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Journal of marine biotechnology最新文献

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Antifouling activity of Indian marine invertebrates against the green mussel Perna viridis L. 印度海洋无脊椎动物对绿贻贝的防污作用。
Journal of marine biotechnology Pub Date : 1998-12-01
Devi, Vennam, Naik, Parameshwaran, Raveendran, Yeshwant
{"title":"Antifouling activity of Indian marine invertebrates against the green mussel Perna viridis L.","authors":"Devi,&nbsp;Vennam,&nbsp;Naik,&nbsp;Parameshwaran,&nbsp;Raveendran,&nbsp;Yeshwant","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Thirty-one species of marine invertebrates collected from the Indian Coast, belonging to six phyla (Porifera, Coelenterata, Annelida, Mollusca, Echinodermata, and Eurochordata) were tested for antifouling activity against the green mussel Perna viridis L. The repulsive action of the foot and production of byssus threads by green mussels were used for assaying the above activity. Subergorgia suberosa, Sinularia numerosa, Sinularia compressa, Cladiella pachyclados (phylum Coelenterata), Haliclona sp. (phylum Porifera), and Planaxis sulcatus (phylum Mollusca) have shown to be potential sources of antifoulants.</p>","PeriodicalId":79672,"journal":{"name":"Journal of marine biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20762104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Continuous and simultaneous cultivation of benthic food diatom Nitzschia sp. and abalone Haliotis sieboldii by using deep seawater. 利用深海水连续同时培养底栖食物硅藻Nitzschia sp.和三叶鲍鱼。
Journal of marine biotechnology Pub Date : 1998-12-01
Fukami, Kawai, Asada, Okabe, Hotta, Moriyama, Doi, Nishijima, Yamaguchi, Taniguchi
{"title":"Continuous and simultaneous cultivation of benthic food diatom Nitzschia sp. and abalone Haliotis sieboldii by using deep seawater.","authors":"Fukami,&nbsp;Kawai,&nbsp;Asada,&nbsp;Okabe,&nbsp;Hotta,&nbsp;Moriyama,&nbsp;Doi,&nbsp;Nishijima,&nbsp;Yamaguchi,&nbsp;Taniguchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>By using low-temperature, clean, and nutrient-rich properties of deep seawater (DSW; seawater below the euphotic layer), a continuous and simultaneous cultivation system for a benthic food diatom, Nitzschia sp., and juvenile abalone was established. Cell suspension of Nitzschia sp. was added to a bioreactor made of acrylic pipe (7 cm diameter x 50 cm long) containing short vinyl tubes (2 cm diameter x 2 cm long) as substrata. DSW collected from 320 m depth at Muroto City, in Kochi Prefecture, Japan, was supplied to the reactor and incubated under natural light (ca. 6000 lux) with a continuous DSW flow rate of 40 turnovers per hour. After growing enough benthic diatoms in the reactor, juveniles of abalone, Haliotis sieboldii (shell length ca. 10-20 mm) were put into a reactor, and cultivated simultaneously with food diatoms in the continuous flow system. During the four-month incubation, 7-month-old abalone juveniles with a shell length of 12.4 (average) +/-0.2 (SD) mm were grown to 19.4 (+/-1.7) mm in the reactor. Daily growth rates of abalones were 50-110 µm/day. These results indicate that the continuous cultivation system with DSW supports the growth of juvenile abalone without any supply of seaweed until it grows to release size.</p>","PeriodicalId":79672,"journal":{"name":"Journal of marine biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20762106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Induction of apoptosis by a marine microalgal polysaccharide in a human leukemic cell line. 海洋微藻多糖诱导人白血病细胞系细胞凋亡的研究。
Journal of marine biotechnology Pub Date : 1998-12-01
Sogawa, Matsuda, Okutani
{"title":"Induction of apoptosis by a marine microalgal polysaccharide in a human leukemic cell line.","authors":"Sogawa,&nbsp;Matsuda,&nbsp;Okutani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An extracellular polysaccharide produced by a marine microalga Gymnodinium A3 OKU-1 strain showed a strong cytotoxicity for the human leukemia K-562 cells (CC50 = 11.6 µg/ml). We revealed that this polysaccharide induced apoptosis of K-562 cells, as shown by cell morphology, nuclear fragmentation, in situ end-labeling of fragmented DNA, and internucleosomal DNA fragmentation, suggesting that the mechanism of cytotoxicity of this polymer was based on the induction of apoptosis.</p>","PeriodicalId":79672,"journal":{"name":"Journal of marine biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20762107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification and characterization of agarases from a marine bacterium, Vibrio sp. PO-303. 海洋细菌弧菌sp. PO-303中琼脂酶的纯化与特性研究。
Journal of marine biotechnology Pub Date : 1998-12-01
Araki, Hayakawa, Lu, Karita, Morishita
{"title":"Purification and characterization of agarases from a marine bacterium, Vibrio sp. PO-303.","authors":"Araki,&nbsp;Hayakawa,&nbsp;Lu,&nbsp;Karita,&nbsp;Morishita","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A marine bacterium, Vibrio sp. PO-303, produced three kinds of extracellular agarases. These enzymes were purified to homogeneity by ammonium sulfate precipitation and successive column chromatographies. The molecular masses of agarase-a, -b, and -c were estimated to be 87.5, 115, and 57 kDa by SDS-PAGE with isoelectric point of 6.6, 3.4, and 8.4, respectively. These enzymes had maximal activity at pH 6.5-7.5 and at around 38-55 degreesC. They differed in their sequences at the amino termini of the protein chains. All enzymes were inhibited completely by Hg2+. Ag+, Cu2+, and Zn2+ strongly inhibited agarase-a and -c compared with agarase-b, and the activity of agarase-c fell wide by Al3+, Fe3+, and EDTA. Agarase-a hydrolyzed agarose to give neoagarotetraose and -hexaose as predominant products, but could not cleave neoagarotetraose. The main hydrolysis products of agarase-b were neoagarobiose from agarose and neoagarooligosaccharides more than dimer. Agarase-c could not cleave neoagarohexaose.</p>","PeriodicalId":79672,"journal":{"name":"Journal of marine biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20762111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis. 利用随机扩增多态性DNA分析黑虎对虾野生种群的遗传结构。
Journal of marine biotechnology Pub Date : 1998-12-01
Tassanakajon, Pongsomboon, Jarayabhand, Klinbunga, Boonsaeng
{"title":"Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis.","authors":"Tassanakajon,&nbsp;Pongsomboon,&nbsp;Jarayabhand,&nbsp;Klinbunga,&nbsp;Boonsaeng","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Randomly amplified polymorphic DNA (RAPD) analysis was used to examine genetic variation in wild black tiger shrimp, Penaeus monodon. Specimens were collected from five geographically separated locations (Satun-Trang, Phangnga, and Medan in the Andaman Sea and Chumphon and Trad in the Gulf of Thailand). A total of 100 P. monodon individuals were investigated using seven arbitrarily selected primers. Fifty-eight (72.5%) of eighty reproducible RAPD fragments ranging in size from 200 to 2200 bp were polymorphic. The percentages of polymorphic bands of the five geographic populations investigated varied from 51.5 to 57.7%. The genetic distance between populations and UPGMA dendrograms indicated that the Medan population was genetically different from Thai P. monodon (Dij = 14.976%). Within Thailand, the Satun-Trang P. monodon was separated from the remaining geographic populations with a genetic distance of 2.632%. RAPD analysis in the present study yielded a total of 252 genotypes. A Monte Carlo analysis illustrated geographic heterogeneity in genotype frequencies within this species, suggesting that genetic population structure does exist in this taxon (P < 0.001 for all primers). Signficant differences in genotype frequencies between Thai and Indonesian (Medan) P. monodon were observed (P < 0.0001). Within Thailand, the Andaman Sea P. monodon was significantly different from that of the Gulf of Thailand (P values between 0.0000 and 0.0387), indicating population differentiation between P. monodon from these two main fishery regions of Thailand.</p>","PeriodicalId":79672,"journal":{"name":"Journal of marine biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20762109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isoprenoid synthesis gene for geranylgeranyl diphosphate synthase from a hyperthermophile, Pyrococcus sp. strain OT3. 嗜热菌热球菌菌株OT3中香叶基二磷酸合成酶类异戊二烯合成基因。
Journal of marine biotechnology Pub Date : 1998-12-01
Masuchi, Sugai, Minaka, Itoh, Itoh, Karube
{"title":"Isoprenoid synthesis gene for geranylgeranyl diphosphate synthase from a hyperthermophile, Pyrococcus sp. strain OT3.","authors":"Masuchi,&nbsp;Sugai,&nbsp;Minaka,&nbsp;Itoh,&nbsp;Itoh,&nbsp;Karube","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pyrococcus sp. strain OT3, a hyperthermophilic archaeon that was isolated by the authors was found to contain tetraether lipid mainly in the membrane lipid, which was quite different from the other hyperthermophiles (Masuchi et al. 1997). Those isoprenoids are synthesized by a family of isoprenyl diphosphate synthases from isopentenyl diphosphate to allylic diphosphates. The gene that encodes one of these families, geranylgeranyl diphosphate synthase (GGPPSase), from this strain was cloned and sequenced. This coding gene has a 960-bp (320aa) sequence. The putative Shine-Dalgarno sequence was six bases upper of start codon, exactly the same as Methanobacterium thermoautotrophicum, a methnogenic thermophile. Comparison of the amino acid sequence of 13 organisms including Eukarya, Bacteria, and Archaea showed that Archaea strains including Pyrococcus sp. strain OT3 consisted of a separate group from the others, but five conservative regions are very homologous.</p>","PeriodicalId":79672,"journal":{"name":"Journal of marine biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20762132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vibrio spp., the dominant flora in shrimp hatchery against some fish pathogenic viruses. 弧菌:对虾孵化场对某些鱼类致病性病毒的优势菌群。
Journal of marine biotechnology Pub Date : 1998-12-01
Direkbusarakom, Yoshimizu, Ezura, Ruangpan, Danayadol
{"title":"Vibrio spp., the dominant flora in shrimp hatchery against some fish pathogenic viruses.","authors":"Direkbusarakom,&nbsp;Yoshimizu,&nbsp;Ezura,&nbsp;Ruangpan,&nbsp;Danayadol","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two strains of Vibrio spp., NICA 1030 and NICA 1031, which are the dominant flora in shrimp hatcheries, were studied for antiviral activity by exposing them to the infectious hematopoietic necrosis virus (IHNV) and Oncorhynchus masou virus (OMV) before being inoculated to the CHSE-214 cells. Antiviral activities were exhibited as plaque reduction percentages of 98 and 92% for the NICA 1030 and of 99 and 62% for the NICA 1031, respectively.</p>","PeriodicalId":79672,"journal":{"name":"Journal of marine biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20761482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolites from the sponge-associated bacterium Micrococcus luteus. 海绵相关细菌黄体微球菌的代谢物。
Journal of marine biotechnology Pub Date : 1998-12-01
Bultel-Poncé, Debitus, Berge, Cerceau, Guyot
{"title":"Metabolites from the sponge-associated bacterium Micrococcus luteus.","authors":"Bultel-Poncé,&nbsp;Debitus,&nbsp;Berge,&nbsp;Cerceau,&nbsp;Guyot","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In an ongoing survey of the bioactive potential of microorganisms associated with marine invertebrates, the extract of the sponge-associated bacterial strain Micrococcus luteus was found to exhibit potent antimicrobial activity. The previously known synthetic 2,4,4'-trichloro-2'-hydroxydiphenylether was found to be responsible for the antimicrobial activity. The major metabolite isolated was a new acyl-1-(acyl-6'-mannobiosyl)-3-glycerol.</p>","PeriodicalId":79672,"journal":{"name":"Journal of marine biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20762105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antioxidative activity for human low density lipoprotein oxidation by a novel compound purified from marine microbial origin. 从海洋微生物中纯化的新型化合物对人低密度脂蛋白氧化的抗氧化活性。
Journal of marine biotechnology Pub Date : 1998-12-01
Ryu, Jeong, Gutman, Greenspan
{"title":"Antioxidative activity for human low density lipoprotein oxidation by a novel compound purified from marine microbial origin.","authors":"Ryu,&nbsp;Jeong,&nbsp;Gutman,&nbsp;Greenspan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study was designed to investigate the antioxidative activity of a substance isolated and purified from culture broth of Bacillus sp. The antioxidative substance was identified as 2,6-bis(1,1-dimethylethyl)phenol (Phen-251). The antioxidative activity of Phen-251 was higher than that of dl-tocopherol and BHA against low-density lipoprotein (LDL) oxidation by thiobarbituric acid reactive substance (TBARS). Phen-251 inhibited the copper-mediated oxidation of human LDL in a dose-dependent manner with almost complete inhibition at 40 µg/ml. Phen-251 at a concentration of 60 µg/ml also inhibited oxidation of LDL induced by J774. LDL oxidized by copper-mediated or cell-induced oxidation was degraded at a much greater rate than native LDL. These results suggested the importance of further research on Phen-251 in the investigation of atherosclerosis and free radical-induced injury.</p>","PeriodicalId":79672,"journal":{"name":"Journal of marine biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20762108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cryopreservation of Eisenia bicyclis (Laminariales, Phaeophyta) in liquid nitrogen. 双环爱森藻液氮低温保存的研究。
Journal of marine biotechnology Pub Date : 1998-12-01
Kono, Kuwano, Saga
{"title":"Cryopreservation of Eisenia bicyclis (Laminariales, Phaeophyta) in liquid nitrogen.","authors":"Kono,&nbsp;Kuwano,&nbsp;Saga","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Gametophytic cells of Eisenia bicyclis (Kjellman) Setchell were cryopreserved in liquid nitrogen (LN). Ethylene glycol was the most effective cryoprotectant when used alone, and in combination with 10% (w/v) proline improved the survival. Viability of female and male cryopreserved cells after thawing reached 62.0% and 52.6% immediately after thawing, but these levels decreased to 31.1% and 27.2% after 4 days postthawing culture. The optimal prefreezing temperature was -40 degreesC, and cells prefrozen to temperatures >-40 degreesC were damaged mainly by intracellular ice crystal formation during immersion in LN, while those prefrozen to temperatures <-40 degreesC were damaged mainly by excessive dehydration during prefreezing. The survival rates were not affected after storage in LN for at least 200 days.</p>","PeriodicalId":79672,"journal":{"name":"Journal of marine biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20762102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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