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Revue canadienne de biologie experimentale最新文献

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[Disappearance of specific membrane components during reticulocyte maturation in sheep]. [绵羊网状细胞成熟过程中特定膜成分消失]。
Revue canadienne de biologie experimentale Pub Date : 1982-03-01
R M Johnstone, M Bin Tao Pan
{"title":"[Disappearance of specific membrane components during reticulocyte maturation in sheep].","authors":"R M Johnstone,&nbsp;M Bin Tao Pan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using antibodies prepared against whole reticulocyte suspensions, it is possible to identify specific membrane proteins from sheep reticulocytes. These membrane proteins can be isolated using the antibodies and Staphylococcus A protein to precipitate the immune complex. With fluorescein-labelled antireticulocyte antibody it may be shown that only reticulocytes and not red cells become fluorescent on exposure to the antibody, confirming that the antibody is directed against reticulocytes. Using either immobilized antibody columns or immobilized transferring columns, the same peptide bands from the membrane are retained by either column. The data suggest that the antibody is directed against the transferrin receptor. When the antibody is incubated for several hours with the reticulocytes, the cells release vesicles containing the transferrin receptor. This release is energy dependent since the process is inhibited by agents which interfere with metabolic activity.</p>","PeriodicalId":79252,"journal":{"name":"Revue canadienne de biologie experimentale","volume":"41 1","pages":"77-81"},"PeriodicalIF":0.0,"publicationDate":"1982-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18216222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[In vivo and in vitro effects of peptide extracts from Amanita virosa]. [毒伞菌肽提取物的体内外作用]。
Revue canadienne de biologie experimentale Pub Date : 1982-03-01
C Gicquaud, A Turcotte, J Gruda, B Tuchweber
{"title":"[In vivo and in vitro effects of peptide extracts from Amanita virosa].","authors":"C Gicquaud,&nbsp;A Turcotte,&nbsp;J Gruda,&nbsp;B Tuchweber","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cyclic peptides from the deadly mushroom Amanita virosa has been separated by methanolic extraction and chromatography on Sephadex. Three groups of peptides have been obtained: virotoxins, amaninamide and phalloidin. Virotoxins has been separated in two fractions named virotoxins A and virotoxins B. We have studied the properties of these two fractions of F actin in vitro and on mice in vivo. Our results show that virotoxins A and B protect F actin in vitro against chaotropic ions, depolymerization by DNAse I or cytochalasin B and heat denaturation. Virotoxins A and B increase the rate of polymerization of F actin. Virotoxins A and B are toxic compounds which produce hemorrhagic necrosis of liver that have been observed in detail by electron microscopy. In general, our in vitro and in vivo results show that virotoxins exhibit the same effect as phalloidin on F actin.</p>","PeriodicalId":79252,"journal":{"name":"Revue canadienne de biologie experimentale","volume":"41 1","pages":"23-34"},"PeriodicalIF":0.0,"publicationDate":"1982-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17347221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Control of lipid metabolism in experimental obesity]. [实验性肥胖的脂质代谢控制]
Revue canadienne de biologie experimentale Pub Date : 1982-03-01
N Bégin-Heick
{"title":"[Control of lipid metabolism in experimental obesity].","authors":"N Bégin-Heick","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Experimental obesity syndromes are characterized by considerable fat accretion which may be the result of hyperinsulinemia. Adipose tissue accretion may be the result both of the excessive synthesis of triglycerides and a defect in their mobilization from adipose tissue stores. The adenylate cyclase system which appears to be the site of the defect in mobilization is studies in more detail.</p>","PeriodicalId":79252,"journal":{"name":"Revue canadienne de biologie experimentale","volume":"41 1","pages":"83-90"},"PeriodicalIF":0.0,"publicationDate":"1982-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18216223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Hormonal control of liver gluconeogenesis]. [肝糖异生的激素控制]。
Revue canadienne de biologie experimentale Pub Date : 1982-03-01
L Hue
{"title":"[Hormonal control of liver gluconeogenesis].","authors":"L Hue","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The stimulation of gluconeogenesis by glucagon results from a concerted mechanism involving: 1) the stimulation of pyruvate transport and carboxylation in mitochondria; 2) the cyclic AMP dependent phosphorylation and inactivation of pyruvate kinase resulting in a re-routing of phosphoenolpyruvate towards glucose; 3) the inhibition of phosphofructokinase and the stimulation of fructose bisphosphatase resulting from the disappearance of fructose-2,6-bisphosphate. Catecholamines and vasopressin stimulate gluconeogenesis in starvation whereas in the fed state they promote glycogenolysis together with glycolysis.</p>","PeriodicalId":79252,"journal":{"name":"Revue canadienne de biologie experimentale","volume":"41 1","pages":"73-6"},"PeriodicalIF":0.0,"publicationDate":"1982-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17347222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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