The Science of Nature最新文献

{"title":"Multilevel variation in pathogen susceptibility and grooming behavior in ant societies","authors":"Romain Willeput,&nbsp;Claire Detrain","doi":"10.1007/s00114-025-02055-2","DOIUrl":"10.1007/s00114-025-02055-2","url":null,"abstract":"<div>\u0000 \u0000 <p>Ant societies rely on a combination of individual and collective defenses to mitigate pathogen exposure and transmission. Certain morphological, physiological, and life-history traits are expected to influence ant susceptibility to pathogens. In species with an exclusively subterranean lifestyle, such as the yellow meadow ant <i>Lasius flavus</i>, a weakly sclerotized and depigmented cuticle is assumed to increase vulnerability to contact-transmitted entomopathogens, potentially favoring a compensatory investment in grooming behavior. However, little is known about how survival and sanitary behavior are related, and how they vary across biological levels: between species, colonies, or nestmates. In this study, we exposed <i>L. flavus</i> workers to spores of the entomopathogenic fungus <i>Beauveria bassiana</i> and compared their survival with that of five closely related, pigmented, epigeous <i>Lasius</i> species. Although pathogen susceptibility and grooming behavior differed among species, <i>L. flavus</i> workers unexpectedly showed levels comparable to those of most epigeous species. Mortality also varied significantly across <i>L. flavus</i> colonies and between functional groups of workers, with foragers experiencing higher mortality than intranidal workers, likely due to immunosenescence. Across <i>Lasius</i> species, both self-grooming and acidopore-grooming correlated positively with survival, suggesting that these behaviors contribute to pathogen resistance at the interspecific level. Among <i>L. flavus</i> colonies and functional groups of workers, the weaker, non-significant correlation between survival and grooming suggests that other immune traits may prevail at this level. Overall, our findings highlight both individual- and colony-level variation in pathogen susceptibility and emphasize the interplay between immune defenses and behavioral strategies in shaping species-specific adaptations to pathogenic threats.</p>\u0000 </div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"113 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behaviour and vocalization of Mongolian gazelle (Procapra gutturosa) in rutting harems considering the sex-specific vocal anatomy","authors":"Roland Frey,&nbsp;Ilya A. Volodin,&nbsp;Vadim E. Kirilyuk,&nbsp;Elena V. Volodina","doi":"10.1007/s00114-026-02102-6","DOIUrl":"10.1007/s00114-026-02102-6","url":null,"abstract":"<div>\u0000 \u0000 <p>We investigated behaviour and vocalization of wild-living male and female Mongolian gazelles during the winter rutting period, occurring at low temperatures, on average − 21.7˚C. We used both manual and automated audio recordings and manual video recordings of calling animals in rutting harems. Recording vocalizations with automated devices placed within harems documented a high vocal activity during the rut in both sexes. Harem males produced their series of rutting <i>barks</i> day and night. Video recordings revealed larynx retraction at each <i>bark</i> resulting in substantial vocal tract elongation. Harem females produced various <i>contact calls</i> but did not produce <i>barks</i>. Both sexes produced nasal alarm <i>snorts</i> and oral <i>running calls</i>, with widely open mouth during galloping. Mating behaviour and fights between rival males were not accompanied by any calls. Both male rutting <i>barks</i> and female <i>contact calls</i> were unusually high-frequency for animals of such size. We integrated data on behavioural postures and acoustic variables with data on sex-specific vocal anatomy, obtained during dissections of male and female specimens. We showed that Mongolian gazelle is the only studied ruminant, in which flexible vocal folds were evolutionarily lost. For both male and female larynges, we report the lack of any thin and flexible, rostrally directed mucosal parts in the glottal region suggesting that Mongolian gazelle, contrary to most other mammals, do not produce their calls by expiratory airflow-induced vocal fold vibration. The production mechanism of the high-frequency calls in Mongolian gazelle remains unclear. We discuss the evolution of vocal rutting behaviour in polygynous Mongolian gazelles.</p>\u0000 </div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"113 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147643059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptations of seal louse nits to underwater life: morphology, respiration and attachment","authors":"Anika Preuss,&nbsp;Thomas van de Kamp,&nbsp;Stanislav N. Gorb","doi":"10.1007/s00114-026-02095-2","DOIUrl":"10.1007/s00114-026-02095-2","url":null,"abstract":"<div>\u0000 \u0000 <p>Marine mammals host a variety of parasites, engaged in continuous evolutionary competition. Our understanding of parasitic insects associated with these mammals, specifically their physiological adaptations to the marine environment, remains limited. Among thirteen insect species capable of enduring extended underwater periods in open oceans is the seal louse, <i>Echinophthirius horridus</i>, which infests true seals. This species relies on adaptations to survive extreme conditions such as hypoxia, temperature variations, hydrostatic pressure, and drag forces during host dives. These conditions affect both adult lice and louse embryos within their nits. Questions arise about how these early stages survive underwater, respire inside the eggs during dives, and what limits their survival. Using CLSM, SEM, and synchrotron X-ray microtomography, we identified morphological structures consistent with a potentially specialized respiratory system in the nits, a multi-layered eggshell and structural features that may help limit water entry. Adhesive force tests showed that the nit sheath attaching eggs to seal hair is not the primary vulnerability during host dives; rather, prolonged submersion likely imposes oxygen limitation over time. This research enhances our understanding of ectoparasite-host dynamics in marine mammals and demonstrates remarkable adaptability of insects to challenging aquatic niches.</p>\u0000 </div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"113 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00114-026-02095-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147642900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant lectin from the coastal jack bean Canavalia rosea and its anti-bruchid activity towards Callosobruchus maculatus (Chrysomelidae: Coleoptera)","authors":"Meenakumari Mani,&nbsp;Nivetha Ramanathan,&nbsp;Janarthanan Sundaram","doi":"10.1007/s00114-026-02101-7","DOIUrl":"10.1007/s00114-026-02101-7","url":null,"abstract":"<div>\u0000 \u0000 <p>The insect <i>Callosobruchus maculatus</i> is a potential pest of stored products responsible for damage to legume crops by infesting their seeds, resulting in substantial impairment to their nutritional composition. A few leguminous seeds containing lectins have been found to have the most adverse effects, with the potential to hinder the metabolism of seed-feeding insects. In the present investigation, a lectin gene from the beach bean, <i>Canavalia rosea</i>, was. isolated and its open reading frame encoding 290 amino acids was cloned into pET-45b (+), transformed using BL21 (DE3) cells, and the expressed 30 kDa recombinant lectin polypeptide (<i>Cr</i>LP) was purified and characterized. Artificial seeds containing 0.1%, 0.5% and 1.0% concentrations of <i>Cr</i>LP were tested for their protective role against the infestation of <i>C. maculatus</i>, wherein a significant reduction in seed damage and adult emergence was observed. Larvae of <i>C. maculatus</i> fed on artificial seeds containing <i>Cr</i>LP were evaluated for the enzymes involved in the detoxification processes using RT-qPCR. The expression analysis of various insect detoxification enzymes revealed a significant downregulation of cytochrome p450, glutathione S-transferase, and esterase in larvae raised on all three tested concentrations of <i>Cr</i>LP. This study thus demonstrates the potential insecticidal nature of recombinant lectin polypeptide derived from the seeds of <i>C. rosea</i> towards the insect pest <i>C. maculatus</i>.</p>\u0000 </div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"113 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147642936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research advances on Cordyceps sinensis and its components in relation to omics biomarkers for the neurological disorders","authors":"Zeyu Lai,&nbsp;Biqin Zhang,&nbsp;Zixuan Fu,&nbsp;Rusong Li,&nbsp;Yichen Qian,&nbsp;Yang Zhang,&nbsp;Peng Xu,&nbsp;Yaoqiang Du","doi":"10.1007/s00114-026-02093-4","DOIUrl":"10.1007/s00114-026-02093-4","url":null,"abstract":"<div>\u0000 \u0000 <p>Cordyceps is a traditional medicinal fungus belonging to the species <i>Ophiocordyceps sinensis</i>. It grows in the alpine ecological zone of the Tibetan Plateau and exhibits dual characteristics of both insects and fungi. The primary species include <i>Cordyceps sinensis</i> and <i>Cordyceps militaris</i>. Rich in bioactive components such as cordycepin, polysaccharides, adenosine, and peptides, cordyceps demonstrates broad applications in immune regulation, anti-tumor activity, anti-inflammatory, and neuroprotection. <i>Cordyceps sinensis</i> and its components show great therapeutic potential in neurological diseases such as epilepsy, Alzheimer’s disease and Parkinson’s disease through multi-level and multi-target actions However, current research faces challenges including unclear mechanisms of action and insufficient clinical translation. In this review, we analyze the molecular mechanisms underlying cordyceps’ neuroprotective effects, including the regulating of apoptosis, improvement of mitochondrial function, and promoting of nerve repair. Utilizing network pharmacology, we explore the multi-targeted actions of cordyceps and predict the key pathways. Further we summarize the research progress in the integrated multi-omics analyses (genomics, transcriptomics, proteomics and metabolomics), to reveal the synergistic roles of cordyceps components in treating neurological disorders and identify potential molecular biomarkers. Additionally, we highlight the findings from preclinical experiments and animal models on cordyceps-based drugs, discussing their advantages and challenges for clinical application. Future studies should prioritize systematic exploration of standardized drug development, advanced multi-omics integration, and rigorous clinical trials. This will provide a more robust scientific foundation and practical guidance for the treatment of neurological diseases with cordyceps.</p>\u0000 </div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"113 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147626856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Widespread distribution of honey bee-associated pathogens in managed and wild bees","authors":"Nuno Capela,&nbsp;Rossella Tiritelli,&nbsp;Artur Sarmento,&nbsp;Soraia Sousa Santos,&nbsp;Giovanni Cilia","doi":"10.1007/s00114-026-02094-3","DOIUrl":"10.1007/s00114-026-02094-3","url":null,"abstract":"<div>\u0000 \u0000 <p>Transmission of pathogens from managed to wild bees presents a significant ecological threat in diverse habitats. Interspecific pathogen transmission is widely documented and occurs frequently in shared environments. This study examines the prevalence and abundance of the bee-associated pathogens (DWV, BQCV, ABPV, CBPV, KBV, SBV, <i>Nosema ceranae</i>, <i>Crithidia mellificae</i>, <i>Lotmaria passim</i>, and <i>Crithidia bombi</i>) in wild bees sampled in sites with and without honey bees in the Coimbra district (Portugal). Using quantitative PCR and statistical modelling, the study identified interspecific pathogen transmission dynamics influenced by the presence of <i>Apis mellifera</i> colonies and wild bee genera. The findings revealed a high prevalence of pathogens, with DWV (87.22%), CBPV (70.71%), ABPV (48.95%) and <i>N. ceranae</i> (30.03%) being the most common in species. There is a broad susceptibility of different genera of bees, with a higher abundance of pathogens in <i>Andrena</i>, <i>Halictus</i>, and <i>Megachile</i>. The risk of ABPV and SBV infections was associated with sites close to the apiaries. Furthermore, ABPV and <i>L. passim</i> were mainly associated with honey bees, while <i>C. bombi</i> was associated with bumblebees. This study represents the first investigation of wild bee pathogens in mainland Portugal. It emphasizes the urgent need for integrative conservation strategies under a One-Health approach to mitigate the impacts of pathogens on pollinator biodiversity and ecosystem stability.</p>\u0000 </div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"113 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147588580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of flight loss in Island endemic stag beetle Prosopocoilus hachijoensis (Coleoptera: Lucanidae): a comparative analysis of flight and walking traits among congeners","authors":"Tai Komoriya,&nbsp;Tatsuya Saga,&nbsp;Mito Ikemoto,&nbsp;Tomoyuki Yokoi","doi":"10.1007/s00114-026-02081-8","DOIUrl":"10.1007/s00114-026-02081-8","url":null,"abstract":"<div>\u0000 \u0000 <p>Flight is a crucial locomotion strategy in insects, facilitating predator avoidance, foraging, and mating. However, when its energetic costs outweigh the benefits, flight capabilities can be lost secondarily. Flight loss has been studied in species exhibiting intraspecific polymorphisms, whereas both proximate and ultimate factors remain poorly understood in monomorphically flightless species. This study investigates the mechanisms of flight loss in <i>Prosopocoilus hachijoensis</i>, a flightless stag beetle, through comparison with flight-capable <i>Prosopocoilus</i> species. We hypothesized that flight muscle atrophy is a proximate factor, whereas reduced dependence on flight and increased reliance on terrestrial locomotion are the ultimate factors. We conducted field sampling in the Izu Islands, Japan, to examine morphological and behavioral traits related to both flight and walking. All species attempted flight, including the flightless <i>P. hachijoensis</i>. Wing reduction was observed in <i>P. hachijoensis</i> females and flight muscle atrophy was exclusive to this species. Allometric analyses revealed that <i>P. hachijoensis</i> exhibits reduced relative wing size compared with flight-capable congeners. While both male and female <i>P. hachijoensis</i> exhibited a smaller ratio of tarsal length to total leg length, the males’ walking speed did not differ from their congeners. In contrast, females of <i>P. hachijoensis</i> walked faster and showed a lower immobility frequency. We concluded that flight muscle atrophy is a proximate factor for flight loss in <i>P. hachijoensis</i>, and increased reliance on walking as locomotion may represent a potential ultimate factor underlying flight loss.</p>\u0000 </div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"113 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in silico evaluation of copper nanoparticle-phytoconjugates as multi-target antidiabetic agents","authors":"R. Meenakshi,&nbsp;S. Sudharson,&nbsp;R. Subashini,&nbsp;S. Abirami,&nbsp;MD Tameem Tabbasum","doi":"10.1007/s00114-026-02084-5","DOIUrl":"10.1007/s00114-026-02084-5","url":null,"abstract":"<div><p>Type 2 diabetes mellitus (T2DM) is characterized by elevated blood glucose levels and insulin resistance. Trigonella foenum-graecum (fenugreek) has been extensively reported for its hypoglycemic and insulin-sensitizing properties mediated through multiple biochemical pathways. This study aimed to synthesize copper nanoparticles (CuNPs) using fenugreek seed extract and the investigation of their antidiabetic activity through an integrated in vitro and in silico approach. Qualitative phytochemical analysis confirmed the presence of secondary metabolites. The in vitro enzyme inhibition assays revealed promising suppression of digestive enzymes: α-amylase (88.72%) and α-glucosidase (91.02%) at 500 µg/mL, indicating a postprandial glucose-lowering capacity. UV-Vis spectroscopy indicated a distinct surface plasmon resonance peak at 546 nm while XRD and FTIR analyses respectively confirmed the crystalline nature and the functional group that were involved in CuNP stabilization. Network pharmacology and molecular docking studies showed significant interactions between the CuNP-ligand conjugates and the diabetes-associated target genes. Moreover, ADMET analysis of the ligands, thymol and anthranilic acid, indicated good oral bioavailability. Overall, the findings demonstrate in vitro antidiabetic activity, while the proposed molecular targets and pathways derived from the network pharmacology and molecular docking analyses were employed as computational, hypotheses-generating approaches and the mechanisms have not been experimentally validated. This integrated phyto-nanoparticle strategy represents a promising cost-effective and sustainable nanotherapeutic approach for diabetes management.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"113 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed priming to induce seed germination, seedling growth and enzymatic activity in watermelon","authors":"Zarir Sharaf,&nbsp;Nasratullah Habibi,&nbsp;Amruddin Fakhri,&nbsp;Shah Mahmoud Faqiri","doi":"10.1007/s00114-026-02100-8","DOIUrl":"10.1007/s00114-026-02100-8","url":null,"abstract":"<div>\u0000 \u0000 <p>Poor and non-uniform germination, low seedling vigor, and high susceptibility to seed-borne infections constrain successful establishment of watermelon (<i>Citrullus lanatus</i> L.) crops. Seed hydro-priming is a simple and cost-effective pre-sowing technique that enhances seed physiological quality by initiating metabolic processes prior to germination. This study evaluated the effects of seed hydro-priming under different soaking temperatures and post-priming drying conditions on germination, seedling growth, vigor indices, physiological and biochemical attributes, seed health, and field emergence of watermelon cv. Chandraprabhavati. Seeds were hydro-primed in distilled water (1:2, w/v) for 18 h at ambient, 10, 25, and 27 °C, followed by drying under control, shade, 30, 35, and 40 °C conditions in a Completely Randomized Design with three replications. Hydro-priming significantly improved germination, seedling growth, vigor indices, and field emergence compared with non-primed seeds. Germination increased from 75% in control to 96% under, while seedling dry weight increased to 36.92% under seed priming treatment. Electrical conductivity declined markedly, whereas seed leachate pH and total dehydrogenase activity increased, indicating enhanced membrane stability and respiratory metabolism. Seed hydro-priming enhanced seed tolerance to post priming infection from 70% to 5%. Overall, hydro-priming at 25–27 °C followed by drying at 30–35 °C is an effective, low-cost strategy to improve watermelon seed quality and field performance.</p>\u0000 </div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"113 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00114-026-02100-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corilagin induces apoptosis in glioblastoma cells by disrupting metabolic homeostasis: a metabolomics perspective","authors":"Fei Liu,&nbsp;Miaomiao Zhang,&nbsp;Qipeng Long,&nbsp;Yinuo Xiao,&nbsp;Qunfei Ma,&nbsp;Bing Han,&nbsp;Yuzhen Gao,&nbsp;Zhiqiang Liang,&nbsp;Yan Lu","doi":"10.1007/s00114-026-02097-0","DOIUrl":"10.1007/s00114-026-02097-0","url":null,"abstract":"<div>\u0000 \u0000 <p>Glioblastoma (GBM) is a highly aggressive brain tumor with limited therapeutic potions. Corilagin, a natural polyphenol, exhibits anti-tumor activity, but its systemic metabolic mechanisms in GBM remain uncharacterized. We treated human glioblastoma LN229 and T98G cells with corilagin and evaluated its effects on cell viability, apoptosis, and migration. An untargeted liquid chromatography-mass spectrometry (LC-MS)-based metabolomics approach was performed to comprehensively profile global metabolic alterations in LN229 cells, followed by multivariate statistical analysis and KEGG pathway enrichment. Corilagin significantly inhibited proliferation, induced apoptotic cell death, and suppressed cell migration in a dose-dependent manner in LN229 and T98G cells. Moreover, principal component analysis and partial least squares-discriminate analysis clearly demonstrated that corilagin disrupted the cell metabolic profiles. Forty differential metabolites in LN229 cells responsible for the intervention effect of corilagin were screened and annotated. These metabolites corresponded to thirteen metabolic pathways. Integrated pathway analysis identified profound disruptions in nucleotide metabolism (notably purine and pyrimidine depletion), glycerophospholipid homeostasis (reduced phosphatidylcholine and phosphatidylethanolamine species), and the TCA cycle. The multi-pathway metabolic collapse was associated with the induction of apoptosis. Our study demonstrates that corilagin exerts its potent anti-glioma effects by inducing a coordinated disruption of core metabolic networks essential for GBM cell survival. These findings establish a strong association between corilagin-induced metabolic reprogramming and apoptotic cell death, highlighting its promise as a therapeutic agent targeting the metabolic vulnerabilities of glioblastoma.</p>\u0000 </div>","PeriodicalId":794,"journal":{"name":"The Science of Nature","volume":"113 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00114-026-02097-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}