date : 2024-11-29 DOI: 10.1016/j.aohep.2024.101745

Min Hu, Yaxuan Niu, Jinlin Wang, Xiao Chen, Gang Li

{"title":"Exosomal linc00152 intensifies the crosstalk between cholangiocarcinoma cells and cancer-associated fibroblasts.","authors":"Min Hu, Yaxuan Niu, Jinlin Wang, Xiao Chen, Gang Li","doi":"10.1016/j.aohep.2024.101745","DOIUrl":"10.1016/j.aohep.2024.101745","url":null,"abstract":"Introduction and objectives: Cholangiocarcinoma is a highly lethal carcinoma. Exosomes derived from cancer-associated fibroblasts (CAFs) serve key roles in the crosstalk between CAFs and cancer cells. Exploring the roles of CAF-derived exosomes and the mechanisms contribute to a better understanding of the development of cholangiocarcinoma.Materials and methods: Carcinoma and para-carcinoma tissues were collected from patients. Exosomes were isolated from CAFs and characterized by transmission electron microscopy, dynamic light scattering and western blot. Cholangiocarcinoma cells were cocultured with CAF-derived exosomes, and its proliferation, migration and invasion were evaluated with CCK-8, EdU incorporation and transwell assays, respectively. The interaction between a long non-coding RNA linc00152 and an RNA-binding protein hnRNPA2B1 was determined with RNA immunoprecipitation and RNA pull-down. The ubiquitination of hnRNPA2B1 was examined with western blot.Results: Linc00152 was highly expressed in cholangiocarcinoma tissues and cells, and its increased expression was associated with advanced tumor stage and poor prognosis. Linc00152 was highly enriched in CAFs and CAF-derived exosomes. CAF-derived exosomes promoted cholangiocarcinoma cell proliferation, migration, and invasion by delivering linc00152. Further analysis showed that hnRNPA2B1 recruited linc00152 and enhanced its loading into exosomes. The interaction between hnRNPA2B1 and linc00152 was identified, and linc00152 repressed the proteasome-dependent degradation of hnRNPA2B1 in cholangiocarcinoma cells. The oncogenic activities of linc00152 in cholangiocarcinoma cells were dependent on hnRNPA2B1 upregulation.Conclusions: CAF-derived exosomes harboring linc00152 enhance malignancy in cholangiocarcinoma, identifying a novel role of exosomal linc00152 for intensifying the crosstalk between CAFs and cholangiocarcinoma cells.","PeriodicalId":7979,"journal":{"name":"Annals of hepatology","volume":" ","pages":"101745"},"PeriodicalIF":3.7,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brain reserve in hepatic encephalopathy: Pathways of damage and preventive strategies through lifestyle and therapeutic interventions.

IF 3.7 3区医学

Annals of hepatology Pub Date : 2024-11-29 DOI: 10.1016/j.aohep.2024.101740

Jacqueline Cordova-Gallardo, Andres Manuel Vargas-Beltran, Samantha Melanie Armendariz-Pineda, Jesus Ruiz-Manriquez, Javier Ampuero, Aldo Torre

引用次数: 0

Differentially expressed genes in Huh7 cells during co-culture with LX2 cells demonstrating dynamics of intercellular communication.

IF 3.7 3区医学

Annals of hepatology Pub Date : 2024-11-29 DOI: 10.1016/j.aohep.2024.101746

Tania G Heredia-Torres, Veronica Alvarado-Martínez, Ana R Rincón-Sánchez, Sonia A Lozano-Sepúlveda, Kame A Galán-Huerta, Daniel Arellanos-Soto, Ana M Rivas-Estilla

引用次数: 0

Integrated analyses and a novel nomogram for the prediction of significant fibrosis in patients.

IF 3.7 3区医学

Annals of hepatology Pub Date : 2024-11-29 DOI: 10.1016/j.aohep.2024.101744

Mengxin Lu, Shuai Tao, Xinyan Li, Qunling Yang, Cong Du, Weijia Lin, Shuangshuang Sun, Conglin Zhao, Neng Wang, Qiankun Hu, Yuxian Huang, Qiang Li, Yi Zhang, Liang Chen

{"title":"Integrated analyses and a novel nomogram for the prediction of significant fibrosis in patients.","authors":"Mengxin Lu, Shuai Tao, Xinyan Li, Qunling Yang, Cong Du, Weijia Lin, Shuangshuang Sun, Conglin Zhao, Neng Wang, Qiankun Hu, Yuxian Huang, Qiang Li, Yi Zhang, Liang Chen","doi":"10.1016/j.aohep.2024.101744","DOIUrl":"10.1016/j.aohep.2024.101744","url":null,"abstract":"Introduction and objectives: This study aimed to explore the key genes involved in the pathophysiological process of liver fibrosis and develop a novel predictive model for noninvasive assessment of significant liver fibrosis patients.Patients and methods: Differentially expressed genes (DEGs) were identified using the Limma package. The hub genes were explored using the CytoHubba plugin app and validated in GEO datasets and cell models. Furthermore, serum LTBP2 was measured in liver fibrosis (LF) patients with biopsy-proven by ELISA. All patients' clinical characteristics and laboratory results were analyzed. Finally, multivariate logistic regression analysis was used to construct the model for visualization by nomogram. Area under the receiver operating characteristic curve (AUROC) analysis, calibration curves, and decision curve analysis (DCA) certify the accuracy of the nomogram.Results: RNA sequencing was performed on the liver tissues of 66 biopsy-proven HBV-LF patients. After multiple analyses and in vitro simulation of HSC activation, LTBP2 was found to be the most associated with HSC activation regardless of the causes. Serum LTBP2 expression was measured in 151 patients with biopsy, and LTBP2 was found to increase in parallel with the fibrosis stage. Multivariate logistic regression analysis showed that LTBP2, PLT and AST levels were demonstrated as the independent prediction factors. A nomogram that included the three factors was tabled to evaluate the probability of significant fibrosis occurrence. The AUROC of the nomogram model was 0.8690 in significant fibrosis diagnosis.Conclusions: LTBP2 may be a new biomarker for liver fibrosis patients. The nomogram showed better diagnostic performance in patients.","PeriodicalId":7979,"journal":{"name":"Annals of hepatology","volume":" ","pages":"101744"},"PeriodicalIF":3.7,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges in the management of alcohol-associated liver disease in Latin America.

IF 3.7 3区医学

Annals of hepatology Pub Date : 2024-11-29 DOI: 10.1016/j.aohep.2024.101748

Francisco Idalsoaga, Luis Antonio Diaz, Gustavo Ayares, Marco Arrese, Juan Pablo Arab

引用次数: 0