{"title":"从海洋微生物中纯化的新型化合物对人低密度脂蛋白氧化的抗氧化活性。","authors":"Ryu, Jeong, Gutman, Greenspan","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>This study was designed to investigate the antioxidative activity of a substance isolated and purified from culture broth of Bacillus sp. The antioxidative substance was identified as 2,6-bis(1,1-dimethylethyl)phenol (Phen-251). The antioxidative activity of Phen-251 was higher than that of dl-tocopherol and BHA against low-density lipoprotein (LDL) oxidation by thiobarbituric acid reactive substance (TBARS). Phen-251 inhibited the copper-mediated oxidation of human LDL in a dose-dependent manner with almost complete inhibition at 40 µg/ml. Phen-251 at a concentration of 60 µg/ml also inhibited oxidation of LDL induced by J774. LDL oxidized by copper-mediated or cell-induced oxidation was degraded at a much greater rate than native LDL. These results suggested the importance of further research on Phen-251 in the investigation of atherosclerosis and free radical-induced injury.</p>","PeriodicalId":79672,"journal":{"name":"Journal of marine biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antioxidative activity for human low density lipoprotein oxidation by a novel compound purified from marine microbial origin.\",\"authors\":\"Ryu, Jeong, Gutman, Greenspan\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study was designed to investigate the antioxidative activity of a substance isolated and purified from culture broth of Bacillus sp. The antioxidative substance was identified as 2,6-bis(1,1-dimethylethyl)phenol (Phen-251). The antioxidative activity of Phen-251 was higher than that of dl-tocopherol and BHA against low-density lipoprotein (LDL) oxidation by thiobarbituric acid reactive substance (TBARS). Phen-251 inhibited the copper-mediated oxidation of human LDL in a dose-dependent manner with almost complete inhibition at 40 µg/ml. Phen-251 at a concentration of 60 µg/ml also inhibited oxidation of LDL induced by J774. LDL oxidized by copper-mediated or cell-induced oxidation was degraded at a much greater rate than native LDL. These results suggested the importance of further research on Phen-251 in the investigation of atherosclerosis and free radical-induced injury.</p>\",\"PeriodicalId\":79672,\"journal\":{\"name\":\"Journal of marine biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of marine biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of marine biotechnology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Antioxidative activity for human low density lipoprotein oxidation by a novel compound purified from marine microbial origin.
This study was designed to investigate the antioxidative activity of a substance isolated and purified from culture broth of Bacillus sp. The antioxidative substance was identified as 2,6-bis(1,1-dimethylethyl)phenol (Phen-251). The antioxidative activity of Phen-251 was higher than that of dl-tocopherol and BHA against low-density lipoprotein (LDL) oxidation by thiobarbituric acid reactive substance (TBARS). Phen-251 inhibited the copper-mediated oxidation of human LDL in a dose-dependent manner with almost complete inhibition at 40 µg/ml. Phen-251 at a concentration of 60 µg/ml also inhibited oxidation of LDL induced by J774. LDL oxidized by copper-mediated or cell-induced oxidation was degraded at a much greater rate than native LDL. These results suggested the importance of further research on Phen-251 in the investigation of atherosclerosis and free radical-induced injury.