Skin pharmacology and applied skin physiology最新文献_第2页

Relationship of hairless mouse skin surface temperature to wound severity and maturation time. 无毛小鼠皮肤表面温度与伤口严重程度及成熟时间的关系。

Skin pharmacology and applied skin physiology Pub Date : 2003-09-01 DOI: 10.1159/000072071

S K Govil, A J Flynn, G L Flynn, C Ackermann

{"title":"Relationship of hairless mouse skin surface temperature to wound severity and maturation time.","authors":"S K Govil, A J Flynn, G L Flynn, C Ackermann","doi":"10.1159/000072071","DOIUrl":"https://doi.org/10.1159/000072071","url":null,"abstract":"Skin surface temperature (SST) changes measured on live hairless mice are presented as a simple means of following wound healing. SST is generally determined by 3 factors which are the ambient temperature, the rate of water loss from the surface of the skin and the diffusion of thermal energy from the body's core. The SST increase immediately after a burn injury reflects the amount of thermal energy absorbed by the skin surface. For burns and other injuries, the drop in SST following injury, but after thermal equilibrium has been established, provides an early indication of the degree of impairment of the skin's barrier. Three kinds of mouse skin wounds, mechanical (tape stripping), thermal and chemical (phenol application), were investigated. SST nadirs ranged from 2.1 to 4.4 degrees C with mild to full-thickness burns, respectively. Except for the earliest moments after wounding, striking parallels were noted between SST and transepidermal water loss profiles for these injuries. The SST profile over the full course of wound healing clearly indicates the severity of the injury, the stages of wound maturation and the time to complete skin healing.","PeriodicalId":79724,"journal":{"name":"Skin pharmacology and applied skin physiology","volume":"16 5","pages":"313-23"},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000072071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22521531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Mass spectrometric examinations of stratum corneum lipid models exposed to ultraviolet irradiation. 紫外线照射下角质层脂质模型的质谱分析。

Skin pharmacology and applied skin physiology Pub Date : 2003-09-01 DOI: 10.1159/000072069

H Trommer, M Plätzer, R Wolf, R H H Neubert

引用次数: 6

EEMCO guidance for the efficacy assessment of antiperspirants and deodorants. EEMCO止汗剂和除臭剂功效评估指南。

Skin pharmacology and applied skin physiology Pub Date : 2003-09-01 DOI: 10.1159/000072072

G E Piérard, P Elsner, R Marks, P Masson, M Paye

{"title":"EEMCO guidance for the efficacy assessment of antiperspirants and deodorants.","authors":"G E Piérard, P Elsner, R Marks, P Masson, M Paye","doi":"10.1159/000072072","DOIUrl":"https://doi.org/10.1159/000072072","url":null,"abstract":"Overproduction of sweat, sweaty skin and body odours are unpleasant for many social groups. Body cleansing products are designed to combat these undesirable features of skin. In addition, antiperspirant and deodorant products are more specifically used in the underarm site by a large part of the adult population. Antiperspirants are offered to control emotionally triggered sweating in the armpit. Deodorants are designed to combat malodour generated from bacteria-modified sweat. This review summarizes the physiology of eccrine, apocrine and apoeccrine sweat glands. The mechanisms of action of antiperspirants and deodorants are described as well as the factors influencing their efficacies. A series of tests using various measurement methods can be used to demonstrate the efficacy of antiperspirants. These include the gravimetric method, water evaporation quantification, electrodermal measurements, staining procedures, dye injections and cyanoacrylate skin surface strippings and casting replicas. Deodorant efficacy can be evaluated by sensory assessments performed by an expert panel. Indirect support is provided by visualization of apocrine gland excretion and collection of sweat and volatile compounds. Microbiological assessments and chromatographic analysis also provide indirect information.","PeriodicalId":79724,"journal":{"name":"Skin pharmacology and applied skin physiology","volume":"16 5","pages":"324-42"},"PeriodicalIF":0.0,"publicationDate":"2003-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000072072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22521532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 65

High plasma levels of 8-methoxypsoralen following bath water delivery in dermatological patients. 皮肤病患者沐浴后血浆8-甲氧基补骨脂素水平升高。

Skin pharmacology and applied skin physiology Pub Date : 2003-09-01 DOI: 10.1159/000072070

U P Kappes, U Barta, U Merkel, A Balogh, P Elsner

引用次数: 9

Molecular skin barrier models and some central problems for the understanding of skin barrier structure and function. 分子皮肤屏障模型及其对理解皮肤屏障结构和功能的一些核心问题。

Skin pharmacology and applied skin physiology Pub Date : 2003-07-01 DOI: 10.1159/000070842

L Norlén

引用次数: 34

Platelet-activating factor antagonist WEB 2086 inhibits ultraviolet-B radiation-induced dermatitis in the human skin. 血小板活化因子拮抗剂抑制紫外线b辐射引起的人类皮肤皮炎。

Skin pharmacology and applied skin physiology Pub Date : 2003-07-01 DOI: 10.1159/000070849

E Baltás, V Trach, A Dobozy, L Kemény

{"title":"Platelet-activating factor antagonist WEB 2086 inhibits ultraviolet-B radiation-induced dermatitis in the human skin.","authors":"E Baltás, V Trach, A Dobozy, L Kemény","doi":"10.1159/000070849","DOIUrl":"https://doi.org/10.1159/000070849","url":null,"abstract":"It has been suggested that platelet-activating factor (PAF) plays a role in the pathomechanisms of various inflammatory diseases. In an experimental animal model we demonstrated earlier that a selective PAF receptor antagonist gel inhibits ultraviolet-B (UVB) light-induced edema in mouse ears. The goal of our present investigation was to determine whether locally applied WEB 2086, a selective PAF receptor antagonist, alters the dermatitis-causing effect of UVB light on human skin. We induced dermatitis in healthy volunteers by irradiating their skin with UVB light in increasing doses. The irradiated area was treated with WEB 2086 gel (3%) or with a placebo. Erythema was measured spectrophotometrically after 24 and 48 h. After both 24 and 48 h, the WEB 2086 gel significantly inhibited the UVB light-induced erythema at each radiation dose in comparison with the placebo. The PAF antagonist gel therefore proved to be effective against UVB-induced dermatitis. Our results may help to understand the relative importance of mediators in UVB-induced dermatitis and might perhaps pave the way to better therapeutic modalities in this condition.","PeriodicalId":79724,"journal":{"name":"Skin pharmacology and applied skin physiology","volume":"16 4","pages":"259-62"},"PeriodicalIF":0.0,"publicationDate":"2003-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000070849","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22417256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

UV/VIS absorbance allows rapid, accurate, and reproducible mass determination of corneocytes removed by tape stripping. 紫外/可见吸光度允许快速，准确，可重复的质量测定角质层剥离。

Skin pharmacology and applied skin physiology Pub Date : 2003-07-01 DOI: 10.1159/000070844

H-J Weigmann, U Lindemann, C Antoniou, G N Tsikrikas, A I Stratigos, A Katsambas, W Sterry, J Lademann

{"title":"UV/VIS absorbance allows rapid, accurate, and reproducible mass determination of corneocytes removed by tape stripping.","authors":"H-J Weigmann, U Lindemann, C Antoniou, G N Tsikrikas, A I Stratigos, A Katsambas, W Sterry, J Lademann","doi":"10.1159/000070844","DOIUrl":"https://doi.org/10.1159/000070844","url":null,"abstract":"The accurate determination of the mass of the horny layer removed by tape stripping is a decisive prerequisite for the application of this technique in penetration studies. A novel method using optical spectroscopy to determine the amount of stratum corneum (SC) is presented. We could show that the absorbance measured in the visible spectral range accurately reflects the mass of the SC fixed on individual tapes. Furthermore, absorbance measurement allows determination of the absolute mass of corneocyte aggregates on the removed tape strips. Topically applied substances do not disturb the spectroscopic measurements in contrast to the conventionally employed weight determination. Identical results were obtained when performing spectroscopic horny layer quantification independently in two separate institutions. Taken together, this new method is rapid, sensitive, reproducible, and accurate. We anticipate a wide application in penetration studies as well as in dermatopharmacokinetics.","PeriodicalId":79724,"journal":{"name":"Skin pharmacology and applied skin physiology","volume":"16 4","pages":"217-27"},"PeriodicalIF":0.0,"publicationDate":"2003-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000070844","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22416246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 83

Effects of oral bexarotene (targretin) on the minimal erythema dose for broadspectrum UVB light. 口服贝沙罗汀对广谱UVB光最小红斑剂量的影响。

Skin pharmacology and applied skin physiology Pub Date : 2003-07-01 DOI: 10.1159/000070846

J V Smit, E M G J De Jong, P C M Van De Kerkhof

{"title":"Effects of oral bexarotene (targretin) on the minimal erythema dose for broadspectrum UVB light.","authors":"J V Smit, E M G J De Jong, P C M Van De Kerkhof","doi":"10.1159/000070846","DOIUrl":"https://doi.org/10.1159/000070846","url":null,"abstract":"Photo(chemo)therapy and oral retinoid therapy for psoriasis or cutaneous T-cell lymphoma are frequently combined to obtain an enhanced therapeutic effect with lower safety risks. Bexarotene, a new RXR-selective retinoid (rexinoid), has been developed for the treatment of cutaneous T-cell lymphoma and has recently been investigated in the treatment of psoriasis. In the present study the UV-modulating properties of bexarotene were evaluated by assessment of the minimal erythema dose (MED) for UVB light. In 11 patients participating in a phase II study of oral bexarotene 0.5 mg/kg/day (7 patients) or 3.0 mg/kg/day (4 patients) for plaque-type psoriasis, MED tests were performed on uninvolved psoriatic skin on the lower back of the subjects before and after 12-week treatment. Clinical scores of erythema and determination of the MED 24 h after irradiation did not show statistically significant changes between the exposed areas before and after bexarotene treatment or between the two doses tested. No photosensitizing reactions were observed. This study demonstrates that a single exposure to UVB irradiation is well tolerated in patients treated with bexarotene 0.5-3.0 mg/kg/day and suggests that it is not necessary to take precautions with respect to short-term effects of sun exposure during bexarotene treatment. Further study of bexarotene photo(chemo)therapy in cutaneous T-cell lymphoma and psoriasis is warranted.","PeriodicalId":79724,"journal":{"name":"Skin pharmacology and applied skin physiology","volume":"16 4","pages":"237-41"},"PeriodicalIF":0.0,"publicationDate":"2003-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000070846","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22416244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Characteristics of skin wrinkling and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin. 单氢过氧化角鲨烯反复应用于无毛小鼠皮肤引起的皮肤起皱和皮肤变化的特征。

Skin pharmacology and applied skin physiology Pub Date : 2003-07-01 DOI: 10.1159/000070847

K Chiba, K Kawakami, T Sone, M Onoue

{"title":"Characteristics of skin wrinkling and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin.","authors":"K Chiba, K Kawakami, T Sone, M Onoue","doi":"10.1159/000070847","DOIUrl":"https://doi.org/10.1159/000070847","url":null,"abstract":"We have studied the effect of squalene monohydroperoxides (Sq-OOH), initial products of UV-peroxidated squalene, on the skin of hairless mice. Repeated topical application of 10 mM Sq-OOH to hairless mice for 15 weeks induced definite skin wrinkling. When image analysis was used to compare wrinkle formation induced by ultraviolet B (UVB) irradiation and Sq-OOH treatment, the degree of wrinkling in exposed skin was seen to be similar. However, the characteristics of wrinkles induced by either method differed markedly with regard to direction and distribution. Biochemical analysis revealed a significant decrease in collagen content per unit area and mass in Sq-OOH-treated skin, whereas no changes per unit area and decrease in collagen per unit mass were observed in UVB-irradiated skin. As for glycosaminoglycan (GAG) content per unit area, significant increases were observed in both Sq-OOH-treated skin and UVB-irradiated skin. These changes were not induced by organic hydroperoxides such as TERT-butylhydroperoxide or cumene hydroperoxide treatment. Histological observation revealed epidermal hyperplasia and dermal alterations such as collagen degradation and GAG increases in Sq-OOH-treated skin. Histological changes induced by Sq-OOH were not as pronounced as those induced by UVB irradiation. These results clearly suggest that the wrinkling and changes in dermal collagen content induced by Sq-OOH are qualitatively different to those induced by UVB exposure. This may provide a useful model for the study of skin aging, particularly with regard to collagen content.","PeriodicalId":79724,"journal":{"name":"Skin pharmacology and applied skin physiology","volume":"16 4","pages":"242-51"},"PeriodicalIF":0.0,"publicationDate":"2003-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000070847","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22416247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 60

Evaluation of the pseudo-absorption method to quantify human stratum corneum removed by tape stripping using protein absorption. 用蛋白质吸收法定量胶带剥离人角质层的伪吸收法评价。

Skin pharmacology and applied skin physiology Pub Date : 2003-07-01 DOI: 10.1159/000070845

U Lindemann, H-J Weigmann, H Schaefer, W Sterry, J Lademann

引用次数: 61