The Protein Journal最新文献

{"title":"GCMS and Molecular Docking Based Insights into the Antifungal Potential of Bacillus Bs-06 Against Fusarium oxysporum f. sp. lycopersici","authors":"K. Vigneshwaran,&nbsp;K. Rajamohan,&nbsp;P. Balabaskar,&nbsp;P. Sivasakthivelan,&nbsp;N. H. Shankar Reddy","doi":"10.1007/s10930-025-10306-4","DOIUrl":"10.1007/s10930-025-10306-4","url":null,"abstract":"<div><p>The utilization of <i>Bacillus</i> spp. as eco-compatible bioresources represented a promising strategy in antifungal biotechnology. In this study, <i>Bacillus subtilis</i> Bs-06, isolated from the rhizosphere of healthy tomato plants, exhibited strong antagonistic activity through the secretion of diverse bioactive metabolites. Gas chromatography–mass spectrometry (GC–MS) analysis of its culture extract identified ten major compounds, including Phenol-3,5-bis(1,1-dimethylethyl), palmitic acid, oleic acid, and octadecanoic acid, which had been widely reported for antimicrobial efficacy. To investigate their Molecular Docking targeted the key virulence proteins of <i>Fusarium oxysporum</i> f.sp. <i>lycopersici</i> Cutinase (5AJH), Avr1/SIX4 (7T6A), and Avr3/SIX1 (7T69). Docking simulations revealed strong binding affinities, with Phenol-3,5-bis(1,1-dimethylethyl) showing the highest interaction energy (− 11.53 kcal/mol) against Avr3. The ligand–protein complexes were stabilized by hydrogen bonding and hydrophobic interactions, suggesting potential inhibition of pathogen virulence functions. Normal mode analysis further demonstrated differential flexibility among protein–ligand complexes, where Avr1 exhibited the highest conformational mobility, while Cutinase displayed the greatest structural rigidity. These findings established Bs-06 as a valuable source of antifungal metabolites capable of targeting virulence determinants at the molecular level. The integration of GC–MS profiling, molecular docking, and dynamic simulations underscored the potential of <i>Bacillus</i> derived metabolites as sustainable biocontrol agents for managing Fusarium wilt in tomato.</p></div>","PeriodicalId":793,"journal":{"name":"The Protein Journal","volume":"45 1","pages":"146 - 177"},"PeriodicalIF":1.4,"publicationDate":"2025-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145477108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal Dependent Glycosidases of Sea Cucumber Eupentacta Fraudatrix and their Properties","authors":"Svetlana E. Soboleva,&nbsp;Nadejda A. Maltseva,&nbsp;Irina A. Kostrikina,&nbsp;Pavel S. Dmitrenok,&nbsp;Georgy A. Nevinsky","doi":"10.1007/s10930-025-10301-9","DOIUrl":"10.1007/s10930-025-10301-9","url":null,"abstract":"<div><p>Sea cucumbers <i> Eupentacta fraudatrix</i> can completely regenerate their organs within one month after evisceration. The regeneration process involves complex structural changes including restructuring of the intracellular matrix. The connective tissue of echinoderms consists of bundles of collagen fibrils and proteoglycans. It is assumed that animals capable of regeneration must possess a diverse set of proteases and glycosylases that modify the connective tissue. Glycosidases catalyze the hydrolysis of glycosidic bonds in carbohydrate molecules, and these enzymes have not yet been studied in the sea cucumber <i>E. fraudatrix</i>. Here four glycosidase-enriched fractions having optimal pH values at 7.0, 7.5 (two fractions), and 6.0 were revealed. Thе glycosidase of Peak 1 (pH 7.0) was moderately activated by Mn²⁺, Ca²⁺, and Zn²⁺ but its best activators are Co²⁺ and Ni²⁺, while Mg²⁺ ions suppress glycosidase activity. Thе glycosidase of Peak 2 (pH 7.5) is activated at low concentrations of Ca²⁺ and Mg²⁺; Mn²⁺ and Co²⁺ increase its activity at higher concentrations, while Ni²⁺ and Zn²⁺ are inhibitors of this enzyme. Thе glycosidase of Peak 3 (pH 7.5) has maximal activity in the presence of Ca²⁺ and Mg²⁺ ions but its activity is suppressed by Mn²+, Ni²⁺, and Zn²⁺. Acid glycosidase (pH 6.0) is Ca²⁺ dependent enzyme, which activity suppresses by several metal ions to varying degrees: Zn²⁺ &gt; Mg²⁺ &gt; Ni²⁺ &gt; Mn²⁺ &gt; Co²⁺. These are the first glycosidases identified in the sea cucumber <i>E. fraudatrix</i> that can be utilized for future studies of their role in extracellular matrix remodeling.</p></div>","PeriodicalId":793,"journal":{"name":"The Protein Journal","volume":"45 1","pages":"115 - 124"},"PeriodicalIF":1.4,"publicationDate":"2025-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145433654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylglyoxal-Induced Modification of Hen Egg White Lysozyme: Detection of Advanced Glycation End Products by High Resolution Mass Spectrometry","authors":"Sauradipta Banerjee","doi":"10.1007/s10930-025-10299-0","DOIUrl":"10.1007/s10930-025-10299-0","url":null,"abstract":"<div><p>Methylglyoxal is a highly reactive α-oxoaldehyde that forms advanced glycation end products (AGEs) on reaction with proteins. Here, we have studied the effect of methylglyoxal on hen egg white lysozyme (HEWL), after incubation for different time periods (7, 14 and 21 days). Modification with methylglyoxal induced a gradual lowering of tryptophan fluorescence of the protein associated with a blue shift in the wavelength of fluorescence maximum intensity, as observed from tryptophan fluorescence spectra. Secondary structural analysis by far-UV CD spectroscopy indicated a gradual increase in α-helical content of the protein following incubation with methylglyoxal for different time periods. Subsequent analysis of methylglyoxal incubated protein samples using high-resolution ESI–MS, indicated modification of HEWL and formation of AGE adducts. HEWL incubated with methylglyoxal for 7 days indicated the formation of the AGE hydroimidazolone. Several AGE adducts, namely, hydroimidazolone, argpyrimidine, tetrahydropyrimidine, carboxymethyllysine and pyrrolidone-carboxymethyllyine were identified for HEWL incubated with methylglyoxal for 14 days. Thus, the extent of AGE formation was found to increase with increasing period of incubation with the α-oxoaldehyde as revealed by mass spectral analysis. As indicated in further studies, methylglyoxal modification was found to afford considerable resistance to the protein against stress induced aggregation. Considering the high reactivity of the α-dicarbonyl compound, the current study appears worthwhile in terms of detection of methylglyoxal-derived AGE adducts as well as understanding AGE induced protein modifications with clinical implications in treating AGE related disorders.</p></div>","PeriodicalId":793,"journal":{"name":"The Protein Journal","volume":"44 6","pages":"717 - 728"},"PeriodicalIF":1.4,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145350751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular, Structural and Functional Characterization of Antheraea mylitta Expressed Cocoonase: An Integrative Approach","authors":"Nishi Prakash Tiwari,&nbsp;Jay Prakash Pandey,&nbsp;Dev Mani Pandey","doi":"10.1007/s10930-025-10294-5","DOIUrl":"10.1007/s10930-025-10294-5","url":null,"abstract":"<div><p>Cocoonase, a vital naturally secreted protease, enables moth emergence by specifically degumming sericin from cocoons, leaving fibroin intact. This unique characteristic makes it highly promising for protein-based silk degumming. Present study comprehensively characterizes molecular, structural, and functional attributes of <i>Antheraea mylitta</i> cocoonase. Here 786 bp full-length cocoonase gene from <i>A. mylitta</i> pupae head RNA has been successfully cloned and expressed in <i>E. coli</i> and sequence obtained deposited in NCBI (Accession ID: ON261685). Extensive <i>in-silico</i> analyses including 3D structure prediction, motif analysis, phylogenetic relationships, TMHMM, InterProScan, STRING-based protein interactions, and flexibility predictions provided deeper structural insights. Gene ontology classified cocoonase as a serine-type endopeptidase. <i>A. mylitta</i> cocoonase protein of 261-amino acid exhibited a theoretical molecular weight of 27,663.35 Da and an isoelectric point of 9.10 while molecular weight of ~ 28 kDa by SDS-PAGE. Its presumed extracellular localization was supported by the absence of signal peptides and transmembrane helices. STRING analysis revealed functional interacting networks, while ProtScale elucidated protein flexibility. Optical Coherence Tomography based study demonstrated distinct morphological changes in cocoon sheets treated with recombinant cocoonase. This study represents the first successful cloning, expression, and comprehensive <i>in-silico</i> characterization of the <i>A. mylitta</i> cocoonase gene.</p></div>","PeriodicalId":793,"journal":{"name":"The Protein Journal","volume":"44 6","pages":"789 - 807"},"PeriodicalIF":1.4,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145350828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining Cryo-EM with Computational Approaches To Revolutionize Structural Biology","authors":"Han-ul Kim,&nbsp;Mi Young An,&nbsp;Young Kwan Kim,&nbsp;Jeong Min Chung,&nbsp;Hyun Suk Jung","doi":"10.1007/s10930-025-10296-3","DOIUrl":"10.1007/s10930-025-10296-3","url":null,"abstract":"<div><p>The development of cryo-electron microscopy (cryo-EM) has led to significant advancements in the field of structural biology. Specifically, improvements in both hardware and software for cryo-EM have not only addressed previously perceived limitations but have also enabled the accumulation of a vast amount of structural data. The integration of cryo-EM with computational methods, such as molecular docking and molecular dynamics (MD) simulations, has further advanced the study of the molecular mechanisms underlying biological processes. This review explores the transformative contributions of cryo-EM to structural biology and highlights how molecular docking and MD simulations complement experimental data to investigate protein-protein interactions. We discuss the combined application of cryo-EM, molecular docking, and MD simulations, focusing on their roles in advancing structural analysis. Finally, we consider prospective applications, emphasizing the significant influence these techniques on the structural biology. These integrated methodologies provide valuable insights into biomolecular interactions and support structure- and fragment-based drug discovery, offering more accurate and de-tailed structural characterization.</p></div>","PeriodicalId":793,"journal":{"name":"The Protein Journal","volume":"44 6","pages":"675 - 690"},"PeriodicalIF":1.4,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional and Structural Insights into Phenoloxidase from Hermetia illucens: A Combined Biochemical and Computational Approach","authors":"K. Sruthi,&nbsp;B. N. Marieshwari,&nbsp;S. Bhuvaragavan,&nbsp;S. Janarthanan","doi":"10.1007/s10930-025-10298-1","DOIUrl":"10.1007/s10930-025-10298-1","url":null,"abstract":"<div><p>The enzyme phenoloxidase (PO) is an essential component in the immune system of insects, which is responsible for the rapid activation and encapsulation of microbial pathogens. This study focuses on the in vitro and in silico investigation of PO using 4th instar larvae of black soldier fly (BSF), <i>Hermetia illucens</i>. The preliminary assays confirmed the occurrence of PO in the <i>H. illucens</i> larval haemolymph with the highest affinity towards DL-dopa as the substrate within 5 min at a λmax of 480 nm. The relative quantification of the <i>H. illucens</i> PO (HiPO) gene in the larvae showed higher expression in the fat body (FB) compared to haemocyte lysate supernatant (HLS). The PO studied under control and stress-induced conditions showed decreased activity during starvation and increased activity during injury. In silico analysis using molecular docking of various substrates with the reference structure of PO showed the highest affinity towards DL-dopa and L-dopa, followed by tyrosine, dopamine, and catechol, respectively. Hemocyanin and tyrosinase as functional domains were observed in the amino acid sequence of HiPO with an N-glycosylation site. Molecular docking of HiPO revealed a strong affinity for DL-dopa, and molecular dynamics simulation studies for the HiPO-DL-dopa complex provided their functional insights. The findings offer biomedical applications by providing an insight into the innate immune mechanisms that can guide the development of novel antimicrobial, antioxidant, and immunomodulatory strategies. This would help to comprehend the relation between immunity and metabolism, positioning <i>H. illucens</i> as a resilient feed source that supports livestock health, maintaining food safety and quality.</p></div>","PeriodicalId":793,"journal":{"name":"The Protein Journal","volume":"44 6","pages":"808 - 823"},"PeriodicalIF":1.4,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the Therapeutic Potential of Cyclic Peptide-Based Nanocarriers for Enhanced Colon Cancer Treatment","authors":"Vedhapriya Souresh,&nbsp;M. Srikayalsamyukktha,&nbsp;M. Harini,&nbsp;Yudhesh Saravanan,&nbsp;Rupachandra Saravanakumar","doi":"10.1007/s10930-025-10289-2","DOIUrl":"10.1007/s10930-025-10289-2","url":null,"abstract":"<div><p>Cyclic peptides have emerged as therapeutic agents for colon cancer due to their structural stability, enhanced bioavailability, and high target specificity. Natural cyclic peptides derived from plant, marine, and microbial sources exhibit potent anticancer properties. Advancements in nanotechnology have facilitated using cyclic peptide-based nanocarriers to improve drug delivery, enhance tumor penetration, and minimize adverse effects. Nanocarriers including liposomes, niosomes, nanosponges, and nanopolymers provide a revolutionary approach by facilitating localized distribution, enhanced stability, and controlled release of cyclic peptides. This strategy improves pharmacokinetics and lowers systemic toxicity to overcome the limitations of conventional treatments. Recent developments in cyclic peptide-based nanotechnology demonstrate the synergy between cyclic peptides and nanocarriers in overcoming drug resistance in colon cancer. This review provides a comprehensive overview of the sources, mechanisms of action, and therapeutic applications of cyclic peptides in colon cancer treatment. It further explores the role of cyclic peptide-functionalized nanocarriers in overcoming drug resistance and improving drug delivery.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><img></picture></div><div><p>Schematic representation of cyclic peptide delivery using nanocarrier systems for colon cancer treatment</p></div></div></figure></div></div>","PeriodicalId":793,"journal":{"name":"The Protein Journal","volume":"44 6","pages":"691 - 716"},"PeriodicalIF":1.4,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic Analysis of Serum Samples in Ovulatory Polycystic Ovary Syndrome and Construction of a Protein Interaction Pathway","authors":"Ahmad Fazilat,&nbsp;Keivan Majidzadeh-A,&nbsp;Kambiz Gilany,&nbsp;Fatemeh Mortazavi Moghadam,&nbsp;Mohammad Valilo,&nbsp;Saima Wajid","doi":"10.1007/s10930-025-10295-4","DOIUrl":"10.1007/s10930-025-10295-4","url":null,"abstract":"<div><p>As a heterogeneous multifactorial disorder, PCOS still has a misty etiology. Its underlying pathophysiological causes can be further elucidated by proteomic analyses and molecular network analysis to understand the interaction pathways involved in the PCOS-associated perturbations. We conducted a proteomic study on ovulatory PCOS serum samples using nano-LCMS/MS technique. Then, we analysed the proteomic profiles of substantially dysregulated proteins by projecting them onto protein interaction mapping and molecular network analysis software tools Gene Mania and STRING. We further investigated the involvement of the affected proteins in different PCOS-associated disorders and classified them through a review of the literature along with functional annotation software tools DAVID and Panther. We found a total of 228 proteins in serum; 109 were found in both ovulatory PCOS and controls, and 42 of those showed a difference of ≥twofold (19 higher in ovulatory PCOS and 23 lower). Among them, 35 proteins exhibited an association with the pathophysiological mechanisms underlying the manifestation of ovulatory PCOS manifestation and their correlations with PCOS-concurrent disorders were revealed. There were also 87 proteins that were only found in ovulatory PCOS and 32 that were only found in controls. We further highlighted significant functional hub molecules within protein interaction networks. Our findings indicated that the ovulatory PCOS involves a wide range of functional molecule derangements, which trigger aberrant biological responses and molecular interactions leading to the emergence of complications associated with ovulatory PCOS. Further omics studies are required to explain the different physiological mechanisms of the functional molecules contributing to the pathogenicity of this heterogeneous syndrome.</p></div>","PeriodicalId":793,"journal":{"name":"The Protein Journal","volume":"44 6","pages":"773 - 788"},"PeriodicalIF":1.4,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic In-Silico Insights into the Anti-quorum Sensing Potential of Coumaric Acid and Syringic Acid in Serratia marcescens with Invitro Analysis","authors":"Pooja Pandey,&nbsp;Bipin R. Shekhar,&nbsp;Dhanjit K. Das,&nbsp;Sirisha L. Vavilala","doi":"10.1007/s10930-025-10292-7","DOIUrl":"10.1007/s10930-025-10292-7","url":null,"abstract":"<div><p>Antibiotic resistance presents a major global health threat, especially with ESKAPE pathogens like <i>Serratia marcescens</i>, which exhibit resistance to all known antibiotics. Quorum sensing (QS) is key to its virulence and resistance, emphasizing the need for novel natural antimicrobial agents. This study investigates two plant-derived phenolic compounds, coumaric acid and syringic acid, targeting QS proteins of <i>S. marcescens</i> using <i>in silico</i> molecular docking, molecular dynamics simulations, and in vitro biochemical assays. Validated homology models of eight QS-associated proteins—BsmA, FimA, FimC, FlhD, LuxS, PigP, RsmA, and RssB—were employed for molecular docking studies, ADME (absorption, distribution, metabolism, and excretion) profiling, and 100-ns molecular dynamics (MD) simulations to evaluate ligand-binding stability. Coumaric acid displayed more desirable physicochemical properties (logP 1.79; TPSA 57.53 Ų) compared to syringic acid (logP 1.04; TPSA 75.99 Ų). Binding energy calculations indicated a stronger affinity of coumaric acid for six of the proteins, with the LuxS–coumaric acid complex showing the most significant interaction (ΔGbind − 21.74 ± 3.01 kcal/mol). Analysis of the MD trajectory revealed that coumaric acid enhanced protein stability, as shown by reductions in RMSF (root mean square fluctuation), a more compact Rg (radius of gyration), decreased SASA (solvent-accessible surface area), alterations in the Dictionary of secondary structure of protein (DSSP), and consistent hydrogen bonding. Conversely, syringic acid induced increased conformational flexibility and destabilized alpha-helices and beta-sheets in specific proteins. Principal component analysis (PCA) confirmed tighter conformational clustering in coumaric acid complexes, consistent with improved stabilization. Furthermore, antibacterial assays demonstrated strong inhibitory effects, with MIC values of 700 µg/mL for coumaric acid and 1000 µg/mL for syringic acid. Coumaric acid displayed a bactericidal effect, whereas syringic acid was bacteriostatic. Additionally, time–kill assays revealed a dose-dependent reduction in bacterial growth over 48 h following treatment with varying concentrations of these phenolic acids . Interestingly, qPCR analysis of QS-specific gene expression showed significant downregulation of key QS-regulated genes in response to both compounds, highlighting their potential as quorum-sensing inhibitors and supporting their development as alternative antimicrobial agents against antibiotic-resistant <i>S. marcescens</i>.</p></div>","PeriodicalId":793,"journal":{"name":"The Protein Journal","volume":"44 6","pages":"754 - 772"},"PeriodicalIF":1.4,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10930-025-10292-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the Phosphorylation Landscape of Anthrax Toxin Receptor 1: Mechanisms and Functional Insights Through Phosphoproteomic Data Integration","authors":"Ayadathil Sujina,&nbsp;Amal Fahma,&nbsp;Suhail Subair,&nbsp;Leona D’cunha,&nbsp;Athira Perunelly Gopalakrishnan,&nbsp;Samseera Ummar,&nbsp;Prathik Basthikoppa Shivamurthy,&nbsp;Poornima Ramesh,&nbsp;Rajesh Raju","doi":"10.1007/s10930-025-10293-6","DOIUrl":"10.1007/s10930-025-10293-6","url":null,"abstract":"<div><p>Anthrax Toxin Receptor 1 (ANTXR1) is a transmembrane protein involved in various biological processes, including angiogenesis, cell adhesion, and migration. As a receptor for Bacillus anthracis toxins and the oncolytic Seneca Valley virus, ANTXR1 plays pivotal roles in extracellular matrix interactions, actin cytoskeleton organization, and tumor progression. Despite its relevance in cancer biology, ANTXR1 remains understudied from a phosphoproteomics perspective. In this study, we report the phosphoproteomic landscape of the ANTXR1 protein through a unique data integration strategy from a mass spectrometry-based phosphoproteomics perspective. Through robust statistical analyses, conserved phosphorylation events of ANTXR1 across diverse experimental conditions were linked to its upstream kinases and binary interactors to deduce specific events modulated through ANTXR1 phosphorylation. This computational analysis of curated datasets identified conserved ANTXR1 phosphorylation events along with similar and oppositely co-regulated phosphorylation events of adjunct proteins, revealing extensive regulatory networks of ANTXR1. Our findings provide phosphorylation-dependent interaction between ANTXR1 and FLNA and their upstream kinases and phosphobinding motifs, emphasizing their collective role in cell migration. Overall, the study enhances the integrative analysis of mass spectrometry-based phosphoproteomics data through bioinformatics and statistical approaches.</p></div>","PeriodicalId":793,"journal":{"name":"The Protein Journal","volume":"44 6","pages":"742 - 753"},"PeriodicalIF":1.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145180787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}