Cytokines, cellular & molecular therapy最新文献_第9页

Dysregulation of inflammatory cytokines in unrelated bone marrow transplantation. 非亲属骨髓移植中炎症细胞因子的失调。

Cytokines, cellular & molecular therapy Pub Date : 1998-09-01

A Nagler, A Bishara, C Brautbar, V Barak

引用次数: 0

Expression of the human GM-CSF receptor alpha subunit in Saccharomyces cerevisiae. 人GM-CSF受体α亚基在酿酒酵母中的表达。

Cytokines, cellular & molecular therapy Pub Date : 1998-09-01

J Tu, D W Golde, J C Vera, M L Heaney

引用次数: 0

Macrophage activity, IL-6 levels, antibody response and heart histology in rats undergoing an attenuated Trypanosoma cruzi acute infection upon treatment with recombinant interferon gamma. 重组干扰素γ治疗克氏锥虫急性减毒感染大鼠的巨噬细胞活性、IL-6水平、抗体反应和心脏组织学

Cytokines, cellular & molecular therapy Pub Date : 1998-09-01

S Revelli, G Didoli, E Roggero, H Moreno, J Bernabo, J Wietzerbin, O Bottasso

{"title":"Macrophage activity, IL-6 levels, antibody response and heart histology in rats undergoing an attenuated Trypanosoma cruzi acute infection upon treatment with recombinant interferon gamma.","authors":"S Revelli, G Didoli, E Roggero, H Moreno, J Bernabo, J Wietzerbin, O Bottasso","doi":"","DOIUrl":"","url":null,"abstract":"Earlier experiments in Trypanosoma cruzi-infected rats showed that recombinant rat (Rr) interferon (IFN)-gamma given shortly after infection ameliorated acute disease without modifying the serum titers of endogenously synthesized IFN-gamma and tumor necrosis factor. To gain some insight into the processes underlying this protective effect, 21-day old 'I' rats that were infected with T. cruzi and the following day started with a 20-day cycle of RrIFN-gamma injections (20000 IU/rat/day) were investigated for the in vitro replication of T. cruzi and nitric oxide (NO) production by peritoneal macrophages (day 7 post-infection, pi), antibodies with lytic activity against T. cruzi (days 7, 20, and 28 pi), and serum levels of biologically active interleukin (IL)-6 (days 15 and 30 pi). Therapy with RrIFN-gamma rendered cultured peritoneal macrophages less permissive to infection with T. cruzi. Such an effect was not accompanied by higher amounts of NO in macrophage cultured supernatants, compared with those from T. cruzi-infected rats receiving no RrIFN-gamma, which appeared not to be protected from in vitro infection. Acutely T. cruzi-infected rats had significant amounts of IL-6 in their sera - this not being the case in infected rats given RrIFN-gamma, whose levels appeared decreased as in control rats. The presence of complement-mediated anti-T. cruzi lytic antibodies was not modified by RrIFN-gamma. Likewise, heart histology at day 7 pi revealed that treatment with RrIFN-gamma made no differences as to the amount of acute inflammation, but tended to reduce the myocardial parasite load.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"4 3","pages":"153-9"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20737336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The implications of granulocyte-monocyte colony-stimulating factor (GM-CSF) in cytotoxicity of bone marrow transplantation. 粒细胞-单核细胞集落刺激因子(GM-CSF)在骨髓移植细胞毒性中的意义。

Cytokines, cellular & molecular therapy Pub Date : 1998-09-01

A Toren, A Nagler

引用次数: 0

Role of cytokines in the maintenance of the memory immune response induced by a rat histiocytoma in syngeneic hosts. 细胞因子在同基因宿主大鼠组织细胞瘤诱导的记忆免疫反应维持中的作用。

Cytokines, cellular & molecular therapy Pub Date : 1998-09-01

K S Nandakumar, C Varalakshmi, A Khar

{"title":"Role of cytokines in the maintenance of the memory immune response induced by a rat histiocytoma in syngeneic hosts.","authors":"K S Nandakumar, C Varalakshmi, A Khar","doi":"","DOIUrl":"","url":null,"abstract":"The nature of immune memory induced by a rat histiocytoma, AK-5, in syngeneic hosts was studied. AK-5 tumor when transplanted intraperitoneally (i.p.) into naive animals grows as ascites and is 100% fatal. However, spontaneous regression of AK-5 tumor was observed in 60% of animals upon s.c. transplantation. Interestingly, all the tumor-rejected animals (immune) were found to resist further i.p. challenges with AK-5 cells. The immunity thus developed is specific for AK-5 tumor, since other tumors grow in these animals. In order to understand the tumor-specific immune memory induced after AK-5 tumor transplantation, we have evaluated circulatory-cytokine profiles of i.p. tumor-transplanted naive and immune animals. Our results show an increase in the levels of IL-2, IL-12 and IL-4 in tumor-injected immune animals compared with normal animals, whereas the interferon-gamma levels were totally reversed in these two sets of animals. We also found elevated levels of circulating immune complexes in the sera from AK-5-rechallenged immune animals. We have also evaluated the cytotoxic potential of splenocytes and pure natural killer cells from immune animals rechallenged with AK-5 cells, and have found a significant increase in antibody-dependent cellular cytotoxicity. Similarly, in vitro proliferation of total splenocytes and nylon-wool non-adherent cells from immune animals was much higher compared with the normal animals. The present study thus suggests antigen-independent maintenance of clonal burst size, which could be the form of immune memory induced by AK-5 tumor in the syngeneic host.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"4 3","pages":"169-75"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20737338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Techniques for quantification of cytokine mRNAs. 细胞因子mrna的定量技术。

Cytokines, cellular & molecular therapy Pub Date : 1998-09-01

O Benveniste, M Martin, F Villinger, D Dormont

引用次数: 0

G-CSF production in human bladder cancer and its ability to promote autocrine growth: a review. 人类膀胱癌中G-CSF的产生及其促进自分泌生长的能力:综述。

Cytokines, cellular & molecular therapy Pub Date : 1998-06-01

M Tachibana, M Murai

{"title":"G-CSF production in human bladder cancer and its ability to promote autocrine growth: a review.","authors":"M Tachibana, M Murai","doi":"","DOIUrl":"","url":null,"abstract":"A variety of non-hematopoietic malignant tumors have been demonstrated to secrete granulocyte colony-stimulating factor (G-CSF) in amounts large enough to cause a significant systemic hematopoietic effect. Meanwhile, bladder cancer cells have been shown to secrete a variety of biological factors with no direct relation to urothelial cell origin. G-CSF produced by non-hematopoietic malignant cells in particular has been reported to be capable of inducing a leukemoid reaction in the host through intense stimulation of leukocyte production. This is most frequently associated with aggressive tumor cell growth and a poor clinical outcome. On the other hand, receptors for G-CSF have also been found on the cell surfaces of several non-hematopoietic cell types. These observations lead naturally to the tempting speculation that simultaneous acquisition of the ligand promotion and its receptor expression by a malignant tumor may provide a strong autocrine growth advantage. However, the role of autocrine growth factors in malignancy is even less clear, although it is undoubtedly important. In this review, G-CSF and tumor cell growth, particularly of human transitional cell carcinomas of the bladder, are discussed, and autocrine growth of human solid tumors is also summarized.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"4 2","pages":"113-20"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20597933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytokine gene transduction into non-immunogeneic murine tumor cells. 细胞因子基因转导到非免疫基因小鼠肿瘤细胞。

Cytokines, cellular & molecular therapy Pub Date : 1998-06-01

S Morecki, A Lubina-Salomon, S Slavin, A Nagler

{"title":"Cytokine gene transduction into non-immunogeneic murine tumor cells.","authors":"S Morecki, A Lubina-Salomon, S Slavin, A Nagler","doi":"","DOIUrl":"","url":null,"abstract":"The effect of cytokine transduction on the tumorigenicity and immunogenicity of murine non-immunogeneic mammary carcinoma (4T1), acute myeloid leukemia (mAML) and partially immunogenic B-cell leukemia (BCL1) has been evaluated in syngeneic strains of mice. Transduction by retroviral vectors containing the genes for GM-CSF, IL-2 or IFN-gamma did not lead to a marked antitumor effect in 4T1 mammary tumor or BCL1. A reduced local tumor size was observed in mice inoculated with 4T1 cells transduced with both GM-CSF and IL-2 genes followed by an in vitro exposure to recombinant IFN-gamma, but survival was not prolonged. Tumorigenicity of mAML cells transduced with the gene coding for IFN-gamma was significantly reduced as manifested by prolonged survival of mice in comparison with animals inoculated with non-transduced mAML cells. Transduction by each of the aforementioned cytokines did not affect the immunogenicity of these tumor model cells. The results suggest that genetic modification of spontaneous and non-immunogenic experimental tumor models does not necessarily support direct utilization of cytokine gene therapy for clinical application. More effective methods have yet to be established in order to achieve an antitumor effect in spontaneous non-immunogenic malignancies.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"4 2","pages":"87-94"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20598599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

G-CSF production in human bladder cancer and its ability to promote autocrine growth: a review. 人类膀胱癌中G-CSF的产生及其促进自分泌生长的能力:综述。

Cytokines, cellular & molecular therapy Pub Date : 1998-06-01 DOI: 10.1016/S0022-5347(05)68641-7

M. Tachibana, M. Murai

引用次数: 35

Intraperitoneal immunotherapy of peritoneal carcinomatosis. 腹膜癌的腹腔免疫治疗。

Cytokines, cellular & molecular therapy Pub Date : 1998-06-01

R S Freedman, R Lenzi, A P Kudelka, D D Lawrence, M Rosenblum, C D Platsoucas

{"title":"Intraperitoneal immunotherapy of peritoneal carcinomatosis.","authors":"R S Freedman, R Lenzi, A P Kudelka, D D Lawrence, M Rosenblum, C D Platsoucas","doi":"","DOIUrl":"","url":null,"abstract":"Carcinomas that originate in the ovary or from different sites in the gastrointestinal tract frequently develop metastases that involve the peritoneal and serosal surfaces. Carcinomatous involvement of the peritoneum is a significant cause of morbidity and mortality. Advances in our understanding of the immunobiology of the peritoneal cavity and the availability of technically advanced immunotherapeutic agents are providing an important opportunity for the intraperitoneal delivery of these agents. This review describes newer concepts in tumor immunology that have a bearing on the further development of intraperitoneal immunotherapy; delivery systems for and issues to be resolved in intraperitoneal immunotherapy, and results of studies with recombinant interferons alpha and gamma and interleukin (IL)-2, cellular therapies including lymphokine-activated killer cells, tumor-infiltrating lymphocytes, monoclonal antibodies, and intraperitoneal radioimmunotherapy. New trials of intraperitoneal immunotherapy employing novel agents, including IL-12 and genetically modified tumor vaccines, are discussed, as are issues related to the integration of immunotherapy with standard chemotherapy agents. A number of immunotherapy agents have been tested intraperitoneally and have shown promising clinical activity with acceptable toxicity. Complete responses have been documented at surgical restaging, and intraperitoneal treatments with these agents may soon be included in the therapeutic armamentarium for patients with peritoneal carcinomatosis.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"4 2","pages":"121-40"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20597934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0