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CONTENTS OF BRAIN RESEARCH PROTOCOLS VOLUME 16 脑研究协议第16卷的内容

Brain research. Brain research protocols Pub Date : 2005-12-01 DOI: 10.1016/S1385-299X(05)00099-1

引用次数: 0

Free colour illustrations in the online version of articles 免费彩色插图在文章的在线版本

Brain research. Brain research protocols Pub Date : 2005-12-01 DOI: 10.1016/S1385-299X(05)00097-8

引用次数: 0

Free colour illustrations on the web 免费彩色插图在网络上

Brain research. Brain research protocols Pub Date : 2005-09-01 DOI: 10.1016/S1385-299X(05)00073-5

引用次数: 0

High-pressure freezing followed by cryosubstitution as a tool for preserving high-quality ultrastructure and immunoreactivity in the Xenopus laevis pituitary gland 高压冷冻后冷冻替代作为保存非洲爪蟾脑垂体高质量超微结构和免疫反应性的工具

Brain research. Brain research protocols Pub Date : 2005-09-01 DOI: 10.1016/j.brainresprot.2005.07.001

Liangchun Wang , Bruno M. Humbel , Eric W. Roubos

{"title":"High-pressure freezing followed by cryosubstitution as a tool for preserving high-quality ultrastructure and immunoreactivity in the Xenopus laevis pituitary gland","authors":"Liangchun Wang , Bruno M. Humbel , Eric W. Roubos","doi":"10.1016/j.brainresprot.2005.07.001","DOIUrl":"10.1016/j.brainresprot.2005.07.001","url":null,"abstract":"<div><p>Subcellular localisation of proteins and peptides yields fundamental information about cell functioning. Immunoelectron microscopy is a powerful tool to achieve this goal, but combining good tissue preservation with strong immunoreactivity is a great challenge in electron microscopy. We have applied a novel approach, using high-pressure freezing (HPF) followed by cryosubstitution, to prepare the pituitary gland of the amphibian <em>Xenopus laevis</em> for immunogold-electron microscopy. In this way, we investigated the subcellular distribution of brain-derived neurotrophic factor and the amphibian neurohormone mesotocin in the pituitary neural lobe, and the peptide hormone α-melanophore-stimulating hormone and its protein precursor proopiomelanocortin in melanotrope cells of the pituitary intermediate lobe. In contrast to conventional chemical fixation (followed by cryosubstitution), HPF not only revealed strong immunoreactivity of the secretory products, but also provided excellent ultrastructural preservation of cell organelles, including secretory granule subtypes. We conclude that HPF followed by cryosubstitution provides a preparation technique of choice when both optimal tissue ultrastructure and strong immunoreactivity are required.</p></div>","PeriodicalId":79477,"journal":{"name":"Brain research. Brain research protocols","volume":"15 3","pages":"Pages 155-163"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.brainresprot.2005.07.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25258642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Oxidation of 14C-labeled substrates by hippocampal slice cultures 海马切片培养对14c标记底物的氧化作用

Brain research. Brain research protocols Pub Date : 2005-09-01 DOI: 10.1016/j.brainresprot.2005.06.002

Erin Gilbert , Peter J. Bergold

引用次数: 5

Peripheral targeting of the trigeminal ganglion via the infraorbital foramen as a therapeutic strategy 经眶下孔外周靶向三叉神经节作为一种治疗策略

Brain research. Brain research protocols Pub Date : 2005-09-01 DOI: 10.1016/j.brainresprot.2005.05.003

John K. Neubert , Andrew J. Mannes , Jason Keller , Melanie Wexel , Michael J. Iadarola , Robert M. Caudle

{"title":"Peripheral targeting of the trigeminal ganglion via the infraorbital foramen as a therapeutic strategy","authors":"John K. Neubert , Andrew J. Mannes , Jason Keller , Melanie Wexel , Michael J. Iadarola , Robert M. Caudle","doi":"10.1016/j.brainresprot.2005.05.003","DOIUrl":"10.1016/j.brainresprot.2005.05.003","url":null,"abstract":"<div><p><span><span><span>A unique interventional approach for modulating sensory signaling involves targeting neurons in the sensory ganglia through use of pharmacological or gene therapies. This has previously been accomplished in the trigeminal system via stereotactic ganglionic microinjections. However, this procedure can be time consuming and requires expensive stereotactic equipment. The current paper describes a percutaneous approach for injecting the trigeminal ganglion with an electrical-stimulation needle inserted through the infraorbital foramen, </span>infraorbital canal and </span>foramen rotundum<span>. The needle tip was positioned in the ganglion by eliciting a twitch in the ipsilateral masseter following electrical stimulation. The technique was imaged using computerized tomography<span> and methylene blue dye to verify the site of the injection. We validated this technique by reproducing the results from our prior study that involved injection of </span></span></span>resiniferatoxin (RTX) into the trigeminal ganglion using the stereotactic approach. Both techniques for intraganglionic injection of RTX produced a specific deletion of C-fiber neurons, as demonstrated by inhibition of neurogenic-induced plasma extravasation and behavioral assays. Thus, we propose a novel and simple technique for studying mechanisms of peripheral sensory modulation of orofacial pain via direct application of drugs, tracers or viral vectors around trigeminal sensory neuronal cell bodies. This technique minimizes trauma to brain structures that may have an impact on pain perception.</p></div>","PeriodicalId":79477,"journal":{"name":"Brain research. Brain research protocols","volume":"15 3","pages":"Pages 119-126"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.brainresprot.2005.05.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25194722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 52

Proliferation markers in the adult rodent brain: Bromodeoxyuridine and proliferating cell nuclear antigen 成年啮齿动物脑中的增殖标志物:溴脱氧尿苷和增殖细胞核抗原

Brain research. Brain research protocols Pub Date : 2005-09-01 DOI: 10.1016/j.brainresprot.2005.06.001

Jorge Valero, Eduardo Weruaga, Azucena R. Murias, Javier S. Recio, José R. Alonso

{"title":"Proliferation markers in the adult rodent brain: Bromodeoxyuridine and proliferating cell nuclear antigen","authors":"Jorge Valero, Eduardo Weruaga, Azucena R. Murias, Javier S. Recio, José R. Alonso","doi":"10.1016/j.brainresprot.2005.06.001","DOIUrl":"10.1016/j.brainresprot.2005.06.001","url":null,"abstract":"<div><p>The rostral migratory stream<span><span><span> is one of the few regions of the adult mammalian central nervous system<span> in which cellular migration and proliferation have been described. Most rostral migratory stream cells divide rapidly and hence different proliferation markers have been employed to identify them. Nitrogen base substitutes, such as tritiated thymidine or 5-bromo-2′-deoxyuridine (BrdU), together with endogenous molecules, such as </span></span>Proliferating Cell Nuclear Antigen (PCNA), are the cell cycle markers most widely employed. Protocols for </span>BrdU<span> and PCNA localization are both plentiful and diverse, but to date no optimized protocol for obtaining trustworthy double staining of both markers has been described. In this work, we propose optimized protocols for achieving both single staining and the joint detection of BrdU and PCNA in the rodent brain using double-immunofluorescence procedures. The double labeling described allows the discrimination of different cell cycle stages in migratory cells from the mouse brain.</span></span></p></div>","PeriodicalId":79477,"journal":{"name":"Brain research. Brain research protocols","volume":"15 3","pages":"Pages 127-134"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.brainresprot.2005.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25194724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Reanalysis of the protocol for in vitro synchronization of mammalian astrocytic cultures by serum deprivation 哺乳动物星形细胞血清剥夺体外同步培养方案的再分析

Brain research. Brain research protocols Pub Date : 2005-09-01 DOI: 10.1016/j.brainresprot.2005.05.002

Stephen Cooper

引用次数: 10

Regression analysis utilizing subjective evaluation of emotional experience in PET studies on emotions 利用情绪体验主观评价的PET情绪研究回归分析

Brain research. Brain research protocols Pub Date : 2005-09-01 DOI: 10.1016/j.brainresprot.2005.06.003

Sargo Aalto , Esa Wallius , Petri Näätänen , Jaana Hiltunen , Liisa Metsähonkala , Hannu Sipilä , Hasse Karlsson

{"title":"Regression analysis utilizing subjective evaluation of emotional experience in PET studies on emotions","authors":"Sargo Aalto , Esa Wallius , Petri Näätänen , Jaana Hiltunen , Liisa Metsähonkala , Hannu Sipilä , Hasse Karlsson","doi":"10.1016/j.brainresprot.2005.06.003","DOIUrl":"10.1016/j.brainresprot.2005.06.003","url":null,"abstract":"<div><p>A methodological study on subject-specific regression analysis (SSRA) exploring the correlation between the neural response and the subjective evaluation of emotional experience in eleven healthy females is presented. The target emotions, i.e., amusement and sadness, were induced using validated film clips, regional cerebral blood flow (rCBF) was measured using positron emission tomography (PET), and the subjective intensity of the emotional experience during the PET scanning was measured using a category ratio (CR-10) scale. Reliability analysis of the rating data indicated that the subjects rated the intensity of their emotional experience fairly consistently on the CR-10 scale (Cronbach alphas 0.70–0.97). A two-phase random-effects analysis was performed to ensure the generalizability and inter-study comparability of the SSRA results. Random-effects SSRAs using Statistical non-Parametric Mapping 99 (SnPM99) showed that rCBF correlated with the self-rated intensity of the emotional experience mainly in the brain regions that were identified in the random-effects subtraction analyses using the same imaging data. Our results give preliminary evidence of a linear association between the neural responses related to amusement and sadness and the self-evaluated intensity of the emotional experience in several regions involved in the emotional response. SSRA utilizing subjective evaluation of emotional experience turned out a feasible and promising method of analysis. It allows versatile exploration of the neurobiology of emotions and the neural correlates of actual and individual emotional experience. Thus, SSRA might be able to catch the idiosyncratic aspects of the emotional response better than traditional subtraction analysis.</p></div>","PeriodicalId":79477,"journal":{"name":"Brain research. Brain research protocols","volume":"15 3","pages":"Pages 142-154"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.brainresprot.2005.06.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24982099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

CONTENTS OF BRAIN RESEARCH PROTOCOLS VOLUME 15 脑研究协议第15卷的内容

Brain research. Brain research protocols Pub Date : 2005-09-01 DOI: 10.1016/S1385-299X(05)00075-9

引用次数: 0