Biotechnology annual review最新文献_第5页

Recent advances in all-protein chromophore technology. 全蛋白发色团技术的最新进展。

Biotechnology annual review Pub Date : 2006-01-01 DOI: 10.1016/S1387-2656(06)12002-5

Mark Prescott, Jion M Battad, Pascal G Wilmann, Jamie Rossjohn, Rodney J Devenish

引用次数: 15

Convective Interaction Media (CIM)--short layer monolithic chromatographic stationary phases. 对流相互作用介质(CIM)——短层整体色谱固定相。

Biotechnology annual review Pub Date : 2005-01-01 DOI: 10.1016/S1387-2656(05)11009-6

Ales Podgornik, Ales Strancar

引用次数: 56

Cell migration/invasion assays and their application in cancer drug discovery. 细胞迁移/侵袭试验及其在抗癌药物发现中的应用。

Biotechnology annual review Pub Date : 2005-01-01 DOI: 10.1016/S1387-2656(05)11013-8

Suzanne A Eccles, Carol Box, William Court

{"title":"Cell migration/invasion assays and their application in cancer drug discovery.","authors":"Suzanne A Eccles, Carol Box, William Court","doi":"10.1016/S1387-2656(05)11013-8","DOIUrl":"https://doi.org/10.1016/S1387-2656(05)11013-8","url":null,"abstract":"Invasive capacity is the single most important trait that distinguishes benign from malignant lesions. Tumour cells, during intravasation and extravasation of blood and lymphatic channels and when establishing colonies at secondary sites, must move through tissue boundaries that normal adult cells (other than, for example activated leukocytes) do not cross. Similar mechanisms are also utilised by activated endothelial cells during the generation of new blood vessels that enable the sustained growth and dissemination of tumours. It is now increasingly recognised that these processes--cell motility and invasion--might provide a rich source of novel targets for cancer therapy and that appropriate inhibitors may restrain both metastasis and neoangiogenesis. This new paradigm demands screening assays that can rapidly and quantitatively measure cell movement and the ability to traverse physiological barriers. We also need to consider whether simple reductionist in vitro approaches can reliably model the complexity of in vivo tumour invasion/neoangiogenesis. There are both opportunities and challenges ahead in developing a balanced portfolio of assays that will be able to evaluate accurately and finally deliver novel anti-invasive agents with therapeutic potential for clinical use.","PeriodicalId":79566,"journal":{"name":"Biotechnology annual review","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-2656(05)11013-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25649632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 115

Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. 作为细胞生物学工具的四氮唑染料:对其细胞还原的新见解。

Biotechnology annual review Pub Date : 2005-01-01 DOI: 10.1016/S1387-2656(05)11004-7

Michael V Berridge, Patries M Herst, An S Tan

{"title":"Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction.","authors":"Michael V Berridge, Patries M Herst, An S Tan","doi":"10.1016/S1387-2656(05)11004-7","DOIUrl":"https://doi.org/10.1016/S1387-2656(05)11004-7","url":null,"abstract":"Tetrazolium salts have become some of the most widely used tools in cell biology for measuring the metabolic activity of cells ranging from mammalian to microbial origin. With mammalian cells, fractionation studies indicate that the reduced pyridine nucleotide cofactor, NADH, is responsible for most MTT reduction and this is supported by studies with whole cells. MTT reduction is associated not only with mitochondria, but also with the cytoplasm and with non-mitochondrial membranes including the endosome/lysosome compartment and the plasma membrane. The net positive charge on tetrazolium salts like MTT and NBT appears to be the predominant factor involved in their cellular uptake via the plasma membrane potential. However, second generation tetrazolium dyes that form water-soluble formazans and require an intermediate electron acceptor for reduction (XTT, WST-1 and to some extent, MTS), are characterised by a net negative charge and are therefore largely cell-impermeable. Considerable evidence indicates that their reduction occurs at the cell surface, or at the level of the plasma membrane via trans-plasma membrane electron transport. The implications of these new findings are discussed in terms of the use of tetrazolium dyes as indicators of cell metabolism and their applications in cell biology.","PeriodicalId":79566,"journal":{"name":"Biotechnology annual review","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-2656(05)11004-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25630132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1843

Towards quantitative biology: integration of biological information to elucidate disease pathways and to guide drug discovery. 走向定量生物学:整合生物信息以阐明疾病途径并指导药物发现。

Biotechnology annual review Pub Date : 2005-01-01 DOI: 10.1016/S1387-2656(05)11001-1

Hans Peter Fischer

{"title":"Towards quantitative biology: integration of biological information to elucidate disease pathways and to guide drug discovery.","authors":"Hans Peter Fischer","doi":"10.1016/S1387-2656(05)11001-1","DOIUrl":"https://doi.org/10.1016/S1387-2656(05)11001-1","url":null,"abstract":"Developing a new drug is a tedious and expensive undertaking. The recently developed high-throughput experimental technologies, summarised by the terms genomics, transcriptomics, proteomics and metabolomics provide for the first time ever the means to comprehensively monitor the molecular level of disease processes. The \"-omics\" technologies facilitate the systematic characterisation of a drug target's physiology, thereby helping to reduce the typically high attrition rates in discovery projects, and improving the overall efficiency of pharmaceutical research processes. Currently, the bottleneck for taking full advantage of the new experimental technologies are the rapidly growing volumes of automatically produced biological data. A lack of scalable database systems and computational tools for target discovery has been recognised as a major hurdle. In this review, an overview will be given on recent progress in computational biology that has an impact on drug discovery applications. The focus will be on novel in silico methods to reconstruct regulatory networks, signalling cascades, and metabolic pathways, with an emphasis on comparative genomics and microarray-based approaches. Promising methods, such as the mathematical simulation of pathway dynamics are discussed in the context of applications in discovery projects. The review concludes by exemplifying concrete data-driven studies in pharmaceutical research that demonstrate the value of integrated computational systems for drug target identification and validation, screening assay development, as well as drug candidate efficacy and toxicity evaluations.","PeriodicalId":79566,"journal":{"name":"Biotechnology annual review","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-2656(05)11001-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25631795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 56

Sterilisation in biotechnology. 生物技术中的灭菌。

Biotechnology annual review Pub Date : 2005-01-01 DOI: 10.1016/S1387-2656(05)11008-4

Marin Berovic

引用次数: 9

The duckweeds: a valuable plant for biomanufacturing. 浮萍:一种有价值的生物制造植物。

Biotechnology annual review Pub Date : 2005-01-01 DOI: 10.1016/S1387-2656(05)11002-3

Anne-Marie Stomp

引用次数: 109

Water ecology of Legionella and protozoan: environmental and public health perspectives. 军团菌和原生动物的水生态:环境和公共卫生观点。

Biotechnology annual review Pub Date : 2005-01-01 DOI: 10.1016/S1387-2656(05)11011-4

Paola Borella, Elisa Guerrieri, Isabella Marchesi, Moreno Bondi, Patrizia Messi

{"title":"Water ecology of Legionella and protozoan: environmental and public health perspectives.","authors":"Paola Borella, Elisa Guerrieri, Isabella Marchesi, Moreno Bondi, Patrizia Messi","doi":"10.1016/S1387-2656(05)11011-4","DOIUrl":"https://doi.org/10.1016/S1387-2656(05)11011-4","url":null,"abstract":"Ecological studies on Legionella spp. are essential to better understand their sources in the natural environments, the mechanism of their entry into man-made water systems and the factors enabling their survival and growth in aquatic habitats. Legionella spp. exhibits peculiar and multiple strategies to adapt to stressful environment conditions which normally impair other germ survival. These strategies include the ability to enter in a viable but non-cultivable (VBNC) state, to multiply intracellularly within a variety of protozoa, such as amoebae, to survive as free organisms within biofilms and to be enhanced/inhibited by the presence of other aquatic bacteria. The host-parasite interaction has been shown to be central in the pathogenesis and ecology of L. pneumophila. The bacterial-protozoan interaction contributes to the amplification of Legionella population in water systems, represents a shelter against unfavourable environmental conditions, acts as a reservoir of infection and contributes to virulence by priming the pathogen to infect human cells. Legionella is able to survive as free organism for long periods within biofilms which are widespread in man-made water systems. Biofilm provides shelter and nutrients, exhibits a remarkable resistance to biocide compounds and chlorination, thus representing ecological niches for legionella persistence in such environments. Further knowledge on biofilm-associated legionellae may lead to effective control measures to prevent legionellosis. Lastly, new perspectives in controlling legionella contamination can arise from investigations on aquatic bacteria able to inhibit legionella growth in natural and artificial water systems.","PeriodicalId":79566,"journal":{"name":"Biotechnology annual review","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-2656(05)11011-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25649630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 132

Vibrational spectroscopy for molecular characterisation and diagnosis of benign, premalignant and malignant skin tumours. 振动光谱学用于良性、癌前和恶性皮肤肿瘤的分子表征和诊断。

Biotechnology annual review Pub Date : 2005-01-01 DOI: 10.1016/S1387-2656(05)11006-0

Natalja Skrebova Eikje, Katsuo Aizawa, Yukihiro Ozaki

{"title":"Vibrational spectroscopy for molecular characterisation and diagnosis of benign, premalignant and malignant skin tumours.","authors":"Natalja Skrebova Eikje, Katsuo Aizawa, Yukihiro Ozaki","doi":"10.1016/S1387-2656(05)11006-0","DOIUrl":"https://doi.org/10.1016/S1387-2656(05)11006-0","url":null,"abstract":"Understanding the molecular, cellular and tissue changes that occur during skin carcinogenesis is central to cancer research in dermatology. The translational aspects of this field--the development of clinical applications in dermatology from the laboratory findings--aim at improving clinical diagnosis, monitoring and treatment of skin cancer. Vibrational spectroscopy, both infrared (IR) and Raman spectroscopy, would be helpful in achieving those goals, since it has been shown to have potential in characterising and discriminating tumour and dysplastic tissue from normal tissue. Clinically differential diagnosis of skin tumours is often difficult and a histopathologic analysis of skin biopsies remains the standard for diagnostic confirmation. We review and update the literature on the subject, demonstrating that the IR and Raman spectra of skin tissues provide valid and useful diagnostic information about a number of skin tumours. We also include a survey of introduced sampling methods for IR and Raman spectroscopy in dermatology, and additionally describe the differences between microscopic, macroscopic and fibreoptic diagnosis of skin cancer. Although in its early stages, we remain optimistic that vibrational spectroscopy has the potential to be fully accepted as a rapid screening tool with sufficient sensitivity and specificity for non-destructive in vitro, ex vivo and in vivo analyses by the dermatological community. Further progress toward molecular characterisation of skin cancer by vibrational spectroscopy would have important research and clinical benefits in dermatology.","PeriodicalId":79566,"journal":{"name":"Biotechnology annual review","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-2656(05)11006-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25630134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 73

The application of BacMam technology in nuclear receptor drug discovery. BacMam技术在核受体药物发现中的应用。

Biotechnology annual review Pub Date : 2005-01-01 DOI: 10.1016/S1387-2656(05)11003-5

Mohamed Boudjelal, Sarah J Mason, Roy M Katso, Jonathan M Fleming, Janet H Parham, J Patrick Condreay, Raymond V Merrihew, William J Cairns

{"title":"The application of BacMam technology in nuclear receptor drug discovery.","authors":"Mohamed Boudjelal, Sarah J Mason, Roy M Katso, Jonathan M Fleming, Janet H Parham, J Patrick Condreay, Raymond V Merrihew, William J Cairns","doi":"10.1016/S1387-2656(05)11003-5","DOIUrl":"https://doi.org/10.1016/S1387-2656(05)11003-5","url":null,"abstract":"The nuclear receptor (NR) superfamily represents a major class of drug targets for the pharmaceutical industry. Strategies for the development of novel, more selective and safer compounds aimed at these receptors are now emerging. Reporter assays have been used routinely for the identification and characterisation of NR ligands. As the NR drug development process evolves, the increase in screening demand in terms of both capacity and complexity has necessitated the development of novel assay formats with increased throughput and flexibility. BacMam technology, a modified baculovirus system for over-expressing genes of interest in mammalian cells has helped answer this requirement. BacMam has many advantages over traditional gene delivery systems including high transduction efficiencies, broad cell host range, speed, cost and ease of generation and use. As outlined in this review, the technology has shown itself to be robust and efficient in various NR assay formats including transactivation (ER alpha/beta, MR, PR and PXR) and transrepression (GR-NFkappaB). In addition, the flexibility of this system will allow greater multiplexing of receptor, reporter, and cell host combinations as NR assays become more complex in order to relate better to relevant cellular and biological systems.","PeriodicalId":79566,"journal":{"name":"Biotechnology annual review","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-2656(05)11003-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25630131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15